JP6381525B2 - 組換え微生物およびその使用 - Google Patents
組換え微生物およびその使用 Download PDFInfo
- Publication number
- JP6381525B2 JP6381525B2 JP2015516270A JP2015516270A JP6381525B2 JP 6381525 B2 JP6381525 B2 JP 6381525B2 JP 2015516270 A JP2015516270 A JP 2015516270A JP 2015516270 A JP2015516270 A JP 2015516270A JP 6381525 B2 JP6381525 B2 JP 6381525B2
- Authority
- JP
- Japan
- Prior art keywords
- microorganism
- butanediol
- mek
- butanol
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000005700 microbiome Species 0.000 title description 220
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 claims description 386
- BTANRVKWQNVYAZ-UHFFFAOYSA-N butan-2-ol Chemical compound CCC(C)O BTANRVKWQNVYAZ-UHFFFAOYSA-N 0.000 claims description 315
- 150000007523 nucleic acids Chemical class 0.000 claims description 164
- 108020004707 nucleic acids Proteins 0.000 claims description 143
- 102000039446 nucleic acids Human genes 0.000 claims description 143
- 102000004190 Enzymes Human genes 0.000 claims description 140
- 108090000790 Enzymes Proteins 0.000 claims description 140
- 108090000623 proteins and genes Proteins 0.000 claims description 139
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 72
- 239000000758 substrate Substances 0.000 claims description 72
- 108010065027 Propanediol Dehydratase Proteins 0.000 claims description 65
- 101710088194 Dehydrogenase Proteins 0.000 claims description 64
- 241000894006 Bacteria Species 0.000 claims description 59
- 238000000034 method Methods 0.000 claims description 58
- 108010025885 Glycerol dehydratase Proteins 0.000 claims description 55
- 150000002009 diols Chemical class 0.000 claims description 52
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims description 46
- 102000004169 proteins and genes Human genes 0.000 claims description 36
- OWBTYPJTUOEWEK-QWWZWVQMSA-N (R,R)-butane-2,3-diol Chemical compound C[C@@H](O)[C@@H](C)O OWBTYPJTUOEWEK-QWWZWVQMSA-N 0.000 claims description 19
- 230000035772 mutation Effects 0.000 claims description 16
- 230000008569 process Effects 0.000 claims description 14
- 230000007420 reactivation Effects 0.000 claims description 11
- 239000001963 growth medium Substances 0.000 claims description 10
- 101710168863 Diacetyl reductase [(S)-acetoin forming] Proteins 0.000 claims description 9
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 241000588748 Klebsiella Species 0.000 claims description 2
- 101000933577 Klebsiella pneumoniae Diacetyl reductase [(S)-acetoin forming] Proteins 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 claims description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 description 138
- 230000014509 gene expression Effects 0.000 description 83
- 238000000855 fermentation Methods 0.000 description 75
- 230000004151 fermentation Effects 0.000 description 75
- 239000013598 vector Substances 0.000 description 71
- OWBTYPJTUOEWEK-UHFFFAOYSA-N (-)-(2R,3R)--2,3-butanediol Natural products CC(O)C(C)O OWBTYPJTUOEWEK-UHFFFAOYSA-N 0.000 description 70
- 238000004519 manufacturing process Methods 0.000 description 66
- OWBTYPJTUOEWEK-ZXZARUISSA-N meso-butane-2,3-diol Chemical compound C[C@@H](O)[C@H](C)O OWBTYPJTUOEWEK-ZXZARUISSA-N 0.000 description 64
- 238000006243 chemical reaction Methods 0.000 description 60
- 230000000694 effects Effects 0.000 description 47
- 239000000047 product Substances 0.000 description 47
- 229910002091 carbon monoxide Inorganic materials 0.000 description 43
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 41
- 239000007789 gas Substances 0.000 description 40
- 239000013612 plasmid Substances 0.000 description 40
- OWBTYPJTUOEWEK-WUCPZUCCSA-N (2s)-butane-2,3-diol Chemical compound CC(O)[C@H](C)O OWBTYPJTUOEWEK-WUCPZUCCSA-N 0.000 description 29
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 26
