KR102133811B1 - Poultry meat snack composition for pet's health care and its manufacturing method - Google Patents

Abstract

The present invention relates to a poultry snack composition for health management of pets and a manufacturing method thereof. To this end, the poultry snack composition comprises: chicken liver, chicken breast, duck breast, chicken heart, duck heart, turkey breast, quail, and pigeon breast; Bifidobacterium lactis, Bifidobacterium animalis, Lactobacillus acidophilus, Bifidobacterium longum, Bifidobacterium bifidum, Lactobacillus casei, and Enterococcus faecium; and purified nepetalactone.

Description

POULTRY MEAT SNACK COMPOSITION FOR PET'S HEALTH CARE AND ITS MANUFACTURING METHOD}

The present invention relates to a poultry meat snack composition for the health care of pets and a method of making the same.

In modern society, with the acceleration of nuclear familyization and the increase of single lives, the portion of pets in life has increased significantly, and reflecting this phenomenon, pets are called companion animals.

Since the melamine wave in 2007 when Chinese feed companies manufactured and delivered feed with melamine that was not included in the raw material stability evaluation for the purpose of showing high protein content, interest in reproduction of pets has increased. Raw food is being recognized as an alternative to alleviate anxiety about feed because it is possible to visually check what raw materials are made compared to processed feed, and additives are not used and processing that can cause severe changes in ingredients is not performed.

The advantage of reproduction is that the digestibility of the unprocessed protein is high, so that high-quality protein can be consumed, and nutrients of the raw material are well preserved without processing. Known, fresh feed sales in the United States exceeded 600 billion won and are growing rapidly by 70% every year.

However, there are many points to pay attention to because it has many advantages. The biggest problem is likely to be exposed to various harmful bacteria, such as Salmonella and E. coli, during the reproductive process. In 2012, the US FDA survey revealed that 15 of Salmonella and 32 Listeria were detected among 196 reproductive products on the market, and the FDA issued a statement stating that'I think reproduction is not a good public health method'. European veterinarians have also issued a statement stating that reproduction is not recommended due to several health and public health problems.

In addition, nutritional balance problems may occur. According to a 2013 study conducted by the UC DAVIS Veterinary Medical College in the United States, there are insufficient essential nutrients in over 200 reproductive recipes introduced in 34 different books and the Internet. It met the standards of the Association of American Feed Control Officials, and only five met the standards of the National Research Council (NRC). AAFCO and the NRC are the leading US agencies that manage the nutritional provisions of dogs and cats.

Most of the recipes made by experts are very likely to be in an unbalanced state, and in the short and short term, the nutritional imbalance is short-term and short-term in that pets can only eat food provided by humans, such as various mineral imbalance problems such as calcium excess. It can lead to serious illness.

For this reason, experts emphasize that even if they reproduce, they must carefully select specialized companies and pay attention to hygiene management. In addition, reproducing causes a large amount of time and money, and there is a problem that it is up to 8 to 9 times more expensive than commercially available feed.

To solve this problem, freeze-dried snacks and the like are distributed on the market, but unlike dogs, cats have a difficult taste, so the preference for these freeze-dried snacks is very low, and the moisture content is low, resulting in problems related to digestion and bowel movements. Therefore, additional research is required.

Korean Registered Patent No. 10-2026042

An object of the present invention is to provide a poultry meat food composition and a method of manufacturing the same while being able to provide a balanced nutrient while also significantly reducing the risk of bacterial infection.

Another object of the present invention is a poultry meat food composition having an effect capable of significantly lowering the risk of disease transmission by killing disease-causing bacteria that are transmitted through a pet, while keeping the advantages of reproduction as it is, and furthermore, the production thereof. Is to provide a way.

One aspect of the present invention for solving the above problems is to prepare a freeze-dried meat (hereinafter referred to as "poultry meat") one or more selected from the group consisting of chicken, duck, turkey, quail and pigeon, food for pets Provided is a composition.

Specifically, the poultry meat may be chicken liver, chicken breast, duck breast, chicken heart (salt), duck heart (salt), turkey breast, quail or pigeon breast, but is not limited thereto.

