KR20210082914A - method of preparing animal food composition against obesity with fermented sweet potato - Google Patents

Abstract

The present invention relates to a food composition for companion animals including a fermented sweet potato. The present invention prevents or alleviates obesity and constipation of companion animals.

Description

Method of preparing a food composition for companion animals for improving obesity using fermented sweet potato {method of preparing animal food composition against obesity with fermented sweet potato}

The present invention relates to a food composition for companion animals comprising fermented sweet potato.

Companion animals live together with people and provide psychological stability and intimacy. According to statistics in 2015, 21.8% of households raising companion animals accounted for about 4.57 million households, and it is increasing every year. As the most frequently bred companion animals, dogs accounted for 19.1% and cats with 5.2%, and the size of the companion animal-related market is continuously increasing. Therefore, it is expected that the market for health functional feed composition as well as general feed will be rapidly expanded.

While the companion animal market is increasing, obesity and diabetes caused by excessive nutritional intake and lack of exercise due to the misunderstanding of guardians is a very important problem in terms of animal health and welfare due to the causes of arthritis, bladder and urinary tract infections, and deterioration of kidney and liver function. is being talked about In order to solve this problem, a number of techniques for diet control of companion animals have been reported. A method for preparing a feed composition in the form of pellets using ginseng berries (application number: 10-2014-0059656) and a method for preparing a pet anti-obesity composition using red ginseng gourd (application number: 10-2017-0036461) were reported. Although ginsenoside, the main functional ingredient of red ginseng gourd and ginseng berry, has an anti-obesity effect by inhibiting the differentiation of adipocytes, it does not improve the basal metabolic rate of pets with low activity levels, so it is used as an anti-obesity and anti-diabetic functional feed. is not suitable

One object of the present invention is to provide a food composition for preventing or improving companion animal obesity and constipation, including fermented sweet potato.

Another object of the present invention is to provide a method for treating obesity or constipation in companion animals by ingesting a food composition for preventing or improving companion animal obesity and constipation, including fermented sweet potato.

Another object of the present invention is to provide a feed additive for preventing or improving obesity and constipation, including fermented sweet potato.

One aspect of the present invention provides a food composition for preventing or improving companion animal obesity and constipation, including fermented sweet potato.

The fermented sweet potato contained in the food composition of the present invention can improve the palatability of food for companion animals, prevent excessive intake of food, and soften the stool to improve constipation symptoms, as well as useful intestinal flora Therefore, it can be effectively used to prevent or improve obesity and constipation in companion animals.

As used herein, the term “prevention” refers to maintaining a normal body weight or normal bowel movement status of a companion animal.

As used herein, the term "improvement" refers to weight loss of companion animals belonging to the category of obesity or an increase in the water content in feces, and includes improving or beneficially changing symptoms caused by obesity or constipation.

The food composition of the present invention may be used further comprising an appropriate carrier, excipient and diluent commonly used in food production. Carriers, excipients and diluents include, for example, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicanite, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl tolidone, water, honey, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate and mineral oil. In the case of formulation, it can be prepared using diluents or excipients such as commonly used fillers, weight agents, binders, wetting agents, disintegrants, and surfactants. When prepared as a solid preparation, at least one or more excipients, for example, starch, calcium carbonate, sucrose or lactose, gelatin, etc. may be included in the composition. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweetening agents, fragrances, preservatives, and the like may be included.

In addition, the food composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents and fillers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, it may contain fruit or vegetables for the production of natural beverages, and these components may be used independently or in combination.

The food composition of the present invention may be used as a fermented sweet potato itself or added in 0.01 to 99.99% by weight of the food composition.

The food composition of the present invention may further include a known substance that exhibits activity in the symptoms of obesity or constipation of companion animals.

The food composition of the present invention may be used alone or in combination with methods using surgery, hormone therapy, drug therapy, and/or biological response modifiers to improve symptoms of obesity or constipation in companion animals.

According to one embodiment of the present invention, the food composition may further include abalone powder. The manufacturing process of the food composition according to the present invention is shown in FIG. 1 . Specifically, the food composition according to the present invention comprises:

A) steaming and drying sweet potatoes;

B) fermenting the dried sweet potato by boiling and drying;

C) may include adding abalone powder to the fermented sweet potato.

According to one embodiment of the present invention, the abalone powder included in the food composition is

a) freeze-drying the abalone at -80 to -50°C;

b) a first grinding step of performing the freeze-dried abalone three times at a first temperature;

c) a second grinding step of performing the freeze-dried abalone twice at a second temperature; and

d) The freeze-dried abalone may be prepared by a third grinding step of performing three times at a third temperature. A method for preparing the abalone powder is shown in FIG. 2 .

According to one embodiment of the present invention, the companion animal may be a dog.