- ROWKJAVDOGWPAT-GSVOUGTGSA-N (R)-Acetoin Chemical compound C[C@@H](O)C(C)=O ROWKJAVDOGWPAT-GSVOUGTGSA-N 0.000 description 25
- 241001656809 Clostridium autoethanogenum Species 0.000 description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 22
- 241000193403 Clostridium Species 0.000 description 21
- 108060004795 Methyltransferase Proteins 0.000 description 20
- 230000012010 growth Effects 0.000 description 20
- OWBTYPJTUOEWEK-SYPWQXSBSA-N (3r)-butane-2,3-diol Chemical compound CC(O)[C@@H](C)O OWBTYPJTUOEWEK-SYPWQXSBSA-N 0.000 description 17
- 108090000765 processed proteins & peptides Proteins 0.000 description 16
- 108091028043 Nucleic acid sequence Proteins 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 14
- 230000011987 methylation Effects 0.000 description 14
- 238000007069 methylation reaction Methods 0.000 description 14
- 241000894007 species Species 0.000 description 14
- 102000016397 Methyltransferase Human genes 0.000 description 13
- 238000012239 gene modification Methods 0.000 description 13
- 230000005017 genetic modification Effects 0.000 description 13
- 235000013617 genetically modified food Nutrition 0.000 description 13
- 239000002609 medium Substances 0.000 description 13
- 239000000203 mixture Substances 0.000 description 13
- 239000000243 solution Substances 0.000 description 13
- 108020004414 DNA Proteins 0.000 description 12
- 150000001413 amino acids Chemical group 0.000 description 12
- 238000004128 high performance liquid chromatography Methods 0.000 description 12
- 230000003321 amplification Effects 0.000 description 11
- 230000015572 biosynthetic process Effects 0.000 description 11
- 230000001965 increasing effect Effects 0.000 description 11
- 238000003199 nucleic acid amplification method Methods 0.000 description 11
- 102000004196 processed proteins & peptides Human genes 0.000 description 11
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 10
- 108091029499 Group II intron Proteins 0.000 description 10
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 10
- 230000000813 microbial effect Effects 0.000 description 10
- 230000001105 regulatory effect Effects 0.000 description 10
- 230000009466 transformation Effects 0.000 description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 9
- 241000588724 Escherichia coli Species 0.000 description 9
- 101150028410 budC gene Proteins 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 238000003780 insertion Methods 0.000 description 9
- 230000037431 insertion Effects 0.000 description 9
- 229920001184 polypeptide Polymers 0.000 description 9
- 230000010076 replication Effects 0.000 description 9
- 108020004705 Codon Proteins 0.000 description 8
- 238000004520 electroporation Methods 0.000 description 7
- 239000013613 expression plasmid Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 6
- KAKZBPTYRLMSJV-UHFFFAOYSA-N Butadiene Chemical compound C=CC=C KAKZBPTYRLMSJV-UHFFFAOYSA-N 0.000 description 6
- 241000588749 Klebsiella oxytoca Species 0.000 description 6
- 229910000831 Steel Inorganic materials 0.000 description 6
- 239000008272 agar Substances 0.000 description 6
- 230000006801 homologous recombination Effects 0.000 description 6
- 238000002744 homologous recombination Methods 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 230000037361 pathway Effects 0.000 description 6
- 239000010959 steel Substances 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 108010092060 Acetate kinase Proteins 0.000 description 5
- 241000186566 Clostridium ljungdahlii Species 0.000 description 5
- 101100119095 Enterococcus faecalis (strain ATCC 700802 / V583) ermB gene Proteins 0.000 description 5
- 229930091371 Fructose Natural products 0.000 description 5
- 239000005715 Fructose Substances 0.000 description 5
