JP6151027B2 - Ceramide production promoter - Google Patents

Description

  The present invention relates to a ceramide production promoter and a humectant.

Ceramide, one of the sphingolipids, is a trace amount in the whole organism, but in the stratum corneum, the outermost layer of the skin, occupies more than half of the lipid component and is important for the skin moisturizing and barrier mechanisms Plays an important role. Ceramide builds a lamellar structure between cells in the stratum corneum after being produced and secreted in epidermal cells. In skin diseases such as dry skin, rough skin, atopic dermatitis, senile psoriasis and psoriasis, the healthy metabolism of ceramide is impeded, and the amount of ceramide in the stratum corneum is reduced, so that the skin's moisturizing ability and barrier ability It has been reported a lot that causes the decrease of
If ceramide production in epidermal cells is enhanced and the amount of ceramide in the stratum corneum can be increased, the skin's moisturizing ability and barrier ability can be increased, and skin diseases associated with a decrease in the amount of ceramide in the stratum corneum, such as dryness It is considered useful for the prevention and improvement of skin, rough skin, atopic dermatitis, senile psoriasis, psoriasis and the like. For example, eucalyptus extract is known to enhance skin moisturizing ability and barrier ability by increasing ceramide production in epidermal cells and increasing the amount of ceramide in the stratum corneum (Non-patent Document 1). Up to now, attempts have been made to replenish the reduced ceramide from the outside, but the effect is not necessarily sufficient at present.

International Journal of Cosmetic Science, 2012, 34, 17-22.

  An object of the present invention is to provide a ceramide production promoter having a high ceramide production promotion effect. Moreover, this invention makes it a subject to provide the moisturizer excellent in the effect | action which accelerates | stimulates ceramide production.

In view of the above problems, the present inventors have conducted intensive studies. As a result, Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume), and Anmi of (Ammi visnaga) Plants The inventors have found that the extract has a high ceramide production promoting action, and obtained the knowledge that these are useful as novel moisturizing ingredients. The present invention has been completed based on this finding.

That is, the present invention is Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume), and Anmi (Ammi visnaga Ceramide production promoter containing an extract of at least one plant selected from the group consisting of) as an active ingredient.
The present invention also Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume), and Anmi (Ammi visnaga ) Moisturizer containing an extract of at least one plant selected from the group consisting of

  ADVANTAGE OF THE INVENTION According to this invention, the ceramide production promoter which has a high ceramide production promotion effect can be provided. Moreover, according to this invention, the moisturizing agent excellent in the ceramide production promotion effect can be provided.

Ceramide production promoter and humectants of the present invention, Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume) And an extract of at least one plant selected from the group consisting of Ammi visnaga as an active ingredient. As demonstrated in the examples described later, these extracts exhibit a high ceramide production promoting effect.

Jingyou (Gentiana macrophylla Pall.) Is a plant of the gentian family (Gentianaceae) gentian genus (Gentiana), it is also referred to as Oba gentian. It is known that a herbal medicine (shinkyu), which uses her roots, has diuretic, analgesic and anti-inflammatory effects.
Tanchikuyou (Lophatherum gracile Brongn) is a plant of the grass family (Poaceae) Sasakusa genus (Lophatherum), also referred to as a Sasakusa. It is known that the light bamboo leaves, which are herbal medicines using the leaves, have an antipyretic action and the like.
Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf) is a plant of the grass family (Poaceae) Phyllostachys (Phyllostachys), black bamboo, also referred to as an irresistible force. It is known that bamboo candy, which is a crude drug using this, has diuresis, analgesia, and anti-inflammation.
Goshitsu ( Achyranthes bidentata Blume ) is a plant of the Amaranthaceae family and is also called Hinata Kozuchi. It is known that bovine knee, which is a crude drug using this, has diuretic and blood purification effects.
Ammi visnaga is a plant belonging to the genus Apiaceae and Ammi . The fruits are used as crude drugs and are known to have diuretic effects and the like.

The extract used in the present invention is a whole tree, whole plant, or any part of the above plant (root, rhizome, trunk, branch, stem, leaf, bark, sap, resin, flower, fruit, seed, fruit peel, persimmon, Buds, flower spikes, heartwood, etc.). Moreover, you may use combining each site | part in multiple numbers. Moreover, you may use the crude drug obtained by using these plants as a base plant as an extraction raw material.
Preferably, the following sites are used for extraction.
In order to obtain an extract of gentian, it is preferable to use the root of the plant.
In order to obtain an extract of tanchikuyo, it is preferable to use whole plants or leaves of the plant.
In order to obtain the extract of chikujo, it is preferable to use the stem (crab) of the said plant, and it is more preferable to use the intermediate | middle layer (inner layer of straw) of the stem.
In order to obtain an extract of gourd, it is preferable to use the root of the plant.
In order to obtain an extract of Ammi, it is preferable to use the fruit of the plant.
The above plants may be used as they are in the raw state, and it is also preferable to use those subjected to treatments such as drying, shredding, and pulverization in order to increase extraction efficiency.

