JP5936131B2 - 人工多能性幹細胞の選別方法 - Google Patents

人工多能性幹細胞の選別方法 Download PDF

Info

Publication number
JP5936131B2
JP5936131B2 JP2012548681A JP2012548681A JP5936131B2 JP 5936131 B2 JP5936131 B2 JP 5936131B2 JP 2012548681 A JP2012548681 A JP 2012548681A JP 2012548681 A JP2012548681 A JP 2012548681A JP 5936131 B2 JP5936131 B2 JP 5936131B2
Authority
JP
Japan
Prior art keywords
cells
mirna
region
gene
induced pluripotent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2012548681A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013516982A (ja
JP2013516982A5 (enExample
Inventor
伸弥 山中
伸弥 山中
三千代 小柳
三千代 小柳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyoto University
Original Assignee
Kyoto University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyoto University filed Critical Kyoto University
Publication of JP2013516982A publication Critical patent/JP2013516982A/ja
Publication of JP2013516982A5 publication Critical patent/JP2013516982A5/ja
Application granted granted Critical
Publication of JP5936131B2 publication Critical patent/JP5936131B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2012548681A 2010-01-15 2011-01-17 人工多能性幹細胞の選別方法 Expired - Fee Related JP5936131B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28229510P 2010-01-15 2010-01-15
US61/282,295 2010-01-15
PCT/JP2011/051144 WO2011087154A1 (en) 2010-01-15 2011-01-17 Method for screening induced pluripotent stem cells

Publications (3)

Publication Number Publication Date
JP2013516982A JP2013516982A (ja) 2013-05-16
JP2013516982A5 JP2013516982A5 (enExample) 2014-03-06
JP5936131B2 true JP5936131B2 (ja) 2016-06-15

Family

ID=44304416

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012548681A Expired - Fee Related JP5936131B2 (ja) 2010-01-15 2011-01-17 人工多能性幹細胞の選別方法

Country Status (4)

Country Link
US (1) US20120283130A1 (enExample)
EP (1) EP2524033B1 (enExample)
JP (1) JP5936131B2 (enExample)
WO (1) WO2011087154A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130035241A1 (en) * 2010-04-07 2013-02-07 Institute Of Zoology, Chinese Academy Of Sciences Key genes, micrornas, other non-coding rnas and combination thereof for identifying and regulating the pluripotent status of cells
JP6143265B2 (ja) 2011-07-25 2017-06-07 国立大学法人京都大学 人工多能性幹細胞の選別方法
CN103403158B (zh) * 2011-08-11 2015-01-28 松下电器产业株式会社 检测g-四联体螺旋形成的方法
US20150292040A1 (en) * 2012-01-06 2015-10-15 Kyoto Prefectural Public University Corporation DEVELOPMENT OF SIMPLE DISCRIMINATION METHOD FOR LOW-QUALITY ES CELLS AND iPS CELLS AS INDICATOR OF BIOLOGICAL CLOCK, AND DEVELOPMENT OF CELL EVALUATION METHOD AS INDICATOR OF BIOLOGICAL CLOCK
CN103571929B (zh) * 2012-07-24 2016-05-11 中国科学院动物研究所 无损检测细胞miRNA的表达量及确定细胞类型与状态的方法
EP3452593B1 (en) * 2016-05-02 2020-12-23 Medizinische Hochschule Hannover The incrna meg3 for therapy and diagnosis of cardiac remodelling
JP6718303B2 (ja) * 2016-05-12 2020-07-08 パナソニック株式会社 細胞培養装置および細胞培養方法
KR102193271B1 (ko) * 2016-12-27 2020-12-22 풀스템 코., 엘티디. 3차원 다공성 지지체로부터의 배양세포 회수 방법
EP3743531A1 (en) * 2018-01-22 2020-12-02 Sistemic Scotland Ltd Cell contamination assay

Also Published As

Publication number Publication date
WO2011087154A1 (en) 2011-07-21
EP2524033B1 (en) 2016-04-13
US20120283130A1 (en) 2012-11-08
JP2013516982A (ja) 2013-05-16
EP2524033A1 (en) 2012-11-21
EP2524033A4 (en) 2013-06-26

Similar Documents

Publication Publication Date Title
JP2013516982A (ja) 人工多能性幹細胞の選別方法
Choi et al. A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs
Stadler et al. Characterization of microRNAs involved in embryonic stem cell states
Stadtfeld et al. Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells
CN103459611B (zh) 对多能干细胞的效用和安全性进行表征的功能基因组学研究
Cheng et al. The intrinsic and extrinsic effects of TET proteins during gastrulation
JP6493881B2 (ja) 人工多能性幹細胞の選別方法および血球への分化誘導方法
CN104080907A (zh) 诱导恶性干细胞
US20120164110A1 (en) Differentially methylated regions of reprogrammed induced pluripotent stem cells, method and compositions thereof
US20230340595A1 (en) Use of pluripotent markers to detect contaminating residual undifferentiated pluripotent stem cells
CN107988381B (zh) 筛选诱导的多能干细胞的方法
Stelzer et al. Global analysis of parental imprinting in human parthenogenetic induced pluripotent stem cells
WO2023118050A1 (en) Use of novel markers to detect pluripotent stem cells
JP5807879B2 (ja) 人工多能性幹細胞の選択方法
WO2011046635A1 (en) Differentially methylated regions of reprogrammed induced pluripotent stem cells, method and compositions thereof
CN114438219A (zh) 长链非编码rna作为人源胚胎干细胞标志物的应用
US12571042B2 (en) Markers specific for pluripotent stem cells, and methods of using the same
US11634437B2 (en) Fluorescent compounds specific for pluripotent stem cells and reprogramming-ready cells and methods of using the same
WO2012029316A1 (en) Method for screening differentiation-responsive induced pluripotent stem cell
US20130071919A1 (en) Method of selecting induced pluripotent stem cell
Goldkamp Insights Into Translational Regulation Through tRNA Sequencing, RNA Sequencing, and Ribosome Profiling
WO2026092716A1 (zh) TERT基因在检测iPSC残留中的应用
McPhie et al. SOX10 Expression Levels may be a Critical Mediator of White Matter Dysfunction in Schizophrenia
JP2024510965A (ja) 多能性幹細胞に特異的なマーカー及びその使用方法
JP2013528358A (ja) 細胞多能性を判定又は調整するための鍵遺伝子、ｍｉｃｒｏｒｎａ、他の非コードｒｎａ又はその組合

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140115

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20140115

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150407

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150605

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150924

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151124

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160405

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160428

R150 Certificate of patent or registration of utility model

Ref document number: 5936131

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees