KR20190122636A - Animal Food Composition and Feed Additive comprising Kefir - Google Patents

Abstract

The present invention relates to a pet food composition comprising a kefir and a feed additive, which has a reduction effect on the intestinal indicator microorganisms associated with constipation in pets due to a probiotic effect of the kefir, and improves occult blood, thereby being useful for foods such as snacks of pets.

Description

Animal food composition and feed additive comprising kefir {Animal Food Composition and Feed Additive comprising Kefir}

The present invention relates to a pet food composition and feed additive comprising a kefir.

At present, the number of households with one or two persons is growing beyond nuclear family, and the population of pets is increasing. The largest part of pets is dogs, which are generally known to live on average for 11 to 13 years, but have recently been extended to 15 to 20 years due to improved public health and medical care. In addition, pets are often considered to be similar to humans due to the increased level of public awareness.

However, most domestic dogs live in homes, so they lack exercise and are stressed and are easily exposed to metabolic diseases. The most representative is the obesity of dogs, and about 30-53% of domestic dogs are reported to be overweight or obese. If a dog suffers from metabolic obesity, it may cause more fatal internal and external medical diseases such as cardiovascular disease, joint disease, and immune disease, similar to humans, which may significantly reduce the quality of life of dogs and dogs.

On the other hand, the interest in veterinary medicine in Korea is increasing, but due to the high cost of medical treatment due to the high cost of treatment, the development of preparations that can easily prevent the development and progression of diseases such as constipation or obesity due to lack of exercise of dogs It is urgent.

Kefir is a natural fermented milk in which more than 50 kinds of lactic acid bacteria, acetic acid bacteria and yeast form a symbiotic system. Kefir is a natural fermented milk made by fermenting animal milk using a special seed called kefir grains. There is no precedent of using kefir as a snack for dogs.

Thus, the present inventors completed the present invention by confirming the palatability and health benefits of the composition containing the kefir on the companion animal.

1. Korea Patent Registration No. 10-1426399

One object of the present invention is to provide a food composition for preventing or improving companion animal occult blood and constipation containing kefir, wherein the companion animal is a dog (犬) to provide a food composition for preventing or improving companion animal occult blood and constipation. It is.

Another object of the present invention is to provide a method for treating canine occult blood and constipation comprising the step of ingesting the composition in a dog.

Still another object of the present invention is to provide a feed additive for constipation prevention or improvement comprising kefir, wherein the feed is a feed for dog consumption and constipation for preventing or improving constipation. .

One aspect of the present invention provides a food composition for preventing or improving companion animal occult blood and constipation containing kefir, wherein the companion animal is a dog. .

Kefir contained in the food composition of the present invention can improve the palatability of the food of the companion animal, can prevent excessive intake of food, can soften the stool to improve the symptoms of constipation, as well as the intestinal flora Therefore, it can be effectively used to prevent or improve the constipation of pets.

As used herein, the term "prevention" refers to maintaining the normal weight or normal bowel activity of the companion animal.

As used herein, the term "improvement" refers to an increase in the amount of water contained in the weight loss or feces of a companion animal belonging to the category of obesity, and includes improvements or beneficial changes due to occult blood and constipation.

The food composition of the present invention may be used further comprising suitable carriers, excipients and diluents commonly used in food preparation. Carriers, excipients and diluents are, for example, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, Methyl cellulose, microcrystalline cellulose, polyvinyl tolidone, water, honey, methylhydroxybenzoate, propylhydroxy benzoate, magnesium stearate and mineral oil. When formulated, it may be prepared using conventional diluents or excipients such as fillers, weights, binders, wetting agents, disintegrating agents, surfactants, and the like. When prepared as a solid preparation, the composition may include at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin and the like. In addition to the commonly used simple diluents, water and liquid paraffin, various excipients may be included, for example wetting agents, sweetening agents, fragrances, preservatives and the like.

In addition, the food composition of the present invention is a variety of nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition it may contain flesh or vegetables for the production of natural beverages, these components may be used independently or in combination.

