JP2015506699A5 - - Google Patents
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- JP2015506699A5 JP2015506699A5 JP2014555774A JP2014555774A JP2015506699A5 JP 2015506699 A5 JP2015506699 A5 JP 2015506699A5 JP 2014555774 A JP2014555774 A JP 2014555774A JP 2014555774 A JP2014555774 A JP 2014555774A JP 2015506699 A5 JP2015506699 A5 JP 2015506699A5
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- Prior art keywords
- cell
- nucleic acid
- polypeptide
- item
- acid molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004027 cell Anatomy 0.000 claims description 165
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 143
- 229920001184 polypeptide Polymers 0.000 claims description 142
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 142
- 108020004707 nucleic acids Proteins 0.000 claims description 132
- 102000039446 nucleic acids Human genes 0.000 claims description 132
- 150000007523 nucleic acids Chemical class 0.000 claims description 132
- 238000000034 method Methods 0.000 claims description 131
- 238000012360 testing method Methods 0.000 claims description 116
- 230000008512 biological response Effects 0.000 claims description 46
- 239000003094 microcapsule Substances 0.000 claims description 46
- 239000011324 bead Substances 0.000 claims description 37
- 239000000839 emulsion Substances 0.000 claims description 20
- 230000008859 change Effects 0.000 claims description 16
- 230000004044 response Effects 0.000 claims description 14
- 230000003287 optical effect Effects 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- 238000001943 fluorescence-activated cell sorting Methods 0.000 claims description 8
- 238000002372 labelling Methods 0.000 claims description 8
- 230000004071 biological effect Effects 0.000 claims description 7
- 102000003939 Membrane transport proteins Human genes 0.000 claims description 6
- 108090000301 Membrane transport proteins Proteins 0.000 claims description 6
- 230000009061 membrane transport Effects 0.000 claims description 6
- 230000001747 exhibiting effect Effects 0.000 claims description 5
- 108090000672 Annexin A5 Proteins 0.000 claims description 4
- 102000004121 Annexin A5 Human genes 0.000 claims description 4
- 241000238631 Hexapoda Species 0.000 claims description 4
- 206010028980 Neoplasm Diseases 0.000 claims description 4
- 108700019146 Transgenes Proteins 0.000 claims description 4
- 238000001261 affinity purification Methods 0.000 claims description 4
- 230000006907 apoptotic process Effects 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 201000011510 cancer Diseases 0.000 claims description 4
- 230000030833 cell death Effects 0.000 claims description 4
- 230000024245 cell differentiation Effects 0.000 claims description 4
- 230000006037 cell lysis Effects 0.000 claims description 4
- 230000004663 cell proliferation Effects 0.000 claims description 4
- 239000007850 fluorescent dye Substances 0.000 claims description 4
- 230000002538 fungal effect Effects 0.000 claims description 4
- 210000002865 immune cell Anatomy 0.000 claims description 4
- 230000003834 intracellular effect Effects 0.000 claims description 4
- 210000004962 mammalian cell Anatomy 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 210000000130 stem cell Anatomy 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 102000011727 Caspases Human genes 0.000 claims description 2
- 108010076667 Caspases Proteins 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 230000004913 activation Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 238000004020 luminiscence type Methods 0.000 claims description 2
- 230000001404 mediated effect Effects 0.000 claims description 2
- 210000002569 neuron Anatomy 0.000 claims description 2
- 230000007717 exclusion Effects 0.000 claims 1
- 239000002245 particle Substances 0.000 description 12
- 238000004581 coalescence Methods 0.000 description 4
- 230000008030 elimination Effects 0.000 description 3
- 238000003379 elimination reaction Methods 0.000 description 3
- 239000000693 micelle Substances 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000000172 cytosol Anatomy 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000010807 negative regulation of binding Effects 0.000 description 1
- 230000018791 negative regulation of catalytic activity Effects 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000018767 positive regulation of catalytic activity Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261594149P | 2012-02-02 | 2012-02-02 | |
| US61/594,149 | 2012-02-02 | ||
| PCT/US2013/024406 WO2013116698A2 (en) | 2012-02-02 | 2013-02-01 | High throughput screen for biologically active polypeptides |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015506699A JP2015506699A (ja) | 2015-03-05 |
| JP2015506699A5 true JP2015506699A5 (enExample) | 2016-03-10 |
| JP6196241B2 JP6196241B2 (ja) | 2017-09-13 |
Family
ID=47750048
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014555774A Expired - Fee Related JP6196241B2 (ja) | 2012-02-02 | 2013-02-01 | 生物学的に活性なポリペプチドのハイスループットスクリーニング |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9701959B2 (enExample) |
