JP6139048B2 - Cosmetics for improving pore conspicuousness - Google Patents

Description

  The present invention relates to a cosmetic for improving pore conspicuousness and an agent for improving pore conspicuousness, comprising a humus soil extract as an active ingredient.

The conspicuity of pores occupies the top as a skin problem to be improved during skin care. Causes of the conspicuous pores include square plugs formed in the pores, pigmentation, the extent of pore openings and the dents in the periphery. Among these, for horn plugs, various horn plug removers have been developed and widely used.
In order to make pores inconspicuous, natural polysaccharides such as Hibamata (see Patent Document 1), phosphorylated glyceryl ether derivatives having an alkyl group having 8 to 32 carbon atoms (see Patent Document 2), active vitamin D3 or Various pore conspicuity improving agents such as a pore conspicuousness improving agent containing the derivative as an active ingredient and a pore contractor containing active vitamin D3 or a derivative thereof (see Patent Document 3) have been proposed. As the needs of consumers diversify, there is a need for an agent for improving pore conspicuousness according to the needs.

By the way, humus soil is a ground plant, a large-scale microalgae plant (broad sense), seafood, and other minerals deposited on the bottom of the sea, swamps, ponds and lakes, or those that have risen on the surface, or forests. Plants (in a broad sense) such as those deposited on the surface of the earth and sediments containing them have been decomposed and organically formed by anaerobic microorganisms over a long period of time.
The technique regarding the water extract manufacturing method of this humus soil is known (refer patent document 4 and patent document 5). Moreover, the humus soil extract is known to have a sterilizing effect and a hair damage reducing effect (see Patent Documents 6 and 7). However, the effectiveness of humus soil extract is still unclear.

JP 2000-169322 A JP 2002-187817 A JP 2004-339120 A JP 2000-136140 A JP 2006-181460 A JP 2003-267821 A JP 2010-270063 A

  The present invention provides a cosmetic for improving pore conspicuousness and an agent for improving pore conspicuousness, which have excellent effects.

  As a result of various studies to solve the above problems, the present invention has newly found that the humus soil extract has an effect of improving pore conspicuousness. Providing agents and cosmetics.

That is, the present invention relates to the following [1] and [ 2 ].
[1] Solid content concentration When converted to an aqueous solution of 0.5% by mass, a humus soil extract containing 100 mg / L or more of humic substance is used as an active ingredient, and the blending amount of the humus soil extract is expressed as a solid content concentration. A cosmetic for improving pore conspicuousness of 0.02 to 0.04% by mass .
[2 ] It is preferable that the humus soil extract is obtained from at least one selected from water and alcohols.

  ADVANTAGE OF THE INVENTION According to this invention, the pore conspicuous improvement cosmetics and pore conspicuous improvement agent which improve the outstanding pore conspicuousness can be provided.

  Hereinafter, the present invention will be described in detail.

The humus soil of the humus soil extract used in the present invention can be used regardless of the production area or state. Specifically, as this humus soil, for example, those originating from forests, rivers, lakes, oceans, etc. can be used. Plants such as microalgae (in a broad sense) and sediments containing them have been decomposed, synthesized and organically produced by anaerobic microorganisms. Of these, humus derived from marine sediments, more preferably humus derived from the Japanese archipelago, where marine sediments are raised, and humus from Kyushu is preferred. .
Although the degree of humus of this humus soil is not particularly limited, it is preferable that humus progresses and contains fulvic acid or humic acid, which are high molecular organic compounds.

The humus soil extract used in the present invention is obtained by bringing the humus soil into contact with an extraction solvent by an extraction means, obtaining a component having an effect on improving pore conspicuousness, and collecting the component. Furthermore, it is preferable to perform filtration means for removing unnecessary substances and sterilization.
The humus soil extract thus obtained may be diluted, concentrated or dried as necessary, and may be separated or purified to remove impurities.
The form of the humus soil extract is not particularly limited, and examples thereof include solid, semi-solid, and liquid. Specific examples include states such as solutions, suspensions, concentrates, emulsions, slurries, powders, granules, and solids.