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 5
- 241000588747 Klebsiella pneumoniae Species 0.000 description 5
- 108700023175 Phosphate acetyltransferases Proteins 0.000 description 5
- 238000011529 RT qPCR Methods 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 5
- 230000021615 conjugation Effects 0.000 description 5
- 230000002068 genetic effect Effects 0.000 description 5
- 230000001939 inductive effect Effects 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 239000012071 phase Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- OTVAEFIXJLOWRX-NXEZZACHSA-N thiamphenicol Chemical compound CS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CO)NC(=O)C(Cl)Cl)C=C1 OTVAEFIXJLOWRX-NXEZZACHSA-N 0.000 description 5
- 229960003053 thiamphenicol Drugs 0.000 description 5
- 239000002912 waste gas Substances 0.000 description 5
- ROWKJAVDOGWPAT-UHFFFAOYSA-N Acetoin Chemical compound CC(O)C(C)=O ROWKJAVDOGWPAT-UHFFFAOYSA-N 0.000 description 4
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 239000012298 atmosphere Substances 0.000 description 4
- AGOYDEPGAOXOCK-KCBOHYOISA-N clarithromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@](C)([C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)OC)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 AGOYDEPGAOXOCK-KCBOHYOISA-N 0.000 description 4
- 229960002626 clarithromycin Drugs 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000012790 confirmation Methods 0.000 description 4
- 238000010276 construction Methods 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 239000001257 hydrogen Substances 0.000 description 4
- 229910052739 hydrogen Inorganic materials 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 239000002207 metabolite Substances 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 238000010369 molecular cloning Methods 0.000 description 4
- 230000008685 targeting Effects 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- AXPZIVKEZRHGAS-UHFFFAOYSA-N 3-benzyl-5-[(2-nitrophenoxy)methyl]oxolan-2-one Chemical compound [O-][N+](=O)C1=CC=CC=C1OCC1OC(=O)C(CC=2C=CC=CC=2)C1 AXPZIVKEZRHGAS-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108091000080 Phosphotransferase Proteins 0.000 description 3
- KEOZKSCVXQEKDL-UHFFFAOYSA-N [C]=O.C(C)O Chemical compound [C]=O.C(C)O KEOZKSCVXQEKDL-UHFFFAOYSA-N 0.000 description 3
- 230000000789 acetogenic effect Effects 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 101150067366 adh gene Proteins 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical class CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 230000009483 enzymatic pathway Effects 0.000 description 3
- 238000002309 gasification Methods 0.000 description 3
- 108091008053 gene clusters Proteins 0.000 description 3
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 239000012139 lysis buffer Substances 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 102000020233 phosphotransferase Human genes 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- OWBTYPJTUOEWEK-IMJSIDKUSA-N (S,S)-butane-2,3-diol Chemical compound C[C@H](O)[C@H](C)O OWBTYPJTUOEWEK-IMJSIDKUSA-N 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- 241001468161 Acetobacterium Species 0.000 description 2
- 108010000700 Acetolactate synthase Proteins 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- 102100024630 Asc-type amino acid transporter 1 Human genes 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241001656810 Clostridium aceticum Species 0.000 description 2
- 241000445964 Clostridium autoethanogenum DSM 10061 Species 0.000 description 2
- 241001468167 Clostridium magnum Species 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- 108010053770 Deoxyribonucleases Proteins 0.000 description 2
