JP2012077044A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic material for skin aging prevention, excellent in the effect of skin aging prevention (effect of improvement in skin roughening, improvement in keratin, promotion in turnover, etc.).SOLUTION: Calcium saccharide phosphate is added to a skin care preparation of a cosmetic or a quasi drug to obtain a preparation for skin aging prevention for external use with the excellent anti aging effect.

Description

The present invention provides a skin external preparation excellent in anti-aging effects such as turnover promoting action, keratinocyte differentiation promoting action, tight junction formation promoting action, cell activation action, hyaluronic acid production promoting action by containing phosphorylated saccharide calcium About.

Skin is roughly divided into a structure consisting of an outer thin epidermis (epithelial tissue) and a thicker dermis (connective tissue) underneath. The epidermis forms the outermost layer of the body, protects the living body from the outside world, and plays a role in preventing internal moisture and nutrients from leaking out to the outside world. The dermis is a connective tissue with a structure in which mainly fibroblasts, collagen fibers, elastic fibers, proteoglycans, etc. are spread in a three-dimensional manner, giving the skin strength, extensibility and elasticity. Bring. The skin condition is maintained by the epidermis and dermis both maintaining their healthy tissue structure and physiology.

For example, when the metabolic function of epidermal cells decreases due to aging and the turnover speed of the skin slows down, it may cause skin aging and rough skin, or horny layer cells that fall off the stratum corneum surface may stay. It is known that melanin discharged from melanocytes to epidermal cells is not smoothly discharged, which causes pigmentation and dull skin. (Refer to Non-Patent Document 1) In addition, when the proliferation and differentiation of keratinocytes are suppressed due to aging or disease, the supply of keratinocytes from the lower layer decreases, the turnover of the stratum corneum is delayed, and the stratum corneum thickens. Further, it is known that the moisture content is reduced and the moisture retaining function and the barrier function by the stratum corneum are lowered. Until now, it has been thought that only the outermost stratum corneum is responsible for the barrier function of the skin. Recently, however, an adhesive device “Tight Junction” that seals between cells has also been developed. It has been found to be important for the barrier function. (See Non-Patent Document 2) Furthermore, it has also been found that tight junctions acting as a permeation barrier for the epidermis are destroyed by ultraviolet exposure or the like and the permeation barrier function of the skin is lowered. (See Non-Patent Document 3) In addition, aging causes a decrease in fibroblasts and hyaluronic acid in the dermis and a degeneration such as collagen and elastin, which causes skin aging and rough skin symptoms such as wrinkle formation and reduced elasticity. It is known. In order to prevent or improve these progressions, many skin external preparations have been proposed.

For example, as a conventional turnover promoter, as a koji extract (see Patent Document 1), as a keratinocyte differentiation promoter, as a Ceylon tetsuboku seed extract (see Patent Document 2), as a tight junction formation promoter, a kingwing extract (Refer to patent document 3) As a cell activator, psyllium seed extract (refer to patent document 4) As a hyaluronic acid production promoter, an amacha extract (refer to patent document 5) is known. Moreover, phosphorylated saccharide calcium (see Patent Document 6), which is an enzyme degradation product of potato starch, is a remineralization promoting effect on initial caries (see Non-Patent Document 4), a moisturizer and a collagen production promoter for fibroblasts. (See Patent Document 7).

JP 2007-314522 A JP 2005-213187 A JP 2009-269900 A JP2009-234976 JP2007-51091A JP-A-8-104696 Japanese Patent Laid-Open No. 18-249077 Advances and Future Prospects of Cosmetics Usability Evaluation Technology Page 210 Yakuji Nippo J. Cell Biol, 117: 1050-1058 (2002) Archives of Dermatological Reserch, 300: 61-68 (2008) J. Apply. Glycosci, 51, 129-134 (2004)

Many different substances and skin compositions that improve the skin condition have been reported, but they all focus on improving the symptoms of either the epidermis or the dermis, both the epidermis and the dermis. There has been a demand for a novel substance and a skin-improving agent that can improve skin and provide a comprehensive and high skin-improving effect.

In order to achieve the above object, the present invention provides, for example, the following means:

A skin external preparation characterized by containing phosphorylated saccharide calcium as a turnover promoting component.

A skin external preparation characterized by containing phosphorylated saccharide calcium as a keratinocyte differentiation promoting component.

A skin external preparation characterized by containing phosphorylated saccharide calcium as a tight junction formation promoting component.

A skin external preparation characterized by containing phosphorylated saccharide calcium as a cell activation component.

A skin external preparation characterized by containing phosphorylated saccharide calcium as a hyaluronic acid production promoting component.

