JP6312688B2 - サワードウへのグルテン分解用のマイクロカプセル化細菌集団及びそのサワードウの製造方法 - Google Patents
サワードウへのグルテン分解用のマイクロカプセル化細菌集団及びそのサワードウの製造方法 Download PDFInfo
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- JP6312688B2 JP6312688B2 JP2015540221A JP2015540221A JP6312688B2 JP 6312688 B2 JP6312688 B2 JP 6312688B2 JP 2015540221 A JP2015540221 A JP 2015540221A JP 2015540221 A JP2015540221 A JP 2015540221A JP 6312688 B2 JP6312688 B2 JP 6312688B2
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Description
a)各乳酸菌株を別々に再活性化するステップと、
b)活性化された各菌株を、各菌株が所望の濃度となるまで別々に液体培地で培養するステップと、
c)各菌株に対して、微生物を集めるために余分の培地を好ましくは遠心分離で除去し、ペレットを得るステップと、
d)各菌株に対して得られたペレットを別々に再懸濁させて、生食懸濁液とし、所要の体積に調整するステップと、
e)所要量の各菌株を混合し、最終体積に仕上げるステップと、
f)20〜30%の溶解固形物となるようにカプセル化剤を適切な割合で水に溶かすステップと、
g)プレバイオティクスとトレハロースを添加するステップと、
h)3つの乳酸菌株の混合物が1グラムの粉末のマイクロカプセル当り1010CFU程度となるよう、3つの乳酸菌株の混合物を接種するステップと、
i)入口温度が110〜160℃、出口温度が60〜80℃、送り速度が20〜50mL/minで、好ましくは噴霧乾燥器で乾燥するステップとを含む。
i)30〜60%の粉に40〜80%の水を混合し、パン生地の収量が150〜475、好ましくは150〜160のパン生地を得るステップと、
ii)混練水に事前に溶かした、細菌由来のタンパク質分解酵素0.005〜0.03%及び真菌由来のタンパク質分解酵素0.001〜0.02%を添加するステップと、
iii)3つの乳酸菌株の混合物が1グラムの粉当り1010CFU程度となるよう、マイクロカプセル化細菌集団を添加するステップと、
iv)混合するステップと、
v)30〜37℃の温度と70〜92%、好ましくは75〜80%の相対湿度で、3〜48時間、好ましくは28〜35時間発酵させるステップとを含む。
接種源の生産
ラクトバチルスプランタルムATCC8014、ラクトバチルスサンフランシセンシスATCC14869、及びラクトバチルスブレビスATCC27652の菌株をATCCから業者を通して入手した。ラクトバチルスプランタルムATCC8014とラクトバチルスサンフランシセンシスATCC14869は固体培地にて保存され、ラクトバチルスブレビスATCC27652は凍結乾燥された。
細菌集団のマイクロカプセル化
酸素に対する遮断性のために、タンパク質を90%含む乳清から分離したタンパク質と、デキストロース当量が10のマルトデキストリンと、アラビアゴムとを、それぞれの割合でカプセル化剤として選択した。
乳酸菌の発達に好ましいプレバイオティクス比率の決定
上述のように、マイクロカプセル化集団へのポリデキストロース(P)、イヌリン(I)、及びアガベシロップ(M)の添加を評価した。そこで、表1で示すように、本実験ではシンプレックス・セントロイド混合計画法を用いた。
サワードウの試作
手作りのパン焼きに適した、市販の白い小麦粉と、1010CFU/gの濃度で実施例2によって得られたマイクロカプセル化細菌集団のほかに、カプセル化剤と、トレハロースと、プレバイオティクスとしてのアガベシロップとを用いた。
アストルガのカップケーキ型の菓子パンをサワードウから乳酸発酵によって製造
実施例4によって得られたサワードウを使用して、カップケーキ型のグルテンフリーの菓子パンを製造した。バターと、マーガリンと、飴ガラス(sugar glass)とを、ミキサーのパレットを使って混ぜた。まず、混ぜ合わさるまで低速で混ぜ、その後速度を上げ、混合物に空気をなるべく多く含めるように更に20分混ぜた。
消費者によるアストルガのカップケーキの評価
従来の生地で作られた、いわゆる無添加のカップケーキに対して、実施例5(本発明のマイクロカプセル化微生物集団とタンパク質分解酵素の組み合わせによって得られたサワードウを使用)によって調理したオレンジと、ライムと、チョコレート味のカップケーキの認容性を調査するため、消費者による評価を行った。
Claims (20)
- グルテン分解用のマイクロカプセル化細菌集団であって、
a)ラクトバチルスプランタルムATCC8014、ラクトバチルスサンフランシセンシスATCC27652、及びラクトバチルスブレビスATCC14869と、b)カプセル化剤と、c)プレバイオティクスと、d)トレハロースとを含み、 細菌由来のタンパク質分解酵素及び真菌由来のタンパク質分解酵素と組み合わせられることを特徴とするグルテン分解用のマイクロカプセル化細菌集団。 - 前記カプセル化剤が、タンパク質を90%含む乳清から分離したタンパク質、デキストロース当量が10のマルトデキストリン、アラビアゴム、メスキートゴム、アルギン酸ナトリウム、ペクチン、大豆から分離したタンパク質、カゼイン、及びこれらの組み合わせからなる群より選択されることを特徴とする、請求項1に記載のマイクロカプセル化細菌集団。
