JP6270986B2 - 単一ヌクレオチド検出方法 - Google Patents

単一ヌクレオチド検出方法 Download PDF

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Publication number
JP6270986B2
JP6270986B2 JP2016507056A JP2016507056A JP6270986B2 JP 6270986 B2 JP6270986 B2 JP 6270986B2 JP 2016507056 A JP2016507056 A JP 2016507056A JP 2016507056 A JP2016507056 A JP 2016507056A JP 6270986 B2 JP6270986 B2 JP 6270986B2
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oligonucleotide
nucleotide
stranded
region
different
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Japanese (ja)
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JP2016515832A (ja
Inventor
アレキサンダー フレイリング キャメロン
アレキサンダー フレイリング キャメロン
バームフォース バーナビー
バームフォース バーナビー
フラヴィオ ノゲイラ デ スーザ ソアレス ブルーノ
フラヴィオ ノゲイラ デ スーザ ソアレス ブルーノ
ヘンリー アイザック トーマス
ヘンリー アイザック トーマス
ブレイナー ボリス
ブレイナー ボリス
ナターレ アレッサンドラ
ナターレ アレッサンドラ
アマーシオ ミシェル
アマーシオ ミシェル
ディア ポール
ディア ポール
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Medical Research Council
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Medical Research Council
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP2016507056A 2013-04-09 2014-04-09 単一ヌクレオチド検出方法 Expired - Fee Related JP6270986B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201306444A GB201306444D0 (en) 2013-04-09 2013-04-09 Single nucleotide detection method
GB1306444.9 2013-04-09
PCT/GB2014/051105 WO2014167323A1 (en) 2013-04-09 2014-04-09 Single nucleotide detection method

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016253797A Division JP6343659B2 (ja) 2013-04-09 2016-12-27 マイクロ流体核酸配列決定装置及びその使用

Publications (2)

Publication Number Publication Date
JP2016515832A JP2016515832A (ja) 2016-06-02
JP6270986B2 true JP6270986B2 (ja) 2018-01-31

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JP2016507056A Expired - Fee Related JP6270986B2 (ja) 2013-04-09 2014-04-09 単一ヌクレオチド検出方法
JP2016253797A Active JP6343659B2 (ja) 2013-04-09 2016-12-27 マイクロ流体核酸配列決定装置及びその使用

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JP2016253797A Active JP6343659B2 (ja) 2013-04-09 2016-12-27 マイクロ流体核酸配列決定装置及びその使用

Country Status (12)

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US (3) US9828631B2 (ko)
EP (2) EP2857524B1 (ko)
JP (2) JP6270986B2 (ko)
KR (1) KR101876189B1 (ko)
CN (2) CN105143468B (ko)
AU (2) AU2014252804B2 (ko)
BR (1) BR112015025762A2 (ko)
CA (1) CA2907865C (ko)
DK (2) DK2828408T3 (ko)
ES (2) ES2558610T3 (ko)
GB (1) GB201306444D0 (ko)
WO (1) WO2014167323A1 (ko)

