KR102136559B1 - Animal Food Composition and Feed Additive comprising Kefir - Google Patents

Abstract

The present invention relates to a food composition and feed additive for a companion animal including kefir, and has a reduction effect of indicator microorganisms in the intestine related to constipation of a companion animal due to the probiotic effect of kefir, and has an effect of improving occult blood , It can be useful for food such as snacks of companion animals.

Description

Animal food composition and feed additive comprising kefir

The present invention relates to a food composition and feed additives for companion animals comprising kefir.

Currently, in Korea, households with 1-2 people who are beyond nuclear family are being made, and the population of pets is increasing. The largest portion of the companion animal is the companion dog, and it is generally known that the companion dog lives on average for about 11 to 13 years. Recently, the life span has been extended to about 15 to 20 years due to public health improvement and medical improvement. In addition, because of the increased level of national consciousness, companion animals are now often considered human-like personalities.

However, most of the dogs in Korea live in a house, and they tend to be exposed to metabolic diseases easily because of lack of exercise and stress. The most representative is dog obesity, and about 30-53% of domestic dogs are reported to be overweight or obese. When a dog has metabolic obesity, similar to humans, it can cause more fatal internal and external diseases such as cardiovascular disease, joint disease, and immune disease, which can significantly degrade the quality of life of the dog and dog.

On the other hand, the interest in animal health in Korea is currently increasing, but due to the high cost of medical treatment due to the high cost of realistic treatment, the development of an agent that can easily prevent the occurrence and progression of diseases such as constipation or obesity due to the lack of exercise of companion dogs It is an urgent situation.

Kefir is a natural fermented milk in which more than 50 types of lactic acid bacteria, acetic acid bacteria, and yeast form a symbiotic system. Kefir is a natural fermented milk made by fermenting animal milk using a special strain called kefir grain, and there has been no precedent for using kefir as a dog snack.

Accordingly, the present inventors completed the present invention by confirming the palatability and health-benefit effect of the composition including kefir on the companion animal.

1. Republic of Korea Patent Registration No. 10-1426399

One object of the present invention is to provide a food composition for preventing or improving constipation and constipation in a companion animal, wherein the companion animal is a dog. Is to do.

Another object of the present invention is to provide a method of treating occult blood and constipation in a dog, comprising ingesting the composition into a dog.

Another object of the present invention is to provide a feed additive for preventing or improving constipation and constipation in a companion animal, which is a feed for ingestion of dogs, in the feed additive for preventing or improving constipation comprising kefir. .

An aspect of the present invention provides a food composition for preventing or improving constipation and constipation in a companion animal, wherein the companion animal is a dog. .

Kefir contained in the food composition of the present invention, while improving the palatability of food for companion animals, it is possible to prevent excessive intake of food, to improve the symptoms of constipation by softening the stool, as well as useful intestinal flora It can be effectively used to prevent or improve constipation in companion animals.

As used in the present invention, the term "prevention" means that the normal weight or normal bowel movement state of the companion animal is maintained.

In the present invention, the term, "improvement" refers to an increase in the content of water contained in the weight loss or feces of a companion animal belonging to the category of obesity, and includes improving or beneficially altering symptoms due to occult blood and constipation.

The food composition of the present invention may further include a suitable carrier, excipient, and diluent commonly used in food production. Carriers, excipients and diluents include, for example, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, It can be methyl cellulose, microcrystalline cellulose, polyvinyl tolidon, water, honey, methylhydroxybenzoate, propylhydroxy benzoate, magnesium stearate and mineral oil. When formulated, it may be prepared using diluents or excipients such as fillers, weights, binders, wetting agents, disintegrating agents, surfactants, etc., which are usually used. When prepared as a solid preparation, the composition may include at least one excipient, for example, starch, calcium carbonate, sucrose or lactose, gelatin, and the like. In addition to the commonly used simple diluents, water and liquid paraffin, various excipients may be included, such as wetting agents, sweeteners, fragrances and preservatives.

Further, the food composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and fillers (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and the like. It may contain salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonic acid used in carbonated beverages, and the like. In addition, it may contain flesh or vegetables for the production of natural beverages, and these ingredients may be used independently or in combination.