  There is no particular limitation on the method for obtaining the extract from the plants of gentian, red pepper, chikujo, gosh and ammi, and the extract can be obtained by extracting the above plant by a usual method. Specifically, dried products obtained by drying the above plants, squeezed juice obtained by squeezing and extracting the pulverized products, steam distillates, crude extracts using various extraction solvents, distribution of crude extracts, column chromatography, etc. Extract fractions obtained by purification by various chromatographic methods can be used as the extract in the present invention. Moreover, you may use what combined these extract, steam distillate, pressing material, etc. individually or in combination of 2 or more types as an extract.

In the present invention, it is more preferable to use an extract obtained by using an extraction solvent from a dried product obtained by drying the plant or a pulverized product thereof.
As the extraction solvent, either a polar solvent or a nonpolar solvent can be used, and these can also be mixed and used. Examples of the extraction solvent include water; alcohol (for example, monohydric alcohols such as methanol, ethanol, propanol, and butanol, polyhydric alcohols such as ethylene glycol, propylene glycol, and butylene glycol); ketones such as acetone and methyl ethyl ketone; methyl acetate Esters such as ethyl acetate; Chain and cyclic ethers such as tetrahydrofuran and diethyl ether; Polyethers such as polyethylene glycol; Halogenated hydrocarbons such as dichloromethane, chloroform and carbon tetrachloride; Hydrocarbons such as hexane, cyclohexane and petroleum ether Aromatic hydrocarbons such as benzene and toluene; pyridine; supercritical carbon dioxide; fats and oils, waxes, and other oils. A mixture in which two or more of the above solvents are combined can also be used as the extraction solvent. Among them, it is preferable to use at least one selected from water, an alcohol, and an aqueous solution of the alcohol, more preferably at least one selected from water, an aqueous ethanol solution, propylene glycol, and butylene glycol. Alternatively, it is more preferable to use an ethanol aqueous solution.

The concentration of the aqueous alcohol solution used as the extraction solvent is preferably 0.001% by volume or more, more preferably 5% by volume or more, and still more preferably 40% by volume or more. The concentration is preferably 99.999% by volume or less, more preferably 99% by volume or less, and still more preferably 95% by volume or less. Furthermore, 0.001 volume% or more and 99.999 volume% or less are preferable, 5 volume% or more and 99 volume% or less are more preferable, and 40 volume% or more and 95 volume% or less are more preferable.
In particular, the concentration of the aqueous ethanol solution used as the extraction solvent is preferably 5% by volume or more, more preferably 40% by volume or more, preferably 99% by volume or less, and more preferably 95% by volume or less. Furthermore, 5 volume% or more and 99 volume% or less are preferable, and 40 volume% or more and 95 volume% or less are more preferable.

  The extraction conditions differ depending on the solvent to be used and are not particularly limited. For example, when extraction is performed using water, alcohol or an aqueous alcohol solution, it is preferably immersed or heated to reflux under the following conditions. The solvent is preferably used in an amount of 1 part by mass or more, preferably 50 parts by mass or less, based on 1 part by mass of the plant. The extraction temperature is preferably 3 ° C. or higher, more preferably 20 ° C. or higher, preferably 100 ° C. or lower, and more preferably 80 ° C. or lower. The extraction time is preferably 1 hour or longer, more preferably 1 day or longer, several weeks or shorter, more preferably 30 days or shorter. Moreover, in order to raise extraction efficiency, you may stir together or you may perform a homogenization process in a solvent.

The extract obtained by extraction with the above solvent may be used as it is, but it is also possible to fractionate and use a fraction having high activity by an appropriate separation means such as gel filtration, chromatography, precision distillation or the like. it can. The plant extract used in the present invention includes the various extracts thus obtained, a diluted solution thereof, a concentrated solution thereof, a purified product thereof or a dry powder thereof.
For the ceramide production promoter or moisturizing agent of the present invention, plant extracts of gentian, red pepper, chikujo, goshitsu and ammi may be used alone or in admixture of two or more.

As shown in the below-mentioned Examples, plant extracts obtained from gentian, licorice, chikujo, gosh and ammi have an excellent ceramide production promoting action. As described above, ceramide plays an important role in the skin moisturizing mechanism and barrier mechanism, and promotion of ceramide production normalizes the ceramide production mechanism of the living body, increases ceramide in the reduced stratum corneum, It leads to regaining skin having high barrier function and moisturizing function. In other words, plant extracts obtained from gentian, red snapper, chikujo, gosh and ammi can be used to promote ceramide production and thus maintain, improve and normalize the skin's moisturizing function and barrier function. it can.
The use may be a therapeutic use (ie medical practice) or a non-therapeutic use (non-medical practice). Moreover, the subject of the use can be a human, a non-human animal, or a specimen derived therefrom. The “non-therapeutic” is a concept that does not include a medical action, that is, a treatment action on the human body by treatment.

