JP6117773B2 - 音響出射およびサンプル保存に適合した、液体サンプルを保持し移動させるための方法 - Google Patents
音響出射およびサンプル保存に適合した、液体サンプルを保持し移動させるための方法 Download PDFInfo
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
- B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
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Description
本願は、同一出願人による、2011年4月28日に出願された米国仮出願61/479,985号の優先権を主張し、本願において、ここでの参照のために導入される。
本発明はサンプルの取り扱いに関する。特に、本発明のある実施形態では、音響出射および分析に適合したサンプル容器ならびその方法を記載している。例示として本発明は生体サンプルに適用されているが、サンプル容器内の液体サンプルの保存などその適用範囲はずっと広いものであることが認識されるだろう。
本発明はサンプルの取り扱いに関する。特に、本発明のある実施形態では、音響出射および分析に適合したサンプル容器ならびにその方法を記載している。例示として本発明は生体サンプルに適用されているが、サンプル容器内の液体サンプルの保存などその適用範囲はずっと広いものであると認識されるだろう。
図1(A)及び(B)は、本発明の実施形態に係る音響出射および分析に適合したサンプル容器を示す概略図である。
本発明はサンプルの取り扱いに関する。特に、本発明のある実施形態では、音響出射および分析に適合したサンプル容器ならびにその方法を記載している。例示として本発明は生体サンプルに適用されているが、サンプル容器内の液体サンプルの保存などその適用範囲はより広いものであると認識されるだろう。
実施例Aを図9に示すように実施形態として行った。ポリプロピレン1.5mLマイクロチューブ(Eppendorf、Safe−Lock、カタログ番号022363204、Hauppauge、ニューヨーク)をPVA TePla America(コロナ、CA)へ送り、そこでこれらのチューブをプラズマで処理し当該チューブの露出した表面を共有結合されたヒドロキシル基の導入により親水性にした。
実施例Bを図9に示すように別の実施形態として行った。ポリプロピレン1.5mLマイクロチューブをIntegrated Surface Technologies(Menlo Park、CA)へ送り、そこでこれらのチューブを処理し当該チューブの露出した表面をシリカの原子層成長法により親水性にした。
液体サンプルがサンプル容器に入るように構成された注入口と、
上記液体サンプルの1以上の液滴のそれぞれが、1以上の音響出射のそれぞれによって、サンプル容器から排出されるように構成された排出口と、を備え、
上記注入口および排出口は異なる位置にあることを特徴とするサンプル容器。
注入口を介して液体サンプルをサンプル容器に移動させる工程と、
排出口を介して上記液体サンプルの1以上の液滴をサンプル容器から音響出射させる工程と、を含み、
上記注入口および排出口は異なる位置にあることを特徴とする方法。
少なくとも上記側面を介して上記サンプル容器と音響発生器とを音響結合させる工程と、
上記音響発生器により音響ビームを発生させる工程と、
上記音響ビームを上記液体サンプル中の焦点へ合焦させる工程と、をさらに含むことを特徴とする(12)に記載の方法。
上記液体サンプルから上記1以上の気泡を除去する工程と、をさらに含むことを特徴とする(12)に記載の方法。
上記液体サンプルを複数の成分に階層化する工程と、を含み、
上記1以上のフロートのそれぞれは、上記複数の成分の内の1以上の成分に対応する濃度と関連付けられていることを特徴とする(12)に記載の方法。
上記排出口を介して上記液体サンプルの1以上の液滴をサンプル容器から音響出射させる工程は、上記1以上のフロートの少なくとも1つに対応する上記1以上の成分の少なくとも1つの液滴を音響出射させる工程を含むことを特徴とする(17)に記載の方法。
上記サンプル容器内にある上記1以上のフロートの各々の1以上の位置を決定する工程と、をさらに含むことを特徴とする(12)に記載の方法。
上記1以上の位置の1つと関連付けられた情報に少なくとも基づき、上記音響発生器を上記1以上のフロートの少なくとも1つに位置合わせする工程と、
上記音響発生器により音響ビームを発生させる工程と、
上記液体サンプル内の上記1以上のフロートの1つに対応する焦点に上記音響ビームを合焦させる工程と、を含むことを特徴とする(23)に記載の方法。
液体サンプルをサンプル容器に移動させる工程と、
上記サンプル容器の1以上の内面に1以上の物質を添加する工程と、
上記サンプル容器の上記1以上の内面上の上記1以上の物質に、上記液体サンプルの1以上の成分を結合させる工程と、を含むことを特徴とする方法。
Claims (13)
- 液体サンプルを保持し移動させるための方法であって、
注入口を介して液体サンプルをサンプル容器に移動させる工程と、
上記サンプル容器へ1以上のフロートを配置する工程と、
上記液体サンプルを複数の成分に階層化する工程と、
排出口を介して上記液体サンプルの1以上の液滴をサンプル容器から音響出射させる工程と、を含み、
上記注入口および排出口は異なる位置にあり、
上記1以上のフロートのそれぞれのフロートは、上記複数の成分の内の1以上の成分に対応する濃度と関連付けられていることを特徴とする方法。 - 上記サンプル容器における長軸に沿った側面を、長軸の方向が水平方向となる横向きに配置する工程と、
少なくとも上記側面を介して上記サンプル容器と音響発生器とを音響結合させる工程と、
上記音響発生器により音響ビームを発生させる工程と、
上記音響ビームを上記液体サンプル中の焦点へ合焦させる工程と、をさらに含むことを特徴とする請求項1に記載の方法。 - 上記排出口を介して上記液体サンプルの1以上の液滴をサンプル容器から音響出射させる工程は、上記側面の反対側の上記排出口を介して上記1以上の液滴を音響出射させる工程を含むことを特徴とする請求項2に記載の方法。
- 上記サンプル容器を少なくとも揺動または回転させることによって、上記液体サンプルから1以上の気泡を除去する工程をさらに含むことを特徴とする請求項1に記載の方法。
- 音響により上記液体サンプル中の1以上の気泡を検出する工程と、
上記液体サンプルから上記1以上の気泡を除去する工程と、をさらに含むことを特徴とする請求項1に記載の方法。 - 上記1以上のフロートのそれぞれのフロートが上記複数の成分の内の1以上の成分を選択された収集領域に保持した状態で、上記サンプル容器を、長軸の方向が鉛直方向となる縦向きから長軸の方向が水平方向となる横向きに変える工程をさらに含むことを特徴とする請求項1に記載の方法。
- 上記1以上のフロートうち少なくとも1つのフロートの位置を音響的に決定する工程をさらに含み、
上記排出口を介して上記液体サンプルの1以上の液滴をサンプル容器から音響出射させる工程は、上記1以上のフロートのうち少なくとも1つのフロートに対応する上記1以上の成分の少なくとも1つの液滴を音響出射させる工程を含むことを特徴とする請求項1に記載の方法。 - 1以上の化学反応を起こし、上記液体サンプルにおける1以上の検体の劣化または分解を低減させるために、上記液体サンプルに1以上の物質を添加することにより上記サンプル容器を修飾する工程をさらに含むことを特徴とする請求項1に記載の方法。
- 上記サンプル容器を修飾する工程は、上記液体サンプルの1以上のサンプル成分をトラップまたは不活性化する工程を含むことを特徴とする請求項8に記載の方法。
- 上記サンプル容器を修飾する工程において、上記1以上の出射された液滴へ上記1以上の物質のいずれも放出しないことを特徴とする請求項8に記載の方法。
- 上記サンプル容器における長軸に沿った側面を、長軸の方向が水平方向となる横向きに配置する工程と、
上記サンプル容器内にある上記1以上のフロートの各々の1以上の位置を決定する工程と、をさらに含むことを特徴とする請求項1に記載の方法。 - 上記1以上のフロートの各々の1以上の位置を決定する工程は、上記1以上のフロートを音響的に検出する工程を含むことを特徴とする請求項11に記載の方法。
- 少なくとも上記側面を介して上記サンプル容器と音響発生器とを音響結合させる工程と、
上記1以上の位置のうち1つの位置と関連付けられた情報に少なくとも基づき、上記音響発生器を上記1以上のフロートのうち少なくとも1つのフロートに位置合わせする工程と、
上記音響発生器により音響ビームを発生させる工程と、
上記液体サンプル内の上記1以上のフロートのうち1つのフロートに対応する焦点に上記音響ビームを合焦させる工程と、を含むことを特徴とする請求項11に記載の方法。
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US10592793B2 (en) * | 2014-01-14 | 2020-03-17 | Labcyte Inc. | Sample containers having identification marks embedded therein and being adapted for acoustic ejections |
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DE102014105219A1 (de) * | 2014-04-11 | 2015-10-15 | Plasma Electronic Gmbh | Analysebehältnis sowie Analysesystem |
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CA3127191A1 (en) | 2019-01-21 | 2020-07-30 | Eclipse Medcorp, Llc | Methods, systems and apparatus for separating components of a biological sample |
US11559613B2 (en) | 2019-02-06 | 2023-01-24 | Hanuman Pelican, Inc. | Apparatus and methods for concentrating platelet-rich plasma |
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EP2702388A4 (en) | 2015-05-13 |
KR102070829B1 (ko) | 2020-01-29 |
WO2012149314A1 (en) | 2012-11-01 |
EP2702388B1 (en) | 2021-08-04 |
EP2702388A1 (en) | 2014-03-05 |
CA2834398C (en) | 2020-06-23 |
CN103649715B (zh) | 2018-01-16 |
CA3077972C (en) | 2022-04-12 |
KR20140033372A (ko) | 2014-03-18 |
EP3822612A1 (en) | 2021-05-19 |
CA2834398A1 (en) | 2012-11-01 |
US20180304251A1 (en) | 2018-10-25 |
CA3077972A1 (en) | 2012-11-01 |
JP2014513300A (ja) | 2014-05-29 |
KR20210131459A (ko) | 2021-11-02 |
KR20200010578A (ko) | 2020-01-30 |
US10016757B2 (en) | 2018-07-10 |
CN103649715A (zh) | 2014-03-19 |
KR102418162B1 (ko) | 2022-07-06 |
US20130109042A1 (en) | 2013-05-02 |
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