JP6196241B2 - 生物学的に活性なポリペプチドのハイスループットスクリーニング - Google Patents

生物学的に活性なポリペプチドのハイスループットスクリーニング Download PDF

Info

Publication number
JP6196241B2
JP6196241B2 JP2014555774A JP2014555774A JP6196241B2 JP 6196241 B2 JP6196241 B2 JP 6196241B2 JP 2014555774 A JP2014555774 A JP 2014555774A JP 2014555774 A JP2014555774 A JP 2014555774A JP 6196241 B2 JP6196241 B2 JP 6196241B2
Authority
JP
Japan
Prior art keywords
cell
polypeptide
nucleic acid
beads
item
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2014555774A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015506699A (ja
JP2015506699A5 (enExample
Inventor
ローランド グリーン，
ローランド グリーン，
ブライアン グレーザー，
ブライアン グレーザー，
イバー メイバントッソン，
イバー メイバントッソン，
キンバリー カウフマン，
キンバリー カウフマン，
マディソン グリーン，
マディソン グリーン，
Original Assignee
インベンラ， インコーポレイテッド
インベンラ， インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by インベンラ， インコーポレイテッド, インベンラ， インコーポレイテッド filed Critical インベンラ， インコーポレイテッド
Publication of JP2015506699A publication Critical patent/JP2015506699A/ja
Publication of JP2015506699A5 publication Critical patent/JP2015506699A5/ja
Application granted granted Critical
Publication of JP6196241B2 publication Critical patent/JP6196241B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2014555774A 2012-02-02 2013-02-01 生物学的に活性なポリペプチドのハイスループットスクリーニング Expired - Fee Related JP6196241B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261594149P 2012-02-02 2012-02-02
US61/594,149 2012-02-02
PCT/US2013/024406 WO2013116698A2 (en) 2012-02-02 2013-02-01 High throughput screen for biologically active polypeptides

Publications (3)

Publication Number Publication Date
JP2015506699A JP2015506699A (ja) 2015-03-05
JP2015506699A5 JP2015506699A5 (enExample) 2016-03-10
JP6196241B2 true JP6196241B2 (ja) 2017-09-13

Family

ID=47750048

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014555774A Expired - Fee Related JP6196241B2 (ja) 2012-02-02 2013-02-01 生物学的に活性なポリペプチドのハイスループットスクリーニング

Country Status (5)

Country Link
US (1) US9701959B2 (enExample)
EP (1) EP2809783A2 (enExample)
JP (1) JP6196241B2 (enExample)
CA (1) CA2861387A1 (enExample)
WO (1) WO2013116698A2 (enExample)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP3913375A1 (en) 2006-01-11 2021-11-24 Bio-Rad Laboratories, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2021113A2 (en) 2006-05-11 2009-02-11 Raindance Technologies, Inc. Microfluidic devices
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
EP2622103B2 (en) 2010-09-30 2022-11-16 Bio-Rad Laboratories, Inc. Sandwich assays in droplets
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP3305918B1 (en) 2012-03-05 2020-06-03 President and Fellows of Harvard College Methods for epigenetic sequencing
US8975042B2 (en) * 2012-12-21 2015-03-10 Dna Twopointo, Inc. Fluorescent and colored proteins and methods for using them
US9290552B2 (en) 2012-12-21 2016-03-22 Dna Twopointo, Inc. Fluorescent and colored proteins and methods for using them
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
LT3456846T (lt) * 2014-04-21 2022-09-12 President And Fellows Of Harvard College Nukleorūgšties unikalios sekos įvedimo sistemos ir būdai
EP3268462B1 (en) * 2015-03-11 2021-08-11 The Broad Institute, Inc. Genotype and phenotype coupling
EP3283629A4 (en) 2015-04-17 2018-08-29 President and Fellows of Harvard College Barcoding systems and methods for gene sequencing and other applications
WO2016187466A1 (en) * 2015-05-20 2016-11-24 Invenra, Inc. Methods, apparatus and compositions for expression and high throughput screening
WO2017004250A1 (en) 2015-06-29 2017-01-05 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis
US11965891B2 (en) 2015-12-30 2024-04-23 Bio-Rad Laboratories, Inc. Digital protein quantification
JP7086856B2 (ja) * 2016-04-12 2022-06-20 メディミューン，エルエルシー 免疫レパートリー発掘
KR102657960B1 (ko) * 2016-10-18 2024-04-16 삼성전자주식회사 힘 전달 프레임 및 이를 포함하는 운동 보조 장치
JP2020500517A (ja) 2016-11-28 2020-01-16 アリゾナ ボード オブ リージェンツ オン ビハーフ オブ アリゾナ ステート ユニバーシティ 連続流小滴反応に関連するシステムおよび方法
MY201744A (en) 2016-12-23 2024-03-15 Visterra Inc Binding polypeptides and methods of making the same
GB2577214B (en) * 2017-05-05 2021-10-06 Scipio Bioscience Methods for trapping and barcoding discrete biological units in hydrogel
CN118599963A (zh) 2017-09-22 2024-09-06 华盛顿大学 细胞分子的原位组合标记
WO2019161243A1 (en) * 2018-02-15 2019-08-22 Enevolv, Inc. Sensor systems
WO2019195197A1 (en) 2018-04-02 2019-10-10 Dropworks, Inc. Systems and methods for serial flow emulsion processes
WO2020193368A1 (en) * 2019-03-22 2020-10-01 Qiagen Gmbh Method for improving the amplification efficiency of bead-based emulsion pcr (empcr)
WO2020247976A1 (en) * 2019-06-03 2020-12-10 Dropworks, Inc. Systems and methods for stabilizing emulsions
CN114829626A (zh) 2019-10-10 2022-07-29 1859公司 用于微流体筛选的方法和系统
EP4189085A4 (en) * 2020-07-28 2024-08-28 Protillion Biosciences, Inc. SYSTEMS AND METHODS FOR TESTING MULTIPLE POLYPEPTIDES
EP4200434A4 (en) * 2020-08-21 2024-10-02 Hifibio (Shanghai) Co., Ltd. FUNCTIONAL SCREENING USING DROPLET MICROFLUIDIC

