JP5918528B2 - Skin preparation - Google Patents

Description

  The present invention relates to an external preparation for skin having a moisturizing effect and an effect of improving rough skin.

  The stratum corneum of the skin is important as a barrier tissue that prevents the evaporation of moisture from the body and prevents the entry of substances from the outside world. For the purpose of reinforcing the barrier function of the stratum corneum, a substance that promotes the synthesis of ceramide in epidermal cells has been found (see, for example, Patent Document 1) for effectively increasing the amount of ceramide in the stratum corneum. Formulations are being developed, but the effect of improving rough skin with these conventional formulations is still insufficient.

  Moreover, it is known that the application of cosmetics containing ceramides to the skin is effective in reinforcing the barrier function of the stratum corneum (see, for example, Patent Document 2), but efficient penetration into the skin. In terms of sustainability of long-term effects, it is not sufficient. Furthermore, ceramides have the disadvantage that due to solubility problems, it is difficult to blend depending on the dosage form, and blending a large amount is not preferable in terms of stability.

  For these reasons, there is still a need for the development of a skin external preparation that exhibits a better moisturizing effect and a rough skin improving effect.

  On the other hand, porphyran, which is a polysaccharide extracted from seaweed, has properties such as antitumor properties, water retention, and moisture retention, and is therefore attracting attention for use in fields such as pharmaceuticals, foods, and cosmetics. For example, a pack cosmetic composition in which porphyran is formed into a film (see Patent Document 3) has been reported. However, the specific action of porphyran on the epidermis has not been clarified.

JP 2009-107965 A JP-A-62-228048 JP 2008-255066 A

  The object of the present invention is to elucidate the action of a seaweed extract containing porphyran on the epidermis, and to provide a skin external preparation that exhibits an excellent moisturizing effect and a rough skin improving effect based on the newly elucidated action.

  In solving the above problems, the present inventors have conducted intensive studies, and as a result, the seaweed extract containing porphyran such as Susabinori extract has an effect of efficiently promoting ceramide production in epidermal cells. I found. Porphyran, which is a polysaccharide extracted from seaweed, is known to have moisturizing properties. Therefore, blending a seaweed extract containing porphyran into cosmetics has been conventionally performed. However, it has not been known so far that such a seaweed extract acts to efficiently promote ceramide production on epidermal cells, which is a new finding by the present inventors.

  Furthermore, based on this new knowledge, the present inventors used a seaweed extract containing porphyran in combination with an intercellular lipid such as ceramide and / or its related substances, which is remarkably compared with the case of single use. It has been found that there are excellent moisturizing effects and skin roughness improving effects. Further, it has been found that this effect can be obtained with a relatively small amount of porphyran, and the present invention has been completed.

  That is, the first form according to the present invention is (A) 0.1-30% by mass of seaweed extract containing porphyran as a ceramide production promoter, and (B) intercellular lipid and / or its related substance 0.001. It is a skin external preparation containing -5 mass%.

  Moreover, the 2nd form which concerns on this invention is a ceramide production promoter containing the seaweed extract containing porphyran.

  ADVANTAGE OF THE INVENTION According to this invention, the effect | action with respect to the epidermis of the seaweed extract containing porphyran can be clarified, and the skin external preparation which has the outstanding moisturizing effect and the rough skin improvement effect based on the newly elucidated effect can be provided.

It is the figure which showed the ceramide production promotion effect by a Susabiori extract.

[Skin external preparation]
According to the first aspect of the present invention, there is provided a skin external preparation. The skin external preparation according to the first form contains (A) a seaweed extract containing porphyran, and (B) an intercellular lipid and / or a related substance, and preferably (C) a nonionic surfactant. It contains an agent, and further contains other components as necessary.

[(A) Seaweed extract containing porphyran]
(A) A seaweed extract containing porphyran (sometimes referred to simply as “seaweed extract” in the present specification) promotes ceramide production in epidermal cells, and further interacts with intercellular lipids and / or related substances. It is blended in the external preparation for the purpose of improving the moisturizing effect and the effect of improving rough skin by synergistic action. Although direct ceramide supplementation from the outside of the skin with intercellular lipids and / or related substances alone may be inferior in sustainability of the effect, by using a seaweed extract containing porphyran together, the active ingredient porphyran can be reduced. Since it acts on epidermal cells and promotes the natural ceramide production of the skin, it can be expected that the effect of the external preparation for skin will be sustained as a result.