Specifically, the pet food composition for Bifidobacterium lactis (Bifidobacterium lactis), Bifidobacterium Annie Marlies (Bifidobacterium animalis), Lactobacillus know even Phil Ruth (Lactobacillus acidophilus), Bifidobacterium ronggeom (Bifidobacterium longum ), Bifidobacterium bifidum , Lactobacillus casei and Enterococcus faecium may further include one or more selected probiotics selected from the group consisting of, It is not limited.

In the present invention, the “pet” is also referred to as a companion animal, and refers to an animal that lives together with a person and gives psychological stability and intimacy, and includes all reptiles, birds, reptiles, fish, insects such as dogs and cats. May be, specifically, it may mean a dog or cat, but is not limited thereto.

In the present invention, the "probiotics (probiotics)" refers to a strain that has a beneficial effect on the intestinal environment when ingested and reaches the intestine. Most of the probiotics known to date are lactic acid bacteria, which is a generic term for bacteria that ferment sugars to obtain energy and produce large amounts of lactic acid. In human gut, about 1kg of bacteria are inhabited, and the amount of food and the amount of bacteria are almost the same, and the contents of feces excreted daily account for about 40% of bacteria except moisture. It is known that probiotics in the colon have a remarkable effect on the prevention and treatment of colon diseases. In particular, it is known that treatment effects can be expected by administering lactic acid bacteria to patients with colorectal cancer and colon disease, including “New Korean lactic acid bacteria that inhibit the activity of harmful enzymes in the intestine and uses thereof (KR 10-0232639 B1) ”And “Nano-type kimchi lactic acid bacteria composition (KR 10-2016-0084822 A) having an effect on improving the intestinal environment”, techniques for using probiotics containing lactic acid bacteria as a therapeutic agent for colon disease have been developed. In addition, a number of studies have revealed that these probiotics have a great influence on the health status of pets.

Probiotics used in the present invention may be used to mean microorganisms that have beneficial effects on pets, particularly cats, and specifically Bifidobacterium lactis helps to stimulate an immune response, Bifidobacterium Animalis helps protect the gastrointestinal tract and promote overall health, Lactobacillus acidophilus helps maintain the health of the entire digestive tract, and Bifidobacterium long gum maintains optimal digestive function and immunity It serves to help improve health. In addition, Bifidobacterium bifidum promotes the balance of microflora in the intestine, Lactobacillus casei promotes the growth of intestinal friendly bacteria, and Enterococcus fascium has the effect of improving the pet's feces.

Specifically, the food composition is 24 parts by weight of chicken liver, 68 parts by weight of chicken breast, 54 parts by weight of duck breast, 45 parts by weight of chicken heart (salt), 27 parts by weight of duck heart (salt), 46 parts by weight of turkey breast, quail 20 parts by weight, pigeon breast 15 parts by weight, Bifidobacterium lactis 29 parts by weight, Bifidobacterium animalis 15 parts by weight, Lactobacillus acidophilus 14 parts by weight Part, 28 parts by weight of Bifidobacterium longum , 25 parts by weight of Bifidobacterium bifidum , 16 parts by weight of Lactobacillus casei , 16 parts by weight of Enterococcus faecium Parts by weight; And purified nepetalactone (nepetalactone) may be prepared to include 0.3 to 1.5 parts by weight, and more specifically, may be prepared including 0.9 parts by weight of nepetalactone, but is not limited thereto.

In addition, the food composition may be prepared by further comprising 1 to 15 parts by weight of white spotted flower larvae known as edible insects, 1 to 15 parts by weight of Dongae, and 1 to 15 parts by weight of brown legs.

The “white spotted flower radish ( Protaetia brevitarsis )” is one of the edible insects, belonging to the Coleoptera, Chafer, and Flower Aphidaceae, which are called "manufacturing" or "slugs" in traditional Chinese medicine such as folk and Donguibogam. , 17~22 mm long, 12-15 mm wide herbivorous insect, dark coppery, shiny, and yellowish white scattered. The white spotted flower radish has long been used as a herbal medicine for the treatment of liver disease and the like, and has recently attracted great attention as an insect resource for exploration and development of useful bioactive substances.

The “Dongae- e-ae ( Ptecticus tenebrifer )” has a body length of 13 to 20 mm and the overall color is black. The face is dark brown with a very short length, the forehead is small, distinctly raised, and yellow. Antenna dark brown, bumps on shoulders and ridges on lateral edges yellowish brown, wings black. The small shield plate is short and the rear edge is obtuse. The wings are large and cloudy, and the legs are elongated and black. The base of the ankle joint of the middle leg and the base of the calf joint of the forelimb are yellow red.