The food composition of the present invention may be used as kefir itself or added in an amount of 0.01 to 99.99% by weight in the food composition.

The food composition of the present invention may further include a known substance that is active against the symptoms of occult blood or constipation in a companion animal.

The food composition of the present invention may be used alone or in combination with methods using surgery, hormonal therapy, drug therapy and / or biological response modifiers to ameliorate symptoms of occult blood or constipation in a companion animal.

According to one embodiment of the invention, the kefir may be administered orally at 160 to 200ml / day.

According to one embodiment of the invention, the kefir may be to increase the growth of Lactobacillaceae bacteria and Prevotella copri bacteria in the intestine of the dog.

Another aspect of the invention provides a method of treating canine occult blood and constipation comprising the step of ingesting said composition in a dog.

As used herein, the term "treatment" means that the symptoms of occult blood or constipation in a companion animal is improved or advantageously changed.

Provided amount of the food composition of the present invention may be provided so that the pet is free to eat, may vary depending on the weight of the pet, the amount of kefir contained in the food composition of the present invention is the weight of the pet (kg 2 mL to 20 mL), preferably 5 mL to 10 mL

Still another aspect of the present invention provides a feed additive for constipation prevention or improvement comprising kefir, wherein the feed is a feed for dog consumption and constipation for preventing or improving constipation.

As used herein, the term "feed additive" refers to a substance added to a feed for improving productivity of a livestock, promoting and / or maintaining the health of a companion animal, or maintaining physical condition. In the present invention, kefir may be included as an active ingredient.

The content of the feed additive included in the feed is not particularly limited, but may be 0.01 to 20.0% (w / w), preferably 0.05 to 10.0% (w / w).

The feed additive of the present invention is not limited thereto, but animals such as cows, pigs, sheeps, goats, deers, hooves, chickens, ducks, geese, pheasants, birds, eel, flatfish, fish, dogs and cats It may be added to the feed of any one or more of the companion animals.

Feed additives of the present invention may further comprise an excipient. The excipient of the present invention means a component added for the purpose of giving a suitable hardness and shape to the active ingredient, or for the purpose of giving a certain dose and weight to a size that is easy to handle when the amount of the main agent is small. The excipient may include those commonly used in the manufacture of feed additives, but is not limited thereto, and may include any one or more of bran, corn powder, cereal starch, silica powder, diatomaceous earth, feed, etc. And excipients recognized in the standard (Ministry of Agriculture, Food and Rural Affairs Notice 2014-106).

The feed additive of the present invention may be prepared in a conventional formulation in the art, but may be embodied in powder or pellet form having a predetermined form, but is not limited thereto. The feed additive may be powdered to enhance absorption in the body, or may be prepared in a fat coating formulation to enhance antibacterial activity in the small intestine.

The excipient may be included in an amount of 50 to 99 parts by weight based on 100 parts by weight of the total feed additive.

The pet food composition of the present invention has an effect of reducing the intestinal indicator microorganisms associated with the constipation of pets by the probiotic effect of kefir, and has an effect of improving occult blood, so it is useful for foods such as snacks of pets. Can be.

1 is a graph showing changes in the intestinal microbial index Firmicutes / Bacteroidetes ratio in the animal model.
2 is a graph showing changes in Lactobacillaceae, a microorganism associated with systemic inflammation and obesity, in an animal model.
3 is a graph showing the change of Prevotella copri to compete with the obesity-inducing microorganism in the animal model.
Figure 4 is a graph showing the change of Faecalibacterium prausnitzii, a microorganism associated with obesity, in an animal model.

Hereinafter, one or more embodiments will be described in more detail with reference to Examples. However, these examples are provided to illustrate one or more embodiments illustratively and the scope of the present invention is not limited to these examples.

Example 1 Preparation of Kefir

Kefir was prepared and fed as follows every day during the experiment.

50 g of kefir grain (Sensorgen Co., Seoul, Korea) was inoculated in 500 ml of sterile milk (Seoul Milk, Inc., Seoul, Korea) and incubated at 25 ° C. for 24 hours. After incubation, kefir was prepared by separating the kefir grains and kefir using a sterile plastic filter (2 mm pore size).