According to one embodiment of the present invention, the obesity may be caused by any one selected from the group consisting of Firmicutes, Bacteroidesthetaiotaomicron, and Faecalibacteriumprausnitzii, which inhabit the intestine of companion animals.

Since the food composition of the present invention has probiotic activity to usefully improve the intestinal microflora associated with obesity, it can be usefully used to prevent obesity in companion animals in advance or to control the weight of obese companion animals.

Another aspect of the present invention provides a method for treating obesity or constipation in companion animals by ingesting a food composition for preventing or improving companion animal obesity and constipation, including fermented sweet potato.

As used herein, the term "treatment" means that the symptoms of obesity or constipation in companion animals are improved or changed to advantage.

The provided amount of the food composition of the present invention may be provided so that the companion animal can freely consume it, but it may vary depending on the weight of the companion animal, and the amount of fermented sweet potato contained in the food composition of the present invention is the weight of the companion animal (kg). ) may be 2 ml to 20 ml, preferably 5 ml to 10 ml.

Another aspect of the present invention provides a feed additive for preventing or improving obesity and constipation, including fermented sweet potato.

As used herein, the term "feed additive" refers to a substance added to feed for improving productivity of livestock, promoting and/or maintaining the health of companion animals, or maintaining physical condition. In the present invention, fermented sweet potato may be included as an active ingredient.

The content of the feed additive contained in the feed is not particularly limited thereto, but may be 0.01 to 20.0% (w/w), preferably 0.05 to 10.0% (w/w).

The feed additive of the present invention is not limited thereto, but animals such as cows, pigs, sheep, goats, and deer with two hoofs (follicles), chickens, ducks, geese, pheasants, etc., fish such as eels and flatfish, dogs, cats It may be added to any one or more feeds of companion animals.

The feed additive of the present invention may further include an excipient. The excipient of the present invention refers to a component added to give the active ingredient an appropriate hardness or shape, or to give the active ingredient a certain amount and weight when the amount of the main ingredient is small, and to make it a size that is easy to handle. The excipient may include those commonly used in the manufacture of feed additives, but is not limited thereto, and may include any one or more of wheat bran, corn powder, grain starch, silica powder, and diatomaceous earth, and based on feed, etc. and excipients recognized by standards (Ministry of Agriculture, Food and Rural Affairs Notice No. 2014-106).

The feed additive of the present invention may be prepared in a conventional formulation in the art, and may be implemented in the form of a powder or a pellet having a predetermined shape, but is not limited thereto. The feed additive may be powdered to enhance absorption in the body, or may be prepared in a fat-coated formulation to enhance antibacterial activity in the small intestine.

The excipient may be included in an amount of 50 to 99 parts by weight based on 100 parts by weight of the total feed additive.

The companion animal food composition of the present invention has the effect of alleviating constipation in companion animals by the probiotic effect of the fermented sweet potato and the effect of reducing the index microorganisms in the intestine related to obesity, so it can be usefully used for food such as snacks for companion animals. have.

1 is a schematic diagram showing the manufacturing process of a food composition according to the present invention.
Figure 2 is a schematic diagram showing the grinding process of the abalone process included in the food composition according to the present invention.
3 shows an indicator of changes in the intestinal microflora in an animal model.

Hereinafter, various examples are presented to help the understanding of the invention. The following examples are provided for easier understanding of the invention, and the protection scope of the invention is not limited to the following examples.

<Example>

Example 1. Preparation of a food composition comprising fermented sweet potato

A food composition containing fermented sweet potato according to the method shown in FIG. 1 and a food composition additionally containing abalone powder prepared according to the method shown in FIG. 2 were prepared.

Example 2. Method of preparation and evaluation of animal models

<2-1> Animal model experimental group design

Healthy adult dogs (n=6, 4 males, 2 females) with an age of 3 to 8 years (mean age 5.17 ± 2.32) and a body weight of 24.2 to 32.0 kg (mean BW 28.65 ± 2.94 kg) were used. The dogs used in the experiments were Mongrel, Poodle, Marinois, Labrador and Shepherd breeds. Dogs were housed for 14 days in individual cages where fresh water and food were provided and where they could exercise. As food, commercially available pelleted dog food (Natural balance Ultra Premium (Burbank, CA) and Natural Balance Pet Foods® (Dick Van Patten)) was supplied according to body weight. No other snacks were provided except feed, and data before (0 days) and after (14 days) ingestion of fermented sweet potatoes were compared. During the experiment period, a total of 200 ml of fermented sweet potatoes were provided so that they could autonomously ingest once a day.