The "Brown's Bee ( Tenebrio molitor )" is an insect of the Coleoptera family, also known as the Brown Rice Bee, and is distributed throughout Korea and around the world. It is an insect that undergoes complete metamorphosis through the period of eggs, larvae, pupae, and adults. There are breeding farms that are mass-produced in. The larval period is about 10 weeks, but about 2 weeks for artificial breeding.

Unlike other insects, the brown beetle is kept as a pet because it has a relatively short metamorphosis period, and the larva of the brown beetle is called a worm, and is used as a food insect. It has a high protein content, high growth rate, and very high breeding rate in animals. Because it is known. In addition, it is suitable for intensive processing because it is adaptable and easy to learn breeding techniques, so it is suitable for intensive processing because it can be reared at a factory or distributed, and can be reared in four seasons regardless of season.

Specifically, in the present invention, the food composition for a pet of the present invention shows an improvement in digestion of a pet (Experimental Example 1) and a growth inhibitory effect of a cat-mediated disease-causing bacterium (Experimental Example 3). In 3, it was confirmed through a specific experiment.

In particular, the cat-mediated disease-causing bacteria are bacteria mixed with the cat's saliva and cause toxoplasma that is infected from feces of cats, Bartonella henselae , which causes Cat Scratch Disease through acts such as scratching . Toxoplasma gondii , and refers to one or more of Corynebacterium ulcerans , which are known to cause respiratory distress, and when the composition of the present invention is continuously fed, the killing of the harmful bacteria It confirmed that it showed an effect.

In the present invention, the food means a natural product or a processed product containing one or more nutrients, and preferably means a state in which a pet can directly eat it after a certain processing process. By meaning, it is intended to include all beverages, food additives and beverage additives.

Food of the present invention, for example, various foods, beverages, gum, tea, and the like. The food, beverage, food additive and beverage additive can be prepared by a conventional manufacturing method.

In the present invention, the drink means a generic term for drinking to relieve thirst or to enjoy a taste, and is intended to include a health functional beverage. The beverage is an essential component in the indicated proportions, and there is no particular restriction on other components other than including the slug extract as an active ingredient, and may contain various flavoring agents or natural carbohydrates, etc., as additional components, as in conventional beverages. .

Examples of such natural carbohydrates include monosaccharides, such as disaccharides such as glucose and fructose, such as maltose, sucrose, etc. and polysaccharides, such as conventional sugars such as dextrin, cyclodextrin, and the like. Sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatine, stevia extract (for example, rebaudioside A, glycyrrhizine, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate may be generally about 1 to 20 g, preferably 5 to 12 g per 100 ml of the composition of the present invention.Otherwise, the composition of the present invention is for preparing natural fruit juice, fruit juice beverage, and vegetable beverage. It may further contain the flesh for.

In addition to the above, the food composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and neutralizing agents (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and It may contain salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonic acid used in carbonated beverages, and the like. These components can be used independently or in combination.

In addition, in the present invention, the food composition may be used as a food additive that can be added to pet food or as an anthelmintic agent for killing bacteria present in the pet.

Specifically, the food additive can be used alone or in combination with calcium formate, which is well known in the art, and the effect of the food additive improves the growth rate of pets, improves the food conversion rate, and reduces the frequency of diarrhea. It may have the same effect as the commonly known effect.

