JP2016204348A - Skin external preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a skin external preparation comprising an amber extract excellent in long-term storage stability at a low temperature.SOLUTION: There is provided a skin external preparation obtained by incorporating one or two or more selected from 0.00001 to 20 mass% of Alpinia pesionsa extracts, 0.00001 to 20 mass% of an artichoke extract and 0.00001 to 20 mass% of a yeast extract in a skin external preparation comprising 0.000001 to 0.1 mass% of an amber extract. The extracts of the leaf portions of Alpinia speciosa and artichoke are preferably used.

Description

The present invention relates to a skin external preparation containing a wrinkle extract.

The cocoon is a fossil that is mainly formed by the resin of a pine genus plant buried in the basement for a long time and condensed, and mainly contains resin, essential oil, succinic acid and the like. It is soluble in a small amount in ethanol, diethyl ether or benzene (see Non-Patent Document 1, for example). In the past, the casket itself was used for ornamental crafts, jewelry, insulating materials, and shavings for incense, and in the 19th century it was also used for medical care such as wounds (for example, Non-patent document 2).

In recent years, techniques for blending wrinkle powder into cosmetics to improve skin feel (see, for example, Patent Document 1), techniques for blending wrinkle extract into skin external preparations (for example, see Patent Document 2, Patent Document 3) ), Technology utilizing the whitening effect of hot water extract (see, for example, Patent Literature 4 and Patent Literature 5), technology utilizing a skin turnover promoting factor in the salmon extract fraction (eg, Patent Literature 6) (See, for example, Patent Document 7), Technology for utilizing hyaluronic acid production promoting factor in the salmon extract fraction (see, for example, Patent Document 8) ), A technique using an angiogenesis-promoting factor in the sputum extract fraction (for example, see Patent Document 9) and the like have been reported.

As described above, various physicochemical and biological effects are known for cocoon, and it has been used in various fields as a very useful material and is expected to be a material with infinite possibilities. Yes.

Among these, the field to be used in daily life is skin external preparations such as cosmetics, but cosmetics are blended with various ingredients to make products in a wide variety of dosage forms, so that touch and effect are exhibited. It is necessary that the blended components exist stably. However, the external preparation for skin containing the wrinkle extract has a problem in stability during long-term storage at a low temperature. Furthermore, a skin external preparation containing at least one selected from a cocoon extract, a moon peach extract, an artichoke extract and a yeast extract is not known at all.

  JP 2004-83478 A   JP H9-227334   JP 2001-131048 A   JP 2010-235551 A   JP 2012-240967 A   JP 2007-314522 A   JP 2008-189669 A   JP 2008-266260 A   JP2011-256164A

  Chuyaku University Dictionary Vol.2 Shanghai Science and Technology Publishers (Jiangsu New Medical School “Chugaku Daikichi” Editorial Department) Shogakukan) Reference 1   K. Kaisering Pathology, 22 (4), 285-286 (2001)

This invention makes it a subject to provide the skin external preparation containing the wrinkle extract excellent in long-term stability at low temperature.

In view of such a situation, as a result of intensive research, the present inventors have found that a skin external preparation containing a koji extract contains one or more of a moon peach extract, an artichoke extract, and a yeast extract. Thus, the present invention has been completed by finding a skin external preparation with excellent stability that does not cause precipitation derived from wrinkle extract during long-term storage at a low temperature. That is, the skin external preparation of the present invention is as follows.
(1) A skin external preparation, comprising one or more selected from a strawberry extract, a moon peach extract, an artichoke extract, and a yeast extract.
(2) The external preparation for skin according to (1), comprising 0.000001 to 0.1% by mass of the wrinkle extract.
(3) The external preparation for skin according to (1) or (2), characterized by containing 0.00001% by mass to 20% by mass of the moon peach extract.
(4) The external preparation for skin according to any one of (1) to (3), which contains 0.00001% by mass to 20% by mass of the artichoke extract.
(5) The skin external preparation according to any one of (1) to (4), wherein the yeast extract contains 0.00001% by mass to 20% by mass.
(6) The external preparation for skin according to any one of (1) to (5), which is a cosmetic.

ADVANTAGE OF THE INVENTION According to this invention, the skin external preparation containing the wrinkle extract excellent in stability which does not generate | occur | produce precipitation by long-term storage at low temperature can be provided.