  An example of a method for producing a suitable humus soil extract is to obtain a humus soil extract by adding an extraction solvent to the humus soil, mixing and stirring, and then separating active ingredients.

Examples of the extraction means include solid-liquid extraction, liquid-liquid extraction, immersion, decoction, leaching, reflux extraction, supercritical fluid extraction, ultrasonic extraction, microwave extraction, mixing and stirring, and the like. You may combine these suitably.
The separation means is not particularly limited, and examples thereof include filtration separation means and centrifugal separation means. These may be used alone or in combination.
Examples of the filtration separation means include natural filtration, vacuum filtration, and pressure filtration. At this time, a filter medium such as a cellulose filter, a glass fiber filter and a membrane filter is used, and a filter aid such as celite, gravel and activated carbon is used as necessary. The pore diameter is not particularly limited, but for example 0.1 to 1 μm is suitable. You may combine these suitably.
In addition, there is no particular limitation on the method and method of drying and pretreatment of humus soil prior to extraction, and the extraction means such as the ratio of humus soil and solvent, extraction time, etc. are not particularly limited.

  The extraction solvent is not particularly limited, and either a polar solvent or a nonpolar solvent may be used. Examples of the extraction solvent include water (water containing minerals such as hot spring water and deep sea water and purified water); linear, branched or cyclic alcohols; ketones such as acetone and methyl ethyl ketone; methyl acetate Esters such as ethyl acetate; Chain and cyclic ethers such as tetrahydrofuran and diethyl ether; Polyethers such as polyethylene glycol; Hydrocarbons such as squalane, hexane, cyclohexane and petroleum ether; Aromatic hydrocarbons such as toluene And halogenated hydrocarbons such as dichloromethane, chloroform and dichloroethane; and supercritical carbon dioxide. In addition, you may use these as a mixed solvent which was individual or combined 2 or more types. The organic solvent is preferably water-soluble.

  Among the extraction solvents, one or more selected from water, alcohols, ketones and supercritical carbon dioxide are preferable. Among them, one or more selected from water and alcohols are preferable, and examples thereof include water, alcohols, and a mixture of water and alcohols.

Here, the alcohols may be either monohydric or polyhydric alcohols. Examples of monohydric alcohols include methanol, ethanol and propanol. Examples of polyhydric alcohols include ethylene glycol. , Propylene glycol, 1,3-butylene glycol, 1,2-pentanediol, glycerin and dipropylene glycol.
Among the alcohols, for example, lower alcohols having 1 to 5 carbon atoms such as ethanol and 1,3-butylene glycol are preferable, among which lower monohydric alcohols are preferable, and among these, ethanol is more preferable.

  Among these, water and a hydroalcoholic mixture are further preferred. Specifically, an aqueous solution containing 0 to 90% by volume of alcohol is preferable, preferably an aqueous solution containing 0 to 70% by volume, more preferably 0 to 50% by volume, and still more preferably 0 to 20% by volume. It is.

  Although the usage-amount of the said solvent is not specifically limited, For example, it is preferable to set it as 100-1000L with respect to 100 kg of humus soil (dry state), it is more preferable to set it as 200-600L, and the extraction efficiency of humus soil component And it is preferable in terms of work efficiency.

The extraction period is not particularly limited, but is preferably 1 day to 3 years, more preferably 10 to 180 days.
The mixing and stirring of the humus soil and the extraction solvent at this time is not particularly limited, and may be performed for about 0.5 to 48 hours, for example. Thereby, humus soil and an extraction solvent contact and it becomes easy to extract an active ingredient. Moreover, what is necessary is just to perform mixing stirring continuously or discontinuously.
For example, after mixing and stirring, it is preferable from the standpoint of extraction efficiency to further mix and stir for a certain period, or to leave after mixing and stirring for a certain period, or to stand and mature. It is preferable that the humus soil settles by leaving it after mixing and stirring, which is advantageous for separation.
For example, the mixture is stirred continuously or discontinuously within the extraction period (for example, 1 day to 3 years); after 1 to 24 hours of mixing and stirring, the mixture is left to stand for 1 to 60 days (preferably 20 to 40 days); 1 For example, after 24 hours of mixing and stirring, the mixture is stirred for 1 to 20 days (preferably 3 to 9 days) and then left for 1 to 3 years (preferably 6 to 2 years).

