JP6339548B2 - 微細藻類における異種ポリペプチド、微細藻類細胞外体、組成物の産生、ならびにそれらの作製および使用の方法 - Google Patents

微細藻類における異種ポリペプチド、微細藻類細胞外体、組成物の産生、ならびにそれらの作製および使用の方法 Download PDF

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JP6339548B2
JP6339548B2 JP2015206158A JP2015206158A JP6339548B2 JP 6339548 B2 JP6339548 B2 JP 6339548B2 JP 2015206158 A JP2015206158 A JP 2015206158A JP 2015206158 A JP2015206158 A JP 2015206158A JP 6339548 B2 JP6339548 B2 JP 6339548B2
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protein
membrane
extracellular body
schizochytrium
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JP2016047055A (ja
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アン−セシル ブイ. バイン
アン−セシル ブイ. バイン
ジェイムズ ケーシー リップマイアー
ジェイムズ ケーシー リップマイアー
カーク エミル アプト
カーク エミル アプト
ロス エリック ザークレ
ロス エリック ザークレ
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サノフィ ワクチン テクノロジーズ エス.エー.エス.
サノフィ ワクチン テクノロジーズ エス.エー.エス.
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JP2015206158A 2009-12-28 2015-10-20 微細藻類における異種ポリペプチド、微細藻類細胞外体、組成物の産生、ならびにそれらの作製および使用の方法 Active JP6339548B2 (ja)

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Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI350854B (en) 2001-04-16 2011-10-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
TWI504749B (zh) * 2009-03-16 2015-10-21 Dsm Ip Assets Bv 於網黏菌門微生物中生產蛋白質之技術
CA2785653C (en) * 2009-12-28 2018-05-01 Merial Limited Recombinant ndv antigen and uses thereof
CN103068984B (zh) * 2009-12-28 2017-12-01 Dsm Ip资产公司 微藻中血凝素‑神经氨酸酶蛋白的生产
EP2444495A1 (en) * 2010-10-20 2012-04-25 Algenics Secretion of recombinant polypeptides in the extracellular medium of diatoms
CN107630017A (zh) * 2011-03-07 2018-01-26 Dsm营养产品股份公司 工程化破囊壶菌属微生物
US9404101B2 (en) * 2011-08-24 2016-08-02 Novozymes, Inc. Methods for obtaining positive transformants of a filamentous fungal host cell
CA2879680A1 (en) * 2012-07-24 2014-01-30 Bp Corporation North America Inc. Xylose isomerases and their uses
WO2014045191A2 (en) * 2012-09-18 2014-03-27 Myko Tech Private Limited Extracellular polysaccharides from labyrinthulomycetes with broad-spectrum antiviral activities
EP3200606B1 (de) * 2014-10-02 2021-03-31 Evonik Operations GmbH Verfahren zur herstellung eines pufas enthaltenden futtermittels durch extrusion einer pufas enthaltenden biomasse des typs labyrinthulomycetes
EP3200603A1 (de) 2014-10-02 2017-08-09 Evonik Degussa GmbH Pufas enthaltendes futtermittel mit hoher abriebfestigkeit und hoher wasserstabilität
US11324234B2 (en) 2014-10-02 2022-05-10 Evonik Operations Gmbh Method for raising animals
ES2870093T3 (es) 2014-10-02 2021-10-26 Evonik Operations Gmbh Biomasa que contiene PUFA con estabilidad celular elevada y su empleo para la producción de piensos