Another aspect of the present invention, (a) Bifidobacterium lactis ( Bifidobacterium lactis ) 29 parts by weight, Bifidobacterium animalis ( Bifidobacterium animalis ) 15 parts by weight, Lactobacillus acidophilus ( Lactobacillus acidophilus ) 14 parts by weight Part, 28 parts by weight of Bifidobacterium longum , 25 parts by weight of Bifidobacterium bifidum , 16 parts by weight of Lactobacillus casei and 16 parts by weight of Enterococcus faecium Preparing a probiotic mixed powder comprising a probiotic mixture composed of parts by weight; (b) Soak chicken liver, chicken breast, duck breast, chicken heart (salt), duck heart (salt), turkey breast, quail and pigeon breasts in diluted vinegar or electrolyzed water for 30 minutes and then freeze at -5°C. step; (c) 24 parts by weight of frozen chicken liver, 68 parts by weight of chicken breast, 54 parts by weight of duck breast, 45 parts by weight of chicken heart (salt), 27 parts by weight of duck heart (salt), 46 parts by weight of turkey breast, 20 parts of quail Parts by weight, 15 parts by weight of pigeon breast cut into a cube shape of 1.2cmx1.2cmx1.5cm, 0.3 to 1.5 parts by weight of nepetalactone; And administering 30 parts by weight of the probiotic mixed powder prepared in the step (a) to mix together, and then left to stand at 20 to 28° C. for 45 minutes to 1 hour for primary aging; (d) aging at a low temperature for 2 to 30 hours at 4 to 6° C. after the first aging is completed; (e) after the second low temperature aging is completed, rapidly frozen to -40 to -50°C and maintained for 24 hours, then put in a freeze-drying chamber and freeze-dried at 30 to 40°C for 24 to 48 hours; And (f) adding 1 to 5 parts by weight of the probiotic mixed powder; a method of manufacturing a pet food composition may be provided, but is not limited thereto, and may be appropriately changed as necessary.

Specifically, the probiotic mixture powder of the step (a) is 3 parts by weight of pectin, 2 parts by weight of fructooligosaccharide, 3 parts by weight of albinogalactan, 2 parts by weight of starch, 2 parts by weight of skim milk, 2 parts by weight of glucose and 3 parts of lactose Prebiotics mixture consisting of parts by weight; may further include, but may be prepared by mixing at 1300 to 2500rpm for 2 to 5 minutes in a shaker, but is not limited thereto.

In the present invention, the term “prebiotics” refers to a component in food that promotes or activates the growth of beneficial microorganisms in the intestine. The most well-known dietary fiber is a substance that is not digested in the small intestine but goes down to the large intestine and feeds the beneficial bacteria that live there. In addition, oligosaccharides such as fructan and galactan are known as prebiotics.

Another aspect of the present invention provides a pet food composition prepared by the above manufacturing method.

The poultry meat food composition provided by the present invention can mix various types of poultry meat in an optimal formulation, and further mix fruits, berries, vegetables, meat, fish, insects, yeast, and fungi to provide a balanced nutrient. By promoting the growth of pets, it can be stored for a long time while maintaining the benefits of reproduction.

In addition, it shows high preference according to the cat's demanding taste, and can promote health care by promoting digestion and defecation processes.

Furthermore, the poultry meat food composition of the present invention significantly lowers the risk of bacterial infection that may occur during the production process of reproduction, and has the effect of killing the bacteria transmitted by the cat, just by ingesting the poultry meat food composition of the present invention. It can have many beneficial effects, such as lowering the risk of pet owners' disease infection.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the invention.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only to illustrate the present invention, the present invention is not limited by the following examples.

Manufacturing example. Preparation of probiotic mixed powder

In order to prepare the poultry meat food composition of the present invention, a probiotic mixed powder for mixing with raw meat was prepared.

The probiotic mixed powder was prepared including 7 types of probiotics and prebiotics.

Specifically, probiotics added to the probiotic mixed powder include Bifidobacterium lactis , Bifidobacterium animalis , Lactobacillus acidophilus , and Bifidobacterium long gum ( Bifidobacterium longum ), Bifidobacterium bifidum , Lactobacillus casei and Enterococcus faecium .

The probiotic refers to a microorganism having a beneficial effect, Bifidobacterium lactis helps to stimulate the immune response, and Bifidobacterium animalis helps protect the gastrointestinal tract and promote overall health, , Lactobacillus acidophilus helps maintain overall health of the digestive tract, and Bifidobacterium long gum helps maintain optimal digestive function and improve immune health. In addition, Bifidobacterium bifidum promotes the balance of microflora in the intestine, Lactobacillus casei promotes the growth of intestinal friendly bacteria, and Enterococcus fascium has the effect of improving the pet's feces.