As a seaweed extract, the extract extracted from the seaweed containing porphyran using a well-known method can be used arbitrarily.
Examples of seaweed containing porphyran include red algae and green algae. Examples of red algae include seaweeds belonging to the genus Amanori of the genus Oxenaceae (such as Susabinori), seaweeds belonging to the genus of the genus Oxenaceae (such as Asakusanori), etc. Examples include, but are not limited to, seaweeds (Aonori etc.) belonging to the genus Aonori. These seaweeds may be used alone or in combination of two or more. Among these, it is preferable from the viewpoints of production efficiency and stable supply to use a red algae, Porphyra yezoensis (Bangiaceae), which belongs to the genus Amanori.

The seaweed extract may be extracted from seaweed using an extraction method known to those skilled in the art, or may be obtained as a commercial product. For example, the extract of Susabinori contains water-soluble polysaccharides from all the algae of Susvinori by water, glycerin, propylene glycol, ethanol, butylene glycol, or a water-soluble solvent such as water or a mixture thereof at room temperature or by heating treatment. It can be obtained by extraction and purification.
The obtained extract may be used as it is, or may be used after removing inactive impurities. Alternatively, the concentrated / dried product may be dissolved again in water or a polar solvent and used. Furthermore, the extract is used after subjecting it to purification of decolorization, deodorization, desalting, and fractional extraction by column chromatography within a range that does not impair the desired degree of ceramide production promoting action. You can also
As the commercially available Susavinori extract, “IPF-100K”, “Argefilmer” manufactured by Ichimaru Falcos, or “Pure Porphyra” manufactured by Shiroko Co., Ltd. can be used.

  The content of the seaweed extract in the external preparation for skin can be appropriately set according to various conditions such as the dosage form and intended use, but is 0.1 to 30% by mass as the seaweed extract. 10 mass% is preferable, 0.15-5 mass% is more preferable, 0.2-3 mass% is still more preferable. If the content of the seaweed extract is less than 0.1% by mass, the desired degree of ceramide production promoting effect cannot be obtained, and if it exceeds 30% by mass, it may be unfavorable in terms of use. On the other hand, when the content of the seaweed extract is within a preferable range, an effect of improving skin roughness can be expected while giving a good feeling of use.

  Moreover, as porphyran content in a seaweed extract, 0.001-5 mass% is preferable, 0.01-5 mass% is more preferable, 0.1-3 mass% is still more preferable. When the porphyran content in the seaweed extract is within a preferable range, a rough skin improvement effect can be expected while giving a good feeling of use.

  In addition, the porphyran content in the external preparation for skin is preferably 0.001 to 0.3% by mass, more preferably 0.001 to 0.2% by mass, and still more preferably 0.002 to 0.1% by mass. . When the porphyran content in the external preparation for skin is within the preferable range, an effect of improving rough skin can be expected while giving a good feeling of use.

[(B) Intercellular lipid and / or its related substance]
(B) Intercellular lipids and / or related substances thereof directly replenish intercellular lipid components to the stratum corneum from the outside of the skin, and further improve the moisturizing effect and rough skin improving effect by synergistic action with seaweed extract. For this purpose, it is blended into a skin external preparation.
In addition, intercellular lipid refers to components (ceramide, cholesterol, fatty acid, etc.) present between cells in the stratum corneum, and its related substances refer to substances having a structure and function similar to intercellular lipids. Also included are derivatives of intercellular lipids. In the present specification, intercellular lipids and / or related substances thereof may be simply referred to as “intercellular lipids”.

  Examples of intercellular lipids include, but are not limited to, ceramide, glucosylceramide, ceramide analogs, sphingolipids, fatty acids, cholesterol, cholesterol derivatives, phytosterol derivatives, and the like. Among these, ceramide and phytosterol derivatives are preferable from the viewpoint of the moisturizing effect. These intercellular lipids may be used alone or in combination of two or more.

  Examples of ceramides include ceramide 1, ceramide 1A, ceramide 2, ceramide 3, ceramide 4, ceramide 5, ceramide 6 and ceramide 6II.

  Examples of ceramide analogs include cetyl PG hydroxyethyl palmitamide, cetyl hydroxyprolymph mitamide, trihydroxy palmitamide hydroxypropyl myristyl, trihydroxy palmitamide hydroxypropyl myristyl ether and the like.

