KR20200050032A - Anti-inflammatory composition containing mixed extracts of fermented Gentianae scabrae Radix, Chrysanthemum indicum and Mentha piperascens - Google Patents

Abstract

The present invention relates to a pharmaceutical, health functional food, health food, and cosmetic composition for anti-inflammation containing a fermented Gentiana scabra extract, a Chrysanthemum morifolium extract, and a Mentha Canadensis extract. The composition according to the present invention contains a mixed extract of one or more extracts selected from a group consisting of the fermented Gentiana scabra extract, the Chrysanthemum morifolium extract, and the Mentha Canadensis extract, thereby having an effect of significantly improving an anti-inflammatory effect. In addition, the composition does not affect a cell survival rate to have no side effects, thereby being able to be usefully used for the natural pharmaceutical, health functional food, health food, and cosmetic composition for anti-inflammation.

Description

Anti-inflammatory composition containing mixed extracts of fermented Gentianae scabrae Radix, Chrysanthemum indicum and Mentha piperascens}

The present invention relates to an anti-inflammatory composition containing fermented gentian extract, chrysanthemum extract and mint extract, specifically, anti-inflammatory containing at least one extract selected from the group consisting of fermented gentian extract and chrysanthemum extract and mint extract. It relates to pharmaceutical, health functional food, health food and cosmetic composition.

Inflammation is a body's defense response to infection from external infectious agents such as external physical and chemical stimuli, bacteria, fungi, viruses, and various allergens. The inflammatory response is part of the innate immune response, and as in other animals, the innate immune response in humans begins by recognizing and attacking non-self through macrophage-specific patterns of cell surfaces present in pathogens. During an inflammatory reaction, plasma accumulates in the inflamed area, diluting the toxicity secreted by bacteria, increasing blood flow, and accompanying symptoms such as erythema, pain, edema, and fever.

Although various biochemical phenomena are involved in the inflammatory reaction, macrophages are known to play an important role in the inflammatory reaction by generating nitric oxide (NO) and various inflammatory cytokines by chemical stimulation. Nitric oxide is synthesized from L-arginine by the action of nitric oxide synthase (NOS), and NOS has several isoforms. BNOS (brain NOS) present in the brain, nNOS (neuronal NOS) present in the nervous system, and eNOS (endothelial NOS) present in the vascular endothelial system are always expressed at a certain level in the body, and a small amount of monoxide produced by them Nitrogen (NO) plays an important role in maintaining homeostasis by performing various physiological reactions related to blood pressure regulation, neurotransmission, learning, and memory. On the other hand, iNOS (induced NOS), whose expression is induced by a certain stimulus, generates excessive NO, and nitric oxide generated excessively by iNOS reacts with superoxide to form peroxynitrite. It acts as a powerful oxidant, damaging cells, thereby participating in various pathological processes, including inflammation and cancer.

Meanwhile, Gentiana scabra ) is a perennial plant belonging to Gentian and Gentian, distributed in Korea, China, and Japan. Oriental medicine (Gentianae scabrae Radix) is the root and rhizome of gentian, and has the effect of warm and humid with cold and cold properties.

Chrysanthemum ( Chrysanthemum indicum ) is a perennial plant of the Chrysanthemum family, distributed in Korea, China, and Japan. It is sweet, bitter and cool in oriental medicine, and has the effect of removing wind fever, which is used for inflammation such as boils.

Peppermint ( Mentha piperascens ) is a herbaceous and peppermint plant distributed worldwide. In oriental medicine, it is a spicy and cool property and is used for various diseases caused by wind fever.

However, the anti-inflammatory efficacy of the mixture of all of the gentian, chrysanthemum and peppermint has not been studied. Accordingly, the present inventors completed the present invention by confirming that a synergistic effect of remarkably increasing anti-inflammatory efficacy is exhibited when a mixture of fermented gentian extract, chrysanthemum extract, and mint extract is used.

Korean Registered Patent No. 10-1910156

An object of the present invention is to provide a pharmaceutical, health functional food, health food and cosmetic composition for anti-inflammatory.

