JP5987895B2 - 核酸増幅反応用マイクロチップの製造方法 - Google Patents

核酸増幅反応用マイクロチップの製造方法 Download PDF

Info

Publication number
JP5987895B2
JP5987895B2 JP2014503511A JP2014503511A JP5987895B2 JP 5987895 B2 JP5987895 B2 JP 5987895B2 JP 2014503511 A JP2014503511 A JP 2014503511A JP 2014503511 A JP2014503511 A JP 2014503511A JP 5987895 B2 JP5987895 B2 JP 5987895B2
Authority
JP
Japan
Prior art keywords
nucleic acid
microchip
acid amplification
reagent
reagent solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2014503511A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2013132891A1 (ja
Inventor
真寛 松本
真寛 松本
佐藤　正樹
正樹 佐藤
英俊 渡辺
英俊 渡辺
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sony Corp
Original Assignee
Sony Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sony Corp filed Critical Sony Corp
Priority to JP2014503511A priority Critical patent/JP5987895B2/ja
Publication of JPWO2013132891A1 publication Critical patent/JPWO2013132891A1/ja
Application granted granted Critical
Publication of JP5987895B2 publication Critical patent/JP5987895B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
JP2014503511A 2012-03-08 2013-01-16 核酸増幅反応用マイクロチップの製造方法 Active JP5987895B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2014503511A JP5987895B2 (ja) 2012-03-08 2013-01-16 核酸増幅反応用マイクロチップの製造方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2012052322 2012-03-08
JP2012052322 2012-03-08
PCT/JP2013/050652 WO2013132891A1 (ja) 2012-03-08 2013-01-16 核酸増幅反応用マイクロチップの製造方法
JP2014503511A JP5987895B2 (ja) 2012-03-08 2013-01-16 核酸増幅反応用マイクロチップの製造方法

Publications (2)

Publication Number Publication Date
JPWO2013132891A1 JPWO2013132891A1 (ja) 2015-07-30
JP5987895B2 true JP5987895B2 (ja) 2016-09-07

Family

ID=49116378

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014503511A Active JP5987895B2 (ja) 2012-03-08 2013-01-16 核酸増幅反応用マイクロチップの製造方法

Country Status (8)

Country Link
US (1) US9545630B2 (Direct)
EP (1) EP2824172B1 (Direct)
JP (1) JP5987895B2 (Direct)
KR (1) KR20140143139A (Direct)
CN (1) CN104160011A (Direct)
IN (1) IN2014MN01628A (Direct)
RU (1) RU2014135538A (Direct)
WO (1) WO2013132891A1 (Direct)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3430410B1 (en) 2016-03-15 2022-04-27 Abbott Molecular Inc. Method for automated analysis
ES2891349T3 (es) * 2016-12-13 2022-01-27 Eiken Chemical Microchip
CN110885902B (zh) * 2018-09-10 2022-10-21 北京亿森宝生物科技有限公司 用于检测猪蓝耳病毒并鉴别从中猪蓝耳病毒高致病经典变异株的冻干微芯片、试剂盒及方法
CN110885899B (zh) * 2018-09-10 2023-04-14 中国动物疫病预防控制中心（农业部屠宰技术中心） 用于鉴别16种禽病病原冻干微芯片、试剂盒及方法
WO2025094815A1 (ja) * 2023-10-30 2025-05-08 Ｔｏｐｐａｎホールディングス株式会社 流体デバイス

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0775518B2 (ja) * 1989-09-29 1995-08-16 勝 大鶴 ▲高▼血圧、▲高▼脂血症、肥満の予防及び治療に有効な飲食用キノコタンパク質及びその抽出方法
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
JPH08291078A (ja) * 1995-04-21 1996-11-05 M I O:Kk 高血圧及び高脂血症の予防・治療に有効で且つ抗腫瘍作用を有する飲食用キノコタンパク質、及び肥満の予防・治療に有効で且つ抗腫瘍作用を有する飲食用キノコタンパク質、並びにそれらの抽出方法
JPH10234822A (ja) * 1997-02-28 1998-09-08 Material Eng Tech Lab Inc 凍結乾燥用容器
US6635295B1 (en) 1999-05-19 2003-10-21 National Agricultural Research Organization Method for freeze-drying and freeze-dried product
US6875619B2 (en) * 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
ES2180416B1 (es) 2001-03-12 2004-06-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen.
GB0414815D0 (en) * 2004-07-02 2004-08-04 Secr Defence Method for stabilising reagents which are useful for nucleic acid amplification
CN100460013C (zh) * 2006-09-05 2009-02-11 重庆康卫生物科技有限公司 口服重组幽门螺杆菌疫苗及其制备方法
JP5685538B2 (ja) * 2008-09-24 2015-03-18 ストラウス ホールディングス インコーポレイテッド 分析物を検出するためのキットおよび装置
EP2784508B1 (en) * 2008-12-25 2017-06-14 Universal Bio Research Co., Ltd. Automated nucleic acid assay system
WO2010088496A1 (en) * 2009-01-30 2010-08-05 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
JP5691187B2 (ja) * 2010-02-10 2015-04-01 ソニー株式会社 核酸増幅反応用マイクロチップ及びその製造方法
JP5786295B2 (ja) * 2010-06-22 2015-09-30 ソニー株式会社 核酸等温増幅反応用マイクロチップ及びその製造方法並びに核酸等温増幅方法
JP2012080870A (ja) * 2010-09-16 2012-04-26 Sony Corp 核酸定量方法及び核酸増幅反応用マイクロチップ

Also Published As

Publication number Publication date
CN104160011A (zh) 2014-11-19
EP2824172A1 (en) 2015-01-14
JPWO2013132891A1 (ja) 2015-07-30
EP2824172A4 (en) 2015-11-04
IN2014MN01628A (Direct) 2015-05-15
RU2014135538A (ru) 2016-03-20
US9545630B2 (en) 2017-01-17
WO2013132891A1 (ja) 2013-09-12
KR20140143139A (ko) 2014-12-15
EP2824172B1 (en) 2017-09-06
US20150017318A1 (en) 2015-01-15

Similar Documents

Publication Publication Date Title
US8961899B2 (en) Integrated sample preparation systems and stabilized enzyme mixtures
CA3018687C (en) Multi-primer amplification method for barcoding of target nucleic acids
TWI539001B (zh) 快速多重擴增目標核酸的方法
JP5987895B2 (ja) 核酸増幅反応用マイクロチップの製造方法
CN102741408B (zh) 用于核酸扩增反应的微芯片及其制造方法
US20230405586A1 (en) System and self-metering cartridges for point of care bioassays
EP3073273A1 (en) Micro chamber plate
WO2007149903A2 (en) Multi-stage amplification reactions by control of sequence replication times
JP2014011974A (ja) 化学反応用マイクロチップ及びその製造方法
US11898197B2 (en) System and self-metering cartridges for point of care bioassays
US20130102062A1 (en) Microchip for nucleic acid amplification reaction and method of producing the same
US20250389715A1 (en) Point-of-care bioassay systems and methods of making and using the same
CN1775796A (zh) 核酸制备
US12428674B2 (en) System and self-metering cartridges for point of care bioassays
KR20230058722A (ko) 핵산 반응 챔버, 이를 이용한 핵산 반응 방법 및 이를 포함하는 샘플 처리용 카트리지
HK1092157A (en) Nucleic acid preparation
JP2015159760A (ja) 核酸増幅チップ

Legal Events

Date Code Title Description
A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20151222

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160217

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160712

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160725

R151 Written notification of patent or utility model registration

Ref document number: 5987895

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R151

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250