(1) Wrinkle extract of the present invention The skin external preparation of the present invention is characterized by containing a wrinkle extract as an essential component. Here, the cocoon extract as used in the present invention is the cocoon itself, a processed product obtained by pulverizing or chopping the cocoon, an extract obtained by adding a solvent to the cocoon or the processed product, and an extraction obtained by removing the solvent from the extract. Examples thereof include a solvent-removed product of the product, a purified product thereof, and the like. Among these, the extract or the solvent-removed product thereof is particularly preferable.

Examples of the solvent for the extract include water, alcohol such as methanol, ethanol, 1,3-butanediol, propylene glycol, and glycerin, esters such as ethyl acetate and methyl formate, nitriles such as acetonitrile, diethyl ether, Examples include ethers such as tetrahydrofuran, halogenated hydrocarbons such as chloroform and methylene chloride, ketones such as acetone and methyl ethyl ketone, and these may be used alone or in combination. Most preferred among these are water or alcohols.

For example, the extraction may be carried out by adding 2 to 10 times the amount of solvent to the pulverized koji and immersing it for several days at room temperature and for several hours at a temperature near the boiling point. Thereafter, insoluble matters may be removed by filtration or the like, followed by concentration under reduced pressure. Alternatively, it may be purified by column chromatography on a column packed with silica gel, octadecylsilylated silica gel, ion exchange resin or the like.

<Production Example 1>
The soot is pulverized to an appropriate size so that the solvent can be easily extracted. As the pulverizing means, a file or a jet mill pulverizer can be used. Further, it is also possible to use a material obtained by pulverizing chips produced when processing a jar as a gemstone. The size to be pulverized is not particularly limited. From the viewpoint of extraction efficiency with a solvent, for example, the average particle size can be up to 100 μm, preferably about 10 to 30 μm. The ground soot is washed with a hydrophobic organic solvent as necessary. As the hydrophobic organic solvent, hexane, benzene, chloroform or a mixture thereof can be used. This washing can be omitted.
As the extraction step, the soot or the soot that has been crushed, ground and washed in the pretreatment step can be immersed in a lower alcohol to obtain an extract. For example, the cocoon or the crushed or ground and washed cocoon in the pretreatment step is immersed in a lower alcohol for a long period of 7 days or more at a low temperature or at room temperature to obtain an extract. Specifically, the extract is obtained by immersing the cocoon or the crushed or ground and washed cocoon in the pretreatment step in a lower alcohol at a temperature of 25 ° C. to 50 ° C. under normal pressure for 7 days or more. obtain. More specifically, for example, in the case of ethanol, the soot or the soot that has been crushed or ground and washed in the pretreatment step is at least 7 days at a temperature of 25 ° C. to 50 ° C., preferably about 40 ° C. An extract is obtained by soaking for one month (30 days). The extract is concentrated and dried to a brownish candy-like dried product. As the lower alcohol, methanol, ethanol, butanol or a mixture thereof can be used.
As the fractionation step, the obtained extract is subjected to column chromatography using a lower alcohol, for example, methanol, as a solvent, and a hydrocarbon chemically bonded silica gel, for example, octadecylsilylated silica gel, as a carrier, to obtain an eluate. It can be fractionated according to the color.
Furthermore, purification by adsorption column chromatography can also be performed. Specifically, column chromatography using silica gel as a carrier can be used as the adsorption column chromatography. As an elution solvent, for example, it is possible to elute with a mixed solution of benzene and ethyl acetate as a solvent, specifically, benzene: ethyl acetate = 50: 1.

<Production Example 2>
The lower alcohol extract (dried product) of koji obtained in Production Example 1 can be further fractionated into two phases, an aqueous phase and an organic solvent phase, using an appropriate organic solvent. Examples of the organic solvent include lower ethers, specifically, dimethyl ether or diethyl ether. The obtained lower alcohol extract of koji is dissolved in the organic solvent, and undissolved components are removed. Next, the organic solvent in which the soot extract is dissolved is washed with water. Specifically, pure water, for example, ultrapure water produced by an ultrapure water production apparatus is added to remove water-soluble components. If necessary, the removal of the water-soluble component using pure water is repeated 3 to 5 times.
Thereafter, an acidic aqueous solution and a basic aqueous solution are added several times as necessary, and mixed until the basic water-soluble component and the acidic water-soluble component are finally removed from the coloring component, respectively. ,Remove. It is desirable to wash 3 to 5 times with an acidic aqueous solution, and then wash several times with a basic aqueous solution until the organic solvent in which the koji extract is dissolved is removed. As the acidic aqueous solution, for example, dilute hydrochloric acid having a pH of 3.0, and as the basic aqueous solution, for example, a sodium hydroxide aqueous solution having a pH of 12.0 can be used. Finally, wash with pure water.

<Production Example 3>
The organic solvent in which the koji extract after removal of the water-soluble component in Production Example 2 is dissolved is first washed with an acidic aqueous solution as necessary. Although the cleaning with the acidic aqueous solution can be omitted, it is desirable to perform the cleaning first with the acidic cleaning solution. Next, it is washed with a basic aqueous solution. Collect the basic aqueous solution after washing. Further, when the organic solvent is finally washed with pure water, this washing solution can also be added to the basic aqueous solution after washing. Then, after washing, the basic aqueous solution is neutralized with an acid, and if necessary, a salt is added until it is saturated. The salt added here may be the same salt as the salt formed by the basic aqueous solution used in the washing and the acid added for neutralization. For example, when a sodium hydroxide aqueous solution is used as the basic aqueous solution and hydrochloric acid is used for neutralization, sodium chloride can be added as a salt to saturation. After being saturated with a salt, back extraction with an organic solvent is performed, and the organic solvent is volatilized to obtain an acidic fraction. Examples of the organic solvent used for back extraction include lower ether, dichloromethane, benzene and the like, and specifically, dimethyl ether and diethyl ether.
Any acidic aqueous solution can be used as the acidic aqueous solution, and examples thereof include hydrochloric acid, an aqueous citric acid solution, and acetic acid, and more specifically, diluted hydrochloric acid having a pH of 3.0. As the basic aqueous solution, any basic aqueous solution can be used. For example, sodium hydroxide aqueous solution, sodium hydrogen carbonate aqueous solution, calcium hydroxide aqueous solution, more specifically, sodium hydroxide having a pH of 12.0 An aqueous solution can be used.

By containing the wrinkle extract of the present invention in an external preparation for skin, it is excellent in skin beautifying effects such as skin dullness improvement, drying prevention, moisturizing, wrinkle prevention, whitening and hair growth effect. The preferable content of the wrinkle extract in the external preparation for skin of the present invention is 0.000001 to 0.1% by mass, more preferably 0.00001% by mass to solid content with respect to the total amount of external preparation for skin. 0.01% by mass. This is because if the amount is too small, the skin beautifying effect may not be exhibited, and if the amount is too large, the effect may reach its peak and the degree of freedom of other prescription ingredients may be impaired.

(2) Moon peach extract of the present invention The topical skin preparation of the present invention is characterized by containing an extract of Alpinia speciosa Wendl. K. Schum. As an essential component. Here, the moon peach extract referred to in the present invention is a moon peach or its dried product itself, a processed product obtained by pulverizing, shredding, etc. An extract obtained by adding a solvent to the extract, a solvent removed product of the extract obtained by removing the solvent from the extract, a purified product thereof, and the like can be exemplified. Among these, the extract or the solvent removed product thereof is particularly preferable. The plant part to be used is particularly preferably a leaf part. This is because the stability of the external preparation for skin of the present invention during long-term storage at low temperatures is maximized. The extraction is preferably performed by adding 1 to 30 parts by mass of a solvent to 1 part by mass of moon peach or its dried product and immersing at room temperature for 24 hours or more, or refluxing for 1 to 5 hours or more. The extraction solvent is preferably a polar solvent, one or two selected from water, alcohols such as methanol, ethanol, isopropyl alcohol and butanol, ketones such as acetone and methyl ethyl ketone, and ethers such as diethyl ether and tetrahydrofuran. A seed | species or more can be illustrated suitably, 30-100 mass%, More preferably, 70-100 mass% ethanol containing aqueous solution and 80 mass% ethanol can be illustrated especially suitably. After soaking, insolubles can be removed by filtration, and then the solvent can be removed by vacuum concentration or the like, but removal by freeze-drying is particularly preferred. The powder composition from which the solvent has been removed may be used as it is, but after that, liquid-liquid extraction with water and ethyl acetate, water and butanol, or column chromatography packed with silica gel or ion exchange resin. It may be fractionated and purified by the same method. The preferable content of the moon peach extract in the external preparation for skin of the present invention is 0.00001% by mass to 20% by mass, more preferably 0.0001% by mass to 2 as solid content with respect to the total amount of external preparation for skin. It is preferable to be contained by mass%. The moon peach extract thus obtained is derived from the koji extract in the long-term storage of the skin external preparation of the present invention at a low temperature alone, more preferably in a combination of the postscript artichoke extract and the postscript yeast extract. It has the effect of being excellent in stability without causing precipitation or scum.