In order to achieve the above object,

The present invention is a fermented gentian extract; And Chrysanthemum ( Chrysanthemum indicum ) extract and peppermint ( Mentha piperascens ) extract; at least one selected from the group consisting of; containing, the fermentation gentian extract is gentian ( Gentiana It provides an anti-inflammatory pharmaceutical composition, health functional food composition, health food composition and cosmetic composition, characterized in that the extract is a fermented scabra ) yeast.

The anti-inflammatory composition containing the fermented gentian extract, chrysanthemum extract and mint extract according to the present invention contains a mixed extract of fermented gentian extract and at least one extract selected from the group consisting of chrysanthemum extract and mint extract, anti-inflammatory It has the effect of significantly improving the efficacy, and has no side effect because it does not affect the cell viability, so it can be usefully used as a natural anti-inflammatory pharmaceutical, health functional food, health food and cosmetic composition.

Hereinafter, the present invention will be described in detail.

Pharmaceutical composition for anti-inflammatory

The present invention is a fermented gentian extract; And

Chrysanthemum indicum extract and peppermint ( Mentha) piperascens ) contains at least one extract selected from the group consisting of extracts;

The fermentation gentian extract is gentian ( Gentiana scabra ) is a pharmaceutical composition for anti-inflammatory, characterized in that the extract is fermented with yeast.

In the pharmaceutical composition according to the present invention, the yeast may be Saccharomyces cerevisiae . In an embodiment of the present invention, the gentian fermentation extract was prepared by fermenting the gentian using dry yeast ( Saccaromyces cerevisiae KCCM 11201).

In the pharmaceutical composition according to the present invention, the fermentation gentian may be to use the root of the gentian ( Gentiana scabra ), the chrysanthemum may be to use the flowers of chrysanthemum ( Chrysanthemum indicum ), the peppermint peppermint ( Mentha piperascens ), but is not limited thereto.

The term "extract" used in the present invention refers to a preparation obtained by extracting a raw material to be extracted as it is or pulverizing it using an appropriate solvent, and evaporating the leachate, but is not limited thereto. It may be an extract obtained by, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a crude product or a purified product thereof. The extract may be prepared using general extraction methods, separation and purification methods known in the art. The extraction method is not limited to this, but is preferably solvent extraction, water vapor distillation, compression, oil absorption, volatile organic solvent extraction, or supercritical fluid extraction, hot water extraction, hot water extraction, cold extraction, reflux cooling extraction or ultrasonic Methods such as extraction may be used, and preferably, a method of solvent extraction or steam distillation may be used. At this time, any suitable solvent that can be used to obtain the extract may be any solvent acceptable in the art, and water or an organic solvent may be used. For example, alcohols having 1 to 4 carbon atoms, including purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, and ether , Benzene (benzene), chloroform (chloroform), ethyl acetate (ethyl acetate), methylene chloride (methylene chloride), hexane (hexane) and cyclohexane (cyclohexane) can be used alone or in combination, various solvents It is not limited.

Preferably, the chrysanthemum extract and peppermint extract are each extracted using one or more extraction solvents selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, hydrocarbon-based solvents, or mixed solvents thereof. It may be, and more preferably, it may be extracted using one or more extraction solvents selected from water or anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms.

In the pharmaceutical composition according to the present invention, the fermentation gentian extract, and one or more extracts selected from the group consisting of chrysanthemum extract and mint extract may be included in a volume ratio of 1.0: 0.1 to 3.0, preferably 1.0: 0.8 It may be included in a volume ratio of ~ 2.5. More preferably, the fermentation gentian extract and the chrysanthemum extract or the fermentation gentian extract and the mint extract are each contained in a volume ratio of 1: 0.8 to 1.2, or the fermentation gentian extract, the chrysanthemum extract and the mint extract are in a volume ratio of 1: 0.8 to 1.2: 0.8 to 1.2, respectively. It may be to include, fermentation gentian extract and chrysanthemum extract or fermentation gentian extract and peppermint extract in a volume ratio of 1: 0.9 to 1.1 respectively, or fermentation gentian extract, chrysanthemum extract and mint extract 1: 0.9 to 1.1: 0.9 ~ It is particularly preferred to include it in a volume ratio of 1.1.

