JP5974211B2 - A new Ural elephant and its growing stron - Google Patents

Description

  The present invention relates to a novel Uralphanthus and a stron for cultivation thereof. More specifically, the present invention relates to a glycyrrhizic acid high yielding larval elephant that can obtain glycyrrhizic acid in a high yield.

  Licorice belongs to the leguminous family and is a perennial plant that grows naturally in dry regions such as southern Russia, Mongolia, northern China to western China, and Europe. Some dried licorice roots and strons (underground stems) are called licorice, and have been widely used in traditional Chinese medicine as antispasmodics, analgesics, antitussives, and expectorants.

  In particular, in the 15th revision of the Japanese Pharmacopoeia, as the basic plant of licorice, Uralcanzo (trade name: Tohoku licorice, English name: licorice, scientific name: Glycyrrhiza uralensis Fischer) and Spanish licorice (product) Name: Nishikita licorice, English name: licorice, scientific name: Glycyrrhiza glabra Linne). The Japanese Pharmacopoeia stipulates that licorice contains 2.5% or more of glycyrrhizic acid, which is a triterpenoid saponin, which is a main component.

  Glycyrrhizic acid has a liver function enhancing action, an antitumor action, an anti-inflammatory action, an antiviral action, an antiallergic action, an antiatopic action, and the like, and is extremely useful as a pharmaceutical product. Glycyrrhizic acid has a unique sweetness of about 150 times that of sucrose, and is therefore consumed in large quantities as a sweetener such as soy sauce and confectionery, as well as pharmaceuticals.

  Currently, almost all licorice in the Japanese market is covered with wild products, and the current situation is that it relies on imports from the country of origin such as China. The depletion of resources is a problem. For this reason, various researches are tried about field cultivation of licorice (for example, refer to patent documents 1).

  However, because cultivated licorice has a difference in the content of glycyrrhizic acid due to the influence of the cultivation area, weather, etc., it is difficult to produce glycyrrhizic acid stably and in large quantities. There is a problem that it is extremely rare that it becomes 2.5% or more of the reference value defined in (1), and the cause of these problems cannot be elucidated.

JP-A-6-205618

  The present invention has been made in view of the above-described problems of the prior art, and an object thereof is to provide a glycyrrhizic acid high-yield uracanzo that can obtain glycyrrhizic acid in a high yield. .

  The above object of the present invention is achieved by the following means.

(1) That is, the present invention relates to Uralphanthus No. 1 preserved at the Hokkaido Research Department, Medicinal Plant Resource Research Center , National Institute of Biomedical Innovation , Health and Nutrition. 5, no. 20, no. 46, no. 53, no. 70, no. 75 or No. 79. A glycyrrhizic uralensis fisher having a high yield of glycyrrhizic acid, wherein the underground yield of glycyrrhizic acid is 3.5 to 15.0 g.

(2) The present invention also relates to Uralphanthus No. 1 preserved in the Hokkaido Research Department, Research Center for Medicinal Plant Resources , National Institute of Biomedical Innovation , Health and Nutrition. 10 or No. 15. A glycyrrhizic uralensis fisher having a high yield of glycyrrhizic acid, wherein the yield of glycyrrhizic acid is 3.5 to 15.0 g.

(3) The present invention is also the glycyrrhiza urarensis fisher according to (1) or (2) , wherein the yield of the underground part is 8.5 to 15.0 g.

(4) The present invention is also characterized in that the underground portion content of glycyrrhizic acid is 3.2 to 4.7% and the underground portion raw weight is 150 to 900 g, (1) to (3 ) Is a glycyrrhiz uralensis fisher described in any one of the above.

(5) The glycyrrhizal uralensis fisher according to (1) , wherein the underground content of glycyrrhizic acid is 4.0 to 4.7%, and the raw weight of the underground is 150 to 400 g. It is.

(6) The present invention also relates to underground content of glycyrrhizic acid is 3.2 to 3.7%, and below-ground fresh weight is 800～900G, Gurichiruriza-uralensis Fisher described in (2) It is.

(7) The cultivation of glycyrrhizic acid high yielding glycyrrhiz uralensis fisher characterized in that the present invention is also used for cultivation of glycyrrhizia uralensis fisher according to any one of (1) to (6) For Stron.

  According to the Uralanthus of the present invention, the underground yield of glycyrrhizic acid is 3.5 to 15.0 g and has a very high yield of glycyrrhizic acid, so that it is an antispasmodic, analgesic, antitussive, expectorant, etc. It is extremely useful as a raw material for Chinese herbal medicines, sweeteners such as soy sauce and sweets.

  Moreover, according to the cultivation stron of the present invention, it is possible to easily and efficiently cultivate glycyrrhizic acid high-yielding uralcao. Moreover, it is excellent in preservability, transportability, etc., and is also excellent in handling in the market.

It is the conceptual diagram which showed typically the growth state in the soil of a common Uralphanthus. It is the figure which showed the soil physicochemical property in the field of the Uralphanthus of the present invention. It is the figure which showed various weather conditions in Hokkaido Nayoro-shi during the cultivation period of the Uralphanthus of the present invention. It is the figure which showed various weather conditions in Hokkaido Nayoro-shi during the cultivation period of the Uralphanthus of the present invention. It is the figure which showed various weather conditions in Hokkaido Nayoro-shi during the cultivation period of the Uralphanthus of the present invention. It is the figure which showed the growth character at the time of a 5th-year life, and a glycyrrhizic acid containing characteristic in each strain | stump | stock of the Uralphanthus of this invention. It is the figure which showed the growth character at the time of a 5th-year life, and a glycyrrhizic acid containing characteristic in each strain | stump | stock of the Uralphanthus of this invention. It is the figure which showed the growth character at the time of a 5th-year life, and a glycyrrhizic acid containing characteristic in each strain | stump | stock of the Uralphanthus of this invention. It is the figure which showed the relationship between the color and the glycyrrhizic acid containing characteristic of the root epidermis and cross section in the Uralphanthus of this invention.

  Hereinafter, embodiments of the present invention will be described in detail.

  The present invention relates to a glycyrrhizal high yielding glycerine having an underground yield of glycyrrhizic acid of 3.5 to 15.0 g.

  Ural licorice (trade name: Tohoku licorice, English name: licorice, scientific name: Glycyrrhiza uralensis Fischer) of the present invention is a basic plant of licorice (herbal medicine), generally shown in FIG. It is known that a large amount of glycyrrhizic acid is contained in the subterranean part including the roots and strons of Uralcanus as shown.

  The underground part yield of glycyrrhizic acid in the Ural licorice of the present invention is 3.5 to 15.0 g, preferably 8.5 to 15.0 g per individual. The underground yield of glycyrrhizic acid is the yield of glycyrrhizic acid contained in the underground part of the individual. Specifically, the weight of dry matter in the subterranean larva extracted from the field and the fifteenth It is obtained by multiplication with the content of glycyrrhizic acid calculated by the method prescribed in the revised Japanese Pharmacopoeia.

  Since the yield of glycyrrhizic acid is within the above-mentioned range, the subterranean content of glycyrrhizic acid of the present invention is 3.2 to 4.7% and the underground weight is 150 to 900 g. It is preferable that Specifically, as the larval elephant of the present invention satisfying the relationship between the underground yield of glycyrrhizic acid and the fresh weight of the underground, the underground content of glycyrrhizic acid is 4.0 to 4.7%. In addition, the glycyrrhizic acid high content type of Ural licorice having an underground raw weight of 150 to 400 g, the underground content of glycyrrhizic acid is 3.2 to 3.7%, and the underground raw weight is 800 to 900 g. There is a certain heavy underground larva of the subterranean area. As an example of the former, Ural licorice No. 2 preserved in the Hokkaido Research Department, Research Institute for Medicinal Plant Resources, National Institute of Biomedical Innovation. 5, no. 20, no. 46, no. 53, no. 70, no. 75, no. 79 and the like, and examples of the latter include Uralphanthus No. 10, no. 15 etc. are mentioned.

  In the Japanese Pharmacopoeia, it is stipulated that the underground content of glycyrrhizic acid in licorice is 2.5% or more with respect to the dried product of larval elephant, but the larval elephant of the present invention is as described above. Both satisfy these standards.

  The Uralphanthus of the present invention can be cultivated by a predetermined method. That is, common Uralphanthus can be cultivated in seeds, but cannot increase the yield of glycyrrhizic acid because of its slow growth. Therefore, the cultivation speed can be increased and the yield can be improved by utilizing the nutrient cultivation using the stront of Uralanthus. Specifically, according to the method described in “Medical Plant Cultivation and Quality Evaluation Part 10, p. 51-62, Yakuji Nipposha”, the cultivation stron of the present invention is cut to a length of 15 to 20 cm, and used as the basic fertilizer. Cultivation strons cut to a predetermined length from mid-April to late (from early to mid-May in cold regions) in soil with good drainage and deeply cultivated soil applied with compost, chemical fertilizer and calcium carbonate Transplant. After the second year, it can be cultivated by adding fertilizer with chemical fertilizer and calcium carbonate in mid-April to late April (in cold regions from early to mid-May) and weeding about once a month.

  In addition, the stron for cultivation of the present invention is obtained from the adult larval elephant of the present invention, for example, by the method described in Japanese Patent Application No. 2005-137291, the axillary bud tissue of the present invention is liquid in the dark. It can also be obtained by culturing.