A ceramide production promoter or moisturizer containing a plant extract of gentian, red pepper, chikujo, gothitsu, or ammi as an active ingredient is one of the specific embodiments of the above-mentioned use, and has therapeutic use (medical use), non-use It can be applied to any therapeutic use (non-medical use). The usage forms include all forms that can be applied to human, animal skin, nails, mucous membranes, hair and the like.
Specific examples include use as pharmaceuticals, quasi-drugs, cosmetics, etc., in the case of cosmetics, quasi-drugs, and pharmaceuticals, in the form of external skin preparations, and in the case of pharmaceuticals, in the dosage form can do. Moreover, these agents can also be mix | blended as an active ingredient in various food-drinks, pet food, etc.

The ceramide production promoter or moisturizing agent of the present invention may contain only the above-mentioned plant extract that is an active ingredient, or it contains other ingredients within a range that does not affect the effect. May be.
Components other than the active ingredient can be appropriately selected according to the form and use of the agent. For example, an appropriate liquid or solid excipient or extender such as titanium oxide, calcium carbonate, distilled water, lactose, starch or the like may be added so as to facilitate forming as an agent. In addition, other medicinal components (other whitening agents, moisturizers, etc.), antioxidants, ultraviolet absorbers, surfactants, thickeners, colorant types, and the like may be added.
Examples of other medicinal ingredients include humectants such as known ceramide production promoters, pseudoceramides, natural ceramides, and sugar ceramides. Examples of medicinal ingredients other than the humectant include skin aging inhibitors and whitening agents.
Known ceramide production promoters include, for example, acetylhydroxyproline, potassium glycyrrhizinate, L-carnitine, ascorbic acid, ascorbyl glucoside, magnesium ascorbyl phosphate, dl-α-tocopheryl-dl-ascorbyl phosphate, dl-α- Tocopheryl phosphate, nicotinamide, tocopherol nicotinate, L-lactic acid, vitamin C, asparagus extract, butcher bloom, genquanine, rosemary, lavender, sage, jujube, black (red) ganoderma, toki, kujin, Yokuinin, Beniceanne extract, rice power extract and the like.
Examples of the pseudo ceramide include commercially available ceramide R (manufactured by Unilever), ceramide PC-104 (manufactured by Taiheiyo Kagaku), ceramide HO3 (manufactured by sederma), and Erdew PS-203 (manufactured by Ajinomoto).
Examples of sugar ceramides include glucosyl ceramide, galactosyl ceramide, etc., and commercially available products include nippon ceramide (manufactured by Nippon Flour), oryzah ceramide (manufactured by Oriza Yoka), Nissan ceramide, neo-liquid ceramide N (Japanese fat and oil Manufactured), ceramide (manufactured by Unitika) and the like.

  When other ingredients are added to the agent, the content of the plant extract, which is an active ingredient, is not particularly limited, but the extract is 0.00001% by mass or more in terms of solid content concentration (in terms of solid content) in the agent. It is preferably contained, and more preferably 0.0001% by mass or more. Moreover, it is preferable that 20 mass% or less of the said extract is contained by solid content concentration in an agent, and it is more preferable that 10 mass% or less is contained. Furthermore, it is preferable that the said extract is 0.00001 mass% or more and 20 mass% or less by solid content concentration in an agent, and it is more preferable that 0.0001 mass% or more and 10 mass% or less are contained.

When the ceramide production promoter or moisturizer of the present invention is used in the form of a skin external preparation, the “skin external preparation” is applied to the skin as a skin cosmetic, an external medicine, an external quasi-drug, etc. The dosage form can take a wide variety of forms such as aqueous solution, solubilization system, emulsification system, powder system, gel system, ointment system, cream, water-oil two-layer system, water-oil-powder three-layer system. obtain. More specifically, facial cleanser, lotion, lotion, milky lotion, cream, gel, essence (beauty serum), pack, mask, massage agent, foundation, lipstick, bath preparation, shampoo, hair conditioner, hair tonic, ointment, Examples include pastes, sheet-like products (absorbent articles such as sanitary products, wiping wipes, wet tissues, etc.).
In addition, in making these dosage forms, various oil agents, surfactants, gelling agents, preservatives, antioxidants, solvents, alcohol, water, chelating agents, thickeners, ultraviolet absorbers, emulsion stabilizers, pH A regulator, a pigment, a fragrance, and the like can be appropriately blended.