- 102000016911 Deoxyribonucleases Human genes 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 201000008225 Klebsiella pneumonia Diseases 0.000 description 2
- 102100040604 Myotubularin-related protein 5 Human genes 0.000 description 2
- 108050003253 Myotubularin-related protein 5 Proteins 0.000 description 2
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 2
- 241000178986 Oxobacter Species 0.000 description 2
- 206010035717 Pneumonia klebsiella Diseases 0.000 description 2
- 101150081875 Slc7a10 gene Proteins 0.000 description 2
- 101150052859 Slc9a1 gene Proteins 0.000 description 2
- 108020004459 Small interfering RNA Proteins 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 108010084631 acetolactate decarboxylase Proteins 0.000 description 2
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 239000002551 biofuel Substances 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000006229 carbon black Substances 0.000 description 2
- 239000003245 coal Substances 0.000 description 2
- 239000000571 coke Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 238000003209 gene knockout Methods 0.000 description 2
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 2
- GFAZHVHNLUBROE-UHFFFAOYSA-N hydroxymethyl propionaldehyde Natural products CCC(=O)CO GFAZHVHNLUBROE-UHFFFAOYSA-N 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000002440 industrial waste Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 125000005647 linker group Chemical group 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 230000007483 microbial process Effects 0.000 description 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 2
- 230000002018 overexpression Effects 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 238000005504 petroleum refining Methods 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 238000010188 recombinant method Methods 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000013605 shuttle vector Substances 0.000 description 2
- 239000004055 small Interfering RNA Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000000527 sonication Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 241001515965 unidentified phage Species 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- ROWKJAVDOGWPAT-VKHMYHEASA-N (S)-Acetoin Chemical compound C[C@H](O)C(C)=O ROWKJAVDOGWPAT-VKHMYHEASA-N 0.000 description 1
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- YCPXWRQRBFJBPZ-UHFFFAOYSA-N 5-sulfosalicylic acid Chemical compound OC(=O)C1=CC(S(O)(=O)=O)=CC=C1O YCPXWRQRBFJBPZ-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 241001112695 Clostridiales Species 0.000 description 1
- 241000193163 Clostridioides difficile Species 0.000 description 1
- 241000193401 Clostridium acetobutylicum Species 0.000 description 1
- 241000193171 Clostridium butyricum Species 0.000 description 1
- 241001171821 Clostridium coskatii Species 0.000 description 1
- 241000272479 Clostridium diolis Species 0.000 description 1
- 241000193161 Clostridium formicaceticum Species 0.000 description 1
- 241001611023 Clostridium ragsdalei Species 0.000 description 1
- 241000186587 Clostridium scatologenes Species 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241001288713 Escherichia coli MC1061 Species 0.000 description 1
- 241000186394 Eubacterium Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241001251094 Formica Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108090001042 Hydro-Lyases Proteins 0.000 description 1
- 102000004867 Hydro-Lyases Human genes 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000178985 Moorella Species 0.000 description 1
- 241000193459 Moorella thermoacetica Species 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 229910020347 Na2WO3 Inorganic materials 0.000 description 1