| EP (1) | EP2809783A2 (enExample) |
| JP (1) | JP6196241B2 (enExample) |
| CA (1) | CA2861387A1 (enExample) |
| WO (1) | WO2013116698A2 (enExample) |
Families Citing this family (41)
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| US10533998B2 (en) | 2008-07-18 | 2020-01-14 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
| EP3913375A1 (en) | 2006-01-11 | 2021-11-24 | Bio-Rad Laboratories, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| US9562837B2 (en) | 2006-05-11 | 2017-02-07 | Raindance Technologies, Inc. | Systems for handling microfludic droplets |
| EP2021113A2 (en) | 2006-05-11 | 2009-02-11 | Raindance Technologies, Inc. | Microfluidic devices |
| US8772046B2 (en) | 2007-02-06 | 2014-07-08 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
| WO2008130623A1 (en) | 2007-04-19 | 2008-10-30 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
| US12038438B2 (en) | 2008-07-18 | 2024-07-16 | Bio-Rad Laboratories, Inc. | Enzyme quantification |
| WO2010009365A1 (en) | 2008-07-18 | 2010-01-21 | Raindance Technologies, Inc. | Droplet libraries |
| WO2010111231A1 (en) | 2009-03-23 | 2010-09-30 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
| US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
| EP4484577A3 (en) | 2010-02-12 | 2025-03-26 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| EP2622103B2 (en) | 2010-09-30 | 2022-11-16 | Bio-Rad Laboratories, Inc. | Sandwich assays in droplets |
| WO2012109600A2 (en) | 2011-02-11 | 2012-08-16 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
| US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
| US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
| EP3305918B1 (en) | 2012-03-05 | 2020-06-03 | President and Fellows of Harvard College | Methods for epigenetic sequencing |
| US8975042B2 (en) * | 2012-12-21 | 2015-03-10 | Dna Twopointo, Inc. | Fluorescent and colored proteins and methods for using them |
| US9290552B2 (en) | 2012-12-21 | 2016-03-22 | Dna Twopointo, Inc. | Fluorescent and colored proteins and methods for using them |
| US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
| US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| LT3456846T (lt) * | 2014-04-21 | 2022-09-12 | President And Fellows Of Harvard College | Nukleorūgšties unikalios sekos įvedimo sistemos ir būdai |
| EP3268462B1 (en) * | 2015-03-11 | 2021-08-11 | The Broad Institute, Inc. | Genotype and phenotype coupling |
| EP3283629A4 (en) | 2015-04-17 | 2018-08-29 | President and Fellows of Harvard College | Barcoding systems and methods for gene sequencing and other applications |
| WO2016187466A1 (en) * | 2015-05-20 | 2016-11-24 | Invenra, Inc. | Methods, apparatus and compositions for expression and high throughput screening |
| WO2017004250A1 (en) | 2015-06-29 | 2017-01-05 | Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University | Systems and methods for continuous flow digital droplet polymerase chain reaction bioanalysis |
| US11965891B2 (en) | 2015-12-30 | 2024-04-23 | Bio-Rad Laboratories, Inc. | Digital protein quantification |
| JP7086856B2 (ja) * | 2016-04-12 | 2022-06-20 | メディミューン，エルエルシー | 免疫レパートリー発掘 |
| KR102657960B1 (ko) * | 2016-10-18 | 2024-04-16 | 삼성전자주식회사 | 힘 전달 프레임 및 이를 포함하는 운동 보조 장치 |
| JP2020500517A (ja) | 2016-11-28 | 2020-01-16 | アリゾナ ボード オブ リージェンツ オン ビハーフ オブ アリゾナ ステート ユニバーシティ | 連続流小滴反応に関連するシステムおよび方法 |
| MY201744A (en) | 2016-12-23 | 2024-03-15 | Visterra Inc | Binding polypeptides and methods of making the same |
| GB2577214B (en) * | 2017-05-05 | 2021-10-06 | Scipio Bioscience | Methods for trapping and barcoding discrete biological units in hydrogel |
| CN118599963A (zh) | 2017-09-22 | 2024-09-06 | 华盛顿大学 | 细胞分子的原位组合标记 |
| WO2019161243A1 (en) * | 2018-02-15 | 2019-08-22 | Enevolv, Inc. | Sensor systems |
| WO2019195197A1 (en) | 2018-04-02 | 2019-10-10 | Dropworks, Inc. | Systems and methods for serial flow emulsion processes |
| WO2020193368A1 (en) * | 2019-03-22 | 2020-10-01 | Qiagen Gmbh | Method for improving the amplification efficiency of bead-based emulsion pcr (empcr) |
| WO2020247976A1 (en) * | 2019-06-03 | 2020-12-10 | Dropworks, Inc. | Systems and methods for stabilizing emulsions |
| CN114829626A (zh) | 2019-10-10 | 2022-07-29 | 1859公司 | 用于微流体筛选的方法和系统 |
| EP4189085A4 (en) * | 2020-07-28 | 2024-08-28 | Protillion Biosciences, Inc. | SYSTEMS AND METHODS FOR TESTING MULTIPLE POLYPEPTIDES |
| EP4200434A4 (en) * | 2020-08-21 | 2024-10-02 | Hifibio (Shanghai) Co., Ltd. | FUNCTIONAL SCREENING USING DROPLET MICROFLUIDIC |
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-
2013
- 2013-02-01 EP EP13706121.4A patent/EP2809783A2/en not_active Withdrawn
- 2013-02-01 CA CA2861387A patent/CA2861387A1/en not_active Abandoned
- 2013-02-01 WO PCT/US2013/024406 patent/WO2013116698A2/en not_active Ceased
- 2013-02-01 JP JP2014555774A patent/JP6196241B2/ja not_active Expired - Fee Related
- 2013-02-01 US US14/375,943 patent/US9701959B2/en not_active Expired - Fee Related
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