JP6146265B2 - 顕微鏡システムおよびオートフォーカス方法 - Google Patents

顕微鏡システムおよびオートフォーカス方法 Download PDF

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Publication number
JP6146265B2
JP6146265B2 JP2013230998A JP2013230998A JP6146265B2 JP 6146265 B2 JP6146265 B2 JP 6146265B2 JP 2013230998 A JP2013230998 A JP 2013230998A JP 2013230998 A JP2013230998 A JP 2013230998A JP 6146265 B2 JP6146265 B2 JP 6146265B2
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Japan
Prior art keywords
stage
movement
bright field
image
microscope system
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JP2013230998A
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English (en)
Japanese (ja)
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JP2015090471A (ja
JP2015090471A5 (enExample
Inventor
真司 渡辺
真司 渡辺
一樹 相坂
一樹 相坂
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Sony Corp
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Sony Corp
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Priority to JP2013230998A priority Critical patent/JP6146265B2/ja
Priority to US14/903,120 priority patent/US10502943B2/en
Priority to PCT/JP2014/005459 priority patent/WO2015068360A1/en
Priority to EP14796559.4A priority patent/EP3066513B1/en
Publication of JP2015090471A publication Critical patent/JP2015090471A/ja
Publication of JP2015090471A5 publication Critical patent/JP2015090471A5/ja
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/24—Base structure
    • G02B21/241—Devices for focusing
    • G02B21/244—Devices for focusing using image analysis techniques
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G02B21/0084—Details of detection or image processing, including general computer control time-scale detection, e.g. strobed, ultra-fast, heterodyne detection
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/12—Condensers affording bright-field illumination
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Multimedia (AREA)
  • General Engineering & Computer Science (AREA)
  • Microscoopes, Condenser (AREA)
  • Automatic Focus Adjustment (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Focusing (AREA)
  • Studio Devices (AREA)
JP2013230998A 2013-11-07 2013-11-07 顕微鏡システムおよびオートフォーカス方法 Active JP6146265B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2013230998A JP6146265B2 (ja) 2013-11-07 2013-11-07 顕微鏡システムおよびオートフォーカス方法
US14/903,120 US10502943B2 (en) 2013-11-07 2014-10-29 Microscope system and autofocusing method
PCT/JP2014/005459 WO2015068360A1 (en) 2013-11-07 2014-10-29 Microscope system and autofocusing method
EP14796559.4A EP3066513B1 (en) 2013-11-07 2014-10-29 Microscope system and autofocusing method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2013230998A JP6146265B2 (ja) 2013-11-07 2013-11-07 顕微鏡システムおよびオートフォーカス方法

Publications (3)

Publication Number Publication Date
JP2015090471A JP2015090471A (ja) 2015-05-11
JP2015090471A5 JP2015090471A5 (enExample) 2016-04-14
JP6146265B2 true JP6146265B2 (ja) 2017-06-14

Family

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Family Applications (1)

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JP2013230998A Active JP6146265B2 (ja) 2013-11-07 2013-11-07 顕微鏡システムおよびオートフォーカス方法

Country Status (4)

Country Link
US (1) US10502943B2 (enExample)
EP (1) EP3066513B1 (enExample)
JP (1) JP6146265B2 (enExample)
WO (1) WO2015068360A1 (enExample)