- 前記カプセル化剤が、タンパク質を90%含む乳清から分離したタンパク質、デキストロース当量が10のマルトデキストリン、アラビアゴム、及びこれらの組み合わせであることを特徴とする、請求項2に記載のマイクロカプセル化細菌集団。
- 前記プレバイオティクスが、ポリデキストロース、イヌリン、アガベシロップからなる群より選択されることを特徴とする、請求項1に記載のマイクロカプセル化細菌集団。
- 前記プレバイオティクスが、アガベシロップであることを特徴とする、請求項4に記載のマイクロカプセル化細菌集団。
- 前記細菌由来のタンパク質分解酵素及び前記真菌由来のタンパク質分解酵素が、通常パン焼きに使われる酵素より選択されることを特徴とする、請求項1に記載のマイクロカプセル化細菌集団。
- a)ラクトバチルスプランタルムATCC8014と、b)ラクトバチルスサンフランシセンシスATCC27652と、c)ラクトバチルスブレビスATCC14869と、d)タンパク質を90%含む乳清から分離したタンパク質と、e)デキストロース当量が10のマルトデキストリンと、f)アラビアゴムと、g)アガベシロップと、h)トレハロースとを含み、
細菌由来のタンパク質分解酵素及び真菌由来のタンパク質分解酵素と組み合わせられることを特徴とする、請求項1に記載のマイクロカプセル化細菌集団。 - a)ラクトバチルスプランタルムATCC8014と、b)ラクトバチルスサンフランシセンシスATCC27652と、c)ラクトバチルスブレビスATCC14869と、d)タンパク質を90%含む乳清から分離したタンパク質を40〜60%と、e)デキストロース当量が10のマルトデキストリンを10〜20%と、f)アラビアゴムを20〜80%と、g)アガベシロップを1〜10%と、h)トレハロースとを含み、
細菌由来のタンパク質分解酵素0.005〜0.03%及び真菌由来のタンパク質分解酵素0.001〜0.02%と組み合わせられることを特徴とする、請求項7に記載のマイクロカプセル化細菌集団。 - 請求項1に記載のグルテン分解用のマイクロカプセル化細菌集団を得る方法であって、
a)各乳酸菌株を別々に再活性化するステップと、
b)活性化された各菌株を、各菌株が所望の濃度となるまで別々に液体培地で培養するステップと、
c)各菌株に対して、余分の培地を除去し、微生物を集めて、ペレットを得るステップと、
d)各菌株に対して得られたペレットを別々に再懸濁させて生食懸濁液とし、所要の体積に調整するステップと、
e)所要量の各菌株を混合し、最終体積に仕上げるステップと、
f)カプセル化剤を適切な割合で水に溶かして、溶解固形物を20〜30%とするステップと、
g)プレバイオティクスとトレハロースを添加するステップと、
h)前記3つの乳酸菌株の混合物を接種して、前記3つの乳酸菌株の混合物を1グラムの粉末のマイクロカプセル当り1010CFU程度とするステップと、
i)入口温度が110〜160℃、出口温度が60〜80℃、送り速度が20〜50mL/minで、噴霧乾燥するステップとを含むことを特徴とする、方法。 - 前記噴霧乾燥が、入口温度が150℃、出口温度が80℃、送り速度が22mL/minで、噴霧乾燥器で行われることを特徴とする、請求項9に記載のマイクロカプセル化細菌集団を得る方法。
- グルテン濃度が20ppm未満であるサワードウを得るための、請求項1に記載のマイクロカプセル化細菌集団と、細菌由来のタンパク質分解酵素及び真菌由来のタンパク質分解酵素との組み合わせの使用。
- 前記サワードウのグルテン濃度が10ppm未満であることを特徴とする、請求項11に記載の使用。
- 20ppm未満の前記グルテン濃度が、少なくとも3時間の発酵後に得られることを特徴とする、請求項11に記載の使用。
- 請求項1に記載のグルテン分解用のマイクロカプセル化細菌集団と、細菌由来のタンパク質分解酵素及び真菌由来のタンパク質分解酵素との組み合わせからサワードウを製造する方法であって、
i)30〜60%の粉に40〜80%の水を混合し、パン生地の収量が150〜475のパン生地を得るステップと、
ii)事前に混練水に溶かした、細菌由来のタンパク質分解酵素0.005〜0.03%及び真菌由来のタンパク質分解酵素0.001〜0.02%を添加するステップと、
iii)該マイクロカプセル化細菌集団を1グラムの粉当り1010CFU程度となるように添加するステップと、
iv)混合するステップと、
v)30〜37℃の温度と70〜92%の相対湿度で、3〜48時間発酵させるステップとを含むことを特徴とする、方法。 - ステップi)で使用される前記粉が、小麦粉、ライ麦粉、及びオーツ麦粉から選択されることを特徴とする、請求項14に記載のサワードウの製造方法。
- 前記粉が、小麦粉であることを特徴とする、請求項15に記載のサワードウの製造方法。
- ステップi)において、前記得られたパン生地が、150〜160のパン生地の収量(DY)を有することを特徴とする、請求項14に記載のサワードウの製造方法。
- 前記発酵が、28〜35時間行われることを特徴とする、請求項14に記載のサワードウの製造方法。
- 前記発酵が、75〜80%の相対湿度で行われることを特徴とする、請求項14に記載のサワードウの製造方法。
- 前記発酵が、35℃の温度及び76%の相対湿度で31時間行われることを特徴とする、請求項14に記載のサワードウの製造方法。
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JP6812092B2 (ja) * | 2015-05-20 | 2021-01-13 | 株式会社カネカ | プロテアーゼを含有する菓子及び菓子用生地 |