  The extraction temperature is not particularly limited, and is preferably low temperature to high temperature (for example, about 0 to 100 ° C.), more preferably low temperature (for example, about 0 to 9 ° C.) to normal temperature (for example, about 10 to 40 ° C.). It is preferable to prevent the humus soil from being heat denatured.

  Examples of the drying include sun drying, natural drying, wind drying, heat drying, freeze drying, and the like, and it is preferable if the humus soil is not thermally denatured. At this time, it is desirable to dry the humus soil so that it can be finely pulverized, and the humus soil extraction efficiency is improved by pulverization, which is preferable.

  The humus soil extract contains humic soil-specific constituents (humic substances and the like) such as fulvic acid and humic acid, fatty acids, organic acids, amino acids, proteins, minerals, and the like. It is preferable that the humus soil extract is filtered using a sterilizing filter because the humus soil is not thermally denatured and insoluble substances are removed.

The humus soil extract has a humic substance of at least 100 mg / L or more, preferably 100 to 1000 mg / L, more preferably 200 to 700 mg / L, when converted to an aqueous solution with a solid content of 0.5% by mass. It is preferable that 350-650 mg / L is contained.
Here, the humic substance (humic substance) refers to, for example, a high-molecular substance generated by decomposition and condensation of plant components and the like in the soil. Here, the humic substance is a value when measured by the method described in the humic substance measurement method of the mineral spring test method.

In the humus soil extract, humic acid is preferably 0.1 to 15 mg / L, more preferably 1 to 10 mg / L, and further preferably 2 to 2 when converted to an aqueous solution with a solid content concentration of 0.5% by mass. It is suitable to contain 8 mg / L, more preferably 5-8 mg / L.
In the humus soil extract, fulvic acid is preferably 1 to 20 mg / L, more preferably 5 to 15 mg / L, and still more preferably 5 to 13 mg / L when converted to an aqueous solution with a solid content of 0.5% by mass. L, more preferably 8 to 13 mg / L is contained.
The mixing ratio of humic acid: fulvic acid at this time is not particularly limited, but is preferably 1:10 to 10: 1.

Here, humic acid refers to the humic substance that is alkali-soluble and precipitates in the acidic region. Although the detailed chemical structure is unknown, it is a condensate of a polyhydric phenol type aromatic compound and a nitrogen-containing compound, and has a phenolic hydroxyl group and a carboxyl group.
In addition, fulvic acid refers to an amorphous high-molecular organic acid that does not precipitate by acid among humic substances, which are end products that are decomposed by microorganisms such as plants. Chelating action that adsorbs and releases heavy metals, etc. It has something.
Further, quantification methods for humic acid and fulvic acid are described in “Soil Science Plant Nutrition, 38, 23-30 (Kuwatsuka A et al. 1992); Soil Science Plant Nutrition, 40, 601-608 (Watanabe A. et al. 1994); Humic Substances Reseacrh, Vol. 1, pp. 18-28 (Watanabe A. et al. 2004) ”.

The pH of the humus soil extract is preferably in the acidic region, preferably pH 1 to 6, more preferably pH 2 to 5 in terms of pharmacological activity and stability. At this time, a solid concentration of 0.1 to 1% by mass may be used, and the pH may be adjusted appropriately at 20 ° C.
The pH adjuster is not particularly limited as long as it is usually used. For example, inorganic acids such as hydrochloric acid, sulfuric acid, nitric acid, carbonic acid, phosphoric acid; citric acid, acetic acid, lactic acid, succinic acid, glycolic acid, Organic acids such as ascorbic acid, malic acid, fumaric acid, tartaric acid, urea, ε-aminocaproic acid and pyrrolidone carboxylic acid; betaines such as glycine betaine and lysine betaine; metals such as sodium hydroxide, potassium hydroxide and calcium hydroxide Inorganic alkalis such as hydroxides (alkali metal hydroxides and alkaline earth metal hydroxides); organic amines such as guanidine and 2-amino-2-methylpropane; ammonia, monoethanolamine, diethanolamine, tri Ethanolamine, isopropanolamine, diisopropanolamine, etc. Luke alkanolamine; arginine, basic amino acids such as lysine, and combinations thereof.