The probiotic mixed powder is Bifidobacterium lactis 29 parts by weight, Bifidobacterium animalis 15 parts by weight, Lactobacillus acidophilus 14 parts by weight, Bifidobacterium Probiotic mixture consisting of 28 parts by weight of Bifidobacterium longum , 25 parts by weight of Bifidobacterium bifidum , 16 parts by weight of Lactobacillus casei and 32 parts by weight of Enterococcus faecium ; And 3 parts by weight of pectin, 2 parts by weight of fructooligosaccharide, 3 parts by weight of albinogalactan, 2 parts by weight of starch, 2 parts by weight of skim milk, 2 parts by weight of glucose, and 3 parts by weight of lactose in a shaker. It was prepared by mixing at 1300 to 2500 rpm for 5 minutes.

Example 1. Preparation of poultry meat food composition treated with probiotics

The poultry meat food composition of the present invention was prepared through the following process.

First, fresh chicken liver, chicken breast, duck breast, chicken heart (salt), duck heart (salt), turkey breast, quail and pigeon breasts are diluted in diluted vinegar or electrolytic water for 30 minutes, sterilized, and then -5℃ Prepared by freezing. At this time, the frozen meat was used after being sufficiently thawed in a clean state.

Thereafter, 24 parts by weight of frozen chicken liver, 68 parts by weight of chicken breast, 54 parts by weight of duck breast, 45 parts by weight of chicken heart (salt), 27 parts by weight of duck heart (salt), 46 parts by weight of turkey breast, 20 parts by weight of quail , Pigeon breasts 15 parts by weight of 1.2cmx1.2cmx1.5cm cut into cubes and prepared, then 0.9 parts by weight of purified nepetalactone and 30 parts by weight of the probiotic mixture powder prepared in the above preparation are mixed together Thereafter, the mixture was left to stand at 20 to 28°C for 45 minutes to 1 hour, and then first aged, and then 4 to 6°C was subjected to a second low temperature aging step for 12 to 30 hours.

After the second low temperature aging was completed, it was rapidly frozen at -40 to -50°C and maintained for 24 hours, and then placed in a freeze-drying chamber and freeze-dried at 30 to 40°C for 24 to 48 hours.

After the freeze-drying treatment was completed, 1 to 5 parts by weight of the probiotic mixed powder prepared in the preparation example was further added to finally prepare a poultry meat food composition of the present invention.

Experimental Example 1. Experiment to check the preference and digestion status of poultry meat food composition

Cats are difficult to reproduce because of their demanding taste, and in particular, a low preference for freeze-dried food occurs, so the preference for the poultry food composition of the present invention was confirmed. Furthermore, when ingesting the composition of the present invention, it was confirmed whether it is helpful in maintaining health by checking the state of digestion and excrement.

Specifically, the poultry food composition of the present invention was fed twice a day for 2 weeks to 12 cats having different genders, weights, and ages, and the results after the initial time point, 1 week, and 2 weeks were confirmed.

The preference was given to the cat owner by rating how much the cat likes the food of the present invention and giving it a score.

Digestion and fecal condition check were made if the hardness (hardness) of the feces was very hard or too dilute, so that 1 point was given to all cases of diarrhea, and if it was too hard or too dilute and soft enough, it was set to 5 points.

Separately, feces were collected at the initial time point, 1 week and 2 week time points, and probiotic detection in the feces and ammonia concentration, lactic acid concentration, valeric acid concentration, and acetic acid concentration were measured.

Regarding preference, 2 cats out of 12 cats were not interested (0 points), 1 was low (1~2 points), and 9 were higher than normal (3~5 points). It was confirmed that the preference was shown. This is thought to be because the poultry meat food composition of the present invention is evenly mixed with various poultry meats, and can not only encompass various tastes of each individual to some extent, but also contains nepetalactone, which is a main component of a highly preferred cat food. However, because there are some cats that do not respond to these cats, there appear to be cats (3 cats) with a preference of 0 to 2.