When the ceramide production promoter or moisturizer of the present invention is used as a pharmaceutical in the form of administration to humans or animals, for example, oral solid preparations such as tablets, coated tablets, granules, powders, capsules, oral liquids, syrups It can be in the form of oral liquid preparations such as pills, parenteral preparations such as injections, external preparations, suppositories, and transdermal absorption agents.
In preparing these preparations, excipients, binders, extenders, disintegrants, surfactants, lubricants, dispersants, buffers, preservatives, flavoring agents, fragrances, coating agents, carriers, Diluents and the like can be appropriately blended.

When the ceramide production promoter or moisturizing agent of the present invention is blended as an active ingredient in foods and drinks, pet foods, etc., in addition to general foods and drinks, it is based on the concept of improving and maintaining skin moisturizing ability or barrier function, if necessary It can be added to and blended with functional foods and beverages such as health foods and beverages, beauty foods and beverages, health foods and beverages for specified health use, foods and beverages for the sick, and the like.
There is no particular limitation on the type of food and drink, for example, beverages such as fruit juice beverages, milk beverages, tea-based beverages, confectionery such as candy, drop, jelly, cookies, chocolate, cakes, yogurt, gum, seasonings, Examples include cooking oil, dairy products, breads, noodles, and processed rice. Moreover, it is good also as beauty foods, health foods, etc., such as a tablet (tablet), a capsule, a granule, and a syrup.

  The daily use amount of the ceramide production promoter or moisturizing agent of the present invention per adult varies depending on the content of the active ingredient in the agent, the condition of the application subject, body weight, sex, age, etc. The plant extract is preferably used in an amount of 0.001 mg or more, more preferably 0.01 mg or more in terms of dry solid content. The plant extract is preferably used in an amount of 1000 mg or less, more preferably 100 mg or less in terms of dry solid content. Further, the plant extract is preferably used in an amount of 0.001 mg to 1000 mg, more preferably 0.01 mg to 100 mg in terms of dry solid content.

  The ceramide production promoter and moisturizer of the present invention can enhance ceramide production in epidermal cells and increase the amount of ceramide in the stratum corneum, thereby enhancing the skin moisturizing function and barrier function. Due to this action, the ceramide production promoter and moisturizer of the present invention can prevent skin diseases caused by a decrease in the amount of ceramide in the stratum corneum, such as dry skin, rough skin, atopic dermatitis, senile psoriasis and psoriasis. Can prevent, mitigate, improve. From this point of view, the ceramide production promoter and moisturizer of the present invention are skin barrier function improver, dry skin preventive agent or improver, rough skin preventive agent or improver, atopic dermatitis preventive agent or improver, senile It can be used as an agent for preventing or improving psoriasis, an agent for preventing or improving psoriasis, and the like.

  The present invention further discloses the following ceramide production promoter, humectant, method and application regarding the above-described embodiment.

<1> Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), consisting Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume), and Anmi (Ammi visnaga) A ceramide production promoter or moisturizer containing an extract of at least one plant selected from the group as an active ingredient.

<2> The ceramide production promoter or moisturizer according to <1>, wherein the content of the plant extract as an active ingredient is 0.00001% by mass or more in terms of solid content, and preferably 0.0001% by mass or more. Agent.
<3> The ceramide production promoter or moisturizer according to <1> or <2>, wherein the content of the plant extract as an active ingredient is 20% by mass or less in terms of solid content, and preferably 10% by mass or less. Agent.
<4> The content of the plant extract as an active ingredient is 0.00001% by mass to 20% by mass in terms of solid content, and preferably 0.0001% by mass to 10% by mass, <1> to <3> The ceramide production promoter or humectant according to any one of the above.

<5> The extract according to any one of <1> to <4>, wherein the extract is an extract obtained by extracting the plant with at least one solvent selected from water, alcohol, and an aqueous solution of the alcohol. 2. The ceramide production promoter or humectant according to item 1.
<6> The ceramide production promoter or humectant according to <5>, wherein the solvent is at least one selected from water, an aqueous ethanol solution, propylene glycol, and butylene glycol.
<7> The ceramide production promoter or humectant according to <5> or <6>, wherein the solvent is water or an aqueous ethanol solution.
<8> The concentration of the aqueous alcohol solution is 0.001% by volume or more, preferably 5% by volume or more, and more preferably 40% by volume or more, according to any one of <5> to <7>. Ceramide production promoter or moisturizer.
<9> The concentration of the aqueous alcohol solution is 99.999% by volume or less, preferably 99% by volume or less, and more preferably 95% by volume or less, according to any one of <5> to <8>. Ceramide production promoter or moisturizer.
<10> The concentration of the aqueous alcohol solution is 0.001% by volume or more and 99.999% by volume or less, more preferably 5% by volume or more and 99% by volume or less, and further preferably 40% by volume or more and 95% by volume or less. The ceramide production promoter or humectant according to any one of <5> to <9>.
<11> The concentration of the aqueous ethanol solution is 5% by volume or more, preferably 40% by volume or more, 99% by volume or less, and preferably 95% by volume or less, any one of <6> to <10> 2. The ceramide production promoter or humectant according to item 1.
<12> The ceramide production according to any one of <6> to <11>, wherein the concentration of the aqueous ethanol solution is 5% by volume to 99% by volume and preferably 40% by volume to 95% by volume. Accelerator or humectant.
<13> The extract uses the solvent in an amount of 1 part by mass or more and 50 parts by mass or less based on 1 part by mass of the plant, 3 ° C or higher (preferably 20 ° C or higher), 100 ° C or lower (preferably 80 ° C or lower). Any one of <5> to <12>, which is an extract obtained by immersion or extraction under heating for 1 hour or more (preferably 1 day or more) and several weeks or less (preferably 30 days or less). The ceramide production promoter or humectant according to Item.