As shown in the below-mentioned Examples, the humus soil extract can exhibit the effects of improving pore conspicuousness, shrinking pores and improving the smoothness of the skin surface. Therefore, the humus soil extract can be applied, administered and ingested to animals including humans, and used for improving pore conspicuousness, shrinking pores and improving the smoothness of the skin surface. Further, the humus soil extract can be a pore conspicuousness improving agent and can be used for producing the same.
Therefore, the humus soil extract is useful as a material or formulation for blending in cosmetics, external preparations for skin, quasi drugs, pharmaceuticals, etc. for improving pore conspicuousness and smoothness of the skin surface. .

The usage form of the pore conspicuousness improving agent containing the humus soil extract is not particularly limited. Among these, cosmetics, external preparations for skin and quasi drugs are preferred, and the humus soil extract is suitable for use in cosmetics and external preparations for skin because its effect on the skin is recognized.
Examples of the use forms of the cosmetics and the external preparation for skin include, for example, cosmetics such as emulsions, creams, lotions, cosmetics, packs, cleaning agents, makeup cosmetics, hair growth agents, shampoos, and conditioners; It may be an external preparation for skin (external medicine) such as ointment, liquid, aerosol, patch, poultice, liniment.

  In addition, in the preparation, as necessary, various components usually used in the above-mentioned cosmetics and skin external preparations (eg, water, alcohol, oil agent, surfactant, thickener, Powders, chelating agents, pH adjusters, moisturizers, whitening agents, anti-inflammatory agents, cell activators, and other medicinal agents, extracts from animals, plants, microorganisms, and fragrances) that do not impair the effects of the present invention And may be blended as appropriate.

The blending amount of the humus soil extract is preferably 0.0001 to 1% by mass, preferably 0.0001 to 0.1% by mass as a solid content concentration in the preparation, from the viewpoint of utility. Use in such amounts is preferred.
Further, the pH (25 ° C.) of the preparation is preferably in the acidic to neutral range, more preferably 2 to 7, and further preferably 3 to 7 in view of utility. It is preferable to use it on the skin at such a pH.

Hereinafter, examples and examples will be given to specifically describe the present invention, but the present invention is not limited thereto.

<Manufacture example 1 of humus soil extract>
The corrosive soil collected from the ground (soil near the coast of the Kyushu region) was dried and then pulverized. 5 kg of this pulverized product and 20 liters of purified water were mixed and stirred for 2 hours, further stirred at room temperature (about 10 to 30 ° C.) for 7 days, and allowed to stand for 20 days. After leaving still, it filtered using a membrane filter (pore diameter 0.45 micrometer), and obtained humus soil extract (stock solution). The pH (20 ° C.) at this time was 3.0. Moreover, this dry solid content (solid content concentration) was 0.4 mass%.
The total amount of humic substances contained in this undiluted solution is 520 mg / L (650 mg / L when converted to an aqueous solution with a dry solid content of 5 g / water of 1 L), and the contents of humic acid and fulvic acid are 6 mg / L and 10 mg / L (7.5 mg / L and 12.5 mg / L, respectively, when converted to an aqueous solution having a dry solid content of 5 g / water of 1 L). This aqueous solution was used as the humus soil extract of Production Example 1.

<Manufacture example 2 of humus soil extract>
The corrosive soil collected from the ground (paddy field in Nagasaki Prefecture) was dried and then pulverized. 5 kg of this pulverized product and 20 liters of an ethanol aqueous solution containing 20% (v / v) ethanol were mixed and stirred for 2 hours, and further left to stand in a cool dark place (about 4 ° C.) for 20 days for aging. After standing, the mixture was filtered using a membrane filter (pore size: 0.3 μm) to obtain a corrosive earth extract (stock solution). The pH (20 ° C.) at this time was 2.6. Moreover, this dry solid content (solid content concentration) was 0.5 mass%.
The total amount of humic substances contained in the undiluted solution is 350 mg / L (350 mg / L when converted to an aqueous solution having a dry solid content of 5 g / water of 1 L), and the contents of humic acid and fulvic acid are 5 mg / L and 8. 5 mg / L (5 mg / L and 8.5 mg / L, respectively, when converted to an aqueous solution having a dry solid content of 5 g / water of 1 L). This aqueous solution was used as the humus soil extract of Production Example 2.