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3826364A (en) 1972-05-22 1974-07-30 Univ Leland Stanford Junior Particle sorting method and apparatus
US4284412A (en) 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4498766A (en) 1982-03-25 1985-02-12 Becton, Dickinson And Company Light beam focal spot elongation in flow cytometry devices
US4661913A (en) 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4774189A (en) 1984-12-24 1988-09-27 Flow Cytometry Standards Corp. Fluorescent calibration microbeads simulating stained cells
US4767206A (en) 1984-12-24 1988-08-30 Flow Cytometry Standards Corporation Calibration method for flow cytometry using fluorescent microbeads and synthesis thereof
US4857451A (en) 1984-12-24 1989-08-15 Flow Cytometry Standards Corporation Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4989977A (en) 1985-07-29 1991-02-05 Becton, Dickinson And Company Flow cytometry apparatus with improved light beam adjustment
US4714682A (en) 1985-12-11 1987-12-22 Flow Cytometry Standards Corporation Fluorescent calibration microbeads simulating stained cells
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
AU622104B2 (en) 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
EP0359789B1 (en) 1988-01-21 1993-08-04 Genentech, Inc. Amplification and detection of nucleic acid sequences
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5858652A (en) 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
US4932207A (en) 1988-12-28 1990-06-12 Sundstrand Corporation Segmented seal plate for a turbine engine
US5160974A (en) 1990-06-25 1992-11-03 Flow Science, Inc. Closed sample cell for use in flow cytometry
US5478722A (en) 1991-02-17 1995-12-26 The Curators Of The University Of Missouri Preserved cell preparations for flow cytometry and immunology
US5846709A (en) 1993-06-15 1998-12-08 Imclone Systems Incorporated Chemical process for amplifying and detecting nucleic acid sequences
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
WO1995033073A1 (en) 1994-05-28 1995-12-07 Tepnel Medical Limited Producing copies of nucleic acids
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
WO1996006190A2 (en) 1994-08-19 1996-02-29 Perkin-Elmer Corporation Coupled amplification and ligation method
US5935825A (en) 1994-11-18 1999-08-10 Shimadzu Corporation Process and reagent for amplifying nucleic acid sequences
US5843650A (en) 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
WO1998004689A1 (en) 1995-07-31 1998-02-05 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US20030215798A1 (en) * 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
US5849546A (en) 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
US5849497A (en) 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
US5866366A (en) 1997-07-01 1999-02-02 Smithkline Beecham Corporation gidB
DK1801214T3 (da) 1997-07-07 2011-01-24 Medical Res Council In vitro sorteringsfremgangsmåde
US5916776A (en) 1997-08-27 1999-06-29 Sarnoff Corporation Amplification method for a polynucleotide
US5932451A (en) 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
US6210910B1 (en) 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB9917027D0 (en) * 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
WO2002014860A1 (en) 2000-08-15 2002-02-21 Discerna Limited Functional protein arrays
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
GB0221053D0 (en) 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
CA2513535C (en) 2003-01-29 2012-06-12 454 Corporation Bead emulsion nucleic acid amplification
US20060210982A1 (en) * 2003-01-31 2006-09-21 Hiroshi Yanagawa Cleavable assigned molecules and screening method using the same
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
EP1664265A4 (en) 2003-07-21 2009-11-25 Seng Entpr Ltd IMPROVED MULTIPLAY PLATE
US7585815B2 (en) 2003-07-24 2009-09-08 Lawrence Livermore National Security, Llc High throughput protein production screening
JP4430076B2 (ja) * 2003-10-01 2010-03-10 イーティーエイチ・チューリッヒ ポリペプチドの試験管内進化方法
GB0325653D0 (en) 2003-11-03 2003-12-10 Medical Res Council CST emulsions
ES2532608T3 (es) 2003-11-20 2015-03-30 The Board Of Trustees Of The Leland Stanford Junior University Métodos mejorados de síntesis proteica in vitro
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
GB0511717D0 (en) 2005-06-09 2005-07-13 Babraham Inst Repeatable protein arrays
WO2009145818A1 (en) * 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc Single molecule loading methods and compositions
US8309317B2 (en) 2008-04-05 2012-11-13 Single Cell Technology, Inc. Method of screening single cells for the production of biologically active agents
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US8312249B1 (en) 2008-10-10 2012-11-13 Apple Inc. Dynamic trampoline and structured code generation in a signed code environment
CN102549427A (zh) * 2009-07-07 2012-07-04 新加坡科技研究局 鉴定结合配偶体对的方法
FR2957821B1 (fr) 2010-03-24 2014-08-29 Inst Francais Du Petrole Nouvelle zone de regeneration du catalyseur divisee en secteurs pour unites catalytiques regeneratives