<14> The ceramide production promoter or humectant according to any one of <1> to <13>, which is provided for external use on the skin.
<15> The extract is at least one selected from the group consisting of Gentian Root Extract, Tanchikuyo Whole or Leaf Extract, Chikujo Stalk Extract, Goshitsu Root Extract, and Amimi Fruit Extract. The ceramide production promoter or humectant according to any one of <1> to <14>, wherein

<16> Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), consisting Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume), and Anmi (Ammi visnaga) A method of promoting ceramide production (excluding medical practice) or a skin moisturizing method (excluding medical practice), characterized in that a cosmetic comprising at least one plant extract selected from the group is applied to the skin.
<17> Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), consisting Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume), and Anmi (Ammi visnaga) A method of promoting ceramide production (excluding medical practice) or a method of moisturizing skin (excluding medical practice) characterized by orally ingesting a food or drink containing an extract of at least one plant selected from the group .

<18> Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), consisting Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume), and Anmi (Ammi visnaga) A method of using an extract of at least one plant selected from the group as a ceramide production promoter or humectant.
<19> as a ceramide production promoter or moisturizing agents, Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume ) And at least one plant extract selected from the group consisting of Ammi visnaga .
<20> for ceramide production-accelerating or moisturizing, Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume) And non-pharmaceutical (non-therapeutic) use of an extract of at least one plant selected from the group consisting of Ammi visnaga .
<21> for the preparation of a ceramide production-accelerating agent or humectant, Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu ( Use of an extract of at least one plant selected from the group consisting of Achyranthes bidentata Blume ) and Ammi visnaga .
<22> used for ceramide production-accelerating or moisturizing, Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume ), And an extract of at least one plant selected from the group consisting of Ammi visnaga .
<23> Jingyou (Gentiana macrophylla Pall.), Tanchikuyou (Lophatherum gracile Brongn), consisting Chikujo (Phyllostachys nigra (Loddiges) Munro var . Henonis (Bean) Stapf), Goshitsu (Achyranthes bidentata Blume), and Anmi (Ammi visnaga) A method for promoting ceramide production or a moisturizing method, comprising administering or applying to the skin at least one plant extract selected from the group.

<24> The content of the plant extract in the ceramide production promoter or moisturizer is 0.00001% by mass or more in terms of solid content, and preferably 0.0001% by mass or more, <18>, < The method or use according to any one of 19> and <21>.
<25> The method or use according to <24>, wherein the content of the plant extract in the ceramide production promoter or moisturizer is 20% by mass or less in terms of solid content, and preferably 10% by mass or less. .
<26> The content of the plant extract in the ceramide production promoter or moisturizer is 0.00001% by mass to 20% by mass in terms of solid content, and is 0.0001% by mass to 10% by mass. Or a method or use according to <24> or <25>.

<27> The extract according to any one of <16> to <25>, wherein the extract is an extract obtained by extracting the plant with at least one solvent selected from water, alcohol, and an aqueous solution of the alcohol. 2. Extract, method or use according to 1.
<28> The extract, method or use according to <27>, wherein the solvent is at least one selected from water, an aqueous ethanol solution, propylene glycol, and butylene glycol.
<29> The extract, method or use according to <27> or <28>, wherein the solvent is water or an aqueous ethanol solution.
<30> The extract, method or use according to any one of <27> to <29>, wherein the concentration of the aqueous alcohol solution is 0.001% by volume or more and preferably 5% by volume or more.
<31> The extract, method or use according to any one of <27> to <30>, wherein the concentration of the aqueous alcohol solution is 99.999% by volume or less and preferably 95% by volume or less.
<32> The concentration of the aqueous alcohol solution is 0.001 to 99.999% by volume, preferably 5 to 95% by volume, and any one of <27> to <31> The described extract, method or use.
<33> The concentration of the aqueous ethanol solution is 5% by volume or more, preferably 40% by volume or more, 99% by volume or less, and preferably 95% by volume or less, any one of <28> to <32> 2. Extract, method or use according to 1.
<34> The extract according to any one of <28> to <33>, wherein the concentration of the aqueous ethanol solution is 5% to 99% by volume, and preferably 40% to 95% by volume. , Method or use.
<35> The extract uses the solvent in an amount of 1 part by mass or more and 50 parts by mass or less based on 1 part by mass of the plant, 3 ° C or higher (preferably 20 ° C or higher), 100 ° C or lower (preferably 80 ° C or lower). Any one of <27> to <34>, which is an extract obtained by immersion or extraction under heating for 1 hour or more (preferably 1 day or more) and several weeks or less (preferably 30 days or less). The extract, method or use according to paragraph.