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US10469851B2 (en) * 2012-04-16 2019-11-05 New Cinema, LLC Advanced video coding method, system, apparatus, and storage medium
JP5874753B2 (ja) * 2014-01-28 2016-03-02 カシオ計算機株式会社 撮像装置、撮像方法及びプログラム
DE102015219709A1 (de) * 2015-10-12 2017-04-13 Carl Zeiss Microscopy Gmbh Bildkorrekturverfahren und Mikroskop
DE112016005953T5 (de) * 2015-12-25 2018-10-04 Keyence Corporation Konfokaler verschiebungssensor
US10440025B2 (en) 2016-06-07 2019-10-08 Gryphon Online Safety, Inc Remotely controlling access to online content
US11301572B2 (en) 2016-02-27 2022-04-12 Gryphon Online Safety, Inc. Remotely controlling access to online content
US12348520B2 (en) 2016-02-27 2025-07-01 Gryphon Online Safety, Inc Remotely controlling access to online content
DE102017107348B4 (de) 2017-04-05 2019-03-14 Olympus Soft Imaging Solutions Gmbh Verfahren zur zytometrischen Analyse von Zellproben
CN110692235B (zh) * 2017-05-17 2021-06-22 株式会社克利普顿 图像处理装置、图像处理程序及图像处理方法
WO2019172097A1 (ja) * 2018-03-07 2019-09-12 コニカミノルタ株式会社 画像処理方法、画像処理装置及びプログラム
JP2022001889A (ja) * 2018-09-27 2022-01-06 富士フイルム株式会社 フォーカス位置評価装置、方法及びプログラム、並びに判別器
WO2020071499A1 (ja) * 2018-10-05 2020-04-09 富士フイルム株式会社 フォーカス位置評価装置、方法、及びプログラム
WO2020244775A1 (en) * 2019-06-07 2020-12-10 Leica Microsystems Cms Gmbh A system and method for processing biology-related data, a system and method for controlling a microscope and a microscope
CN120334192A (zh) * 2019-07-09 2025-07-18 索尼集团公司 信息处理装置和程序
FR3117717B1 (fr) * 2020-12-10 2022-12-23 Centre Nat Etd Spatiales Procédé d’acquisition et d’analyse d’une scène par différence d’images

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DE2529735C3 (de) * 1975-07-01 1978-06-08 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V., 3400 Goettingen Korpuskularstrahlmikroskop, insbe- · sondere Elektronenmikroskop, mit Verstelleinrichtungen zur Änderung der Lage des abzubildenden Objekts und Verfahren zum Betrieb
CN101151378B (zh) * 2005-03-30 2013-04-03 奥林巴斯株式会社 规定部位发光量测定方法、规定部位发光量测定装置、表达量测定方法及测定装置
ATE529894T1 (de) * 2006-01-25 2011-11-15 Nxp Bv Nanodraht-tunneltransistor
CN101438147B (zh) * 2006-05-31 2011-09-28 奥林巴斯株式会社 生物试样摄像方法及生物试样摄像装置
EP2034349B2 (en) * 2006-06-08 2023-07-19 Nikon Corporation Observing apparatus and observing method
JP4973162B2 (ja) * 2006-12-01 2012-07-11 株式会社ニコン 画像処理装置、画像処理プログラム及び観察システム
JP4937850B2 (ja) * 2007-07-03 2012-05-23 オリンパス株式会社 顕微鏡システム、そのｖｓ画像生成方法、プログラム
WO2009025345A1 (ja) * 2007-08-22 2009-02-26 Nikon Corporation 撮影制御装置、顕微鏡、および、プログラム
JP5045320B2 (ja) 2007-09-05 2012-10-10 ソニー株式会社 画像処理装置、および画像処理方法、並びにコンピュータ・プログラム
JP5185704B2 (ja) 2007-12-26 2013-04-17 オリンパス株式会社 生体観察装置
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JP2012065257A (ja) * 2010-09-17 2012-03-29 Olympus Corp 顕微鏡用撮像装置
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JP5887766B2 (ja) * 2011-08-31 2016-03-16 株式会社ニコン 顕微鏡制御装置、画像処理装置、顕微鏡装置およびプログラム
JP5803442B2 (ja) * 2011-08-31 2015-11-04 株式会社ニコン 顕微鏡制御装置、顕微鏡装置、画像処理装置およびプログラム
US20140087463A1 (en) * 2012-09-25 2014-03-27 The Washington University Optical control of cell signaling

Also Published As

Publication number Publication date
WO2015068360A1 (en) 2015-05-14
JP2015090471A (ja) 2015-05-11
US10502943B2 (en) 2019-12-10
US20160246045A1 (en) 2016-08-25
EP3066513B1 (en) 2021-03-03
EP3066513A1 (en) 2016-09-14

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