Also Published As

Publication number Publication date
WO2013116698A2 (en) 2013-08-08
EP2809783A2 (en) 2014-12-10
US20150018236A1 (en) 2015-01-15
US9701959B2 (en) 2017-07-11
CA2861387A1 (en) 2013-08-08
WO2013116698A3 (en) 2013-10-17
JP2015506699A (ja) 2015-03-05

Similar Documents

Publication Publication Date Title
JP6196241B2 (ja) 生物学的に活性なポリペプチドのハイスループットスクリーニング
Matuła et al. Single‐cell analysis using droplet microfluidics
Taly et al. Droplets as microreactors for high‐throughput biology
US20070077572A1 (en) Compositions and methods for in vitro sorting of molecular and cellular libraries
JP6608368B2 (ja) 核酸バーコードを用いた単一細胞と関連づけられた核酸の分析方法
US20130143749A1 (en) Methods of identifying a pair of binding partners
US20060003347A1 (en) Single-molecule in vitro evolution
US20030049841A1 (en) High throughput or capillary-based screening for a bioactivity or biomolecule
CN110520729B (zh) 用于产生和筛选隔离的肽文库的方法
JP2005501217A (ja) 生体活性または生体分子のハイスループットスクリーニングまたはキャピラリーに基づくスクリーニング
AU2002304450A1 (en) Selective gene amplification
EP1425394A2 (en) Selective gene amplification
US20150057162A1 (en) Peptide arrays
Yong et al. Rapid directed evolution of stabilized proteins with cellular high‐throughput encapsulation solubilization and screening (CHESS)
US20120124685A1 (en) Compositions and methods for gene expression and chromatin profiling of individual cell types within a tissue
WO2016187466A1 (en) Methods, apparatus and compositions for expression and high throughput screening
US20240377387A1 (en) Methods for improving sensitivity of immune profiling using oligo-tagged antigens
US20210214791A1 (en) Absolute quantification of target molecules at single-entity resolution using tandem barcoding
JP2006211984A (ja) エマルジョンを利用した核酸増幅方法、及び核酸増幅反応用キット
MXPA00011879A (es) Construccion y uso de bibliotecas catalogadas de acidos nucleicos que contienen representaciones ventajosamente ajustadas de componentes definidos..
Schwartz et al. Assembly of multi-subunit fusion proteins into the RNA-targeting type III-D CRISPR-Cas effector complex
US20250163406A1 (en) Methods for generating nucleic acid encoded protein libraries and uses thereof
US20230349901A1 (en) Methods For Identification of Cognate Pairs of Ligands and Receptors
WO2025160372A1 (en) Methods for identification of cognate pairs of ligands and receptors
JP2023511440A (ja) 複数の転写産物のハイスループット連結

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160121

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160121

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20161104

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170126

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170403

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170424

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20170728

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20170817

R150 Certificate of patent or registration of utility model

Ref document number: 6196241

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees