JP6204174B2 - Pet food - Google Patents

Description

  The present invention relates to pet foods such as dogs and cats bred as pets (companion animals), and particularly to pet foods effective for improving the intestinal environment of pets.

  Due to the recent pet boom, the number of pets has increased and pet obesity has increased due to lack of exercise, overnutrition, etc., and attention has been focused on pet diet and health management. For example, Patent Document 1 discloses that the pet food for dogs and cats contains a short-chain oligofructose containing 1-kestose, nystose and 1F-β-fructofuranosyl nystose, whereby the gastrointestinal tract of dogs and cats. It is described that the health and manure odors are improved. Patent Document 2 describes that the inclusion of plum extract in pet food for dogs and cats eliminates the urine and body odors of dogs and cats and reduces the frequency of causing diarrhea symptoms. As plum extract, it is also described that plum juice obtained by squeezing raw plum or concentrate thereof, plum vinegar after salting plum or processed product thereof, and the like. Patent Document 3 discloses pet foods for dogs and cats, and extracts of rosemary, sunflower seeds, fresh coffee beans, grape skins, grape seeds, apples, carrot leaves, and tea leaves. It is described that the fecal urine odor of dogs and cats is reduced or deodorized by containing a compounding agent containing at least one extract selected from the group consisting of and an enzyme that oxidizes phenolic compounds. ing.

  Further, Patent Document 4 describes that an antiallergic agent containing a lactic acid bacterium belonging to Leuconostoc mesenteroides isolated from iron gun soaking has a high IgE antibody production inhibitory effect. An antiallergic agent containing and having an antiallergic action has been proposed. Patent Document 4 also describes that this lactic acid bacterium is the lactic acid bacterium Leuconostoc mesenteroides RIE strain deposited under the accession number FERM P-21110.

JP 2013-126416 A JP 2005-278404 A JP 2000-50814 A JP 2008-231094 A

  The subject of this invention is related to the pet food which can improve the intestinal environment of a pet and can suppress the malodor resulting from feces.

  The present invention is a pet food containing lactic acid bacteria belonging to Leuconostoc mesenteroides.

  ADVANTAGE OF THE INVENTION According to this invention, the pet food which can improve the intestinal environment of a pet and can suppress the malodor resulting from feces is provided.

  The pet food of the present invention contains lactic acid bacteria belonging to Leuconostoc mesenteroides. As this lactic acid bacterium, Leuconostoc mesenteroides RIE strain is preferably used. The lactic acid bacterium Leuconostoc mesenteroides RIE strain has been deposited at the National Institute of Advanced Industrial Science and Technology and the Patent Organism Depositary, and the deposit number is FERM P-21110. Since the lactic acid bacterium Leuconostoc mesenteroides RIE is isolated from a material with abundant food experience (steel pickled), it can be safely used for pet food.

  Details of the mycological properties and the like of the lactic acid bacterium Leuconostoc mesenteroides RIE are described in Patent Document 4. Regarding the lactic acid bacterium Leuconostoc mesenteroides RIE strain, Patent Document 4 describes that the lactic acid bacterium Leuconostoc mesenteroides RIE strain has a high IgE antibody production inhibitory effect, and human hay fever symptoms (sneeze attack, nasal discharge) In patent document 4, the lactic acid bacterium Leuconostoc mesenteroides RIE strain is effective in improving the intestinal environment of dogs and cats (reducing the fecal odor of dogs and cats). It is not described to have. It has been found for the first time by the present inventors that the lactic acid bacterium Leuconostoc mesenteroides RIE strain has an effect of suppressing malodor caused by feces of pets such as dogs and cats.

  The lactic acid bacterium Leuconostoc mesenteroides RIE can be prepared by culturing under suitable conditions using a medium usually used for culturing microorganisms. After culturing, the resulting lactic acid bacterium Leuconostoc mesenteroides RIE may be used as it is, and if necessary, crude purification by centrifugation, etc .; solid-liquid separation by filtration, etc .; filtration sterilization, radioactive sterilization, superheated type You may perform well-known sterilization processes, such as sterilization and pressurization-type sterilization. Further, the lactic acid bacterium Leuconostoc mesenteroides RIE strain can be dried to obtain a powdery product or a granular product. Although it does not restrict | limit especially as a specific drying method, For example, spray drying, spray cool, drum drying, vacuum drying, freeze drying etc. are mentioned, These can be employ | adopted individually or in combination. In that case, you may add the excipient | filler normally used as needed.