  In addition, since the deposit of the cultivation stron of the present invention to the Patent Organism Depositary of the National Institute of Advanced Industrial Science and Technology has been refused for technical reasons, it is guaranteed that the applicant will sell the cultivation stron.

  Next, the Uralphanthus of the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples, and various modifications can be made without departing from the scope of the present invention. Of course.

  [Example 1]

On June 7, 2004, Hokkaido Research Dept., Research Center for Medicinal Plant Resources, National Institute of Biomedical Research (Location: Nayoro City, Hokkaido (North latitude: 44 ° 21 ', East longitude: 142 ° 27'), Annual average temperature: 5 At 4 ° C. (−30.1 to 30.9 ° C.) and annual precipitation: 860 mm (2003)), Ural licorice seeds (obtained from Inner Mongolia, China, Tochimoto Tenkaido Co., Ltd., introduction number: HK15739- 09) was sown at a cultivation density (12.5 strains / m ² ) having a ridge width of 80 cm and a strain of 10 cm, and cultivated under the fertilization conditions shown below for about 5 years. However, no irrigation or pesticide spraying was performed during the last 5 years. Moreover, weeding was carried out about once a month by using a passenger management machine in the furrow and by hand between the stocks. In addition, the physicochemical property of the soil in the farm field of the said center is as having shown in FIG. 2, and the meteorological conditions during the cultivation of Ural licorice are as having shown in FIGS.
(1) 2004

    1) Basic fertilizer: 100 kg / 10a of calcium carbonate, 2t / 10a of fully matured compost, and 40 kg / 10a of chemical fertilizer 444 on May 26 (N, P, K = 5.6, 5.6, 5.6 kg / 10a) ) Application

2) Additional fertilizer: August 6 chemical fertilizer S325 applied 40kg / 10a (N, P, K = 1.2, 8.8, 6kg / 10a) (2) 2005

1) Additional fertilization: Application of calcium carbonate 100kg / 10a and chemical fertilizer 444 on May 26 at 60kg / 10a (N, P, K = 8.4, 8.4, 8.4kg / 10a), August 4 Application of chemical fertilizer S325 at 60kg / 10a (N, P, K = 1.8, 13.2, 9kg / 10a) (3) 2006

1) Additional fertilizer application: 100 kg / 10a calcium carbonate and 60 kg / 10a (N, P, K = 8.4, 8.4, 8.4 kg / 10a) of chemical fertilizer 444 in late May, chemical fertilizer in early August Application of S325 to 60kg / 10a (N, P, K = 1.8, 13.2, 9kg / 10a) (4) 2007

1) Additional fertilization: Application of calcium carbonate 100kg / 10a and chemical fertilizer 444 90kg / 10a (N, P, K = 12.6, 12.6, 12.6kg / 10a) in late May (5) 2008

    1) Additional fertilizer: None

After that, on August 27, 2008, larvae (approx. 600 individuals) planted in about 40 m × 2 の in the uralcanzo fields (approximately 360 m ² ) were harvested using a degar. Next, on the same day 28, the entangled strons of harvested Uralphanthus were unwound, and 100 individuals with vigorous root growth were selected from these and labeled for each individual (No. 1 to 100), The foliage, roots and strons were cut out and the roots and strons were washed with water.

  [Example 2]

  6 to 8 show the results of observing the color of the root epidermis and the cross section after washing with water for the Uralphanthus (No. 1 to 100) of the present invention obtained in Example 1. FIG. In addition, about the color of the root epidermis, the five-step evaluation which set the thing with strong redness to 5 was performed, and about the color of a cross section, the five-step evaluation which set the thing with strong yellowness to 5 was performed.

  [Example 3]

  About the Ural licorice (No. 1-100) of this invention obtained in Example 1, the weight (raw weight) of the root and the stron after water washing was measured, respectively, and then these raw weights were added together and underground The results of calculating the raw weight of the parts are shown in FIGS.

  As shown in FIGS. 10 and no. The underground weight of 15 Uralph's elephants was 824 g and 850 g. From this, it was confirmed that a larval elephant having very large roots and strons can be obtained as compared with the raw weight of the other subterranean licorice.

  [Example 4]

About the Uralphanthus (No. 1 to 100) of the present invention obtained in Example 1, a portion of about 12 cm from the head of the main root shown in FIG. ) Was collected and dried at 50 ° C. for 10 days using a dryer to obtain a dried product. Next, after the obtained dried product was pulverized with a pulverizer to obtain a powder sample, the powder sample was dried at 50 ° C. for 12 hours using a dryer to obtain a dry powder sample. Next, the obtained dry powder sample was extracted with 50% ethanol, and the content of glycyrrhizic acid (GL content) was measured using HPLC according to the method prescribed in the pharmacopoeia. The results are shown in FIGS. It was shown to.

As shown in FIGS . 5, no. 20, no. 46, no. 53, no. 70, no. 75 and no. The GL content of 79 Uralphanthus was 4.0 to 4.7%. From this, it was confirmed that a larval elephant having a GL content far exceeding the GL value of 2.5% or more stipulated by the pharmacopoeia can be obtained.

  [Example 6]

  In selecting the high GL content rate line of Ural licorice, the external form obtained in Example 2 (the color of the epidermis and the color of the cross section after water washing) and the GL content rate obtained in Example 4 If any relationship can be found in between, the efficiency of selection will be greatly improved. The results of examining this and examining whether it can be applied to system selection are shown in FIGS. 9 (a) and 9 (b). It was.

  As shown in FIGS. 9 (a) and 9 (b), the relationship between the color of the root epidermis and the GL content rate tended to become a higher GL content rate as the root redness became stronger, but notably remarkable. (See FIG. 9 (a)). On the other hand, the relationship between the color of the cross section of the root and the GL content rate is deep, and the GL content rate of individuals whose cross section color of the root is determined to be 5 is as high as 3.12%, determined to be 1 to 3. Significant differences were observed between the individuals (see FIG. 9 (b)). In other words, individuals with a yellow root cross-section have a higher GL content, confirming the previous empirical rules. However, the yellowness of the transverse plane is attributed to the flavonoid compound and is not considered to be directly related to the colorless GL. However, since a high positive correlation is observed between various flavonoid contents and GL contents (see Kondok., Et al., Biol. Pharm. Bull. 30, 1271-1277 (2007)). It was suggested that the lines with high flavonoid production capacity tend to have high GL production capacity. From this, it was suggested that the determination based on the yellowness level of the root crossing plane is not a direct evaluation of the GL content, but is effective as one of the methods for selecting the lineage.

  [Example 7]

  The total amount of glycyrrhizic acid (approximate, GL yield) per individual was obtained by multiplying the raw weight of the ground part of the Uralphanthus obtained in Example 3 by the GL content obtained in Example 4. The calculated results are shown in FIGS.

As shown in FIGS. 6 to 8, the raw weight of the underground part was 824 g and 850 g. 10 and no. 15 had a GL yield of 13.5 to 15.0 g and a GL content of 4.0 to 4.7%. 5, no. 20, no. 46, no. 53, no. 70, no. 75 and no. The GL yield of 79 Uralphanthus was 3.5-8.5 g. From this, it was suggested that these larval elephants are high GL yield larval compared with other raw weights of other larval elephants.
[Example 8]

  The washed sorghum strontium obtained in Example 2 was wrapped in wet newspaper and stored in a refrigerator to obtain a stront for cultivating sorghum of the present invention.

  As described above, the larval elephant of the present invention has an extremely high yield of glycyrrhizic acid, so that it is a raw material for sweeteners such as soy sauce, confectionery, etc. As extremely useful. Moreover, the stron for cultivation of the present invention can cultivate glycyrrhizic acid high-yield uracanzo simply and efficiently, and is excellent in preservability, transportability, marketability and the like.

Claims (7)

Ural licorice No. stored in Hokkaido Research Department, Research Center for Medicinal Plant Resources, National Institute of Medical Science 5, no. 20, no. 46, no. 53, no. 70, no. 75 or No. A glycyrrhizic acid high yielding glycyrrhiz uralensis fisher characterized in that the yield of glycyrrhizic acid in the underground is 3.5 to 15.0 g. Ural licorice No. stored in Hokkaido Research Department, Research Center for Medicinal Plant Resources, National Institute of Medical Science 10 or No. The glycyrrhizic acid high yielding glycyrrhizia urarensis fisher characterized in that the yield of glycyrrhizic acid in the underground is 3.5 to 15.0 g. The glycyrrhiza urarensis fisher according to claim 1 or 2 , wherein the yield of the underground part is 8.5 to 15.0 g. The glycyrrhiz according to any one of claims 1 to 3, wherein the underground content of glycyrrhizic acid is 3.2 to 4.7% and the raw weight of the underground is 150 to 900 g.・ Ularensis Fisher. The glycyrrhiza urarensis fisher according to claim 1 , wherein the underground content of glycyrrhizic acid is 4.0 to 4.7%, and the raw weight of the underground is 150 to 400 g. The glycyrrhiza urarensis fisher according to claim 2 , wherein the underground content of glycyrrhizic acid is 3.2 to 3.7%, and the raw underground weight is 800 to 900 g. A stron for cultivating glycyrrhizic uralensis fisher having a high yield of glycyrrhizic acid, which is used for cultivation of glycyrrhiz uralensis fisher according to any one of claims 1 to 6 .

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