The pharmaceutical composition according to the present invention may be administered in various dosage forms, oral and parenteral, during clinical administration, preferably oral administration, transdermal administration, topical administration, inhalation administration, intranasal administration, intravenous administration, intradermal administration It may be administered in any one or more formulations selected from the group consisting of administration, intraperitoneal administration, injection and subcutaneous injection formulations. When formulated, it is usually prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants.

Solid preparations for oral administration include tablets, patients, powders, granules, capsules, troches, etc. These solid preparations include at least one excipient such as starch, calcium carbonate in one or more of the present invention. It is prepared by mixing sucrose, lactose, or gelatin. In addition, lubricants such as magnesium stearate talc are used in addition to simple excipients. Liquid preparations for oral administration include suspending agents, intravenous solutions, emulsions or syrups, etc. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, flavoring agents, preservatives, etc. are included. Can be.

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspension solutions, emulsions, lyophilized preparations, suppositories, creams, gels, sprays, drops, and the like. As non-aqueous solvents and suspensions, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin, glycerol, gelatin, etc. may be used.

The effective dosage for the human body of the pharmaceutical composition of the present invention may vary depending on the patient's age, weight, sex, dosage form, health status and disease level, and is generally about 0.01-1000 mg / kg / day, preferably It may be 0.1-500 mg / kg / day, and may be dividedly administered once to several times a day at regular time intervals according to the judgment of a doctor or pharmacist.

The pharmaceutical composition of the present invention may further include a known anti-inflammatory agent in addition to fermentation gentian extract, chrysanthemum extract and mint extract as an active ingredient.

Anti-inflammatory health functional food and health food composition

The present invention is a fermented gentian extract; And

Chrysanthemum indicum extract and peppermint ( Mentha) piperascens ) contains at least one extract selected from the group consisting of extracts;

The fermentation gentian extract is gentian ( Gentiana It relates to a health functional food composition for anti-inflammatory, characterized in that the extract is fermented with scabra ) yeast.

The term "health functional food" used in the present invention refers to food prepared and processed in the form of tablets, capsules, powders, granules, liquids and pills using ingredients or ingredients having useful functional properties for the human body. Here, the term 'functional' refers to obtaining a useful effect for health use, such as regulating nutrients or physiological effects on the structure and function of the human body. The health functional food of the present invention can be manufactured by a method conventionally used in the general technical field, and may be prepared by adding raw materials and components conventionally added in the general technical field. In addition, the formulation of the health functional food can also be prepared without limitation as long as the formulation is recognized as a health functional food. The health functional food composition of the present invention can be manufactured in various types of formulations, and has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material, unlike general drugs, and has excellent portability. The health functional food of the present invention can be taken as an adjuvant to enhance the effectiveness of the anti-inflammatory agent.

In addition, the present invention is a fermented gentian extract; And

Chrysanthemum indicum extract and peppermint ( Mentha) piperascens ) contains at least one extract selected from the group consisting of extracts;

The fermentation gentian extract is gentian ( Gentiana scabra ) is an anti-inflammatory health food composition characterized in that it is an extract fermented with yeast.

When the composition of the present invention is used as a health functional food composition or a health food composition, one or more extracts selected from the fermentation gentian extract and chrysanthemum extract and peppermint extract can be added as it is or used with other foods or food ingredients, usually It can be used as appropriate according to the method. The composition may include a food additive that is food-acceptable in addition to the active ingredient, and the mixing amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health, or therapeutic treatment).

The term "food supplement additive" used in the present invention means a component that can be supplementally added to food, and is added to prepare a health functional food or health food of each formulation, and can be appropriately selected and used by those skilled in the art. Examples of food supplements include flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and fillers, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners , pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonic acid used in carbonated beverages, and the like, but the types of food supplements of the present invention are not limited by the examples.

In addition, there is no restriction on the type of health functional food or health food to which the composition of the present invention can be used. In addition, the composition comprising at least one extract selected from the fermented gentian extract and chrysanthemum extract and peppermint extract of the present invention as an active ingredient is mixed with other appropriate ingredients and known additives that may be contained in the health functional food according to the choice of those skilled in the art Can be produced. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, dairy products including gums, ice cream, various soups, beverages, teas, drinks, alcoholic beverages, and There are vitamin complexes and the like, and can be prepared by adding the extract according to the present invention as a main ingredient, and adding it to juice, tea, jelly, and juice.