  The content of lactic acid bacteria belonging to Leuconostoc mesenteroides (Lactobacillus leuconostoc mesenteroides RIE strain) in the pet food of the present invention is an amount that can improve the intestinal environment of pets such as dogs and cats, more specifically May be an amount that can reduce fecal odor (especially indole and p-cresol) of pets, and can be appropriately adjusted according to the form of pet food, the type of pet to be applied, and the like. The content of the powdered or granular material of the lactic acid bacteria is preferably 0.0001 to 5% by mass, more preferably 0.001 to 1% by mass, based on the total mass of the pet food in the pet food of the present invention. It is.

  The pet food of the present invention usually contains basic raw materials in addition to the lactic acid bacteria. As this basic raw material, those conventionally used as raw materials in this kind of pet food can be used as appropriate, for example, meat flour, fish flour, cereals (wheat flour, corn flour, soybean flour, rice flour, various starches). Thickeners such as soybean meal, rice bran, rice bran, bran, germ, malt, okara, wheat gluten, fats and oils, vitamins, minerals, egg products, gums, gelling agents, salt, seasoning Examples of these materials include spices and spices, and these can be used alone or in combination of two or more.

  The pet food of the present invention is not limited as long as it can retain its solid shape without causing major deformation or collapse during handling such as distribution, storage, sales, and feeding to pets. In general, pet food is largely classified into a moist type with a moisture content of usually 50% by mass or more, a semi-moist type with a moisture content of usually about 20 to 40% by weight, and a dry type with a moisture content of usually about 10% by weight or less. Apart from that, the pet food of the present invention includes at least dry type and semi-moist type pet foods. Among them, the dry type is preferable because it is excellent in handling properties, ease of production, storage stability, and the like.

  The shape of the pet food of the present invention is not particularly limited, and can be the same shape as the conventional dry type, semi-moist type pet food, for example, pellet shape, granular shape, stick shape, donut shape, star shape, It can be formed in a desired shape such as a dog-bone shape, a ball shape, a flat round shape, a spherical shape, an elliptical shape, or a rectangular shape. Further, the size of the pet food of the present invention is not particularly limited, and can be set according to the type and age of the pet to be fed. For example, in a pellet form, when the diameter is about 5 to 10 mm and the length is about 10 to 15 mm, feeding to a dog or cat is easy and the palatability is excellent.

The pet food of this invention can be manufactured according to the manufacturing method of the conventional pet food. The following manufacturing method AC is mentioned as an example of the manufacturing method of the pet food of this invention. In the following production methods A to C, the raw material or the pulverized product thereof may be formed using a method conventionally used for forming (granulating) a powder raw material in the production of this kind of solid pet food. it can.
(A) A method of producing a molded product not containing the lactic acid bacteria using a basic raw material or a pulverized product thereof, and imparting the lactic acid bacteria to the surface of the molded product.
(B) A method in which the lactic acid bacterium is added to the ground material of the basic material to obtain a mixture, and the mixture is molded according to a conventional method.
(C) A method in which the lactic acid bacteria are added to the basic raw material before pulverization to obtain a mixture, the mixture is pulverized, and the pulverized product is molded according to a conventional method.

  Among them, the pet food obtained by the manufacturing method A, that is, “the pet food comprising the inner layer part not containing the lactic acid bacteria and the surface layer part covering the surface of the inner layer part and containing the lactic acid bacteria” ( The pet food is a solid pet food in which the lactic acid bacteria are unevenly distributed on the surface layer), and is more effective than the pet food obtained by other manufacturing methods (pet food in which the lactic acid bacteria are uniformly dispersed). Therefore, it is preferably used in the present invention. In the production method A, as a method for imparting the lactic acid bacterium to the surface of the molded body, for example, the powdered lactic acid bacterium is used, and the lactic acid bacterium alone or a mixture of the lactic acid bacterium and a palatability material is formed by a technique such as dusting. Examples include a method of coating the body. As means for fixing the lactic acid bacteria to the surface of the molded body, for example, fats and oils can be used. The coating amount of the lactic acid bacteria is preferably in the range of 0.0001 to 5 mass%, more preferably 0.001 to 1 mass%, based on the total mass of the molded body.