<36> The extract, method or use according to any one of <16> to <35>, which is provided for external use on the skin.
<37> The extract is at least one selected from the group consisting of Gentian Root Extract, Tanchikuyo Whole or Leaf Extract, Chikujo Stalk Extract, Goshitsu Root Extract, and Amimi Fruit Extract. The extract, method or use according to any one of <16> to <36>.

  EXAMPLES Hereinafter, although this invention is demonstrated further in detail based on an Example, this invention is not limited to this.

(Manufacture example 1) Preparation of the water extract of gentian A 40 g root of gentian (made by Shinwa Bussan Co., Ltd.) was shredded, 400 mL of water was added, and extraction was performed at 70 ° C. for 5 hours. Thereafter, the mixture was filtered to obtain 246 g of a water extract of gentian. About the obtained extract, when the evaporation residue was computed by the following method, the evaporation residue was 4.81% (w / v). This was concentrated to dryness and then diluted with a 10% aqueous ethanol solution to prepare a 1.0% (w / v) (extract solid content) extract.
<Calculation of evaporation residue>
When 1 mL of gentian water extract was dried at 105 ° C. for 6 hours (dryer: DRY Thermo Unit DTU-1C (manufactured by TAITEC CORPORATION) was used), 48.1 mg of a dried product was obtained. The evaporation residue of this extract was calculated as 48.1 / 1000 × 100 = 4.81% (w / v). In the following production examples, the evaporation residue of each extract was calculated in the same manner.

(Manufacturing example 2) Preparation of 50% ethanol extract of Genghyo Shred 40 g of root of Genghyo (manufactured by Shinwa Bussan Co., Ltd.), add 400 mL of 50% ethanol aqueous solution, and extract for 7 days under room temperature and standing conditions. went. Thereafter, filtration was performed to obtain 306 g of a 50% ethanol aqueous solution extract of gentian. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 4.74% (w / v). This was diluted with 50% aqueous ethanol solution to prepare 1.0% (w / v) (extract solid content) extract.

(Production Example 3) Preparation of 95% ethanol extract of Genghyo 40 g of root of Genghyo (manufactured by Shinwa Bussan Co., Ltd.), add 400 mL of 95% ethanol aqueous solution, and extract for 7 days under room temperature and standing conditions went. Thereafter, filtration was performed to obtain 287 g of a 95% ethanol aqueous solution extract of gentian. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 1.64% (w / v). This was diluted with 95% aqueous ethanol solution to prepare a 1.0% (w / v) (extract solid content) extract.

(Manufacture example 4) Preparation of water extract of tanchiyo 40 leaf of tanchiyo (manufactured by Shinwa Bussan Co., Ltd.) was shredded, 400 mL of water was added, and extraction was performed at 70 ° C. for 5 hours. Thereafter, filtration was performed to obtain 226 g of a water extract of tanchiyo. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 0.95% (w / v). This was concentrated to dryness and then diluted with a 10% aqueous ethanol solution to prepare a 1.0% (w / v) (extract solid content) extract.

(Production Example 5) Preparation of 95% ethanol extract of Tanchikuyo Shred 40 g of leaves of Tanchikuyo (manufactured by Shinwa Bussan Co., Ltd.), add 400 mL of 95% ethanol aqueous solution, and extract for 7 days under room temperature and standing conditions. went. Thereafter, filtration was performed to obtain 215 g of a 95% ethanol aqueous extract of tanchikuyo. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 0.35% (w / v). This was concentrated to dryness and then diluted with 95% aqueous ethanol solution to prepare a 1.0% (w / v) (extract solid content) extract.

(Production Example 6) Preparation of chikujo water extract 40 g of chikujo (manufactured by Shinwa Bussan Co., Ltd.) stem intermediate layer (inner layer of cocoon) was added, 400 mL of water was added, and extraction was performed at 70 ° C. for 5 hours. It was. Thereafter, filtration was performed to obtain 300 g of an aqueous extract of Chikujo. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 0.95% (w / v). This was concentrated to dryness and then diluted with a 10% aqueous ethanol solution to prepare a 1.0% (w / v) (extract solid content) extract.

(Production Example 7) Preparation of 50% ethanol extract of chikujo 40g of the middle layer of the stem of Chikujo (manufactured by Shinwa Bussan Co., Ltd.) and 400ml of 50% aqueous ethanol solution were added. Extraction was carried out for 7 days under storage conditions. Thereafter, filtration was performed to obtain 279 g of a 50% ethanol aqueous solution extract of Chikujo. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 0.98% (w / v). This was concentrated to dryness and then diluted with 50% aqueous ethanol solution to prepare a 1.0% (w / v) (extract solid content) extract.

(Manufacture example 8) Preparation of the water extract of goshtsu 40g of roots of goshtsu (made by Shinwa Bussan Co., Ltd.) were shredded, 400mL of water was added, and extraction was performed at 70 ° C for 5 hours. Thereafter, filtration was performed to obtain 325 g of an aqueous extract of gourds. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 6.05% (w / v). This was concentrated to dryness and then diluted with a 10% aqueous ethanol solution to prepare a 1.0% (w / v) (extract solid content) extract.

(Manufacture example 9) Preparation of 50% ethanol extract of gourd Shred 40g of root of gosh (manufactured by Shinwa Bussan Co., Ltd.), add 400mL of 50% aqueous ethanol solution, and extract for 7 days under room temperature and standing conditions. went. Thereafter, filtration was performed to obtain 301 g of a 50% ethanol aqueous solution extract of Gosh. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 5.97 (w / v). This was diluted with 50% aqueous ethanol solution to prepare 1.0% (w / v) (extract solid content) extract.

(Manufacture example 10) Preparation of 95% ethanol extract of gourd 40g root of gosh (manufactured by Shinwa Bussan Co., Ltd.) is shredded, 400mL of 95% ethanol aqueous solution is added, and extraction is performed for 7 days under room temperature and standing conditions. went. Thereafter, filtration was performed to obtain 303 g of a 95% aqueous ethanol extract of Gothic. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 0.18% (w / v). This was concentrated to dryness and then diluted with 95% aqueous ethanol solution to prepare a 1.0% (w / v) (extract solid content) extract.

(Production Example 11) Preparation of Ammi's water extract 50 g of Ammi's (Monteagle Herbs) fruit was shredded, 500 mL of water was added, and extraction was performed at 70 ° C for 5 hours. Then, it filtered and the water extract of Ammi was obtained. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 6.95% (w / v). This was concentrated to dryness and then diluted with a 10% aqueous ethanol solution to prepare a 1.0% (w / v) (extract solid content) extract.

(Production Example 12) Preparation of 50% ethanol extract of Ammi 50 g of Ammi (Monteagle Herbs) fruit was chopped, 500 mL of 50% ethanol aqueous solution was added, and extraction was performed for 15 days under room temperature and standing conditions. . Thereafter, filtration was performed to obtain a 50% aqueous ethanol extract of Ammi. When the evaporation residue was calculated for the obtained extract, the evaporation residue was 4.64% (w / v). This was diluted with 50% aqueous ethanol solution to prepare 1.0% (w / v) (extract solid content) extract.

(Test example) Verification of ceramide production promoting effect Normal human epidermal keratinocytes (trade name: NHEK (F), KURABO) in culture solution (trade name: EpiLife-KG2, manufactured by KURABO) using a culture plate Manufactured at 37 ° C. and 5% CO ₂ .
Thereafter, the culture solution was replaced with EpiLife-KG2 from which growth factors such as epidermal growth factor were removed, and an extract with a concentration of 1.0 w / v% (in terms of solid content) prepared in the above production example, or 50% ethanol as a control solution. Was added in an amount of 0.1% (final concentration of the extract: 0.001 (w / v)%).
After culturing for 3 days, each cell was collected together with 1 well.

The organic phase from which the lipid was extracted from the collected cells by the Bligh and Dyer method was transferred to a glass tube, solidified with nitrogen, and then redissolved with chloroform and methanol to obtain a lipid sample.
In addition, 0.1N NaOH, 1% SDS aqueous solution is added to the cells after lipid extraction, and the protein is solubilized by heating at 60 ° C. for 2 hours, cooled to room temperature, and then neutralized by adding 2N HCl. The amount of protein was quantified by the BCA method.

The prepared lipid sample was horizontally developed by thin film chromatography (TLC) twice with chloroform: methanol: acetic acid = 190: 9: 1. The copper sulfate solution was sprayed and sprayed with a hot plate to detect ceramide, and the amount was determined.
The results are shown in Table 1. In addition, the amount of ceramide in Table 1 shows a relative value (average value of n = 3) when the amount of ceramide in the system to which the control solution is added is 1.