  Examples of sphingolipids include N-acetyl dihydrosphingosine, acetyl phytosphingosine, umasphingolipid, caprooil sphingosine, capro oil phytosphingosine, corn sphingoglycolipid, rice bran glycosphingolipid, salicyloyl phytosphingosine, sphingomyelin, sphingo Examples thereof include glycolipids, tetraacetyl phytosphingosine, phytosphingosine, butylresorcinol bissuccinyl phytosphingosine and the like.

  Examples of fatty acids include caprylic acid, capric acid, lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid and the like.

  Examples of cholesterol derivatives include dihydrocholesterol, alkenyl (C16-C18) cholesteryl succinate, cholesteryl isostearate, dihydrocholesteryl isostearate, cholesteryl oleate, dihydrocholesteryl oleate, cholesteryl succinate, cholesteryl dichlorobenzoate, cholesteryl stearate Cholesteryl nonanoate, dihydrocholesteryl nonanoate, cholesteryl hydroxystearate, hydroxy fatty acid (C14-25) cholesteryl, cholesteryl pullulan hexyl dicarbamate, macadamia nut fatty acid dihydrocholesteryl, lauroyl glutamate di (cholesteryl / octyldodecyl), dilauroyl glutamate Cholesteryl / behenyl / octyldodecyl), Rano Down fatty cholesteryl fatty acid (C10-40) (cholesteryl / Ranosuteriru) butyric cholesteryl, dihydrocholesteryl and the like acid.

  Examples of phytosterol derivatives include phytosteryl isostearate, phytosteryl oleate, rice bran fatty acid phytosteryl, dimerlinoleic acid (phytosteryl / isostearyl / cetyl / stearyl / behenyl), dimerlinoleic acid (phytosteryl / behenyl), dimerlinoleic acid Di (isostearyl / phytosteryl), dimer dilinoleic acid dimer dilinoleyl bis (behenyl / isostearyl / phytosteryl), phytosteryl nonanoate, phytosteryl hydroxystearate, sunflower seed oil fatty acid phytosteryl, phytosteryl canola oil fatty acid glycerides, macadamia nut Fatty acid phytosteryl, myristoylmethyl-β-alanine (phytosteryl / decyltetradecyl), laurolyl Glutamic acid di (octyldodecyl / phytosteryl / behenyl), lauroyl glutamate, di (phytosteryl / octyldodecyl), branched fatty acid (C12-31) phytosteryl, butyric phytosteryl, and the like.

  The content of intercellular lipids and the like in the external preparation for skin can be appropriately set according to various conditions such as dosage form and intended use, but is 0.001 to 5% by mass, and 0.001 to 4% by mass. Preferably, 0.005 to 3 mass% is more preferable, and 0.01 to 1 mass% is still more preferable. If the content of intercellular lipids is less than 0.001% by mass, the desired degree of moisturizing effect may not be obtained. If the content exceeds 5% by mass, the spread will be poor and stickiness will occur. May be worse. On the other hand, when the content of intercellular lipids and the like is within a preferable range, a feeling of use is good and an excellent moisturizing effect is obtained.

  Further, in the external preparation for skin, the content ratio of (A) seaweed extract containing porphyran and (B) intercellular lipid and / or its related substance is mass ratio, (A) :( B) = 30000 : 1 to 1:50 is preferable, 10000: 1 to 1:40 is more preferable, and 300: 1 to 1: 5 is still more preferable. When the content ratio of the seaweed extract and the intercellular lipid is within a preferable range, a good feeling of use and a rough skin improvement effect can be expected.

[(C) Nonionic surfactant]
The external preparation for skin of the present invention preferably further contains (C) a nonionic surfactant. In addition to the above-mentioned seaweed extract, intercellular lipids, etc., by adding an appropriate amount of nonionic surfactant, all these components act synergistically, resulting in the moisturizing effect and rough skin of the external preparation for skin. The improvement effect can be further improved. In addition, the inclusion of a nonionic surfactant is advantageous in that it effectively penetrates the active ingredient into the skin and also provides an external preparation for skin that is excellent in stability and safety. .

  Examples of the nonionic surfactant include glycerin fatty acid ester, sorbitan fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene alkyl ether, polyethylene glycol fatty acid ester. , Silicone derivatives, lecithin derivatives, alkyl glyceryl ethers and the like, but are not limited thereto. Among these, glycerin fatty acid ester, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, lecithin derivative, and alkyl glyceryl ether are preferable. These nonionic surfactants may be used individually by 1 type, and may use 2 or more types together. For example, two kinds of glycerin fatty acid ester and polyoxyethylene sorbitan fatty acid ester can be used in combination.