<2-2> Fecal quality inspection

Changes in fecal quality of animal models on days 0 and 14 of the experiment were analyzed. Specifically, fecal samples were scored as shown in Table 1 below according to Bristol's scoring guidelines.

score shape One cake 2 lumpy sausage shape 3 Sausage form with cracks on the surface 4 Soft and tender sausage or snake form 5 smooth edge 6 soft edges with irregular edges 7 liquid stool

1 g of the fecal sample was taken and the fecal moisture content was measured. The fecal moisture content was calculated according to the following formula after drying in a drying oven at 80° C. for 24 hours.

100 × (weight of feces before drying - weight of feces after drying) / weight of feces before drying

Meanwhile, fecal occult blood test was performed using the Hema-Screen Lab Pack (Stanbio Laboratory, Boerne, TX, USA) according to the manufacturer's instructions.

<2-3> Intestinal microbiota analysis

Changes in the intestinal microflora of the animal models on days 0 and 14 of the experiment were analyzed. Specifically, DNA was extracted from fecal samples using NucliSENS easyMAG equipment (bioMerieux, Marcy l'Etoile, France) according to the manufacturer's instructions. Then, using the extracted DNA, the intestinal microflora group associated with obesity was quantified using qPCR analysis. Meanwhile, the gut microbiota was also analyzed through next generation sequencing. After preparing each sequencing sample according to the Illumina 16S Metagenomic Sequencing Library protocol, quality screening was performed with PicoGreen and Nanodrop, and finally, the V3-V4 region of the 16S rRNA gene was amplified, followed by analysis.

Example 3. Confirmation of changes in fecal quality in animal models

Whether the quality of feces of the animal model before and after the experiment was changed was checked according to Example <2-2>.

As a result, after 14 days of ingestion of fermented sweet potato, the Bristol score slightly increased, but did not show a significant difference. However, as a result of fecal moisture content analysis, the fecal moisture content decreased due to ingestion of fermented sweet potato. It was confirmed that there was a significant increase (p < 0.05, Table 2), and no occult blood reaction was observed both before and after the experiment.

No. Fecal moisture content (%) Bristol score occult blood in feces Day 0 Day 14 Day 0 Day 14 Day 0 Day 14 One 62.06 72.68 4 4 N N 2 63.70 68.49 2 4 N N 3 54.35 72.91 4 4 N N 4 61.09 68.73 3 3 N N 5 68.07 70.29 4 4 N N 6 59.77 71.07 4 3 N N Mean ±SD 62.51±4.14 70.70±1.73 3.5±0.84 3.67±0.52 0/6 0/6 p value 0.01 0.70 - -

That is, through the above results, it was confirmed that ingestion of fermented sweet potato tends to soften the dog's stool, and does not induce intestinal inflammation or bleeding.

Example 4. Confirmation of changes in the intestinal microflora of dogs according to ingestion of fermented sweet potatoes

Before and after the experiment, changes in the intestinal microflora of the animal model were checked according to Example <2-3>.

As a result, after ingesting fermented sweet potatoes, the Firmicutes/Bacteroidetes ratio, which is the most closely related intestinal microbial index to obesity, decreased, and Lactobacillaceae, which is known to control systemic inflammation and obesity caused by pathogens, by acidifying the intestinal environment in general, increased. Confirmed. In addition, it was confirmed that Prevotella copri, which is known to compete in the intestine with Bacteroides thetaiotaomicron, a representative obesity-inducing bacterium, increased significantly (p<0.05), and Faecalibacterium prausnitzii, which is known to increase in obese individuals, was significantly decreased. (p<0.05) (Fig. 3).

That is, through the above results, the fermented sweet potato reduces the bacteria that can promote obesity among the bacteria present in the dog's intestine and increases the bacteria that inhibit obesity, so that when fermented sweet potato is consumed in the long term, obesity is prevented Or it was confirmed that it can be improved.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (3)

A) steaming and drying sweet potatoes;
B) fermenting the dried sweet potato by boiling and drying;
C) adding abalone powder to the fermented sweet potato
A method of producing a food composition for preventing or improving companion animal obesity and constipation, including fermented sweet potato,

According to claim 1,
The abalone powder is
a) lyophilizing the abalone at -80 to -50 °C;
b) a first grinding step of performing the freeze-dried abalone three times at a first temperature;
c) a second grinding step of performing the freeze-dried abalone twice at a second temperature; and
d) characterized in that it is prepared by the third grinding step of performing the freeze-dried abalone three times at a third temperature,
A method of manufacturing a food composition for preventing or improving companion animal obesity and constipation, including fermented sweet potato.
According to claim 1,
wherein the composition comprises one or more carriers, excipients, diluents, fillers, anti-aggregating agents, lubricants, wetting agents, flavoring agents, emulsifying agents or preservatives,
A method of manufacturing a food composition for preventing or improving companion animal obesity and constipation, including fermented sweet potato.

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