<Production Example 4>
Weighed 200 g of dry matter from the leaves of Alpinia speciosa Wendl. K. Schum., Pulverized, added 21 80% by weight ethanol aqueous solution, refluxed for 2 hours, and removed insolubles by filtration. After that, the filtrate was concentrated and freeze-dried to obtain 20.2 g of extract.

(3) Artichoke extract of the present invention The topical skin preparation of the present invention is characterized in that it contains an extract of the asteraceae artichoke (also known as Cynara scolimus L. (Compositae)) as an essential component. Here, the artichoke extract referred to in the present invention is an artichoke or a dried product itself, a processed product obtained by pulverizing or chopping the artichoke or the dried product, an artichoke or a dried product thereof, or a solvent added to the processed product. The extracted extract, the solvent removed product of the extract obtained by removing the solvent from the extract, the purified product thereof and the like can be exemplified. Among these, the extract or the solvent removed product thereof is particularly preferable. The plant part to be used is particularly preferably a leaf part. This is because the stability of the external preparation for skin of the present invention during long-term storage at low temperatures is maximized. The extraction is preferably performed by adding 1 to 30 parts by mass of a solvent to 1 part by mass of artichoke or a dried product thereof and immersing at room temperature for 24 hours or more, or refluxing for 1 to 5 hours or more. The extraction solvent is preferably a polar solvent, one or two selected from water, alcohols such as methanol, ethanol, isopropyl alcohol and butanol, ketones such as acetone and methyl ethyl ketone, and ethers such as diethyl ether and tetrahydrofuran. A seed | species or more can be illustrated suitably, 30-100 mass%, More preferably, 70-100 mass% ethanol containing aqueous solution and 100 mass% ethanol can be illustrated especially suitably. After soaking, insolubles can be removed by filtration, and then the solvent can be removed by vacuum concentration or the like, but removal by freeze-drying is particularly preferred. The powder composition from which the solvent has been removed may be used as it is, but after that, liquid-liquid extraction with water and ethyl acetate, water and butanol, or column chromatography packed with silica gel or ion exchange resin. It may be fractionated and purified by the same method. The preferable content of the artichoke extract in the external preparation for skin of the present invention is 0.00001% by mass to 20% by mass, more preferably 0.0001% by mass to 2% by mass as a solid content with respect to the total amount of the external preparation for skin. % Content is preferable. The artichoke extract thus obtained alone is more preferably derived from the koji extract in the long-term storage of the skin external preparation of the present invention at a low temperature in the combination of the moon peach extract and the yeast extract described later. It has the effect of being excellent in stability without causing precipitation or scum.

<Production Example 5>
After weighing 200 g of dry matter of the leaf part of the asteraceae artichoke (Cynara school) (Compositae), pulverizing it, adding 21 ethanol, refluxing for 2 hours, removing insoluble matter by filtration, Concentration and lyophilization gave 15.6 g of extract.

(4) Yeast extract of the present invention The external preparation for skin of the present invention is characterized by containing an extract of yeast (Saccharomyces cerevisiae) as an essential component. Here, the yeast extract referred to in the present invention is a yeast or a dried product itself, a processed product obtained by homogenizing the yeast or the dried product, an extract obtained by adding a solvent to the yeast or the dried product, or the processed product. Products, extracts obtained by removing the solvent from the extract, solvent-purified products thereof, and purified products thereof. Among these, those obtained by concentrating or drying a digestion solution containing amino acids, saccharides and the like obtained by self-digestion of yeast itself are particularly preferable. This can also be purchased and used as a “hydrolyzed yeast extract” which is commercially available, for example, from Maruzen Pharmaceutical Co., Ltd. The preferable content of the yeast extract in the external preparation for skin of the present invention is 0.00001% by mass to 20% by mass, more preferably 0.0001% by mass to 2% by mass as the solid content with respect to the total amount of the external preparation for skin. % Content is preferable. The yeast extract thus obtained alone, more preferably in combination with the moon peach extract and the yeast extract, is derived from the koji extract in long-term storage of the skin external preparation of the present invention at a low temperature. It has the effect of being excellent in stability without causing precipitation or scum.