  These humic acids and fulvic acids were quantified in accordance with the above-mentioned reference “Soil Science Plant Nutrition, Vol. 40, pages 601-608 (Watanabe A. et al. 1994)”.

Example 1 and Comparative Example 1: Evaluation of gelling agent for reducing pore conspicuousness According to the following production method 1, a gel preparation of Example 1 was prepared, and a gel preparation of Comparative Example 1 containing no prescription 1 was prepared. In Formulation 1, the stock solution obtained in Production Example 1 was used. At this time, the pH of Example 1 and Comparative Example 1 was adjusted to the same using KOH.

(Production method 1: see Table 1)
A. Formulations 1 to 5 and a part of formulation 7 are mixed and dissolved uniformly.
B. Formula A is added to A and the pH is adjusted to 6.8.
C. Add the remaining amount of Formula 7 to B to adjust the gel.

(Evaluation of gel preparation)
For each of the seven panel members who are concerned about the conspicuous pores, the gel formulation of Example 1 is included in one of the left and right cheeks, and the gel formulation of Comparative Example 1 that does not contain a corrosive soil water extract, It was applied twice a day for 3 weeks. Take a face image before and after use, specify about 4x4cm on the left and right cheeks, calculate the total area and number of black parts when binarized by image analysis, and calculate the total pore area and total number of pores as panel The average value of all members was obtained. Table 2 summarizes the results after 3 weeks, with 100% before the test.

Examples 2 and 3, Reference Examples 4 and 5 and Comparative Example 2: Evaluation of skin pores for reducing pore conspicuousness According to the following production method 2, skin lotions of Examples 2 and 3 and Reference Examples 4 and 5 were prepared. A lotion of Comparative Example 2 containing no formulation 1 was prepared. In Formulation 1, the stock solution obtained in Production Example 1 was used.

(Production method 2: see Table 3)
A. Formulas 1-6 are mixed and dissolved.
B. Formulas 7-9 are mixed and dissolved.
C. A lotion was obtained by mixing B with A.

(Evaluation of lotion 1)
To each of the five panelists who are concerned about the pores, the skin lotion of Example 2 in which the humus soil extract prepared above was blended on one of the left and right cheeks, and the corrosive soil extract on the other. No lotion of Comparative Example 2 was applied twice a day for 2 weeks. Take a face image before and after use, specify about 4x4cm on the left and right cheeks, calculate the total area and number of black parts when binarized by image analysis, and calculate the total pore area and total number of pores as panel The average value of all members was obtained. Moreover, it carried out similarly about the lotion of Example 3 and Reference Examples 4 and 5. Table 3 summarizes the results after 3 weeks, with 100% before the test. In addition, the comparative example 2 showed the average value of the total.

(Evaluation of lotion 2)
With respect to the panel on which skin lotion evaluation 1 was performed, the conspicuous pores and the smoothness of the skin surface were visually determined in the following four stages, and the average value was determined.

(Evaluation criteria for conspicuous pores)
1: Pore is not conspicuous 2: Pore is conspicuous 3: Pore is conspicuous 4: Pore is very conspicuous

(Evaluation criteria for smoothness of skin surface)
1: The skin surface is very smooth 2: The skin surface is slightly smooth 3: The skin surface is slightly rough and not smooth 4: The skin surface is rough and not smooth

(Evaluation result 1)
From the results, it can be understood that by applying a preparation containing a humus soil extract, the effect of reducing pore area is higher, and that the effect of reducing the conspicuousness of the pores can be achieved by applying a preparation containing humus soil extract. .