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241000179039 Paenibacillus Species 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 101710182361 Pyruvate:ferredoxin oxidoreductase Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 241001394655 Roseburia inulinivorans Species 0.000 description 1
- 241000193448 Ruminiclostridium thermocellum Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241001138501 Salmonella enterica Species 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 108010034546 Serratia marcescens nuclease Proteins 0.000 description 1
- 101001010097 Shigella phage SfV Bactoprenol-linked glucose translocase Proteins 0.000 description 1
- 244000061457 Solanum nigrum Species 0.000 description 1
- 241000204388 Sporomusa Species 0.000 description 1
- 241000204376 Sporomusa ovata Species 0.000 description 1
- 241000217849 Sporomusa sphaeroides Species 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000004826 Synthetic adhesive Substances 0.000 description 1
- 241000186339 Thermoanaerobacter Species 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 241000193453 [Clostridium] cellulolyticum Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 108091006088 activator proteins Proteins 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 210000004507 artificial chromosome Anatomy 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 108010036467 butanediol dehydrogenase Proteins 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000007942 carboxylates Chemical class 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- VDQVEACBQKUUSU-UHFFFAOYSA-M disodium;sulfanide Chemical compound [Na+].[Na+].[SH-] VDQVEACBQKUUSU-UHFFFAOYSA-M 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000005431 greenhouse gas Substances 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 238000001597 immobilized metal affinity chromatography Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 239000004434 industrial solvent Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000000976 ink Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000000622 liquid--liquid extraction Methods 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000011880 melting curve analysis Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 description 1
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000005373 pervaporation Methods 0.000 description 1
- 239000003348 petrochemical agent Substances 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 235000011007 phosphoric acid Nutrition 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000005464 sample preparation method Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 description 1
- 229910001488 sodium perchlorate Inorganic materials 0.000 description 1
- 229910052979 sodium sulfide Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000012536 storage buffer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 229920003051 synthetic elastomer Polymers 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 239000005061 synthetic rubber Substances 0.000 description 1
- 125000000383 tetramethylene group Chemical group [H]C([H])([*:1])C([H])([H])C([H])([H])C([H])([H])[*:2] 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000018412 transposition, RNA-mediated Effects 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01004—R,R-butanediol dehydrogenase (1.1.1.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01028—Propanediol dehydratase (4.2.1.28)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/0103—Glycerol dehydratase (4.2.1.30)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261657292P | 2012-06-08 | 2012-06-08 | |
| US61/657,292 | 2012-06-08 | ||
| PCT/US2013/044865 WO2013185123A1 (en) | 2012-06-08 | 2013-06-09 | Recombinant microorganisms and uses therefor |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015518736A JP2015518736A (ja) | 2015-07-06 |