(5) External preparation for skin of the present invention The external preparation for skin of the present invention can be used without particular limitation as long as it is applied externally to the skin. For example, cosmetics, external preparations for skin, external use for skin Goods and the like can be suitably exemplified, and it is particularly preferable to apply to cosmetics. It can be produced in various forms such as liquid, paste, cream, ointment, powder, and patch. In addition, other commonly added components such as mineral oils, higher alcohols, animal and vegetable oils, waxes, silicone oils, moisturizers, wetting agents, water-soluble polymers, lower alcohols, water, antioxidants, pH Modifiers, dyes, pigments, antiseptic fungicides, anti-inflammatory agents and other medicinal agents, chelating agents, and the like can also be added. The content ratio of one or more selected from the moon peach extract, artichoke extract and yeast extract and the koji extract is preferably 10: 1 to 200: 1 in terms of mass ratio. 1 to 50: 1 is more preferable. This is because in this range, the external preparation for skin of the present invention exhibits an excellent effect on long-term stability at low temperatures.

The cosmetic of the present invention is not limited in its dosage form, and examples include dosage forms such as lotions, emulsions, creams, ointments, solutions, gels, packs, lotions, powders, sticks and the like.

The cosmetics of the present invention can appropriately contain other additive components that are usually used as cosmetic raw materials. Further, the cosmetic of the present invention may contain other base components that are usually used as cosmetic raw materials. Base ingredients include, for example, liquid oils (olive oil, etc.), solid oils (sheafa, etc.), waxes (beeswax, etc.), hydrocarbon oils (liquid paraffin, paraffin, petrolatum, etc.), higher fatty acids (stearic acid, etc.) , Oily components such as higher alcohols (such as cetanol), synthetic ester oils (such as octyldodecyl myristate), silicones (such as methylpolysiloxane), various surfactants, sequestering agents, water-soluble polymers (carboxyvinyl) Polymer, etc.), thickeners, various powder components, fragrances, water and the like.

Hereinafter, the present invention will be described in more detail with reference to examples. However, it is needless to say that the present invention is not limited to these examples.

<Examples 1 to 10: lotion>
According to the prescription of Table 1 shown below, as Examples 1-10, the skin lotion and the comparative examples 1-3 which are the skin external preparations of this invention were produced. About these, the external appearance observation (4 degreeC state and normal temperature return | restoration, the state after stirring) was implemented after one month preservation | save at 4 degreeC. The observation results are shown in the lower part of Table 1. From the observation results, it can be seen that the lotion of the present invention has excellent stability with respect to long-term storage at low temperatures.

<Examples 11 to 20: O / W cream>
According to the prescription of Table 2 shown below, as Examples 11-20, the O / W cream and Comparative Examples 4-6 which are external preparations of this invention were produced. That is, the components of (a) and (b) were heated to 80 ° C., and (b) was mixed with stirring while being stirred and cooled to obtain the O / W cream of the present invention. About these, the state (presence or absence) of the contents after 1 to 4 months of storage at 4 ° C. was visually observed. The observation results are shown in the lower part of Table 2. From the observation results, it can be seen that the O / W cream of the present invention has excellent stability with respect to long-term storage at low temperatures.

Claims (6)

A skin external preparation containing a strawberry extract, further comprising one or more selected from a moon peach extract, an artichoke extract and a yeast extract. The external preparation for skin according to claim 1, comprising 0.000001% by mass to 0.1% by mass of the wrinkle extract. The external preparation for skin according to claim 1 or 2, characterized in that it contains 0.00001% by mass to 20% by mass of the moon peach extract. The external preparation for skin according to any one of claims 1 to 3, comprising 0.00001% by mass to 20% by mass of the artichoke extract. The skin external preparation according to any one of claims 1 to 4, wherein the yeast extract contains 0.00001% by mass to 20% by mass. It is cosmetics, The skin external preparation in any one of Claims 1-5 characterized by the above-mentioned.