The food composition of the present invention may be used as kefir itself or may be used in an amount of 0.01 to 99.99% by weight of the food composition.

The food composition of the present invention may further include a known substance exhibiting activity in the symptoms of occult blood or constipation in a companion animal.

The food composition of the present invention can be used alone or in combination with methods using surgery, hormonal therapy, drug therapy, and/or biological response modifiers to improve the symptoms of occult blood or constipation in a companion animal.

According to one embodiment of the invention, the kefir may be administered orally at 160 to 200ml/day.

According to one embodiment of the invention, the kefir may be to increase the growth of Lactobacillaceae and Prevotella copri bacteria in the gut of the dog.

Another aspect of the present invention provides a method for treating canine congestion and constipation in a dog comprising ingesting the composition into a dog.

The term "treatment" used in the present invention means that the symptoms of occult blood or constipation in a companion animal are improved or advantageously changed.

The provided amount of the food composition of the present invention can be provided so that the companion animal can ingest freely, but may vary depending on the weight of the companion animal, and the amount of kefir included in the food composition of the present invention is the weight of the companion animal (kg ) May be 2 ml to 20 ml per sugar, preferably 5 ml to 10 ml

In another aspect of the present invention, in the feed additive for preventing or improving constipation, including kefir, the feed provides a feed additive for preventing or improving constipation and constipation of a companion animal, which is a feed for dog intake.

As used in the present invention, the term "feed additive" refers to a substance that is added to the feed to improve the productivity of livestock, improve and/or maintain the health of companion animals, or maintain physical condition. In the present invention, kefir may be included as an active ingredient.

The content of the feed additive included in the feed is not particularly limited, but may be 0.01 to 20.0% (w/w), and preferably 0.05 to 10.0% (w/w).

The feed additive of the present invention is not limited to this, but animals such as cows, pigs, sheep, goats, and deer are divided into two hoofs (predators), birds such as chickens, ducks, geese, pheasants, eel, flounder fish, dogs, cats, etc. It can be added to any one or more of the pets such as pets.

The feed additive of the present invention may further include an excipient. The excipients of the present invention refer to components added for the purpose of giving a suitable hardness or shape to the active ingredient, or in the case of a small amount of main material, to give a certain capacity and weight to be easy to handle. The excipient may include those commonly used in the manufacture of feed additives, but is not limited thereto, and may include any one or more of wheat bran, corn powder, cereal starch, silica powder, diatomaceous earth, and feed standards. And excipients recognized in the standard (Ministry of Agriculture, Food and Rural Affairs, Announcement No. 2014-106).

The feed additive of the present invention may be prepared in a conventional formulation in the art, and may be embodied as a powder or a pellet shape having a predetermined shape, but is not limited thereto. The feed additive may be powdered to enhance absorption in the body, or may be prepared in a fat coating formulation to enhance antibacterial activity in the small intestine.

The excipient may be included in 50 to 99 parts by weight based on 100 parts by weight of the total feed additive.

The companion animal food composition of the present invention has an effect of reducing indicator microorganisms in the intestine related to constipation of the companion animal by the probiotic effect of kefir, and has an effect of improving occult blood, so it is usefully utilized for foods such as snacks of companion animals. Can be.

1 is a graph showing changes in the intestinal microbial indicator Firmicutes/Bacteroidetes ratio in an animal model.
2 is a graph showing changes in Lactobacillaceae, a microorganism associated with systemic inflammation and obesity, in an animal model.
3 is a graph showing changes in Prevotella copri that compete with obesity-causing microorganisms in an animal model.
4 is a graph showing changes in Faecalibacterium prausnitzii, a microorganism related to obesity, in an animal model.

Hereinafter, one or more specific examples will be described in more detail through examples. However, these examples are intended to illustrate one or more embodiments by way of example, and the scope of the present invention is not limited to these examples.

Example 1. Preparation of kefir

Kefir was prepared and supplied as follows daily during the experiment period.

