JP6118262B2 - 乾燥試料から遺伝子材料を電気溶出する方法 - Google Patents
乾燥試料から遺伝子材料を電気溶出する方法 Download PDFInfo
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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- Biomedical Technology (AREA)
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Description
Claims (23)
- 固形媒体上に保存された生物学的試料から遺伝子材料を抽出する方法であって、
生物学的試料を溶解して遺伝子材料を保持する化学物質であってタンパク質変成剤及びキレート剤を含む化学物質とセルロース系の紙とを含む固形媒体上に生物学的試料がアプライされた固形媒体を取得すること、及び
遺伝子材料を固形媒体から次の媒体へ直接電気溶出すること
を含む、方法。 - 電気溶出の前に固形媒体をすすぐことを含む、請求項1記載の方法。
- 固形媒体のすすぎが固形媒体を緩衝液中に浸漬すること又は固形媒体を通して又はその表面に緩衝液を流すことを含む、請求項2記載の方法。
- 固形媒体のすすぎが固形媒体を水中に浸漬すること又は固形媒体を通して又はその表面に水を流すことを含む、請求項2記載の方法。
- 電気溶出の前に遺伝子材料を修復することをさらに含む、請求項2記載の方法。
- 遺伝子材料の修復がDNAポリメラーゼ及びDNAリガーゼを少なくとも含有する溶液をアプライすることを含む、請求項5記載の方法。
- DNAポリメラーゼがDNAポリメラーゼ活性、プルーフリーディングのための3’→5’エキソヌクレアーゼ活性及びニックトランスレーションのための5’→3’エキソヌクレアーゼ活性を含む、請求項6記載の方法。
- 遺伝子材料の修復が、脱塩基部位に隣接するDNAにニックを入れるエンドヌクレアーゼを少なくとも含有する溶液をアプライすることを含む、請求項5記載の方法。
- 生物学的試料が、固定化細胞を含む、請求項1記載の方法。
- 固形媒体から電気溶出する前に固定化細胞を処理することを含み、処理が固定化細胞を再水和すること、固定化細胞をプロテアーゼ処理すること又は脱架橋することを含む、請求項9記載の方法。
- 次の媒体が電気泳動ゲル、溶液又は捕捉表面を含む、請求項1記載の方法。
- 固形媒体上に保存された固定化組織試料から遺伝子材料を抽出する方法であって、
組織試料を溶解して遺伝子材料を保持することができる化学物質であってタンパク質変成剤及びキレート剤を含む化学物質とセルロース系の紙とを含む固形媒体上に、固定化組織試料の少なくとも一部をアプライすること、及び
遺伝子材料を固形媒体から次の媒体へ直接電気溶出すること
を含む、方法。 - 固形媒体がFTA(登録商標)紙を含む、請求項12記載の方法。
- 固定化組織試料を固形媒体にアプライする前又は後に固定化組織試料をプロテアーゼで処理することを含む、請求項12記載の方法。
- 固形媒体が固定化組織試料を溶解する化学物質を含む、請求項12記載の方法。
- 固定化組織試料を固形媒体にアプライする前に固定化組織試料を再水和することを含む、請求項12記載の方法。
- 固定化組織試料を固形媒体にアプライする前に又はその後に遺伝子材料とタンパク質との間を脱架橋することを含む、請求項16記載の方法。
- 脱架橋が求核試薬を含むpHが7.0以外の架橋修復緩衝液をアプライすることを含む、請求項17記載の方法。
- 電気溶出の前に遺伝子材料を修復することを含む、請求項12記載の方法。
- 遺伝子材料の修復がDNAポリメラーゼI及びDNAリガーゼを少なくとも含有する溶液をアプライすることを含む、請求項19記載の方法。
- 遺伝子材料の修復が、脱塩基部位に隣接するDNAにニックを入れるエンドヌクレアーゼを少なくとも含有する溶液をアプライすることを含む、請求項19記載の方法。
- 次の媒体が電気泳動ゲル、溶液又は捕捉表面を含む、請求項12記載の方法。
- セルロース系紙上に保存された生物学的試料からDNAを抽出する方法であって、
生物学的試料を溶解してDNAを保持する化学物質であってタンパク質変成剤及びキレート剤を含む化学物質を含むセルロース系紙上に生物学的試料がアプライされたセルロース系紙を取得すること、
セルロース系紙上でDNAを修復すること、及び
DNAをセルロース系紙から電気泳動ゲル中へ直接電気溶出すること
を含む、方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/972,236 US20120152743A1 (en) | 2010-12-17 | 2010-12-17 | Method for electroeluting genetic material from dried samples |
US12/972,236 | 2010-12-17 | ||
PCT/US2011/064823 WO2012082849A1 (en) | 2010-12-17 | 2011-12-14 | Method for electroeluting genetic material from dried samples |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2014502495A JP2014502495A (ja) | 2014-02-03 |
JP2014502495A5 JP2014502495A5 (ja) | 2016-04-28 |
JP6118262B2 true JP6118262B2 (ja) | 2017-04-19 |
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Application Number | Title | Priority Date | Filing Date |
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JP2013544728A Active JP6118262B2 (ja) | 2010-12-17 | 2011-12-14 | 乾燥試料から遺伝子材料を電気溶出する方法 |
Country Status (4)
Country | Link |
---|---|
US (1) | US20120152743A1 (ja) |
EP (1) | EP2652175B1 (ja) |
JP (1) | JP6118262B2 (ja) |
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US9480966B2 (en) | 2012-04-30 | 2016-11-01 | General Electric Company | Substrates and methods for collection, stabilization and elution of biomolecules |
US9040679B2 (en) | 2012-04-30 | 2015-05-26 | General Electric Company | Methods and compositions for extraction and storage of nucleic acids |
US9040675B2 (en) | 2012-04-30 | 2015-05-26 | General Electric Company | Formulations for nucleic acid stabilization on solid substrates |
US9044738B2 (en) | 2012-04-30 | 2015-06-02 | General Electric Company | Methods and compositions for extraction and storage of nucleic acids |
US9399986B2 (en) | 2012-07-31 | 2016-07-26 | General Electric Company | Devices and systems for isolating biomolecules and associated methods thereof |
GB2552110B (en) * | 2012-08-30 | 2018-05-23 | Gen Electric | Methods of isolating nucleic acids under reduced degradation condition |
US9999856B2 (en) | 2013-07-26 | 2018-06-19 | General Electric Company | Methods for electroelution of biomolecules |
US9333463B2 (en) | 2013-07-26 | 2016-05-10 | General Electric Company | Devices and systems for elution of biomolecules |
CN108473932B (zh) | 2015-09-09 | 2022-07-15 | 集联健康有限公司 | 用于样品收集、稳定化和保存的系统、方法和装置 |
JP2018134392A (ja) | 2017-01-10 | 2018-08-30 | ドローブリッジ ヘルス,インコーポレイテッド | サンプル収集のためのデバイス、システム、及び方法 |
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US5187083A (en) * | 1990-11-13 | 1993-02-16 | Specialty Laboratories, Inc. | Rapid purification of DNA |
FR2726286B1 (fr) * | 1994-10-28 | 1997-01-17 | Genset Sa | Procede d'amplification d'acides nucleiques en phase solide et trousse de reactifs utile pour la mise en oeuvre de ce procede |
AR021833A1 (es) * | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
US6221635B1 (en) * | 1999-05-06 | 2001-04-24 | The Wistar Institute | Methods for solid-phase amplification of DNA template (SPADT) using multiarrays |
JP4071907B2 (ja) * | 1999-11-29 | 2008-04-02 | オリンパス株式会社 | 自動核酸検査装置 |
US6503716B1 (en) * | 2000-11-28 | 2003-01-07 | Pe Corporation (Ny) | Compositions and methods for extracting a nucleic acid |
US20040219545A1 (en) * | 2001-04-13 | 2004-11-04 | Rando Robert F. | Methods for identifying small molecules that bind specific rna structural motifs |
WO2002083901A2 (en) * | 2001-04-12 | 2002-10-24 | Cropdesign N.V. | Plant growth regulating genes, proteins and uses thereof |
GB0127809D0 (en) * | 2001-11-20 | 2002-01-09 | Dna Res Innovations Ltd | Purification of nucleic acid |
JP5441290B2 (ja) * | 2002-10-04 | 2014-03-12 | ジーイー・ヘルスケア・バイオサイエンス・コーポレイション | 核酸精製システムの媒体上に核酸を保存するツールとして、化学物質を用いる方法および材料 |
JP2006506057A (ja) * | 2002-10-07 | 2006-02-23 | マーリゲン、バイオサイエンシーズ、インコーポレーテッド | 生物学的試料からdnaの抽出 |
US20040241734A1 (en) * | 2003-04-28 | 2004-12-02 | Exagen Diagnostics | Methods for in situ hybridization without the need for competitior DNA |
EP1633893B1 (en) * | 2003-05-19 | 2012-01-25 | Gen-Probe Incorporated | Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample |
KR20070073917A (ko) * | 2004-10-21 | 2007-07-10 | 뉴 잉글랜드 바이오랩스, 인크 | 개선된 증폭을 위한 핵산 복구 |
US7727718B2 (en) * | 2005-01-04 | 2010-06-01 | Molecular Research Center, Inc. | Reagents for storage and preparation of samples for DNA analysis |
US20120109530A1 (en) * | 2005-06-16 | 2012-05-03 | Parks Patrick J | Method of classifying chemically crosslinked cellular samples using mass spectra |
GB0604973D0 (en) * | 2006-03-11 | 2006-04-19 | Central Science Lab Csl Of San | Purification method and kit |
EP2394152B1 (en) * | 2009-02-03 | 2019-05-01 | ANDE Corporation | Nucleic acid purification |
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US20120152743A1 (en) | 2012-06-21 |
EP2652175B1 (en) | 2017-09-20 |
WO2012082849A1 (en) | 2012-06-21 |
EP2652175A1 (en) | 2013-10-23 |
EP2652175A4 (en) | 2014-05-21 |
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