Table 1 below shows the results of confirming the digestion and fecal state after two weeks of feeding on 9 cats with moderate or higher preference. The excrement hardness showed the average value of 9 animals, and whether to detect probiotics is given from 0 to 7 points of how many of the 7 types of probiotics prepared in the above-mentioned mixed powder were detected, and their average values It was described.

division excreta
Hardness (average) Whether to detect probiotics (average) Ammonia concentration Lactic acid
density Valeric acid concentration Acetic acid concentration 0 weeks 2.3 1.2 - - - - 1 week 3.6 5.5 decrease decrease decrease increase 2 weeks 4.8 6.1 decrease decrease decrease increase

As shown in Table 1, as the food of the present invention was fed, it was confirmed that the hardness of the feces was increased to nearly 5 points, and the detection of probiotics also increased the cats in which all 7 species were detected as the number of parking lots increased. Was confirmed. In addition, while the content of substances harmful to the cat's digestive system, such as ammonia, lactic acid, and valeric acid, was decreased, the concentration of acetic acid increased, confirming that the activity of lactic acid bacteria in the intestine is active. Further, after stopping the food feeding of the present invention to the 9 cats, the feces were collected one more time after 2 weeks, and as a result, the average of 3 probiotics was only detected in less than half of the 4 cats. Significantly fewer beneficial microorganisms in the intestine were detected compared to when the food of the present invention was administered.

Experimental Example 2. Storage stability confirmation experiment of poultry meat food composition

The poultry meat food composition prepared by the method of the present invention shows a significantly improved storage property when compared to the conventional raw food or simple freeze-dried raw food, and the storage stability comparison with the poultry food composition of Example 1 of the present invention was evaluated. .

As a control group, a commercially available dry processed feed was used, and Comparative Example 1 was mixed with the probiotic mixed powder as Comparative Example 1 in which both the probiotic mixed powder and the freeze-drying treatment were not performed during the preparation process of Example 1. The freeze-drying treatment was set as Comparative Example 2, and the freeze-drying treatment without probiotic mixed powder treatment was set as Comparative Example 3, and the storage stability was evaluated under accelerated conditions of 70% humidity at 45°C, and the results were shown in Table 2. Shown.

Specifically, storage stability was evaluated by a 5-point rating method, and the criteria are as follows.

1 point: there is a lot of water, it is soft, and the smell is severe, 2 points: a bit of water and a little odor are left, 3 points: a little bit of water is formed, and the shape is slightly deformed, 4 points: a little water is formed, 5 Point: Little change.

division Example 1 Control Comparative Example 1 Comparative Example 2 Comparative Example 3 1 hours 4.9 4.8 4.3 4.5 4.8 2 hours 4.5 4.6 3.6 4.0 4.2 3 hours 4.2 4.3 3.0 3.4 3.5 4 hours 3.8 3.9 2.3 2.8 3.1 5 hours 3.6 3.5 1.8 2.5 2.5 6 hours 3.4 3.2 1.5 2.2 2.4

As shown in Table 2, it was confirmed that the poultry meat food composition prepared by the method of the present invention exhibits storage stability similar to that of the processed dry feed as well as the comparative example. In addition, it showed higher stability than Comparative Example 3, which is a freeze-dried composition without treatment with probiotics, and when comparing the results of Comparative Examples 1 and 2, it was confirmed that storage stability was significantly increased when probiotic treatment was performed.

Experimental Example 3. Cat-mediated disease-causing bacteria growth inhibition experiment

When feeding the food composition of the present invention, to determine whether it is possible to inhibit the growth of disease-causing bacteria present in the cat's body, whether harmful bacteria known as bacteria that are present in the body of pets such as cats and transmit disease to humans are reduced. Was confirmed.

Specifically, cat-scratch disease through an act of will, such as scratching a cat bacteria mixed with saliva (Cat Scratch Disease) induced bato Nella (Bartonella henselae) bacterium Toxoplasma that causes toxoplasmosis are infected from the faeces of cats Cryptosporidium (Toxoplasma, which gondii ) and corynebacterium ulcerans , which are known to cause respiratory distress, respectively, to obtain cats infected with Corynebacterium ulcerans , respectively. It was confirmed whether the induced bacteria were reduced.