Anti-inflammatory Cosmetic  Composition

The present invention is a fermented gentian extract; And

Chrysanthemum indicum extract and peppermint ( Mentha) piperascens ) contains at least one extract selected from the group consisting of extracts;

The fermentation gentian extract is gentian ( Gentiana It relates to a cosmetic composition for anti-inflammatory, characterized in that the extract is fermented with scabra ) yeast.

The cosmetic composition according to the present invention includes lotion, nutrient lotion, nutrient cream, moisture cream, massage cream, essence, paste, mask pack, patch, gel, cream, lotion, powder, soap, cleanser, oil, foundation, makeup base, wax And it may be one or more formulations selected from the group consisting of spray. Other ingredients other than the above-mentioned ingredients may be appropriately selected and blended without difficulty by those skilled in the art according to other formulations or purpose of use.

The cosmetic composition may be, for example, a solution, gel, solid or dough anhydrous product, emulsion obtained by dispersing an oil phase in an aqueous phase, suspension, microemulsion, microcapsule, microgranule or ionic (liposome), nonionic vesicle dispersant It can be provided in the form of, cream, skin, lotion, oil, powder, ointment, spray or conceal stick. It can also be prepared in the form of a foam or aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition in addition to the at least one extract selected from the fermentation gentian extract and chrysanthemum extract and peppermint extract of the present invention, fat substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, Stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion blockers and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, It may contain adjuvants commonly used in the cosmetic field, such as hydrophilic or lipophilic actives, lipid vesicles, or any other ingredient commonly used in cosmetics. These supplements are introduced in amounts commonly used in the field of cosmetic science.

In the cosmetic composition of the present invention, one or more extracts selected from the fermentation gentian extract and the chrysanthemum extract and the peppermint extract of the present invention in a cosmetic composition that is usually contained is 0.1 to 50% by weight, preferably 1 to 10% by weight It can be added in an amount.

When using at least one extract selected from fermentation gentian extract and chrysanthemum extract and peppermint extract according to the present invention as an external preparation for skin, additionally a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softening agent, an antioxidant, and a suspending agent , Stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion blockers and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments It may contain adjuvants commonly used in the field of skin science, such as, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in external preparations for skin. In addition, the ingredients can be introduced in an amount commonly used in the field of dermatology.

Fermentation gentian extract, chrysanthemum extract, and peppermint extract according to the present invention use natural plants as a raw material, so even when used as a pharmaceutical, food or cosmetic composition, the side effects may be less than that of a general synthetic compound, so that the pharmaceutical composition, health functional food safely , It can be usefully included in health food and cosmetic compositions.

Hereinafter, the present invention will be described in more detail by examples. It will be apparent to those skilled in the art that these examples are merely for more specifically describing the present invention, and the scope of the present invention is not limited to these examples.

< Manufacturing example  1> Fermentation Gentian  Preparation of extract

Dried gentian scabra Bunge) root (Chinese, Human Herb) 50g, purified water 500ml and dry yeast ( Saccaromyces cerevisiae , Choheung Co., Ltd.) 0.5g was mixed, followed by incubation at 30 ° C at 100 rpm for 24 hours. This was filtered through a filter paper (Whatman No. 1, USA), filtered again with a 0.2 μm filter (Sartorius, Germany), and sterilized to prepare an extract for fermentation.

< Manufacturing example  2> Preparation of chrysanthemum extract

50 g of dried chrysanthemum ( Chrysanthemum indicum ) flowers (Yeongcheon, Gyeongbuk, Human Herb) were mixed with 500 ml of purified water, heated to 80 ° C and extracted for 1 hour. This was filtered through a filter paper (Whatman No. 1, USA), filtered again with a 0.2 μm filter (Sartorius, Germany), and sterilized to prepare a chrysanthemum extract.