  Examples of glycerin fatty acid esters include glyceryl stearate, glyceryl isostearate, glyceryl oleate, and the like. Examples of sorbitan fatty acid esters include sorbitan monostearate, sorbitan monooleate, sorbitan isostearate, sorbitan sesquioleate, and the like. Examples of polyoxyethylene glycerin fatty acid esters include POE glyceryl monostearate, POE glyceryl monooleate, and the like. Examples of the polyoxyethylene sorbitan fatty acid ester include polysorbate 80, polysorbate 60, polysorbate 40, and the like. Examples of the polyethylene glycol fatty acid ester include PEG monostearate and PEG distearate. Examples of the silicone derivative include polyether-modified silicone and polyglycerin-modified silicone. Examples of lecithin derivatives include lecithin, hydrogenated lecithin, hydroxylated lecithin, hydrogenated lysolecithin and the like. Examples of the alkyl glyceryl ether include isostearyl glyceryl ether.

  Although content of the nonionic surfactant in a skin external preparation can be suitably set according to various conditions, such as a dosage form and an intended purpose, 0.01-5 mass% is preferable, 0.1-4 mass % Is more preferable, and 1-3 mass% is still more preferable. When the content of the nonionic surfactant is within a preferable range, it is advantageous in that the preparation can be stabilized, safety, and an effect of promoting penetration of the active ingredient can be obtained.

  In addition, in the external preparation for skin, the content ratio of (C) nonionic surfactant to the total amount of (A) seaweed extract and (B) intercellular lipid and the like is expressed by mass ratio, [(A) + (B)] :( C) = 3500: 1 to 1: 500 is preferred, 140: 1 to 1:40 is more preferred, and 4: 1 to 1:15 is even more preferred. When the content ratio of the nonionic surfactant to the total amount of the seaweed extract and intercellular lipids is within the preferable range, the feeling of use, stability, and safety are good and the effect of improving skin roughness can be realized. It is advantageous.

[Other ingredients]
The skin external preparation of the present invention includes various components used in normal cosmetics, quasi drugs, pharmaceuticals, etc., for example, oils, powders, surfactants other than nonionic surfactants, adhesives, Resins, amino acids, sugars, organic ultraviolet absorbers, natural plant extract components, solvents, preservatives, sequestering agents, anti-inflammatory components, medicinal components, and the like can be appropriately blended. These other components may be used individually by 1 type, and may use 2 or more types together. Moreover, content of the other component in a skin external preparation can be suitably selected within the range which does not impair the effect of this invention.

  Examples of the oil agent include volatile and non-volatile oil agents, solvents, resins, and the like that are generally used for external preparations for skin, and may be liquid, paste, or solid at room temperature. Examples of oils include cetyl alcohol, stearyl alcohol, behenyl alcohol, isostearyl alcohol, lauryl alcohol, hexadecyl alcohol, octyldodecanol and other higher alcohols, lauric acid, myristic acid, stearic acid, palmitic acid, isostearic acid, undecylenic acid Fatty acids such as glycerin, diglycerin, sorbitol, ethylene glycol, dipropylene glycol, propylene glycol, polyethylene glycol, pentanediol, isopropylene glycol and other polyhydric alcohols, isononyl isononanoate, isotridecyl isononanoate, myristyl myristate, lauric acid Hexyl, decyl oleate, myristyl myristate, hexyl laurate, cetyl ethylhexanoate, olei Decyl acid, isopropyl myristate, hexyl decyl dimethyloctanoate, glyceryl monostearate, glyceryl trioctanoate, glyceryl tri-2-heptylundecanoate, diethyl phthalate, ethylene glycol monostearate, octyl oxystearate, diisostearate Esters such as stearyl, hydrocarbons such as liquid paraffin, paraffin, petrolatum, squalane, lanolin, reduced lanolin, liquid lanolin, carnauba wax, candelilla wax, ceresin, ozokerite, microcrystalline wax, wax, mink oil, cocoa butter, Oils and vegetable oils such as palm oil, palm oil, camellia oil, sesame oil, macadamia nut oil, meadow home oil, jojoba oil, castor oil, olive oil, methyltrimethicone, dimethylpolysiloxax , Methyl phenyl polysiloxane, decamethylcyclopentasiloxane, silicone gel, trimethylsiloxysilicic acid, silicone PTV rubber and other silicone compounds, polyethylene wax, ethylene / α-olefin / co-oligomer, 1,3-dimethyl-butyl ether, etc. It is done.

  Examples of powders include red 104, red 102, red 226, red 201, red 202, yellow 4, blue 1, black 401, yellow 4 aluminum lake, yellow 5 Rake dyes such as aluminum lake, yellow No. 203 barium lake, nylon powder, silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, silicone elastomer spherical powder, polyethylene powder, etc. Polymers such as yellow iron oxide, red iron oxide, black iron oxide, chromium oxide, carbon black, ultramarine, bitumen and other colored pigments, zinc oxide, titanium oxide, cerium oxide and other white pigments, talc, mica, sericite, kaolin, Extender pigments such as plate-like barium sulfate, pearl pigments such as titanium mica, sulfur Examples include metal salts such as barium, calcium carbonate, magnesium carbonate, aluminum silicate, magnesium silicate, inorganic powders such as silica and alumina, bentonite, smectite, boron nitride, fine particle titanium oxide, fine particle zinc oxide, etc. Use of known surface treatments such as N-acylated lysine treatment, amino acid treatment, hydrophilic polymer treatment, oil agent treatment, silicone treatment, metal soap treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment, etc. It is also possible to do. There are no particular restrictions on these powder shapes (spherical, rod-like, needle-like, plate-like, irregular shape, flake-like, spindle-like, etc.). The size of the powder is preferably in the range of 5 nm to 100 μm, more preferably 10 nm to 25 μm. These powders may be processed alone or may form a mixture and be processed together. Moreover, it is also possible to process what adjusted the color of the mixture to skin color etc. Furthermore, it is possible to obtain a treated powder having an ultraviolet protection function by using an ultraviolet scattering component such as fine particle titanium oxide or fine particle zinc oxide.

  As surfactants other than nonionic surfactants, for example, anionic surfactants, cationic surfactants, and amphoteric surfactants can be used. More specifically, fatty acid soap, α-acyl sulfonate, N-acyl amino acid, N-acyl taurate, N-acyl glutamate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate Anionic surfactants such as salts, alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, alkylamide phosphates, alkyloyl alkyltaurine salts, sulfosuccinates, perfluoroalkyl phosphate esters, alkyltrimethyl chlorides Ammonium, outeraryltrimethylemonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltobromide Cationic surfactants such as methylammonium, amphoteric surfactants such as carboxybetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amidoamine type, etc. Can be mentioned.

  Examples of adhesives and resins include sodium polyacrylate, cellulose ether, alginate, carboxyvinyl polymer, ethylene / acrylic acid copolymer, vinyl pyrrolidone polymer, vinyl alcohol / vinyl pyrrolidone copolymer, nitrogen substituted acrylamide type Polymers, cationic polymers such as cationic gar gum, POE / POP copolymers, polyvinyl alcohol, pullulan, deoxyribonucleic acid and salts thereof, acidic mucopolysaccharides and salts thereof such as chondroitin sulfate, tamarind seed polysaccharide, tuberose polysaccharide, agar, Xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, locust bean gum, crystalline cellulose, arabinogalactan, caraya gum, tragacanth gum, algin , Hyaluronic acid and its salts, albumin, casein, curdlan, gellan gum, dextrin, cyclodextrin, dextran, cellulose, hydroxyethylcellulose, carboxymethylcellulose, hydroxypropylmethylcellulose, polyethyleneimine, highly polymerized polyethyleneglycol, cationized silicone polymer, synthesis Examples include latex.

  As amino acids, L-glutamic acid, L-leucine, L-serine, sodium L-aspartate, L-threonine, DL-alanine, L-isoleucine, L-phenylalanine, L-arginine, L-proline, L-tyrosine , L-histidine hydrochloride, lysine hydrochloride, glycine, N-acetyl-L-glutamic acid, sodium PCA, trimethylglycine, γ-polyglutamic acid and the like.

  Examples of the saccharide include galactose, mannose, maltose, fructose, sucrose, xylose, sorbitol, xylitol, mannitol and the like.