  The pet food of the present invention is suitable as a food for small animals that can be raised at home, such as dogs, cats, rabbits, and nine-bird birds. The pet food of the present invention is particularly suitable as a dog food or a cat food, particularly a cat food, as illustrated in the examples described later, and improves the intestinal environment of the cat, effectively preventing malodor caused by feces inherent to the cat. Can be suppressed.

  Examples will be given to specifically describe the present invention, but the present invention is not limited to the examples.

[Example 1]
Prepare the materials shown in Table 1 below as basic raw materials, and use an extruder ("EP-50" manufactured by Joda Iron Works Co., Ltd.) to supply the raw materials to the extruder at 60kg / hour and supply water to the extruder. Under the conditions of 13 liters / hour, kneading temperature 70 ° C. to 90 ° C., barrel tip temperature 100 ° C. to 130 ° C., barrel tip pressure 5 to 10 bar, the basic material is extruded and extruded into strands, and then cut. Then, it was dried at 120 ° C. to produce a pellet-shaped molded body having a moisture content of 8%. This molded body had a diameter of 8-9 mm and a length of 3-5 mm. Then, the entire surface of the molded body is coated with a fat and oil that is solid at room temperature, and further, a mixture of the powdered material of the lactic acid bacterium Leuconostoc Mesenteroides RIE and the palatable material is coated on the entire surface of the molded body. The target dry type cat food was manufactured. In the obtained cat food, the content of the powdered product of the lactic acid bacterium Leuconostoc mesenteroides RIE was 0.01% by mass with respect to the total mass of the cat food.

[Comparative Example 1]
A dry-type cat food was produced in the same manner as in Example 1 except that the lactic acid bacterium Leuconostoc mesenteroides RIE was not used. The pet food of Comparative Example 1 is the molded body itself.

[Cat food evaluation test]
Five cats (Japanese cat and Scottish fold, average body weight 3.1 kg) having an average age of 6 years old were fed cat food for 8 weeks and allowed to eat freely. Of the 8-week test period, only the cat food of Comparative Example 1 was fed for the first 4 weeks (control group), and only the cat food of Example 1 was fed for the remaining 4 weeks (test group). During the test period, the cat food was maintained at 16:00 in the evening every day (70 × kg body weight), and the uneaten cat food was collected at 9 am the next morning. During the test period, water was always supplied. In each of the control group and the test group, feces were collected on the 23rd and 24th days from the start of feeding cat food. In each of the control group and the test group, feces excreted from 9:00 am to 17:00 in the evening were collected, and the amount of bacterial metabolites of the test specimen cat was evaluated by the following method.

<Amount of bacterial metabolites>
The contents of indole and p-cresol in dry feces were measured. These substances are the main malodor components of feces, and the lower the content of these in dry feces, the lower the malodor caused by feces. The content of indole and p-cresol was measured as follows. 1.0 g of feces is precisely weighed in a 50 ml glass test tube, 5 ml of 1.0 M phosphate buffer (pH 6.0) is added and suspended, and 100 μl of 0.04% p-isopropylphenol is added as an internal standard. 5 ml of carbon sulfide was added, and extraction was performed at 0 ° C. for 30 minutes with a shaking incubator. After centrifuging at 3000 rpm for 15 minutes, 4 μl of the lower layer of the extract was taken in a microsyringe and analyzed by GC (GC-2014: Shimadzu Corporation).

  As is clear from the results shown in Table 2, in Example 1 (test group), the fecal odor reduction effect superior to that of Comparative Example 1 (control group) (indole, which is a malodorous component among bacterial metabolite amounts) p-cresol decrease) was observed.

Claims (1)

A pet food for reducing fecal odor of cats containing lactic acid bacteria belonging to Leuconostoc mesenteroides ,
An inner layer part that does not contain the lactic acid bacteria, and a surface layer part that covers the surface of the inner layer part and contains the lactic acid bacteria in a powdery or granular form,
The lactic acid bacterium is Leuconostoc mesenteroides RIE strain (Accession No. FERM P-21110);
A pet food having a powdery or granular content of the lactic acid bacteria of 0.0001 to 5 mass% .