< Manufacturing example  3> Preparation of peppermint extract

Dried mint ( Mentha piperascens ) 50 g of leaves (Yeongcheon, Gyeongbuk, and Human Hub) were mixed with 500 ml of purified water, respectively, and heated to 80 ° C to extract for 1 hour. This was filtered through a filter paper (Whatman No. 1, USA), filtered again with a 0.2 μm filter (Sartorius, Germany), and sterilized to prepare a mint extract.

< Experimental Example  1> Cytotoxicity measurement

Cytotoxicity was evaluated for a mixed extract obtained by mixing the fermented gentian extract, chrysanthemum extract, and peppermint extract alone and two or three extracts in the same parts by weight, prepared by the methods of Preparation Examples 1 to 3 above.

Specifically, 5 × 10 ⁴ per well in a 96-well plate using DMEM medium (Gibco, USA) containing 10% fetal bovine serum (Gibco, USA) of mouse macrophage cell line RAW264.7 cells (Korea Research Institute of Bioscience and Biotechnology). After dispensing with the number of cells and cultured for 24 hours, the sample was exchanged with serum-free DMEM medium to which a single extract or a mixed extract sample was added. As shown in Table 1 below, the treatment of each sample was treated with 0.5% (v / v) of the total volume of the medium, and 0.5% (v / v) of each extract even when two or three extracts were combined. v) were mixed and treated. After incubation for 24 hours, 20 μl of each solution was dissolved in 5 mg / ml of MTT (thiazolyl blue tetrazolium bromide, Sigma Aldrich, USA) in phosphate-buffered saline (PBS), and then cultured for 3 hours. The medium was removed and DMSO (dimethyl sulfoxide, Amresco, USA) was added at 100 μl to dissolve the generated formazan crystal, and absorbance at 570 nm was measured using a microplate reader (Infinite F200, Tecan, Switzerland).

Unit:% (v / v) Fermentation gentian extract
(Production Example 1) Chrysanthemum extract
(Production Example 2) Peppermint extract
(Production Example 3) Example 1 0.5 0.5 0.5 Example 2 0.5 0.5 - Example 3 0.5 - 0.5 Comparative Example 1 0.5 - - Comparative Example 2 - 0.5 - Comparative Example 3 - - 0.5

Cell viability according to each sample treatment was confirmed using the following Equation 1, and the results are shown in Table 2 below.

[Equation 1]

Cell viability (%) = A / B × 100

(A is the absorbance of each sample, and B is the absorbance of the control group (no sample added).)

sample Cell viability (%) Example 1 Fermentation gentian + chrysanthemum + mint 102.78 ± 2.42 Example 2 Fermentation gentian + chrysanthemum 109.59 ± 2.27 Example 3 Fermentation Gentian + Mint 104.90 ± 1.99 Comparative Example 1 Fermentation Gentian 111.89 ± 3.28 Comparative Example 2 Chrysanthemum 116.71 ± 1.64 Comparative Example 3 mint 103.06 ± 0.75

As shown in Table 3, the survival rate of RAW264.7 cells was 100% or more when each extract was treated with 0.5% alone or mixed, and the fermentation gentian extract, chrysanthemum extract, mint extract and their It was confirmed that the mixed extracts of 2 or 3 were not toxic.

< Experimental Example  2> Nitric oxide (NO) production measurement (anti-inflammatory activity evaluation)

After dispensing the mouse macrophage cell line RAW264.7 cells (Korea Research Institute of Bioscience and Biotechnology) with DMEM medium containing 10% fetal bovine serum, dispensing the cells into 96-well plates with 5 × 10 ⁴ cells per well and incubating for 24 hours. The samples were exchanged with serum-free DMEM medium to which extract or mixed extract samples were added. As shown in Table 1, as in Experimental Example 1, the treatment of each sample was treated with 0.5% (v / v) of the total volume of the medium, and each of the two or three extracts was combined. The extracts were treated by mixing in 0.5% (v / v) increments. After 1 hour, lipopolysaccharide (Sigma Aldrich, USA) was added to 1 μg / ml and cultured for 24 hours. 60 μl of the medium and 60 μl of Griess reagent (Sigma Aldrich, USA) were mixed, and after 10 minutes, the absorbance was measured at 570 nm using a microplate reader (Infinite F200, Tecan, Switzerland). The inhibition rate of NO production according to each sample treatment was confirmed using Equation 2 below, and the results are shown in Table 3 below.

