JP2015512935A5 - - Google Patents
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- Publication number
- JP2015512935A5 JP2015512935A5 JP2015505696A JP2015505696A JP2015512935A5 JP 2015512935 A5 JP2015512935 A5 JP 2015512935A5 JP 2015505696 A JP2015505696 A JP 2015505696A JP 2015505696 A JP2015505696 A JP 2015505696A JP 2015512935 A5 JP2015512935 A5 JP 2015512935A5
- Authority
- JP
- Japan
- Prior art keywords
- lactate
- sequence number
- rate
- nanosensor
- fret
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims 67
- 210000004027 cell Anatomy 0.000 claims 24
- 238000001327 Förster resonance energy transfer Methods 0.000 claims 14
- 210000001519 tissue Anatomy 0.000 claims 11
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims 10
- 238000004113 cell culture Methods 0.000 claims 10
- 238000000034 method Methods 0.000 claims 10
- 108091006047 fluorescent proteins Proteins 0.000 claims 6
- 102000034287 fluorescent proteins Human genes 0.000 claims 6
- 230000002438 mitochondrial effect Effects 0.000 claims 6
- 238000009825 accumulation Methods 0.000 claims 5
- 239000000725 suspension Substances 0.000 claims 5
- 102000000562 Monocarboxylic Acid Transporters Human genes 0.000 claims 4
- 108010041817 Monocarboxylic Acid Transporters Proteins 0.000 claims 4
- 238000000338 in vitro Methods 0.000 claims 4
- 230000002452 interceptive effect Effects 0.000 claims 4
- 230000003834 intracellular effect Effects 0.000 claims 4
- 210000003470 mitochondria Anatomy 0.000 claims 3
- 229940126127 monocarboxylate transporter inhibitor Drugs 0.000 claims 3
- 108091005957 yellow fluorescent proteins Proteins 0.000 claims 3
- YMHOBZXQZVXHBM-UHFFFAOYSA-N 2,5-dimethoxy-4-bromophenethylamine Chemical compound COC1=CC(CCN)=C(OC)C=C1Br YMHOBZXQZVXHBM-UHFFFAOYSA-N 0.000 claims 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims 2
- 241000545067 Venus Species 0.000 claims 2
- 230000001580 bacterial effect Effects 0.000 claims 2
- 108091005948 blue fluorescent proteins Proteins 0.000 claims 2
- 108010082025 cyan fluorescent protein Proteins 0.000 claims 2
- 239000005090 green fluorescent protein Substances 0.000 claims 2
- 241001465754 Metazoa Species 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 230000001464 adherent effect Effects 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 238000001727 in vivo Methods 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 150000003893 lactate salts Chemical class 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
- 239000000178 monomer Substances 0.000 claims 1
- 150000007523 nucleic acids Chemical group 0.000 claims 1
- 230000002103 transcriptional effect Effects 0.000 claims 1
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US2012/033639 WO2013154587A1 (en) | 2012-04-13 | 2012-04-13 | Genetically encoded probe for quantification of lactate, and methods for quantifying metabolic rates and lactate transport |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015512935A JP2015512935A (ja) | 2015-04-30 |
| JP2015512935A5 true JP2015512935A5 (enExample) | 2016-05-12 |
| JP6106264B2 JP6106264B2 (ja) | 2017-03-29 |
Family
ID=49327999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015505696A Expired - Fee Related JP6106264B2 (ja) | 2012-04-13 | 2012-04-13 | 乳酸塩の定量のための遺伝学的にコードされたプローブならびに代謝速度および乳酸塩輸送の定量方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10466233B2 (enExample) |
| EP (1) | EP2836503B1 (enExample) |
| JP (1) | JP6106264B2 (enExample) |
| CA (1) | CA2870271C (enExample) |
| DK (1) | DK2836503T3 (enExample) |
| ES (1) | ES2602321T3 (enExample) |
| WO (1) | WO2013154587A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015025192A1 (en) * | 2013-08-20 | 2015-02-26 | Centro De Estudios Cientificos De Valdivia | Genetically encoded probe for quantification of pyruvate concentration and methods of using the same |
| DE102015115640B4 (de) * | 2015-09-16 | 2017-06-29 | Stiftung Caesar Center Of Advanced European Studies And Research | Verfahren zum Nachweis von Liganden vermittels Biosensoren |
| EP3377897B1 (en) * | 2015-11-20 | 2024-05-01 | Duke University | Lactate biosensors and uses thereof |
| EP3377904B1 (en) | 2015-11-20 | 2024-05-01 | Duke University | Urea biosensors and uses thereof |
| US20180371643A1 (en) * | 2015-11-30 | 2018-12-27 | Council Of Scientific & Industrial Research | Method of Identification of Combinatorial Enzymatic Reaction Targets in Glioblastoma Specific Metabolic Network |
| WO2018152480A1 (en) * | 2017-02-20 | 2018-08-23 | Richard Postrel | Method for precise identification, targeting and delivery of directed therapies for destruction of cancerous cells |
| US11175281B2 (en) | 2018-08-21 | 2021-11-16 | International Business Machines Corporation | Well plate cover with embedded electronic sensors for monitoring cell metabolism |
| KR102167605B1 (ko) * | 2019-05-20 | 2020-10-19 | 울산과학기술원 | 슈도모나스 플루오레센스 유래 신규 D-LldR 전사조절인자 기반의 D(-)-젖산 검출용 바이오 센서 |
| WO2021033015A1 (en) * | 2019-08-22 | 2021-02-25 | Centro De Estudios Científicos De Valdivia | Genetically encoded probe for detection and quantification of lactate/pyruvate ratio with high spatial and temporal resolution |
| CN113004420B (zh) * | 2019-12-19 | 2023-02-24 | 华东理工大学 | 乳酸光学探针及其制备方法和应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3829252B2 (ja) * | 2001-06-08 | 2006-10-04 | 独立行政法人理化学研究所 | 蛍光蛋白質 |
| CA2457059A1 (en) | 2001-08-10 | 2003-02-20 | Ahram Biosystems Inc. | System for detecting protease |
| WO2003025220A2 (en) * | 2001-09-18 | 2003-03-27 | Carnegie Institution Of Washington | Fusion proteins useful for detecting analytes |
| AU2005295656B2 (en) * | 2004-10-14 | 2011-08-04 | Carnegie Institution Of Washington | Development of sensitive FRET sensors and methods of using the same |
| CA2599852A1 (en) * | 2005-03-04 | 2006-09-14 | Carnegie Institution Of Washington | Environmentally stable sensors and methods of using the same |
| US7790868B2 (en) * | 2005-05-20 | 2010-09-07 | The Governors Of The University Of Alberta | Teal fluorescent proteins (mTFP1) |
| US8173863B2 (en) * | 2005-10-14 | 2012-05-08 | Carnegie Institution Of Washington | Sucrose biosensors and methods of using the same |
-
2012
- 2012-04-13 CA CA2870271A patent/CA2870271C/en not_active Expired - Fee Related
- 2012-04-13 JP JP2015505696A patent/JP6106264B2/ja not_active Expired - Fee Related
- 2012-04-13 DK DK12874169.1T patent/DK2836503T3/en active
- 2012-04-13 US US14/394,018 patent/US10466233B2/en not_active Expired - Fee Related
- 2012-04-13 EP EP12874169.1A patent/EP2836503B1/en not_active Not-in-force
- 2012-04-13 WO PCT/US2012/033639 patent/WO2013154587A1/en not_active Ceased
- 2012-04-13 ES ES12874169.1T patent/ES2602321T3/es active Active
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