50 g of Kefir grain (Sensorgen Co., Seoul, Korea) was inoculated into 500 ml of sterile milk (Seoul Milk, Inc., Seoul, Korea) and cultured at 25° C. for 24 hours. After incubation, kefir was prepared by separating kefir grain and kefir using a sterile plastic filter (2 mm pore size).

Example 2. Animal model preparation and evaluation method

<2-1> Animal model experimental group design

Healthy adult dogs (n=6, 4 males, 2 females) with an age of 3 to 8 years (average age 5.17 ± 2.32) and a weight of 24.2 to 32.0 kg (average BW 28.65 ± 2.94 kg) were used. The dogs used in the experiment were the breeds of Mongrel, Poodle, Marinois, Rabrado and Shepherd. All experimental procedures were approved and carried out in accordance with the guidelines of Seojeong University Animal Experimental Ethics Committee (SJ2017-04). Dogs were kept for 14 days in individual cages with fresh water and feed and exercise. As the feed, commercially available pellet-type dog food (Natural balance Ultra Premium (California Burbank) and Natural Balance Pet Foods® (Dick Van Patten)) was supplied according to body weight. All other snacks were not provided except feed, and the data before and after intake (day 0) and after intake (day 14) of kefir were compared. During the experiment period, 200 ml of kefir was provided for autonomous intake once a day.

<2-2> Confirmation of changes in body condition and eating habits according to kefir intake

The animal models on day 0 and day 14 of the experiment were analyzed for changes in body condition scores and eating habits.

Specifically, during the intake period of kefir, the intake of kefir as a snack through the indicators of kefir intake compared to kefir intake, time taken for kefir intake, and feed intake and negative volume, affects the physical condition and general eating habits of the animal model. Was confirmed.

<2-3> Fecal quality inspection

The fecal quality changes of animal models on day 0 and day 14 of the experiment were analyzed.

Specifically, fecal samples were scored according to Bristol's scoring guidelines as shown in Table 1 below.

score shape One cake 2 Lumpy sausage form 3 Sausage with cracks on the surface 4 Soft and tender sausage or snake form 5 Edge of clean edge 6 Edge with irregular edges 7 Liquid stool

Fecal water content was measured by taking 1 g of fecal sample. The fecal water content was dried in a drying oven at 80° C. for 24 hours, and then calculated according to the following formula.

100 × (fecal weight before drying-fecal weight after drying) / fecal weight before drying

On the other hand, according to the manufacturer's instructions, Hema-Screen Lab Pack (Stanbio Laboratory, Boerne, TX, USA) was used to perform occult blood test in feces.

<2-4> Analysis of intestinal microflora

The intestinal microbial changes of the animal models on day 0 and day 14 of the experiment were analyzed.

Specifically, DNA was extracted from the fecal sample according to the manufacturer's instructions using NucliSENS easyMAG equipment (bioMerieux, Marcy l'Etoile, France). Then, using the extracted DNA, the intestinal microflora group associated with obesity was quantified using qPCR analysis.

Meanwhile, the intestinal microflora was also analyzed through next generation sequencing. After preparing each sequencing sample according to the Illumina 16S Metagenomic Sequencing Library protocol, the quality was screened with PicoGreen and Nanodrop, and finally, after amplifying the V3-V4 region of the 16S rRNA gene, analysis was performed.

<2-5> Statistical Analysis

All data are expressed as mean±standard deviation (SD). Statistical analysis was performed using SPSS Version 18.0 software (SPSS, Inc., Chicago, IL, USA). All data obtained from day 0 and day 14 samples were analyzed using paired t-test, and were considered statistically significant when P <0.05.

Example 3. Confirmation of maintenance of the physical condition of the animal model

Whether the body condition of the animal model was changed before and after the experiment was confirmed according to Example <2-2> above.

As a result, as shown in Table 2, the physical condition scores were not different before and after the experiment.

No. Bell age castle Body condition Day 0 Day 14 One Mongrel 4 Female 3 3 2 Poodle 8 Male 3 3 3 Shepherd 3 Female 3 3 4 Marinois 5 Male 3 3 5 Rabrado 8 Male 3 3 6 Shepherd 3 Male 3 3 Mean±SD - 5.17±2.32 - 3 3 p value - - - -

Example 4. Confirmation of animal model preference for kefir intake and maintenance of eating habits

Before and after the experiment, whether or not the kefir preference of the animal model and the food tube was changed was confirmed according to Example <2-2>.