[Equation 2]

NO production inhibition rate (%) = [1- (A / B)] × 100

(A is the absorbance of each sample, and B is the absorbance of the control group (no sample added).)

sample NO production inhibition rate (%) Example 1 Fermentation gentian + chrysanthemum + mint 74.86 ± 3.02 Example 2 Fermentation gentian + chrysanthemum 69.88 ± 3.75 Example 3 Fermentation Gentian + Mint 48.31 ± 3.21 Comparative Example 1 Fermentation Gentian 29.16 ± 0.71 Comparative Example 2 Chrysanthemum 45.56 ± 1.14 Comparative Example 3 mint 11.78 ± 1.11

As shown in Table 4, the mixed extracts of Examples 1 to 3 in which two or three types of fermented gentian extract, chrysanthemum extract and peppermint extract were mixed show higher NO production inhibition rate than the single extracts of Comparative Examples 1 to 3. In particular, the fermentation gentian, chrysanthemum and peppermint extract of Example 1 exhibited the highest inhibition rate of NO production of 74.86%.

Therefore, it was confirmed that when the fermentation gentian, chrysanthemum and mint extract were mixed, a synergistic effect on anti-inflammatory activity appeared, from which the mixed extract can be usefully used as a new anti-inflammatory composition and skin soothing efficacy cosmetic composition. Confirmed.

So far, the present invention has been focused on the preferred embodiments. Those skilled in the art to which the present invention pertains will appreciate that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered in terms of explanation, not limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent range should be interpreted as being included in the present invention.

Claims (9)

Fermented gentian extract; And
Chrysanthemum indicum extract and peppermint ( Mentha) piperascens ) contains at least one extract selected from the group consisting of extracts;
The fermentation gentian extract is gentian ( Gentiana scabra ) is a pharmaceutical composition for anti-inflammatory, characterized in that the extract is fermented with yeast.
According to claim 1,
The yeast is Saccharomyces Saccharomyces cerevisiae ).
According to claim 1,
The chrysanthemum extract and peppermint extract are each extracted using one or more extraction solvents selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, hydrocarbon-based solvents, or mixed solvents thereof. Pharmaceutical composition.
According to claim 1,
A pharmaceutical composition comprising one or more extracts selected from the group consisting of the fermentation gentian extract and chrysanthemum extract and mint extract in a volume ratio of 1.0: 0.1 to 3.0.
The method of claim 4,
A pharmaceutical composition comprising at least one extract selected from the group consisting of the fermentation gentian extract and chrysanthemum extract and peppermint mint extract in a volume ratio of 1.0: 0.8-2.5.
Fermented gentian extract; And
Chrysanthemum indicum extract and peppermint ( Mentha piperascens ) contains at least one extract selected from the group consisting of extracts;
The fermentation gentian extract is gentian ( Gentiana scabra ) is a health functional food composition for anti-inflammatory, characterized in that the extract is fermented with yeast.
Fermented gentian extract; And
Chrysanthemum indicum extract and peppermint ( Mentha) piperascens ) contains at least one extract selected from the group consisting of extracts;
The fermentation gentian extract is gentian ( Gentiana scabra ) is a health food composition for anti-inflammatory, characterized in that the extract is fermented with yeast.
Fermented gentian extract; And
Chrysanthemum indicum extract and peppermint ( Mentha) piperascens ) contains at least one extract selected from the group consisting of extracts;
The fermentation gentian extract is gentian ( Gentiana scabra ) cosmetic composition for anti-inflammatory, characterized in that the extract is fermented with yeast.
The method of claim 8,
The cosmetic composition is a lotion, nutrition lotion, nutrition cream, moisture cream, massage cream, essence, paste, mask pack, patch, gel, cream, lotion, powder, soap, cleanser, oil, foundation, makeup base, wax and spray Cosmetic composition characterized in that it is one or more formulations selected from the group consisting of.