Example 2. Preparation and Evaluation Method of Animal Model

<2-1> Animal Model Experiment Group Design

Healthy dogs (n = 6, 4 males, 2 females) of age 3-8 years (mean age 5.17 ± 2.32) and body weight 24.2-32.0 kg (average BW 28.65 ± 2.94kg) were used. The dogs used in the experiments were Mongrel, Poodle, Marinois, Labrado and Shepherd breeds. All experimental procedures were approved and performed according to the guidelines of the Seojung University Animal Experimental Ethics Committee (SJ2017-04). Dogs were kept for 14 days in individual cages where fresh water and feed were provided and exercised. As a diet, commercially available pellet-type dog food (Natural balance Ultra Premium (Burbank, Calif.) And Natural Balance Pet Foods® (Dick Van Patten)) were fed at a weighted level. All other snacks were not provided except for the diet, and data were compared before and after kefir intake (day 0). A total of 200 ml of kefir was autonomously taken once a day for the duration of the experiment.

<2-2> Changes of physical condition and eating habits according to kefir intake

The physical condition scores and eating habits of animal models on day 0 and day 14 were analyzed.

Specifically, the provision of kefir as a snack affects the physical condition and general eating habits of the animal model through the indicators of kefir intake, kefir intake, feed intake and drinking water during the kefir intake period. It was confirmed that it was.

<2-3> Fecal Quality Inspection

Fecal quality changes of animal models on day 0 and day 14 were analyzed.

Specifically, fecal samples were scored as shown in Table 1 according to Bristol's scoring guidelines.

score shape One cake 2 Bumpy sausage form 3 Sausage form with cracks on the surface 4 Soft and tender sausage or snake form 5 Yanbian with clean edges 6 Yanbian with irregular edges 7 Liquid stools

1g of fecal samples were taken to measure fecal moisture content. Fecal moisture content was calculated after drying for 24 hours in a drying oven at 80 ℃.

 100 × (fecal weight before drying-fecal weight after drying) / fecal weight before drying

Meanwhile, fecal occult blood test was performed using a Hema-Screen Lab Pack (Stanbio Laboratory, Boerne, TX, USA) according to the manufacturer's instructions.

<2-4> Intestinal Microbiota Analysis

Intestinal microbial changes of animal models on day 0 and day 14 were analyzed.

Specifically, DNA was extracted from fecal samples using NucliSENS easyMAG equipment (bioMerieux, Marcy l 'Etoile, France) according to the manufacturer's instructions. Then, using the extracted DNA, the intestinal microbiota group related to obesity was quantified using qPCR analysis.

The intestinal microbiota was also analyzed through next generation sequencing. After sequencing samples were prepared according to the Illumina 16S Metagenomic Sequencing Library protocol, quality screening with PicoGreen and Nanodrop was performed to amplify the V3-V4 region of the 16S rRNA gene, followed by analysis.

<2-5> Statistical Analysis

All data are expressed as mean ± standard deviation (SD). Statistical analysis was performed using SPSS Version 18.0 software (SPSS, Inc., Chicago, IL, USA). All data from the 0 and 14 day samples were analyzed using a paired t-test and considered statistically significant when P <0.05.

Example 3. Confirmation of maintenance of body condition of the animal model

Whether the physical condition of the animal model was changed before and after the experiment was confirmed according to Example <2-2>.

As a result, the body condition score was not different before and after the experiment, as shown in Table 2 below.

No. Bell age castle Physical condition Day 0 Day 14 One Mongrel 4 Female 3 3 2 Poodle 8 Male 3 3 3 Shepherd 3 Female 3 3 4 Marinois 5 Male 3 3 5 Rabrado 8 Male 3 3 6 Shepherd 3 Male 3 3 Mean ± SD - 5.17 ± 2.32 - 3 3 p value - - - -

Example 4 Confirmation of Kefir Intake Preferences and Eating Habits in Animal Models

Before and after the experiment was confirmed whether the kefir preference and the food pipe change of the animal model in accordance with Example <2-2>.