  Examples of organic ultraviolet absorbers include 2-methoxyhexyl paramethoxycinnamate, 2-ethylhexyl paradimethylaminobenzoate, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfate, 2,2′-dihydroxy-4-methoxybenzophenone, p-methoxyhydrocinnamic acid diethanolamine salt, paraaminobenzoic acid (hereinafter abbreviated as PABA), homomenthyl salicylate, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano -3,3-diphenyl acrylate, octyldimethyl PABA, octyl salicylate, 2-phenyl-benzimidazole-5-sulfuric acid, triethanolamine salicylate, 3- (4-methylbenzylidene) camphor, 2,4-dihydroxy Nzophenine, 2,2 ′, 4,4′-tetrahydroxybenzophenone, 2,2′-dihydroxy-4,4′-dimethoxybenzophenone, 2-hydroxy-4-N-octoxybenzophenone, 4-isopropyldibenzoylmethane, Examples include butylmethoxydibenzoylmethane, 4- (3,4-dimethoxyphenylmethylene) -2,5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and the like.

  Examples of natural plant extracts include Ashitaba extract, Avocado extract, Achacha extract, Altea extract, Arnica extract, Aloe extract, Apricot extract, Apricot kernel extract, Ginkgo biloba extract, Fennel extract, Turmeric extract, Oolong tea extract, Ages extract , Echinacea leaf extract, Ogon extract, Oat extract, Auren extract, Barley extract, Hypericum extract, Adrianthus extract, Dutch mustard extract, Olive leaf extract, Orange extract, Seawater extract, Seaweed extract (other than seaweed extract including porphyran) , Hydrolyzed elastin, hydrolyzed wheat powder, hydrolyzed silk, chamomile extract, carrot extract, kawara mugi extract, licorice extract, calcaret extract, oyster extract, kiwi extract, kina extract, cue Extract, guanosine, gardenia extract, Kumazasa extract, Clara extract, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, tea extract, yeast extract, burdock extract, rice bran extract, rice germ oil, comfrey Extract, Collagen, Cowberry extract, Saishin extract, Psycho extract, Saitai extract, Salvia extract, Salmon extract, Sasa extract, Hawthorn extract, Salamander extract, Shiitake extract, Giant extract, Shikon extract, Perilla extract, Linden extract, Shimotake extract, Peonies extract , Camphor root extract, birch extract, Japanese horse chestnut extract, horseshoe extract, hawthorn extract, elderberry extract, sawtooth Extract, mint extract, sage extract, mallow extract, sensu extract, senbu extract, soybean extract, taisu extract, thyme extract, tea extract, clove extract, chigaya extract, chimpi extract, suzuki extract, eucalyptus extract, tonin extract, spruce extract, dokudami extract , Tomato extract, natto extract, carrot extract, garlic extract, wild rose extract, hibiscus extract, bacmond extract, lotus extract, parsley extract, honey, hamamelis extract, parietalia extract, cassava extract, bisabolol, loquat extract, dandelion extract, fukinotou extract, Bukuryo extract, butcher bloom extract, grape extract, propolis, loofah extract, safflower extract, peppermint extract, bodyfish Juju extract, button extract, hop extract, pine extract, maroonnier extract, mizubasho extract, mugwort extract, melissa extract, peach extract, cornflower extract, eucalyptus extract, yukinoshita extract, yuzu extract, yokoinin extract, mugwort extract, lavender extract, apple extract, lettuce extract, Examples include lemon extract, lotus extract, rose extract, rosemary extract, roman chamomile extract, royal jelly extract and the like.

  Examples of the solvent include purified water, cyclic silicone, ethanol, light liquid paraffin, lower alcohol, ethers, LPG, fluorocarbon, N-methylpyrrolidone, fluoroalcohol, volatile linear silicone, and next-generation fluorocarbon. .

  Examples of preservatives include benzoic acid, sodium benzoate, undecylenic acid, salicylic acid, sorbic acid or salts thereof, tehydroacetic acid or salts thereof, methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, isopropyl paraoxybenzoate Organic acids such as butyl paraoxybenzoate, isobutyl paraoxybenzoate, benzyl paraoxybenzoate and derivatives thereof, isopropylmethylphenol, chlorhexidine gluconate, cresol, thymol, parachlorophenol, phenylethyl alcohol, phenylphenol, sodium phenylphenol, Phenoxyethanol, phenoxydiglycol, phenols such as phenol and benzyl alcohol, stearyltrimethylenmonium chloride, cetyl chloride Quaternary ammonium salts such as methylammonium, cetylpyridinium chloride, benzalkonium chloride, benzethonium chloride, methylbenzethonium chloride, laurylmethylammonium chloride, alkylisoquinolium bromide, domifene bromide, tea extract, hinokitiol, apple extract, rose Plant extracts such as mary extract, chloramine T, chlorhexidine, zinc pyrithione and the like can be mentioned.