As a result, the animal model ingested kefir autonomously when kefir was provided. Interestingly, kefir was consumed with great interest in kefir. In addition, most of the 200 ml kefir provided daily was consumed in a short time (Table 3), and some animal models were excited when trying to give kefir and showed a very desired behavior.

On the other hand, the animal model consumed almost all of the feed provided, and it was confirmed that the intake of kefir did not affect the feed feeding of the dog.

No. Feed
(g/day) Feed intake
(g/day) Kefir Supply
(Ml/day) Kefir intake
(Ml/day) Intake time
(minute) One 200 190 2.5 660 660 2 200 200 2.3 920 822.7 3 200 161 2.7 780 780 4 200 200 1.1 780 780 5 200 195 1.5 420 420 6 200 182.1 3.5 780 780 Mean±SD 200.00±0.00 188.02±14.86 2.27±0.86 723.33±169.90 707.12±150.96

Example 5. Confirmation of fecal quality change in animal model

Whether or not the fecal quality of the animal model before and after the experiment was confirmed according to Example <2-3> above.

As a result, in the quadrant after 14 days of kefir ingestion, although the Bristol score slightly increased visually, it did not show a significant difference. It was confirmed that the increase was increased ( p <0.05, Table 4), and also, no occult blood reaction was observed before and after the experiment.

No. Fecal water content (%) Bristol score Occult blood in feces Day 0 Day 14 Day 0 Day 14 Day 0 Day 14 One 62.06 72.68 4 4 N N 2 63.70 68.49 2 4 N N 3 54.35 72.91 4 4 N N 4 61.09 68.73 3 3 N N 5 68.07 70.29 4 4 N N 6 59.77 71.07 4 3 N N Mean±SD 62.51±4.14 70.70±1.73 3.5±0.84 3.67±0.52 0/6 0/6 p value 0.01 0.70 - -

That is, through the above results, it was confirmed that the intake of kefir tends to soften the feces of the dog, and does not cause inflammation or bleeding in the intestinal tract.

Example 6. Confirmation of changes in intestinal microflora of companion dogs according to kefir intake

Whether or not the intestinal microflora of the animal model before and after the experiment was confirmed according to Example <2-4> above.

As a result, after ingesting kefir, the intestinal microbial indicator Firmicutes/Bacteroidetes ratio, which is the closest to obesity, decreased (FIG. 1), and Lactobacillaceae, which is generally known to control systemic inflammation and obesity caused by pathogens by acidifying the intestinal environment It was confirmed that increases (Fig. 2). In addition, it was confirmed that Prevotella copri, which is known to compete in the gut with Bacteroides thetaiotaomicron, a representative obesity-causing bacterium, significantly increased (p<0.05, FIG. 3), and Faecalibacterium prausnitzii, which is known to increase in obese individuals, was significantly increased. The decrease was confirmed (p<0.05, FIG. 4).

That is, through the above results, kefir reduces the bacteria that can promote obesity among the bacteria present in the intestine of the dog, increases the bacteria that suppress obesity, prevents obesity when ingesting kefir for a long time Or it could be improved.

So far, the present invention has been focused on the preferred embodiments. Those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered in terms of explanation, not limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent range should be construed as being included in the present invention.

Claims (5)

In the food composition for preventing or improving occult blood and constipation of companion animals comprising kefir,
The companion animal is a dog,
The kefir is to increase the growth of Lactobacillaceae and Prevotella copri in the intestine of the dog.
Food composition for preventing or improving companion animal occult blood and constipation.
According to claim 1,
The kefir is a pet food composition for preventing or improving constipation and constipation of animals, which is administered orally at 160 to 200 ml/day.
delete Step of ingesting the composition of claim 1 to the dog (犬)
How to prevent or improve occult blood and constipation in a dog comprising a.
In the feed additive for preventing or improving constipation comprising kefir,
The feed is for dog intake
Feed additive for preventing or improving companion animals' occult blood and constipation.

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