ES2602321T3 - Sensor codificado genéticamente para la cuantificación de lactato, y métodos para cuantificar tasas metabólicas y transporte de lactato - Google Patents
Sensor codificado genéticamente para la cuantificación de lactato, y métodos para cuantificar tasas metabólicas y transporte de lactato Download PDFInfo
- Publication number
- ES2602321T3 ES2602321T3 ES12874169.1T ES12874169T ES2602321T3 ES 2602321 T3 ES2602321 T3 ES 2602321T3 ES 12874169 T ES12874169 T ES 12874169T ES 2602321 T3 ES2602321 T3 ES 2602321T3
- Authority
- ES
- Spain
- Prior art keywords
- lactate
- pyruvate
- seq
- variant
- corresponds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 title abstract description 58
- 238000000034 method Methods 0.000 title abstract description 11
- 102000000562 Monocarboxylic Acid Transporters Human genes 0.000 title description 3
- 108010041817 Monocarboxylic Acid Transporters Proteins 0.000 title description 3
- 230000037323 metabolic rate Effects 0.000 title description 2
- 238000011002 quantification Methods 0.000 title 1
- 238000001327 Förster resonance energy transfer Methods 0.000 abstract 3
- 238000004113 cell culture Methods 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 102000040945 Transcription factor Human genes 0.000 abstract 1
- 108091023040 Transcription factor Proteins 0.000 abstract 1
- 230000001464 adherent effect Effects 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 102000034287 fluorescent proteins Human genes 0.000 abstract 1
- 108091006047 fluorescent proteins Proteins 0.000 abstract 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 22
- 101710137760 Malonyl-CoA-acyl carrier protein transacylase, mitochondrial Proteins 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 13
- 230000003834 intracellular effect Effects 0.000 description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 239000008103 glucose Substances 0.000 description 8
- 230000005764 inhibitory process Effects 0.000 description 8
- 230000002438 mitochondrial effect Effects 0.000 description 8
- 238000009825 accumulation Methods 0.000 description 7
- 210000001130 astrocyte Anatomy 0.000 description 7
- 108010078791 Carrier Proteins Proteins 0.000 description 4
- 230000001086 cytosolic effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000002414 glycolytic effect Effects 0.000 description 3
- 210000003470 mitochondria Anatomy 0.000 description 3
- 230000006677 mitochondrial metabolism Effects 0.000 description 3
- 230000001154 acute effect Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 150000001540 azides Chemical class 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000034659 glycolysis Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000002407 ATP formation Effects 0.000 description 1
- 108050001175 Connexin Proteins 0.000 description 1
- 102000010970 Connexin Human genes 0.000 description 1
- 102000018711 Facilitative Glucose Transport Proteins Human genes 0.000 description 1
- 108091052347 Glucose transporter family Proteins 0.000 description 1
- 108010026389 Gramicidin Proteins 0.000 description 1
- 102000005548 Hexokinase Human genes 0.000 description 1
- 108700040460 Hexokinases Proteins 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- GBOGMAARMMDZGR-UHFFFAOYSA-N UNPD149280 Natural products N1C(=O)C23OC(=O)C=CC(O)CCCC(C)CC=CC3C(O)C(=C)C(C)C2C1CC1=CC=CC=C1 GBOGMAARMMDZGR-UHFFFAOYSA-N 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000006567 cellular energy metabolism Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- GBOGMAARMMDZGR-JREHFAHYSA-N cytochalasin B Natural products C[C@H]1CCC[C@@H](O)C=CC(=O)O[C@@]23[C@H](C=CC1)[C@H](O)C(=C)[C@@H](C)[C@@H]2[C@H](Cc4ccccc4)NC3=O GBOGMAARMMDZGR-JREHFAHYSA-N 0.000 description 1
- GBOGMAARMMDZGR-TYHYBEHESA-N cytochalasin B Chemical compound C([C@H]1[C@@H]2[C@@H](C([C@@H](O)[C@@H]3/C=C/C[C@H](C)CCC[C@@H](O)/C=C/C(=O)O[C@@]23C(=O)N1)=C)C)C1=CC=CC=C1 GBOGMAARMMDZGR-TYHYBEHESA-N 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000012912 drug discovery process Methods 0.000 description 1
- DANUORFCFTYTSZ-UHFFFAOYSA-N epinigericin Natural products O1C2(C(CC(C)(O2)C2OC(C)(CC2)C2C(CC(O2)C2C(CC(C)C(O)(CO)O2)C)C)C)C(C)C(OC)CC1CC1CCC(C)C(C(C)C(O)=O)O1 DANUORFCFTYTSZ-UHFFFAOYSA-N 0.000 description 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 1
- 229960004905 gramicidin Drugs 0.000 description 1
- ZWCXYZRRTRDGQE-SORVKSEFSA-N gramicidina Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@H](NC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](NC=O)C(C)C)CC(C)C)C(=O)NCCO)=CNC2=C1 ZWCXYZRRTRDGQE-SORVKSEFSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004941 influx Effects 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 210000000350 mc(t) Anatomy 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229940126127 monocarboxylate transporter inhibitor Drugs 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- DANUORFCFTYTSZ-BIBFWWMMSA-N nigericin Chemical compound C([C@@H]1C[C@H]([C@H]([C@]2([C@@H](C[C@](C)(O2)C2O[C@@](C)(CC2)C2[C@H](CC(O2)[C@@H]2[C@H](C[C@@H](C)[C@](O)(CO)O2)C)C)C)O1)C)OC)[C@H]1CC[C@H](C)C([C@@H](C)C(O)=O)O1 DANUORFCFTYTSZ-BIBFWWMMSA-N 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000010627 oxidative phosphorylation Effects 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- AFVLVVWMAFSXCK-UHFFFAOYSA-N α-cyano-4-hydroxycinnamic acid Chemical compound OC(=O)C(C#N)=CC1=CC=C(O)C=C1 AFVLVVWMAFSXCK-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5038—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving detection of metabolites per se