  Examples of the sequestering agent include ethylenediaminetetraacetic acid disodium, edetic acid, edetic acid disodium, etidronic acid and the like.

  Examples of the anti-inflammatory component include dipotassium glycyrrhizinate, stearyl glycyrrhetinate, allantoin, tocopherol acetate, tocopherol, and azulene.

  As a medicinal ingredient, vitamin A oil, retinol, riboflavin, pyridoxine hydrochloride, L-ascorbic acid, magnesium L-ascorbate phosphate, L-ascorbic acid monopalmitate, L-ascorbate dipalmitate, L- Examples include ascorbic acid-2-glucoside, pantothenate, vitamin D2, cholecalciferol, and the like.

[Dosage form, manufacturing method, etc.]
The external preparation for skin of the present invention can be produced in an arbitrary dosage form such as a solubilization system, an emulsification system, a powder dispersion solubilization system, a powder dispersion emulsification system, and a powder dispersion oil system according to a conventional method. In addition, it is preferable to adjust the skin external preparation of this invention to pH 2-11, especially pH 3-9. The topical skin preparation of the present invention includes skin care products such as lotions, emulsions, creams and cosmetic oils, as well as makeup cosmetics such as foundations, powders, lipsticks, blushers, eye shadows, nail enamels, tablets, capsules and granules. It can be suitably used as a product such as a bath agent in the form of an agent, powder, liquid or the like.

  In the external preparation for skin according to the first form, as described above, a desired effect can be obtained with a relatively small amount of porphyran. Intercellular lipid components such as ceramide are directly supplied to the epidermis from the outside of the skin by the action of intercellular lipids, and ceramide production by the seaweed extract containing porphyran enhances the efficiency of ceramide production in epidermal cells. Promoted. Therefore, it is considered that an excellent moisturizing effect and rough skin ameliorating effect can be achieved even with a small amount of porphyran due to the synergistic effect of ceramide supply from the outside of the skin and promotion of ceramide production from the inside of the skin.

[Ceramide production promoter]
Moreover, this invention is a ceramide production promoter according to 2nd form. The ceramide promoter according to the second embodiment contains a seaweed extract containing porphyran. As the ceramide production promoter, the seaweed extract containing the above porphyran may be used by acting on epidermal cells alone, or may be used by acting on epidermal cells together with other optional components. . Moreover, there is no restriction | limiting in particular in content and dosage form of the seaweed extract in a ceramide production promoter, According to the objective, it can select suitably. Since the ceramide production promoter has an excellent ceramide production promotion action on epidermal cells, it can be suitably used as one active ingredient in, for example, a skin external preparation composition.

  The present invention will be described more specifically with reference to the following examples. However, the present invention is not limited to these examples.

<1. Preparation of ceramide production promoter and examination of ceramide production promoting action>
The extract of Susabinori obtained by extracting all the algae of Suprabinori [Porphyra yezoensys (Bangiaceae)] with water was used as a ceramide production promoter, and the ceramide production promotion action was examined as follows. In addition, porphyran content in the obtained Susabinori extract was about 1 mass%.

(1) Cell culture Using a 60 mm dish, normal human epidermal keratinocytes (NHEK (F), manufactured by KURABO) were cultured at 37 ° C. and 5% in a culture solution (trade name: Epilife-KG2, manufactured by KURABO). Cultivated with CO ₂ until confluent. The culture supernatant is aspirated, and the medium prepared so that the concentration of the Susabinori extract prepared above is 0.01 w / v% in terms of solid content or the control solution (30% butylene glycol aqueous solution) is 0.01 v / v%. And cultured for 7 days. The medium was replaced with the prepared medium every other day.

(2) Lipid extraction and ceramide quantification After culturing, the cells were washed with PBS, and the cells were collected using a scraper. Lipids were extracted from the collected cells by the Bligh and Dyer method. The extracted organic phase was transferred to a glass tube and solidified with nitrogen, and then redissolved with chloroform: methanol = 2: 1 (volume ratio) to obtain a lipid sample. The prepared lipid sample was horizontally developed twice by thin layer chromatography (HPTLC Silica gel 60, manufactured by MERK) using chloroform: methanol: acetic acid = 190: 9: 1 (volume ratio). Thereafter, a 10% copper sulfate solution was sprayed and baked on a hot plate to quantitate ceramide. The value of the amount of ceramide at the time of sample addition when the amount of ceramide at the time of control solution addition was 100 was defined as the ceramide production promotion rate.