  On the side of the comparative lotion application, the conspicuous pores and the smoothness of the skin surface were hardly changed, whereas the lotion application side containing the humus soil extract had an effect of reducing the conspicuous pores and the smoothness of the skin surface. It can be understood that the effect is improved.

[Example 3: Latex]
(Ingredient) (mass%)
1 Polyoxyethylene monostearate (20E.O.) sorbitan 1.0
2 Polyoxyethylene trioleate (20E.O.) sorbitan 0.5
3 Glyceryl monostearate 1.0
4 Stearic acid 0.5
5 Behenyl alcohol 0.5
6 Squalane 8.0
7 Carboxyvinyl polymer 0.1
8 Methyl paraoxybenzoate 0.1
9 Sodium hydroxide 0.05
10 Ethanol 5.0
11 Humus soil extract of Production Example 2
12 Purified water remaining 13 Perfume 0.05

(Production method)
A: Components 7 to 9 are added to component 12 of Example 3 and mixed uniformly at 70 ° C.
B: Components 1 to 6 are mixed uniformly at 70 ° C.
C: B is added to A to emulsify, and cooled to room temperature.
D: Components 10, 11, and 13 were added to C and mixed uniformly to obtain an emulsion.

[Example 4: Liquid foundation (oil-in-water cream)]
(Ingredient) (mass%)
1 Acrylic acid / alkyl methacrylate copolymer (Note 1) 0.5
2 Triethanolamine 1.5
3 Remaining purified water 4 Glycerin 5
5 Ethyl paraoxybenzoate 0.1
6 1,3 Butylene glycol 5
7 Hydrogenated soybean phospholipid 0.5
8 Titanium oxide 5
9 Bengala 0.1
10 Yellow iron oxide 1
11 Black iron oxide 0.05
12 Stearic acid 0.9
13 Glycerol monostearate 0.3
14 cetostearyl alcohol 0.4
15 Polyoxyethylene (20E.O.) sorbitan monooleate 0.2
16 Polyoxyethylene trioleate (20E.O.) sorbitan 0.2
17 2-methoxyhexyl paramethoxycinnamate 5
18 Humus soil extract of Production Example 2
19 Perfume 0.02
(Note 1) Pemulen TR-2 (manufactured by NOVEON)

(Production method)
A: Disperse components 6 to 11 of Example 4 above.
B: Components 12 to 17 are added to A and mixed uniformly at 70 ° C.
C: Components 1 to 5 are mixed uniformly at 70 ° C.
D: B is added to C to emulsify, and cooled to room temperature.
E: Components 18 and 19 were added to D and mixed uniformly to obtain an oil-in-water cream liquid foundation.

[Example 5: Sunscreen cosmetic (water-in-oil cream)]
(Ingredient) (mass%)
1 Polyoxyethylene (20E.O.) sorbitan monolaurate 0.2
2 POE (60) hydrogenated castor oil 0.1
3 Purified water remaining amount 4 Humus soil extract of Production Example 2 5
5 Dipropylene glycol 10
6 Magnesium sulfate 0.5
7 Silicone compound (Note 2) 3
8 Decamethylcyclopentasiloxane 20
9 Isotridecyl isononanoate 5
10 2-Ethylhexyl paramethoxycinnamate 8
11 Stearyl glycyrrhetinate 0.5
12 Dimethylstearyl ammonium hectorite 1.2
(Note 2) KF-6028 (manufactured by Shin-Etsu Chemical Co., Ltd.)

(Production method)
A: Components 1 to 6 in Example 5 are uniformly dispersed.
B: Components 7 to 12 are uniformly dispersed.
C: While stirring B, A was gradually added to emulsify to obtain a water-in-oil cream sunscreen cosmetic.

Claims (2)

A humus soil extract containing 100 mg / L or more of humic substance when converted to an aqueous solution with a solid content concentration of 0.5% by mass as an active ingredient, and
A cosmetic for improving pore conspicuousness, wherein the blending amount of the humus soil extract is 0.02 to 0.04 mass% as a solid content concentration. The cosmetic for improving pore conspicuousness according to claim 1, wherein the humus soil extract is obtained by extraction with one or more selected from water and alcohols.