DK2836503T3 - Genetically coded probe for quantifying lactate, and methods for quantifying metabolic rates and lactate transport. - Google Patents

Genetically coded probe for quantifying lactate, and methods for quantifying metabolic rates and lactate transport. Download PDF

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Publication number
DK2836503T3
DK2836503T3 DK12874169.1T DK12874169T DK2836503T3 DK 2836503 T3 DK2836503 T3 DK 2836503T3 DK 12874169 T DK12874169 T DK 12874169T DK 2836503 T3 DK2836503 T3 DK 2836503T3
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DK12874169.1T
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Olmedo Luis Felipe Barros
Martin Alejandro San
Charpentier Sebastian Ceballo
Wolf B Frommer
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Centro De Estudios Cientificos De Valdivia
Carnegie Inst Of Washington
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5038—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving detection of metabolites per se
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DK12874169.1T 2012-04-13 2012-04-13 Genetically coded probe for quantifying lactate, and methods for quantifying metabolic rates and lactate transport. DK2836503T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2012/033639 WO2013154587A1 (en) 2012-04-13 2012-04-13 Genetically encoded probe for quantification of lactate, and methods for quantifying metabolic rates and lactate transport

Publications (1)

Publication Number Publication Date
DK2836503T3 true DK2836503T3 (en) 2016-09-19

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DK12874169.1T DK2836503T3 (en) 2012-04-13 2012-04-13 Genetically coded probe for quantifying lactate, and methods for quantifying metabolic rates and lactate transport.

Country Status (7)

Country Link
US (1) US10466233B2 (enExample)
EP (1) EP2836503B1 (enExample)
JP (1) JP6106264B2 (enExample)
CA (1) CA2870271C (enExample)
DK (1) DK2836503T3 (enExample)
ES (1) ES2602321T3 (enExample)
WO (1) WO2013154587A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015025192A1 (en) * 2013-08-20 2015-02-26 Centro De Estudios Cientificos De Valdivia Genetically encoded probe for quantification of pyruvate concentration and methods of using the same
DE102015115640B4 (de) * 2015-09-16 2017-06-29 Stiftung Caesar Center Of Advanced European Studies And Research Verfahren zum Nachweis von Liganden vermittels Biosensoren
EP3377897B1 (en) * 2015-11-20 2024-05-01 Duke University Lactate biosensors and uses thereof
EP3377904B1 (en) 2015-11-20 2024-05-01 Duke University Urea biosensors and uses thereof
US20180371643A1 (en) * 2015-11-30 2018-12-27 Council Of Scientific & Industrial Research Method of Identification of Combinatorial Enzymatic Reaction Targets in Glioblastoma Specific Metabolic Network
WO2018152480A1 (en) * 2017-02-20 2018-08-23 Richard Postrel Method for precise identification, targeting and delivery of directed therapies for destruction of cancerous cells
US11175281B2 (en) 2018-08-21 2021-11-16 International Business Machines Corporation Well plate cover with embedded electronic sensors for monitoring cell metabolism
KR102167605B1 (ko) * 2019-05-20 2020-10-19 울산과학기술원 슈도모나스 플루오레센스 유래 신규 D-LldR 전사조절인자 기반의 D(-)-젖산 검출용 바이오 센서
WO2021033015A1 (en) * 2019-08-22 2021-02-25 Centro De Estudios Científicos De Valdivia Genetically encoded probe for detection and quantification of lactate/pyruvate ratio with high spatial and temporal resolution
CN113004420B (zh) * 2019-12-19 2023-02-24 华东理工大学 乳酸光学探针及其制备方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3829252B2 (ja) * 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
CA2457059A1 (en) 2001-08-10 2003-02-20 Ahram Biosystems Inc. System for detecting protease
WO2003025220A2 (en) * 2001-09-18 2003-03-27 Carnegie Institution Of Washington Fusion proteins useful for detecting analytes
AU2005295656B2 (en) * 2004-10-14 2011-08-04 Carnegie Institution Of Washington Development of sensitive FRET sensors and methods of using the same
CA2599852A1 (en) * 2005-03-04 2006-09-14 Carnegie Institution Of Washington Environmentally stable sensors and methods of using the same
US7790868B2 (en) * 2005-05-20 2010-09-07 The Governors Of The University Of Alberta Teal fluorescent proteins (mTFP1)
US8173863B2 (en) * 2005-10-14 2012-05-08 Carnegie Institution Of Washington Sucrose biosensors and methods of using the same

Also Published As

Publication number Publication date
JP2015512935A (ja) 2015-04-30
EP2836503A1 (en) 2015-02-18
WO2013154587A1 (en) 2013-10-17
EP2836503B1 (en) 2016-06-08
EP2836503A4 (en) 2015-03-25
CA2870271C (en) 2020-06-30
JP6106264B2 (ja) 2017-03-29
ES2602321T3 (es) 2017-02-20
US20150072375A1 (en) 2015-03-12
US10466233B2 (en) 2019-11-05
CA2870271A1 (en) 2013-10-17

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