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US2012/033639 WO2013154587A1 (en) | 2012-04-13 | 2012-04-13 | Genetically encoded probe for quantification of lactate, and methods for quantifying metabolic rates and lactate transport |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2602321T3 true ES2602321T3 (es) | 2017-02-20 |
Family
ID=49327999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12874169.1T Active ES2602321T3 (es) | 2012-04-13 | 2012-04-13 | Sensor codificado genéticamente para la cuantificación de lactato, y métodos para cuantificar tasas metabólicas y transporte de lactato |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10466233B2 (enExample) |
| EP (1) | EP2836503B1 (enExample) |
| JP (1) | JP6106264B2 (enExample) |
| CA (1) | CA2870271C (enExample) |
| DK (1) | DK2836503T3 (enExample) |
| ES (1) | ES2602321T3 (enExample) |
| WO (1) | WO2013154587A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015025192A1 (en) * | 2013-08-20 | 2015-02-26 | Centro De Estudios Cientificos De Valdivia | Genetically encoded probe for quantification of pyruvate concentration and methods of using the same |
| DE102015115640B4 (de) * | 2015-09-16 | 2017-06-29 | Stiftung Caesar Center Of Advanced European Studies And Research | Verfahren zum Nachweis von Liganden vermittels Biosensoren |
| EP3377897B1 (en) * | 2015-11-20 | 2024-05-01 | Duke University | Lactate biosensors and uses thereof |
| EP3377904B1 (en) | 2015-11-20 | 2024-05-01 | Duke University | Urea biosensors and uses thereof |
| US20180371643A1 (en) * | 2015-11-30 | 2018-12-27 | Council Of Scientific & Industrial Research | Method of Identification of Combinatorial Enzymatic Reaction Targets in Glioblastoma Specific Metabolic Network |
| WO2018152480A1 (en) * | 2017-02-20 | 2018-08-23 | Richard Postrel | Method for precise identification, targeting and delivery of directed therapies for destruction of cancerous cells |
| US11175281B2 (en) | 2018-08-21 | 2021-11-16 | International Business Machines Corporation | Well plate cover with embedded electronic sensors for monitoring cell metabolism |
| KR102167605B1 (ko) * | 2019-05-20 | 2020-10-19 | 울산과학기술원 | 슈도모나스 플루오레센스 유래 신규 D-LldR 전사조절인자 기반의 D(-)-젖산 검출용 바이오 센서 |
| WO2021033015A1 (en) * | 2019-08-22 | 2021-02-25 | Centro De Estudios Científicos De Valdivia | Genetically encoded probe for detection and quantification of lactate/pyruvate ratio with high spatial and temporal resolution |
| CN113004420B (zh) * | 2019-12-19 | 2023-02-24 | 华东理工大学 | 乳酸光学探针及其制备方法和应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3829252B2 (ja) * | 2001-06-08 | 2006-10-04 | 独立行政法人理化学研究所 | 蛍光蛋白質 |
| CA2457059A1 (en) | 2001-08-10 | 2003-02-20 | Ahram Biosystems Inc. | System for detecting protease |
| WO2003025220A2 (en) * | 2001-09-18 | 2003-03-27 | Carnegie Institution Of Washington | Fusion proteins useful for detecting analytes |
| AU2005295656B2 (en) * | 2004-10-14 | 2011-08-04 | Carnegie Institution Of Washington | Development of sensitive FRET sensors and methods of using the same |
| CA2599852A1 (en) * | 2005-03-04 | 2006-09-14 | Carnegie Institution Of Washington | Environmentally stable sensors and methods of using the same |
| US7790868B2 (en) * | 2005-05-20 | 2010-09-07 | The Governors Of The University Of Alberta | Teal fluorescent proteins (mTFP1) |
| US8173863B2 (en) * | 2005-10-14 | 2012-05-08 | Carnegie Institution Of Washington | Sucrose biosensors and methods of using the same |
-
2012
- 2012-04-13 CA CA2870271A patent/CA2870271C/en not_active Expired - Fee Related
- 2012-04-13 JP JP2015505696A patent/JP6106264B2/ja not_active Expired - Fee Related
- 2012-04-13 DK DK12874169.1T patent/DK2836503T3/en active
- 2012-04-13 US US14/394,018 patent/US10466233B2/en not_active Expired - Fee Related
- 2012-04-13 EP EP12874169.1A patent/EP2836503B1/en not_active Not-in-force
- 2012-04-13 WO PCT/US2012/033639 patent/WO2013154587A1/en not_active Ceased
- 2012-04-13 ES ES12874169.1T patent/ES2602321T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015512935A (ja) | 2015-04-30 |
| EP2836503A1 (en) | 2015-02-18 |
| WO2013154587A1 (en) | 2013-10-17 |
| EP2836503B1 (en) | 2016-06-08 |
| EP2836503A4 (en) | 2015-03-25 |
| DK2836503T3 (en) | 2016-09-19 |
| CA2870271C (en) | 2020-06-30 |
| JP6106264B2 (ja) | 2017-03-29 |
| US20150072375A1 (en) | 2015-03-12 |
| US10466233B2 (en) | 2019-11-05 |
| CA2870271A1 (en) | 2013-10-17 |
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