EP3265567B1 (en) 2015-03-02 2020-07-29 Conagen Inc. Regulatory elements from labyrinthulomycetes microorganisms
AU2016293487B2 (en) 2015-07-13 2021-07-01 MARA Renewables Corporation Enhancing microalgal metabolism of xylose
FR3038913B1 (fr) * 2015-07-17 2020-05-01 Fermentalg Biomasse de thraustochytrides, procede de culture et utilisations
US20190216857A1 (en) * 2016-09-09 2019-07-18 Cornell University Delivery of nucleic acids, proteins and small molecules in vitreous vesicular bodies
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
EP3544994B1 (en) 2016-11-27 2024-09-25 Triton Algae Innovations, Inc. Method of purification of recombinant osteopontin from microalgae
CN111954713A (zh) * 2018-03-05 2020-11-17 科纳根公司 用于产生具有低硫酸化的糖分子的生物体和方法
WO2020004144A1 (ja) 2018-06-27 2020-01-02 住友電気工業株式会社 フレキシブルプリント配線板の原形体、フレキシブルプリント配線板の製造方法、集光型太陽光発電モジュール、及び、発光モジュール
US11752175B2 (en) 2018-09-21 2023-09-12 Cornell University Compositions and methods for glaucoma
CN109161576B (zh) * 2018-09-26 2020-08-07 武汉中科光谷绿色生物技术有限公司 促进枯草芽孢杆菌发酵生产n-乙酰神经氨酸的方法
WO2021100642A1 (ja) * 2019-11-21 2021-05-27 パナソニックIpマネジメント株式会社 改変シアノバクテリア、改変シアノバクテリアの製造方法、及び、タンパク質の製造方法
IT201900024580A1 (it) 2019-12-18 2021-06-18 Consiglio Nazionale Ricerche Vescicole extracellulari da microalghe
CN111363045B (zh) * 2020-02-18 2021-12-17 厦门大学 一种流感、hiv嵌合蛋白及嵌合病毒疫苗的制备方法
CN111621471B (zh) * 2020-06-12 2022-03-11 成都世联康健生物科技有限公司 一种软组织胞外囊泡的提取方法及应用
CN112452506A (zh) * 2020-11-06 2021-03-09 五原县沃丰生物科技有限责任公司 一种培养基粉末的生产方法
US11175293B1 (en) 2021-01-04 2021-11-16 University Of Utah Research Foundation Rapid assay for detection of SARS-CoV-2 antibodies
US20240287443A1 (en) * 2021-06-24 2024-08-29 Reliance Industries Limited Recombinant algae and production of spider silk protein from the recombinant algae
CN113698451B (zh) * 2021-08-02 2023-04-11 广东海洋大学 一种微藻多肽及其制备方法和应用
EP4426346A1 (en) 2021-11-05 2024-09-11 Sanofi Pasteur, Inc. Multivalent influenza vaccines comprising recombinant hemagglutinin and neuraminidase and methods of using the same
CN118302190A (zh) 2021-11-05 2024-07-05 赛诺菲 包含血凝素和神经氨酸酶的杂合多价流感疫苗及其使用方法
CA3239417A1 (en) 2021-11-30 2023-06-08 Yvonne CHAN Human metapneumovirus vaccines
WO2023232976A1 (en) * 2022-06-03 2023-12-07 Ags Therapeutics Sas Extracellular vesicles from genetically-modified microalgae containing endogenously-loaded cargo, their preparation, and uses
EP4525900A1 (en) 2022-06-14 2025-03-26 Hun-Ren Támogatott Kutatócsoportok Irodája Extracellular vesicles for use in therapy
CN115992052A (zh) * 2022-11-18 2023-04-21 暨南大学 一种微藻类外泌体、其制备方法和应用
JP7546136B1 (ja) 2023-12-27 2024-09-05 ロート製薬株式会社 微細藻類由来の細胞外小胞を含有する組成物