(Reference example)
1. Preparation of Eucalyptus Extract 40 g of Eucalyptus globulus Labillardiere (purchased from Shinwa Bussan Co., Ltd.) was cut into small pieces, added with 400 mL of 50% ethanol, and extracted for 7 days at room temperature and standing conditions. Then, it filtered and 291 mL of eucalyptus extracts were obtained. When the evaporation residue was calculated by the above method for the obtained extract, the evaporation residue was 3.16% (w / v).

2. Verification of ceramide production promoting effect Using the obtained eucalyptus extract, the ceramide production promoting effect was verified by the same method as in the above test example. In addition, 0.001% of the extract adjusted so that a density | concentration might be 1% (w / v) in conversion of solid content added to the cell.
The results are shown in Table 1. The eucalyptus extract is known as a moisturizing component having a ceramide production promoting action as described above.

  As is apparent from Table 1, the amount of ceramide was increased in the system to which extracts of gentian, red pepper, chikujo, gossits and ammi were added compared to the control system. That is, it was found that these plant extracts have an excellent ceramide production promoting action and are useful as ceramide production promoters. Furthermore, since these plant extracts have a ceramide production effect equivalent to that of a eucalyptus extract, which is a known moisturizing component, it has been found useful as a moisturizing agent.

(Prescription example)
Using the extract obtained in the above production example as an active ingredient, lotion having the following composition, 0 / W (oil-in-water) type emulsion, W / O (water-in-oil) type cream, gel cosmetic, liquid Bathing agents can be produced by conventional methods.

1. Preparation of lotion (composition) (formulation: mass%)
Gentian extract 0.01
Polyethylene glycol (trade name: PEG-1540, manufactured by Sanyo Chemical Co., Ltd.) 1.00
Polyoxyethylene (20) sorbitan monolaurate 1.50
Glycerin 2.00
Paraben 0.10
Purified water residue

2. Preparation of 0 / W emulsion (composition) (formulation: mass%)
Tanchikuyo extract 0.03
Polyethylene glycol (trade name: PEG-2000, manufactured by Sanyo Chemical Co., Ltd.) 1.00
Pullulan (trade name: Pullulan PT-20, manufactured by Hayashibara) 0.40
Cetyl alcohol 1.00
Vaseline 2.00
Squalane 6.00
Dimethylpolysiloxane 2.00
Glycerin 2.00
Pseudoceramide (N- (3-hexadecyloxy-2-hydroxypropyl) -N-2-hydroxyethylhexadecaminad) 1.00
Polyoxyethylene (10) monooleate 1.00
Glycerol monostearate 1.00
Acid heteropolysaccharide derived from plant callus (1% by weight aqueous solution of tuberose polysaccharide) 2.00
Paraben 0.20
Purified water residue

3. Preparation (composition) of W / O type cream (Formulation: mass%)
Chikujo extract 0.05
Alkyl acrylate copolymer (trade name: Yodosol GH810, manufactured by Kanebo NSC)
1.30
Polyvinylpyrrolidone (Brand name: Rubiscol K-90, manufactured by BASE Japan)
0.70
Dimethylpolysiloxane 10.00
Methylphenylpolysiloxane 3.00
Octamethylcyclotetrasiloxane 12.00
Polyoxyalkylene modified silicone 5.00
1,3-butylene glycol 6.00
Pseudoceramide (N- (3-hexadecyloxy-2-hydroxypropyl) -N-2-hydroxyethylhexadecaminad) 1.20
Paraben 0.20
Perfume Trace amount Purified water Residual

4). Preparation (composition) of gel cosmetic (formulation: mass%)
Goshitsu extract 0.01
Polyethylene glycol (trade name: PEG-2000, manufactured by Sanyo Chemical Co., Ltd.) 0.50
Xanthan gum (trade name: Neosoft XKK, manufactured by Kojinsha) 0.20
Glycerin 3.00
Ethanol 3.00
Carboxyvinyl polymer 0.50
Potassium hydroxide 0.15
Polyoxyethylene hydrogenated castor oil 1.00
Citric acid 0.80
Trisodium citrate 0.80
Nylon powder 1.00
Paraben 0.10
Perfume Trace amount Purified water Residual

5). Preparation of liquid bathing agent (composition) (formulation: mass%)
Ammi extract 0.02
Pseudoceramide (N- (3-hexadecyloxy-2-hydroxypropyl) -N-2-hydroxyethylhexadecaminad) 0.10
Isopropyl myristate 15.00
Polyoxyethylene (12) oleyl ether 10.00
Polyoxyethylene (6) oleyl ether 6.00
Acid heteropolysaccharide derived from plant callus (1% by weight aqueous solution of tuberose polysaccharide) 2.00
Paraben 0.30
Perfume Trace amount Liquid paraffin Residue

Claims (2)

Ohba gentian (Gentiana macrophylla Pall.) Ceramide production-accelerating agent containing as an active ingredient extract extract. Ohba gentian (Gentiana macrophylla Pall.) Moisturizing agent containing as an active ingredient extract extract.