| JP2015518736A5 JP2015518736A5 (enExample) | 2016-08-04 |
| JP6381525B2 true JP6381525B2 (ja) | 2018-08-29 |
Family
ID=49712722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015516270A Active JP6381525B2 (ja) | 2012-06-08 | 2013-06-09 | 組換え微生物およびその使用 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9890384B2 (enExample) |
| EP (1) | EP2859089B1 (enExample) |
| JP (1) | JP6381525B2 (enExample) |
| KR (1) | KR102079275B1 (enExample) |
| CN (2) | CN113186144A (enExample) |
| IN (1) | IN2014DN10508A (enExample) |
| WO (1) | WO2013185123A1 (enExample) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130323820A1 (en) | 2012-06-01 | 2013-12-05 | Lanzatech New Zealand Limited | Recombinant microorganisms and uses therefor |
| KR102121888B1 (ko) | 2012-08-28 | 2020-06-12 | 란자테크 뉴질랜드 리미티드 | 재조합 미생물 및 이에 대한 용도 |
| CN112048525B (zh) | 2013-09-22 | 2024-03-12 | 朗泽科技新西兰有限公司 | 发酵工艺 |
| KR101577502B1 (ko) * | 2013-12-16 | 2015-12-14 | 지에스칼텍스 주식회사 | D(-)2,3-부탄디올의 생성능이 증강된 재조합 미생물 및 이를 이용한 d(-)2,3-부탄디올의 생산 방법 |
| KR102320074B1 (ko) | 2014-01-28 | 2021-11-03 | 란자테크 뉴질랜드 리미티드 | 재조합 미생물의 생산 방법 |
| KR101743021B1 (ko) | 2014-05-08 | 2017-06-02 | 한국생명공학연구원 | 재조합 미생물을 이용한 l형 2,3-부탄디올의 제조방법 |
| EP3210012B1 (en) | 2014-10-22 | 2023-06-07 | LanzaTech NZ, Inc. | Gas testing unit and method |
| JP6662868B2 (ja) | 2014-10-22 | 2020-03-11 | ランザテク・ニュージーランド・リミテッド | 複数ステージバイオリアクタープロセス |
| HUE052810T2 (hu) | 2014-12-08 | 2021-05-28 | Lanzatech New Zealand Ltd | Rekombináns mikroorganizmusok, amelyek megnövekedett fluxust mutatnak fermetációs útvonalon keresztül |
| US10131884B2 (en) | 2015-02-23 | 2018-11-20 | Lanzatech New Zealand Limited | Recombinant acetogenic bacterium for the conversion of methane to products |
| MY205861A (en) | 2015-10-13 | 2024-11-16 | Lanzatech Nz Inc | Genetically engineered bacterium comprising energy-generating fermentation pathway |
| ES2909579T3 (es) | 2015-12-03 | 2022-05-09 | Lanzatech Nz Inc | Arginina como única fuente de nitrógeno para microorganismos fijadores de C1 |
| US10358661B2 (en) | 2015-12-28 | 2019-07-23 | Lanzatech New Zealand Limited | Microorganism with modified hydrogenase activity |
| CN108603201A (zh) | 2016-02-01 | 2018-09-28 | 朗泽科技新西兰有限公司 | 整合发酵和电解工艺 |
| SG11201807025SA (en) * | 2016-02-26 | 2018-09-27 | Lanzatech New Zealand Ltd | Crispr/cas systems for c-1 fixing bacteria |
| KR102514023B1 (ko) | 2016-05-14 | 2023-03-23 | 란자테크, 인크. | 알데히드:페레독신 옥시도리덕타제 활성이 변경된 미생물 및 관련 방법 |
| ES2926336T3 (es) | 2017-09-08 | 2022-10-25 | Lanzatech Inc | Proceso para la producción de metabolitos utilizando sustratos que contienen C1 ricos en hidrógeno |
| WO2019126400A1 (en) * | 2017-12-19 | 2019-06-27 | Lanzatech, Inc. | Microorganisms and methods for the biological production of ethylene glycol |
| CN111683731B (zh) | 2018-02-12 | 2022-09-02 | 朗泽科技有限公司 | 用于提高碳转化效率的工艺 |
| ES2960999T3 (es) | 2018-04-20 | 2024-03-07 | Lanzatech Inc | Proceso de fermentación para producir etanol |
| EP3884060A4 (en) | 2018-11-19 | 2022-08-10 | Lanzatech, Inc. | FERMENTATION AND GASIFICATION INTEGRATION |
| CA3127942C (en) | 2019-01-29 | 2023-10-17 | Lanzatech, Inc. | Production of bio-based liquefied petroleum gas |
| KR20240135053A (ko) | 2019-02-08 | 2024-09-10 | 란자테크, 인크. | 유사한 비등점 생성물의 회수 방법 |
| WO2020188033A1 (en) | 2019-03-20 | 2020-09-24 | Global Bioenergies | Improved means and methods for producing isobutene from acetyl-coa |
| KR20240158377A (ko) | 2019-07-11 | 2024-11-04 | 란자테크, 인크. | 가스 이용률을 최적화하는 방법 |
| US11932818B2 (en) | 2020-03-16 | 2024-03-19 | Lanzatech, Inc. | Tail gas of gas fermentation to dry gasification feedstock |
| JP2023518045A (ja) | 2020-03-18 | 2023-04-27 | ランザテク,インコーポレイテッド | ガス基質からの2-フェニルエタノールの発酵産生 |