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GB201310584D0 (en) * 2013-06-13 2013-07-31 Base4 Innovation Ltd Droplet storage method
GB201402644D0 (en) * 2014-02-14 2014-04-02 Base4 Innovation Ltd Methylation detection method
GB201412977D0 (en) * 2014-07-22 2014-09-03 Base4 Innovation Ltd Single nucleotide detection method
GB201501012D0 (en) * 2015-01-21 2015-03-04 Base4 Innovation Ltd Improved droplet sequencing apparatus and method
EP3115109A1 (en) 2015-07-06 2017-01-11 Base4 Innovation Limited Droplet sequencing device
EP3207982A1 (en) 2016-02-17 2017-08-23 Base4 Innovation Limited Improved droplet sequencing apparatus and method
EP3211092B1 (en) 2016-02-24 2019-03-27 Base4 Innovation Ltd Single nucleotide detection method
EP3269444A1 (en) 2016-07-14 2018-01-17 Base4 Innovation Ltd Method of identifying droplets in a stack and an associated sequencer
WO2018042028A1 (en) 2016-09-02 2018-03-08 Base4 Innovation Limited Single nucleotide detection method and associated probes
CN109790573A (zh) * 2016-09-06 2019-05-21 贝斯4创新公司 单核苷酸检测方法及相关的探针
JP2019532632A (ja) 2016-09-20 2019-11-14 ベース４ イノベーション リミテッド 単一ヌクレオチド検出方法および関連プローブ
WO2018210823A1 (en) 2017-05-15 2018-11-22 Base4 Innovation Limited Single nucleotide detection method and associated probes
CN110831697B (zh) 2017-06-21 2022-03-15 光投发现有限公司 微滴操控设备和使用该设备操控水性微滴的方法
BR112019027764A2 (pt) * 2017-06-21 2020-07-07 Base4 Innovation Limited método para manipular uma microgotícula e método para pirofosforizar um analito de ácido nucleico
EP3641935B1 (en) 2017-06-21 2021-09-08 Lightcast Discovery Ltd Device for investigating nucleic acids
CN111263818A (zh) * 2017-10-23 2020-06-09 贝斯4创新公司 单核苷酸分析方法及相关探针
US20210213453A1 (en) 2018-05-18 2021-07-15 Lightcast Discovery Ltd. Droplet manipulation device and method
WO2019243577A1 (en) 2018-06-21 2019-12-26 Base4 Innovation Limited Sequencing method using modified nucleoside polyphosphates
PL3693086T3 (pl) 2019-02-08 2021-04-19 Lightcast Discovery Ltd Sposób manipulacji mikrokroplami
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
IL293936A (en) * 2019-12-23 2022-08-01 Biofidelity Ltd kits and devices

Family Cites Families (19)

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JP2575270B2 (ja) 1992-11-10 1997-01-22 浜松ホトニクス株式会社 核酸の塩基配列決定方法、単一分子検出方法、その装置及び試料の作成方法
EP0682671A4 (en) 1993-02-01 1998-01-14 Seq Ltd METHOD AND DEVICES FOR SEQUENCING DNA.
US6268146B1 (en) * 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
WO2000071755A2 (en) * 1999-05-25 2000-11-30 Praelux Incorporated Method for sequency and characterizing polymeric biomolecules using aptamers and a method for producing aptamers
GB9923644D0 (en) * 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
EP1448798A1 (de) * 2001-11-27 2004-08-25 Gnothis Holding SA Nanostruktur insbesondere zur analyse von einzelmolekülen
WO2003080861A1 (en) * 2002-03-22 2003-10-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Single molecule sequencing using phosphate labeled nucleotides
US7476501B2 (en) 2002-03-26 2009-01-13 Intel Corporation Methods and device for DNA sequencing using surface enhanced raman scattering (SERS)
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US20070015176A1 (en) * 2005-02-18 2007-01-18 Applera Corporation Small nucleic acid detection probes and uses thereof
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Also Published As

Publication number Publication date
DK2857524T3 (en) 2017-05-08
US20180044729A1 (en) 2018-02-15
US20160040224A1 (en) 2016-02-11
JP2016515832A (ja) 2016-06-02
KR101876189B1 (ko) 2018-07-09
JP2017079781A (ja) 2017-05-18
AU2016259441A1 (en) 2016-12-08
GB201306444D0 (en) 2013-05-22
JP6343659B2 (ja) 2018-06-13
EP2828408A1 (en) 2015-01-28
EP2828408B1 (en) 2015-10-14
ES2620982T3 (es) 2017-06-30
US20180044728A1 (en) 2018-02-15
BR112015025762A2 (pt) 2017-10-10
US10690689B2 (en) 2020-06-23
CA2907865A1 (en) 2014-10-16
EP2857524B1 (en) 2017-01-25
US9828631B2 (en) 2017-11-28
CN105143468B (zh) 2017-07-07
CA2907865C (en) 2018-02-27
CN105143468A (zh) 2015-12-09
AU2014252804A1 (en) 2015-10-15
ES2558610T3 (es) 2016-02-05
WO2014167323A1 (en) 2014-10-16
AU2016259441B2 (en) 2017-03-16
DK2828408T3 (en) 2015-12-14
CN107090492A (zh) 2017-08-25
KR20150139959A (ko) 2015-12-14
US10551399B2 (en) 2020-02-04
EP2857524A1 (en) 2015-04-08
AU2014252804B2 (en) 2017-01-05

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