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4659669A (en) * 1981-03-02 1987-04-21 Regents Of The Univ. Of California Microbial expression of human influenza hemagglutinin proteins
US4752473A (en) * 1984-10-12 1988-06-21 The Regents Of The University Of California Expression of glycosylated human influenza hemagglutinin proteins
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5762939A (en) * 1993-09-13 1998-06-09 Mg-Pmc, Llc Method for producing influenza hemagglutinin multivalent vaccines using baculovirus
PT733113E (pt) * 1994-01-11 2007-08-10 Vlaams Interuniv Inst Biotech Vacina contra a gripe
US5824309A (en) * 1995-12-05 1998-10-20 University Of Massachusetts Recombinant gas vesicles and uses thereof
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
KR20000075076A (ko) * 1999-05-28 2000-12-15 최태진 형질전환된 미세조류를 이용하여 외래 단백질을 생산하는 방법
DK1294892T3 (da) * 2000-06-23 2008-01-28 Wyeth Corp Aggregering af vildtype- og kimære influenzaviruslignende partikler (VLP'er)
US20060029604A1 (en) 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US20060257399A1 (en) 2000-06-28 2006-11-16 Glycofi, Inc. Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform
EP2322644A1 (en) 2000-06-28 2011-05-18 GlycoFi, Inc. Methods for producing modified glycoproteins
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
TWI350854B (en) * 2001-04-16 2011-10-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
AU2007249124B2 (en) * 2001-04-16 2012-05-17 Dsm Ip Assets B.V. Product and process for transformation of thraustochytriales microorganisms
AU2003213060A1 (en) * 2002-02-14 2003-09-04 Novavax, Inc. Optimization of gene sequences of chimeric virus-like particles for expression in insect cells
WO2004042001A2 (en) * 2002-05-17 2004-05-21 Emory University Virus-like particles, methods of preparation, and immonogenic compositions
US7332299B2 (en) * 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
WO2005047501A1 (en) * 2003-02-24 2005-05-26 Johns Hopkins University Molecular vaccines employing nucleic acid encoding anti-apoptotic proteins
AU2004265232A1 (en) * 2003-06-05 2005-02-24 Wyeth Holdings Corporation Immunogenic compositions comprising venezuelan equine encephalitis virus replicon vectors and paramyxovirus protein antigens
WO2005116258A2 (en) * 2004-05-25 2005-12-08 Medimmune Vaccines, Inc. Influenza hemagglutinin and neuraminidase varians
US8936930B2 (en) * 2004-08-05 2015-01-20 Ben-Gurion University Of The Negev Research And Development Authority Red microalgae expressing exogenous polypeptides and methods of generating and utilizing same
WO2006073970A2 (en) * 2005-01-06 2006-07-13 The Johns Hopkins University Rna interference that blocks expression of pro-apoptotic proteins potentiates immunity induced by dna and transfected dendritic cell vaccines
JP4796786B2 (ja) * 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類を形質転換可能なベクター
JP4796787B2 (ja) 2005-04-28 2011-10-19 富士フイルム株式会社 ラビリンチュラ類への遺伝子導入法
US9216212B2 (en) * 2005-08-05 2015-12-22 University Of Massachusetts Virus-like particles as vaccines for paramyxovirus
US20070042001A1 (en) * 2005-08-16 2007-02-22 Hawaii Biotech, Inc. Influenza recombinant subunit vaccine
EP1991571A2 (en) * 2006-02-16 2008-11-19 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Antiviral agents and vaccines against influenza
CA2638760A1 (en) * 2006-03-07 2007-09-13 Vaxinnate Corporation Compositions that include hemagglutinin, methods of making and methods of use thereof
EP2004835A4 (en) * 2006-03-15 2011-02-23 Martek Biosciences Corp MERHFACH UNSATURATED FATTY ACIDS CONTAINING PLANT SEA OILS
WO2007130327A2 (en) * 2006-05-01 2007-11-15 Technovax, Inc. Influenza virus-like particle (vlp) compositions
AU2007345682B2 (en) * 2006-07-27 2013-07-18 Ligocyte Pharmaceuticals, Inc. Chimeric virus-like particles
US20100330105A1 (en) * 2006-08-22 2010-12-30 John Hopkins University Anticancer Combination Therapies
CA2615372A1 (en) * 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
EP2238253B1 (en) * 2007-11-27 2012-09-12 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
EP2090648A1 (en) * 2008-02-12 2009-08-19 Institut Francais de Recherche pour l'Exploitation de la Mere(Ifremer) Production of glycosylated polypeptides in microalgae
KR20090096890A (ko) * 2008-03-10 2009-09-15 전남대학교산학협력단 대장균에서 조류인플루엔자 바이러스의 뉴라미니다제 n1을발현하기 위한 벡터 및 방법, 이의 사용방법, 및뉴라미니다제 저해제
FR2928926B1 (fr) * 2008-03-18 2015-08-21 Centre Nat Rech Scient Polynucleotides et polypeptides chimeriques permettant la secretion d'un polypeptide d'interet en association avec des exosomes et leur utilisation pour la production de compositions immunogenes
EP2260010A4 (en) * 2008-03-19 2012-07-11 Momentive Specialty Chemicals Res Belgium ADJUVANT FOR CONCRETE AND CEMENT FORMULATIONS AND PROCESSES FOR PREPARING THE SAME
TWI504749B (zh) 2009-03-16 2015-10-21 Dsm Ip Assets Bv 於網黏菌門微生物中生產蛋白質之技術
CN103068984B (zh) * 2009-12-28 2017-12-01 Dsm Ip资产公司 微藻中血凝素‑神经氨酸酶蛋白的生产
CA2785653C (en) * 2009-12-28 2018-05-01 Merial Limited Recombinant ndv antigen and uses thereof

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