Similar to the method of Experimental Example 1, the cat was fed twice a day for 2 weeks, and the feces at the first time point, 1 week, and 2 weeks were examined. Extract the area with stool, blood, and mucus from the stool, transfer 50-200mg of sample into a 1.5ml tube, and add 400ul of Stool lysis buffer (Bioneer, Korea) and 10ul of protease K. mg/ml was added. Thereafter, the tube was vortexed strongly for 1 minute, and then incubated for about 30 minutes until the specimen was completely released in a shaking incubator at 60°C. After the sample was completely dissolved, centrifugation was performed at 12,000 rpm for 5 minutes, the supernatant was taken, inoculated into MRS broth, and the results of incubation at 37° C. for 12 hours were shown in Table 3 below.

division Number of bacteria (CFU/g) Batonella Toxoplasmosis Corynebacterium urcerans 0 weeks 8.2×10 ⁵ 8.9×10 ⁵ 5.8×10 ⁴ 1 week 3.2×10 ⁴ 6.7×10 ³ 4.5×10 ³ 2 weeks 1.9×10 ² 1.3x10 ¹ 1.8×10 ²

As shown in Table 3, when the composition of Example 1 of the present invention was fed, it was confirmed that it exhibited a marked reduction effect on all disease-causing bacteria known to exist in the cat's body. In particular, it was confirmed that the reduction effect of Toxoplasma gondii is very excellent. The above-described description of the present invention is for illustration purposes, and a person having ordinary knowledge in the technical field to which the present invention belongs does not change the technical spirit or essential characteristics of the present invention. It will be understood that other concrete forms can be easily modified. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all modifications or variations derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present invention.

Claims (7)

Chicken liver 24 parts, chicken breast 68 parts, duck breast 54 parts, chicken heart (salt) 45 parts, duck heart (salt) 27 parts, turkey breast 46 parts, quail 20 parts, pigeon breast 15 Parts by weight;
Bifidobacterium lactis 29 parts by weight, Bifidobacterium animalis 15 parts by weight, Lactobacillus acidophilus 14 parts by weight, Bifidobacterium longum ) 28 parts by weight, Bifidobacterium bifidum (25 parts by weight), Lactobacillus casei (16 parts by weight), Enterococcus faecium ( Enterococcus faecium ) 32 parts by weight; And
Purified nepetalactone (nepetalactone) is prepared by containing 0.3 to 1.5 parts by weight of a pet food composition. delete delete According to claim 1,
The food composition is a pet food composition for improving the digestion of pets and inhibiting the growth of cat-borne disease-causing bacteria. (a) Bifidobacterium lactis 29 parts by weight, Bifidobacterium animalis 15 parts by weight, Lactobacillus acidophilus 14 parts by weight, Bifidobacterium long gum ( Bifidobacterium longum ) 28 parts by weight, 25 parts by weight of Bifidobacterium bifidum , Lactobacillus casei 16 parts by weight, and Enterococcus faecium 32 parts by weight Preparing a probiotic mixed powder;
(b) Soak chicken liver, chicken breast, duck breast, chicken heart (salt), duck heart (salt), turkey breast, quail and pigeon breasts in diluted vinegar or electrolyzed water for 30 minutes and then freeze at -5°C. step;
(c) 24 parts by weight of frozen chicken liver, 68 parts by weight of chicken breast, 54 parts by weight of duck breast, 45 parts by weight of chicken heart (salt), 27 parts by weight of duck heart (salt), 46 parts by weight of turkey breast, 20 parts of quail Parts by weight, 15 parts by weight of pigeon breast cut into a cube shape of 1.2cmx1.2cmx1.5cm, 0.3 to 1.5 parts by weight of nepetalactone; And administering 30 parts by weight of the probiotic mixed powder prepared in the step (a) to mix together, and then left to stand at 20 to 28° C. for 45 minutes to 1 hour for primary aging;
(d) aging at a low temperature for 2 to 30 hours at 4 to 6° C. after the first aging is completed;
(e) after the second low temperature aging is completed, rapidly frozen to -40 to -50°C and maintained for 24 hours, then put in a freeze-drying chamber and freeze-dried at 30 to 40°C for 24 to 48 hours; And
(f) adding 1 to 5 parts by weight of the probiotic mixed powder; a method of producing a pet food composition comprising a. The method of claim 5,
The probiotic mixed powder of step (a) comprises 3 parts by weight of pectin, 2 parts by weight of fructooligosaccharide, 3 parts by weight of albinogalactan, 2 parts by weight of starch, 2 parts by weight of skim milk, 2 parts by weight of glucose and 3 parts by weight of lactose Prebiotic mixture; further comprising a mixture of 1300 to 2500rpm for 2 to 5 minutes in a shaker, the method for producing a pet food composition. A food composition for pets, prepared by the method of claim 5.