KR101551836B1 (ko) * | 2015-05-22 | 2015-09-09 | 에스피씨 주식회사 | 한국 전통 누룩으로부터 분리한 제빵용 신규의 토종 천연 유산균 |
CN105941526A (zh) * | 2016-06-22 | 2016-09-21 | 蚌埠市老顽童食品厂 | 一种健康烘焙糕点发泡剂 |
EP3653058B1 (en) * | 2017-07-12 | 2022-09-07 | González de La Torre, Javier | Method for degradation of gliadin to obtain gluten-free flour |
EP3849325A1 (de) * | 2018-09-14 | 2021-07-21 | DIOSNA Dierks & Söhne GmbH | Verfahren zur herstellung von brotteig, brotteig und starter |
CN113456542A (zh) * | 2020-03-30 | 2021-10-01 | 仙婷(广州)科技研发有限公司 | 一种组合物及其应用 |
CN111826324A (zh) * | 2020-08-05 | 2020-10-27 | 厦门惠盈动物科技有限公司 | 一种鼠李糖乳杆菌菌粉的制备方法 |
CN112056496A (zh) * | 2020-09-09 | 2020-12-11 | 中国农业大学 | 一种利用乳酸菌降低面团麸质蛋白含量的方法 |
DE102021130566A1 (de) | 2021-11-23 | 2023-05-25 | Lars Laumeyer | Verfahren und vorrichtung betreffend den erhalt einer sauerteig-kultur in der heimanwendung |
WO2024009249A1 (en) * | 2022-07-07 | 2024-01-11 | Il Granaio Delle Idee S.R.L. | Processing aid comprising encapsulated sourdough lactic acid bacteria for the preparation of doughs for bakery products |
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ITRM20060369A1 (it) * | 2006-07-17 | 2008-01-18 | Giuliani Spa | Miscela di batteri lattici per la preparazione di prodotti da forno senza glutine |
US20080299258A1 (en) * | 2007-04-18 | 2008-12-04 | Roman Stephen B | Probiotic natural sweetener compositions with standardized levels of fiber and process to make |
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US9974315B2 (en) | 2018-05-22 |
PT2818048T (pt) | 2019-05-08 |
MX2014008890A (es) | 2014-09-25 |
CN104780765A (zh) | 2015-07-15 |
CN104780765B (zh) | 2018-10-09 |
RU2628314C2 (ru) | 2017-08-15 |
BR112015010052A2 (pt) | 2017-07-11 |
UA116550C2 (uk) | 2018-04-10 |
AU2012394164A1 (en) | 2015-05-21 |
CA2864814A1 (en) | 2014-05-15 |
WO2014072758A1 (es) | 2014-05-15 |
EP2818048A1 (en) | 2014-12-31 |
EP2818048B1 (en) | 2019-01-09 |
HRP20190663T1 (hr) | 2019-05-31 |
ES2719686T3 (es) | 2019-07-12 |
IL238652A0 (en) | 2015-06-30 |
RU2015121707A (ru) | 2016-12-27 |
CR20150283A (es) | 2015-08-10 |
EP2818048A4 (en) | 2015-11-04 |
IN2015DN03789A (ja) | 2015-10-02 |
JP2015533290A (ja) | 2015-11-24 |
US20150140167A1 (en) | 2015-05-21 |
NI201500065A (es) | 2015-09-18 |
AR093344A1 (es) | 2015-06-03 |
IL238652B (en) | 2019-05-30 |
PL2818048T3 (pl) | 2019-08-30 |
DK2818048T3 (en) | 2019-04-23 |
BR112015010052B1 (pt) | 2020-03-17 |
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