| US12134794B2 (en) | 2020-04-29 | 2024-11-05 | Lanzatech, Inc. | Fermentative production of B-ketoadipate from gaseous substrates |
| KR20230156819A (ko) | 2020-06-06 | 2023-11-14 | 란자테크, 인크. | 아세토락테이트 탈카복실화효소 유전자위에 녹인이 있는 미생물 |
| US11788092B2 (en) | 2021-02-08 | 2023-10-17 | Lanzatech, Inc. | Recombinant microorganisms and uses therefor |
| JP2024513395A (ja) | 2021-04-09 | 2024-03-25 | ランザテク,インコーポレイテッド | 供給原料を提供するためのプロセス及び装置 |
| WO2023004293A1 (en) | 2021-07-20 | 2023-01-26 | Lanzatech, Inc. | Recombinant microorganisms and uses therefor |
| TW202307202A (zh) | 2021-08-06 | 2023-02-16 | 美商朗澤科技有限公司 | 用於改良乙二醇之生物產生的微生物及方法 |
| US12091648B2 (en) | 2021-11-03 | 2024-09-17 | Lanzatech, Inc. | System and method for generating bubbles in a vessel |
| US12280331B2 (en) | 2022-04-29 | 2025-04-22 | Lanzatech, Inc. | Low residence time gas separator |
| US12077800B2 (en) | 2022-06-16 | 2024-09-03 | Lanzatech, Inc. | Liquid distributor system and process of liquid distribution |
| EP4544062A1 (en) | 2022-06-21 | 2025-04-30 | Lanzatech, Inc. | Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from c1-substrates |
| WO2023250382A1 (en) | 2022-06-21 | 2023-12-28 | Lanzatech, Inc. | Microorganisms and methods for the continuous co-production of tandem repeat proteins and chemical products from c1-substrates |
| US12129503B2 (en) | 2022-08-10 | 2024-10-29 | Lanzatech, Inc. | Carbon sequestration in soils with production of chemical products |
| KR20240024407A (ko) * | 2022-08-16 | 2024-02-26 | 한국과학기술원 | 일산화탄소 이용능이 증대된 미생물 및 이를 이용한 2,3-bdo 생산 |
| US12281344B2 (en) | 2023-06-05 | 2025-04-22 | Lanzatech, Inc. | Integrated gas fermentation |
| WO2024253883A1 (en) | 2023-06-05 | 2024-12-12 | Lanzatech, Inc. | Integrated gas fermentation and carbon black processes |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5173429A (en) | 1990-11-09 | 1992-12-22 | The Board Of Trustees Of The University Of Arkansas | Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism |
| US5593886A (en) | 1992-10-30 | 1997-01-14 | Gaddy; James L. | Clostridium stain which produces acetic acid from waste gases |
| UA72220C2 (uk) | 1998-09-08 | 2005-02-15 | Байоенджініерінг Рісорсиз, Інк. | Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання |
| DE60121335T2 (de) | 2000-07-25 | 2007-08-02 | Emmaus Foundation, Inc., Fayetteville | Verfahren zur steigerung der ethanolproduktion bei der mikrobiellen fermentation |
| ZA200807719B (en) * | 2006-05-02 | 2009-12-30 | Du Pont | Fermentive production of four carbon alcohols |
| US8962298B2 (en) * | 2006-05-02 | 2015-02-24 | Butamax Advanced Biofuels Llc | Recombinant host cell comprising a diol dehydratase |
| US7704723B2 (en) | 2006-08-31 | 2010-04-27 | The Board Of Regents For Oklahoma State University | Isolation and characterization of novel clostridial species |
| US20080274522A1 (en) * | 2007-05-02 | 2008-11-06 | Bramucci Michael G | Method for the production of 2-butanone |
| FR2916000B1 (fr) * | 2007-05-07 | 2013-07-05 | Lallemand Sas | Moyens pour reduire l'accumulation d'acetoine dans des milieux de fermentation alcoolique |
| CN102876609A (zh) | 2007-11-13 | 2013-01-16 | 兰扎泰克新西兰有限公司 | 新型菌及其使用方法 |
| US8372612B2 (en) * | 2007-12-21 | 2013-02-12 | Butamax(Tm) Advanced Biofuels Llc | Production of four carbon alcohols using improved strain |
| AU2009258344B2 (en) | 2008-06-09 | 2013-11-07 | Lanzatech Nz, Inc. | Production of butanediol by anaerobic microbial fermentation |
| EP2337869B1 (en) * | 2008-09-29 | 2015-08-19 | Butamax Advanced Biofuels Llc | Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria |
| US20100184173A1 (en) * | 2008-11-14 | 2010-07-22 | Genomatica, Inc. | Microorganisms for the production of methyl ethyl ketone and 2-butanol |
| US8039239B2 (en) * | 2008-12-16 | 2011-10-18 | Coskata, Inc. | Recombinant microorganisms having modified production of alcohols and acids |