  As a result, the ceramide production promotion rate of the Susabinotori extract was 183.4% (FIG. 1). From this, it was recognized that the amount of ceramide produced was increased in the system to which the extract of Susabinotori was added compared to the control system. Therefore, it was revealed that the seaweed extract containing porphyran has an excellent ceramide production promoting action.

<2. Preparation of external preparation for skin>
The water phase components 10 to 13 and the component 15 dissolved by heating at 80 ° C. were added to the oil phase components 2 to 9 heated and dissolved at 80 ° C., and the mixture was stirred and emulsified. After emulsification, the mixture was cooled to room temperature with stirring, and component 14 was added and further stirred. After stirring, component 1 was added and stirred to prepare a skin external preparation. (Refer to Tables 1-3 for each component number. The unit of each component amount in the table is mass%.)

<3. Examination of moisturizing effect and skin roughness improving effect>
A skin external preparation was actually used for 10 women (25 to 50 years old) who complained of rough skin or dry skin for some reason, and examined moisturizing effect and rough skin improving effect. For the subjects, while observing the skin condition every day, after preparing the skin with the skin lotion that they normally use after washing in the morning and night, the skin external preparations shown in Tables 1 to 3 prepared above (implemented) Appropriate amounts of Examples 1-8 and Comparative Examples 1-4) were applied. Application was continued for one month, the skin conditions at the start and end of the test were compared, and the following items were evaluated.
There are four evaluation items: “dry skin is moist”, “texture has become finer”, “smooth skin has improved”, and “morning feeling of the next morning”. The test results are shown in the table according to how many out of 10 responded that each item improved. In addition, about the "moist feeling of the skin of the next morning", after continuing the application for one month, the skin condition of the next morning on the day when the application was stopped was evaluated.

From the results of Tables 1 to 3, it can be said that Comparative Example 2 and Comparative Example 3 containing Susabinori extract or ceramide alone have sufficient improvement effects for each item compared to Comparative Example 1 containing none. There wasn't.
On the other hand, in Examples 1 to 4 in which Susabinori extract and ceramide were used in combination, the results showed a remarkable improvement effect for each item. Moreover, the same improvement effect was recognized about each item also in Examples 5-8 which used the Susabinori extract and lauroyl glutamate di (phytosteryl / octyldodecyl) together. In addition, although the result is not shown in the table, the same improvement effect was recognized for each item when the Susabinori extract and phytosteryl oleate were used in combination.

  Moreover, when Example 3 was compared with Example 4, in Example 3, the remarkable improvement effect was recognized about each item. This result shows that the moisturizing effect and the rough skin improving effect of the external preparation for skin are further enhanced by adding an appropriate amount of a nonionic surfactant together with the extract of susbinori and ceramide.

  From these results, the combined use of (A) a seaweed extract containing porphyran and (B) an intercellular lipid and / or its related substance significantly improves the moisturizing effect and the rough skin improving effect of the external preparation for skin. Became clear. It was also revealed that these effects can be further enhanced by using an appropriate amount of (C) a nonionic surfactant in combination.

  Since the external preparation for skin of the present invention can exhibit an excellent moisturizing effect and an effect of improving rough skin, it can be suitably used, for example, for cosmetics intended to make the skin healthier.

Claims (5)

(A) 0.2-3 mass% of seaweed extract containing porphyran as a ceramide production promoter;
(B) ceramide, lauroylglutamate di (phytosteryl / octyldodecyl), or intercellular lipid that is phytosteryl oleate and / or its related substance 0.005 to 3 % by mass ;
(C) 0.1 to 4% by mass of at least one nonionic surfactant selected from the group consisting of glycerin fatty acid ester, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, lecithin derivative, and alkyl glyceryl ether An external preparation for skin containing <br/>. (A) The skin external preparation according to claim 1, wherein the seaweed extract containing porphyran is a Susavinori extract. (C) The external preparation for skin according to claim 1 or 2, wherein the nonionic surfactant is glyceryl stearate and polysorbate 80. (C) Content of nonionic surfactant is 1-3 mass%, The skin external preparation in any one of Claims 1-3. The skin external preparation according to any one of claims 1 to 4, wherein the content of porphyran in the external skin preparation is 0.002 to 0.1 mass%.

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