As a result, the animal model autonomously ingested kefir when kefir was provided. Interestingly, kefir was actively consumed with great interest. In addition, most of the 200 ml of kefir provided daily was consumed for a short time (Table 3), and some animal models were excited when trying to give kefir and showed very desired behavior.

On the other hand, the animal model ingested almost all of the feed provided, confirming that the intake of kefir did not affect the feeding of dogs.

No. Feed
(g / day) Feed intake
(g / day) Kefir Served
(Ml / day) Kefir Intake
(Ml / day) Intake time
(minute) One 200 190 2.5 660 660 2 200 200 2.3 920 822.7 3 200 161 2.7 780 780 4 200 200 1.1 780 780 5 200 195 1.5 420 420 6 200 182.1 3.5 780 780 Mean ± SD 200.00 ± 0.00 188.02 ± 14.86 2.27 ± 0.86 723.33 ± 169.90 707.12 ± 150.96

Example 5 Confirmation of Fecal Quality Changes in Animal Models

Whether the fecal quality of the animal model before and after the experiment was confirmed in accordance with the above <Example <2-3>.

As a result, after 14 days of kefir intake, although the Bristol score slightly increased visually, there was no significant difference. It was confirmed that the increase was ( p <0.05, Table 4), and also no occult blood reaction was observed before and after the experiment.

No. Fecal moisture content (%) Bristol score Fecal occult blood Day 0 Day 14 Day 0 Day 14 Day 0 Day 14 One 62.06 72.68 4 4 N N 2 63.70 68.49 2 4 N N 3 54.35 72.91 4 4 N N 4 61.09 68.73 3 3 N N 5 68.07 70.29 4 4 N N 6 59.77 71.07 4 3 N N Mean ± SD 62.51 ± 4.14 70.70 ± 1.73 3.5 ± 0.84 3.67 ± 0.52 0/6 0/6 p value 0.01 0.70 - -

 That is, through the above results, it was confirmed that intake of kefir tends to soften the stool of the dog and does not cause inflammation or bleeding in the intestinal tract.

Example 6 Confirmation of Changes of Intestinal Microbiota in Dogs According to Kefir Intake

Before and after the experiment was confirmed whether the change in the intestinal microbiota of the animal model according to the above <2-4>.

As a result, after ingesting kefir, the Firmicutes / Bacteroidetes ratio, which is the closest intestinal microbial indicator to obesity, decreased (Figure 1), and Lactobacillaceae, which is generally known to control systemic inflammation and obesity caused by pathogens by acidifying the intestinal environment It was confirmed that the increase (Fig. 2). In addition, prevotella copri, which is known to compete with the representative obesity-inducing bacterium Bacteroides thetaiotaomicron, was significantly increased (p <0.05, FIG. 3). Faecalibacterium prausnitzii, which is known to increase in obese individuals, was significantly increased. The decrease was confirmed (p <0.05, FIG. 4).

That is, through the above results, kefir reduces the bacteria that can promote obesity among the bacteria present in the dog's intestine and increases the bacteria that inhibit obesity, thus preventing obesity when ingesting kefir in the long term. Or it could be improved.

So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown not in the foregoing description but in the claims, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (5)

In the food composition for preventing or improving companion animal occult blood and constipation containing kefir,
The pet is a dog
Food composition for preventing or improving companion animal occult blood and constipation.
The method of claim 1,
The kefir is orally administered at 160 to 200ml / day pet composition occult blood and constipation preventing or improving food composition.
The method of claim 1,
The kefir is to increase the growth of Lactobacillaceae bacteria and Prevotella copri bacteria in the intestine of the dog
Food composition for preventing or improving companion animal occult blood and constipation.
Ingesting the composition of claim 1 in a dog
How to treat dog's occult blood and constipation.
In the feed additive for preventing or improving constipation containing kefir,
The feed is a dog forage feed
Feed additives for preventing or improving companion animal occult blood and constipation.

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