| AU2009327490A1 (en) * | 2008-12-16 | 2011-07-28 | Genomatica, Inc. | Microorganisms and methods for conversion of syngas and other carbon sources to useful products |
| US9260708B2 (en) * | 2009-09-29 | 2016-02-16 | Butamax Advanced Biofuels Llc | Yeast production host cells |
| US8143037B2 (en) | 2010-03-19 | 2012-03-27 | Coskata, Inc. | Ethanologenic Clostridium species, Clostridium coskatii |
| WO2012034023A2 (en) * | 2010-09-10 | 2012-03-15 | University Of Delaware | Recombinant clostridia that fix co2 and co and uses thereof |
| US20110236941A1 (en) * | 2010-10-22 | 2011-09-29 | Lanzatech New Zealand Limited | Recombinant microorganism and methods of production thereof |
| ES2702621T3 (es) | 2011-02-25 | 2019-03-04 | Lanzatech New Zealand Ltd | Microorganismos recombinantes y sus usos |
| WO2014092562A1 (en) * | 2012-12-11 | 2014-06-19 | Photanol B.V. | Synthesis of acetoin, 2,3-butanediol and 2-butanol by cyanobacteria by heterologous expression of a catabolic pathway |
-
2013
- 2013-06-09 JP JP2015516270A patent/JP6381525B2/ja active Active
- 2013-06-09 CN CN202110477268.4A patent/CN113186144A/zh active Pending
- 2013-06-09 WO PCT/US2013/044865 patent/WO2013185123A1/en not_active Ceased
- 2013-06-09 EP EP13800454.4A patent/EP2859089B1/en active Active
- 2013-06-09 KR KR1020147036648A patent/KR102079275B1/ko active Active
- 2013-06-09 CN CN201380042293.3A patent/CN104919037A/zh active Pending
- 2013-06-10 US US13/914,234 patent/US9890384B2/en active Active
-
2014
- 2014-12-09 IN IN10508DEN2014 patent/IN2014DN10508A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015518736A (ja) | 2015-07-06 |
| IN2014DN10508A (enExample) | 2015-08-21 |
| CN104919037A (zh) | 2015-09-16 |
| EP2859089A4 (en) | 2015-12-30 |
| KR20150020619A (ko) | 2015-02-26 |
| EP2859089A1 (en) | 2015-04-15 |
| KR102079275B1 (ko) | 2020-02-19 |
| WO2013185123A1 (en) | 2013-12-12 |
| EP2859089B1 (en) | 2017-03-22 |
| US9890384B2 (en) | 2018-02-13 |
| CN113186144A (zh) | 2021-07-30 |
| US20130330809A1 (en) | 2013-12-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6381525B2 (ja) | 組換え微生物およびその使用 | |
| JP6199747B2 (ja) | 組換え微生物およびそれらの使用 | |
| JP6445970B2 (ja) | 組換え微生物およびその使用 | |
| JP6014042B2 (ja) | 組換え微生物による一酸化炭素からのブタノールの産生 | |
| JP6302471B2 (ja) | 組換え微生物およびその使用 | |
| US9834795B2 (en) | Recombinant microorganisms and uses therefor | |
| JP6512825B2 (ja) | 組換え微生物およびその使用方法 | |
| US9365868B2 (en) | Fermentation process for producing isopropanol using a recombinant microorganism | |
| JP2016520325A (ja) | 発酵経路を通る流束の増加を示す、組換え微生物 | |
| NZ614459B2 (en) | Recombinant microorganisms and uses therefor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150106 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160510 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20160510 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160603 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20160603 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20170321 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20170322 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20170620 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20170821 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170920 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20180227 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180525 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20180703 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20180731 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6381525 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| S533 | Written request for registration of change of name |
Free format text: JAPANESE INTERMEDIATE CODE: R313533 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |