JP6106264B2 - 乳酸塩の定量のための遺伝学的にコードされたプローブならびに代謝速度および乳酸塩輸送の定量方法 - Google Patents

乳酸塩の定量のための遺伝学的にコードされたプローブならびに代謝速度および乳酸塩輸送の定量方法 Download PDF

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JP6106264B2
JP6106264B2 JP2015505696A JP2015505696A JP6106264B2 JP 6106264 B2 JP6106264 B2 JP 6106264B2 JP 2015505696 A JP2015505696 A JP 2015505696A JP 2015505696 A JP2015505696 A JP 2015505696A JP 6106264 B2 JP6106264 B2 JP 6106264B2
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lactate
rate
sequence number
nanosensor
concentration
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オルメド,ルイス フェリペ バロス
オルメド,ルイス フェリペ バロス
マルティン,アレハンドロ サン
マルティン,アレハンドロ サン
チャルペンティエール,セバスティアン セバーリョ
チャルペンティエール,セバスティアン セバーリョ
フロマー,ウルフ,ビー
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CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA
Carnegie Institution of Washington
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CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA
Carnegie Institution of Washington
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    • GPHYSICS
    • G01MEASURING; TESTING
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    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
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    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
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    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • GPHYSICS
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    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5038Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving detection of metabolites per se
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

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JP2015505696A 2012-04-13 2012-04-13 乳酸塩の定量のための遺伝学的にコードされたプローブならびに代謝速度および乳酸塩輸送の定量方法 Expired - Fee Related JP6106264B2 (ja)

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PCT/US2012/033639 WO2013154587A1 (en) 2012-04-13 2012-04-13 Genetically encoded probe for quantification of lactate, and methods for quantifying metabolic rates and lactate transport

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JP2015512935A JP2015512935A (ja) 2015-04-30
JP2015512935A5 JP2015512935A5 (enExample) 2016-05-12
JP6106264B2 true JP6106264B2 (ja) 2017-03-29

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US (1) US10466233B2 (enExample)
EP (1) EP2836503B1 (enExample)
JP (1) JP6106264B2 (enExample)
CA (1) CA2870271C (enExample)
DK (1) DK2836503T3 (enExample)
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WO2015025192A1 (en) * 2013-08-20 2015-02-26 Centro De Estudios Cientificos De Valdivia Genetically encoded probe for quantification of pyruvate concentration and methods of using the same
DE102015115640B4 (de) * 2015-09-16 2017-06-29 Stiftung Caesar Center Of Advanced European Studies And Research Verfahren zum Nachweis von Liganden vermittels Biosensoren
EP3377897B1 (en) * 2015-11-20 2024-05-01 Duke University Lactate biosensors and uses thereof
EP3377904B1 (en) 2015-11-20 2024-05-01 Duke University Urea biosensors and uses thereof
US20180371643A1 (en) * 2015-11-30 2018-12-27 Council Of Scientific & Industrial Research Method of Identification of Combinatorial Enzymatic Reaction Targets in Glioblastoma Specific Metabolic Network
WO2018152480A1 (en) * 2017-02-20 2018-08-23 Richard Postrel Method for precise identification, targeting and delivery of directed therapies for destruction of cancerous cells
US11175281B2 (en) 2018-08-21 2021-11-16 International Business Machines Corporation Well plate cover with embedded electronic sensors for monitoring cell metabolism
KR102167605B1 (ko) * 2019-05-20 2020-10-19 울산과학기술원 슈도모나스 플루오레센스 유래 신규 D-LldR 전사조절인자 기반의 D(-)-젖산 검출용 바이오 센서
WO2021033015A1 (en) * 2019-08-22 2021-02-25 Centro De Estudios Científicos De Valdivia Genetically encoded probe for detection and quantification of lactate/pyruvate ratio with high spatial and temporal resolution
CN113004420B (zh) * 2019-12-19 2023-02-24 华东理工大学 乳酸光学探针及其制备方法和应用

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JP3829252B2 (ja) * 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
CA2457059A1 (en) 2001-08-10 2003-02-20 Ahram Biosystems Inc. System for detecting protease
WO2003025220A2 (en) * 2001-09-18 2003-03-27 Carnegie Institution Of Washington Fusion proteins useful for detecting analytes
AU2005295656B2 (en) * 2004-10-14 2011-08-04 Carnegie Institution Of Washington Development of sensitive FRET sensors and methods of using the same
CA2599852A1 (en) * 2005-03-04 2006-09-14 Carnegie Institution Of Washington Environmentally stable sensors and methods of using the same
US7790868B2 (en) * 2005-05-20 2010-09-07 The Governors Of The University Of Alberta Teal fluorescent proteins (mTFP1)
US8173863B2 (en) * 2005-10-14 2012-05-08 Carnegie Institution Of Washington Sucrose biosensors and methods of using the same

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JP2015512935A (ja) 2015-04-30
EP2836503A1 (en) 2015-02-18
WO2013154587A1 (en) 2013-10-17
EP2836503B1 (en) 2016-06-08
EP2836503A4 (en) 2015-03-25
DK2836503T3 (en) 2016-09-19
CA2870271C (en) 2020-06-30
ES2602321T3 (es) 2017-02-20
US20150072375A1 (en) 2015-03-12
US10466233B2 (en) 2019-11-05
CA2870271A1 (en) 2013-10-17

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