It is known that phosphorylated saccharide sugar calcium has a dental remineralization promoting effect, a calcium absorption promoting effect, and a collagen production promoting effect. However, it has anti-aging effects such as epidermal turnover promoting action, epidermal keratinocyte differentiation promoting action, epidermal tight junction formation promoting action, skin fibroblast activation action, hyaluronic acid production promoting action from skin fibroblasts, etc. Was not known at all. In addition, anti-aging substances so far have focused only on improving the symptoms of either the epidermis or dermis, but phosphorylated saccharide calcium improves the symptoms of both the epidermis and dermis and is comprehensive and High anti-aging effect can be brought about.

The phosphorylated saccharide calcium of the present invention is an epidermal turnover promoting component, an epidermal keratinocyte differentiation promoting component, an epidermal tight junction formation promoting component, a skin fibroblast activation component, and hyaluronic acid production from skin fibroblasts. A skin external preparation having a skin improvement effect can be obtained by blending into a base material of various forms used for normal skin external preparations and formulating them as an accelerating action component according to a conventional method.

The phosphorylated saccharide calcium used in the present invention comprises a saccharide moiety and a phosphate group calcium salt. As used herein, the term “phosphorylated sugar” refers to a sugar having at least one phosphate group in the molecule. As used herein, the term “phosphorylated saccharide calcium” refers to a calcium salt of phosphorylated saccharide. In the phosphorylated saccharide calcium used in the present invention, the sugar moiety is a glucan having a polymerization degree of 2 to 8, and the number of phosphate groups is 1 to 2. The number of calcium ions in the phosphorylated saccharide calcium is 1 to 2, and preferably the number of phosphate groups in the phosphorylated saccharide calcium is equal to the number of phosphate ions.

The phosphorylated saccharide calcium used in the present invention may be used as a pure one type of compound or as a mixture of a plurality of types. The phosphorylated saccharide calcium used in the present invention is preferably a phosphorylated saccharide calcium described in JP-A-8-104696. When produced according to the method described in JP-A-8-104696, a mixture of a plurality of types of phosphorylated saccharide calcium is obtained. The mixture may be used as it is, or after separation into a pure compound, only one kind of compound may be selected and used. Phosphorylated saccharide calcium exhibits excellent performance both when used as a mixture and as a mixture.

The phosphorylated saccharide calcium can be produced, for example, by phosphorylating a known saccharide to obtain a phosphorylated saccharide, and then converting the phosphorylated saccharide into a calcium salt. A method for producing phosphorylated saccharide is described in JP-A-8-104696. Phosphorylated sugar calcium powder is also sold as phosphorylated oligosaccharide calcium by Ezaki Glico Co., Ltd.

The content of phosphorylated saccharide calcium in the external preparation for skin of the present invention is preferably 0.01 to 10% by mass (hereinafter simply referred to as “%”), more preferably 0.05 to 5% as a dry solid content. More preferably, it is 0.1 to 3%. Within this range, phosphorylated saccharide calcium can be blended stably, and high epidermal turnover promoting effect, epidermal keratinocyte differentiation promoting effect, epidermal tight junction formation promoting effect, skin fibroblast activation effect In addition, it can exert an effect of promoting hyaluronic acid production from dermal fibroblasts.

When the phosphorylated saccharide calcium used in the present invention is further combined with other medicinal ingredients, a more effective skin external preparation composition can be obtained. Other medicinal ingredients are those selected from moisturizers, antioxidants, cell activators, whitening agents, and UV inhibitors, and specific medicinal agents include those shown below.

First, as a humectant, glycerin, 1,3-butylene glycol, protein or a derivative or hydrolyzate thereof and a salt thereof (collagen, elastin, keratin, etc.), mucopolysaccharide and a derivative thereof (hyaluronic acid, hydrolyzed hyaluronic acid, Chondroitin sulfate, etc.), amino acids and derivatives thereof (histidine, serine, glycine, theanine, aspartic acid, arginine, lysine, pyrrolidonecarboxylic acid, N-methyl-L-serine, etc.), saccharides (sorbitol, erythritol, trehalose, inositol, glucose) , Xylitol, N-acetylglucosamine, raffinose, sucrose and derivatives thereof, dextrin and derivatives thereof, honey, etc.), D-pantenol and derivatives thereof, glycolipids, ceramides, sugar ceramides, amateur extracts, a -Monde extract, Ashitaba extract, Avocado extract, Altea extract, Arnica extract, Hot spring water, Chinpi extract, Aloe extract, Usvenia oyster extract, Scarabae extract (Ogon) extract, Auren extract, Hypericum extract, Adriatic extract , Onionis extract, chamomile extract, oat extract, glabrizine, glabrene, liquiritin, isoliquiritin and licorice extract containing these, water-soluble and oil-soluble licorice extract, raspberry extract, Himalayan raspberry extract, honeysuckle ) Extract, Quince Seed (Quince) Extract, Clara (Kujin) Extract, Gardenia Extract, Kumazasa Extract, Water-soluble Chlorophyll, Maple Leaf Extract, Yuzu Extract, Grapefruit Extract, Watercress Extract, Gentiana (Gentian) Extract, Gentian extract, Burdock extract, Sesame extract, Wheat extract, Wheat germ extract, Comfrey extract, Saisin extract, Cactus extract, Salmon extract, Salvia (Sage) extract, Hawthorn extract, Giant extract, Perilla extract, Japanese apricot extract, Citrus extract, Peonies extract, Ginger extract, Ginger extract, Birch extract, Prunus extract (Peppermint, Green lily, Spearmint, etc.) Benitachaoi) extract, Japanese horsetail extract, Senkyu extract, Mulberry extract, Tachikaku (Thyme) extract, Camellia extract, Japanese persimmon extract, Red pepper extract, Corn extract, Dokudami extract, Hibiscus Extract, white willow extract, g Mentilla extract, parsley extract, pearl barley (witch hazel) extract, hamameris (witch hazel) extract, rose extract, hinoki extract, sunflower extract, sunflower dandelion extract, butcher's bloom extract, grape extract, prune ( (Plum) extract, loofah extract, avocado extract, okra extract, bodaiju extract, moon peach extract, button (button pi) extract, hop extract, jojoba leaf extract, jojoba oil, macadamia nut oil, olive oil, apricot kernel oil, permanent Oil, safflower oil, sunflower oil, avocado oil, camellia oil, almond oil, sesame oil, sesame oil, borage (borage) oil, cocoa butter, shea fat, pine extract, maroni extract, mucrot extract, mucin, purple (Sicon) extract, Meadow home oil, Melissa extract, cornflower Extract, Yukinoshita extract, Lily extract, Lime extract, Lavender extract, Apple extract, Gentian (Ryutan) extract, Phospholipid (derived from soybean, egg yolk, etc.), Astragalus extract, Walnut extract (Jiuyu) extract , Tea (Oolong tea, green tea, black tea, etc.) extract, Mokutsu extract, urea, Rakan fruit extract, white jellyfish polysaccharide, seaweed extract (comb, macombe, wakame, hijiki, hibamata, sujime, trorocomb, kajime, tsuruarame, chigaiso Brown algae such as Amanda, Honda, Giant Kelp; Red algae such as Tengusa, Greater Rhinoceros, Giraffe Rhinoceros, Tsunomatsu, Sugiori, Usubanori, Asakusanori, Matsunori, Tokamatsu, Funori, Ogonori, Kamensou, Igis, Egonori; Coccus, Anaaosa, Kawanori, Ma Green algae such as limo, shiogusa, casanori, ftjuzumo, tamajuzumo, human exothera, and blue-green algae; cyanobacteria such as spirulina), pear juice fermented liquid, hibiscus fermented liquid, and rice fermented liquid such as rice fermented liquid. By adding these moisturizers, more epidermal turnover promotion, epidermal keratinocyte differentiation, epidermal tight junction formation, skin fibroblast activation, hyaluronic acid production from skin fibroblasts The effect can be exerted. (In addition, the indication in parentheses in the extract indicates the plant alias or the name of the herbal medicine. The same applies hereinafter.)

Antioxidants include vitamin E and its derivatives (dl-α (β, γ) -tocopherol, dl-α-tocopherol acetate, nicotinic acid-dl-α-tocopherol, linoleic acid-dl-α-tocopherol, succinic acid. Tocopherols such as acid dl-α-tocopherol and derivatives thereof, ubiquinones, etc., vitamin A and derivatives thereof (retinol and retinol palmitate, derivatives such as retinol acetate, retinal such as dehydroretinal and derivatives thereof), carotenoids (carotene) Lycopene, astaxanthin, etc.), vitamin B and its derivatives (thiamine hydrochloride, thiamine sulfate, riboflavin, riboflavin acetate, pyridoxine hydrochloride, pyridoxine dioctanoate, flavin adenine nucleotide, cyanocobalamin, folic acid, ni Nicotinic acid amide, nicotinic acid benzyl, etc., choline, etc.), vitamin C and its derivatives (L-ascorbic acid phosphate, L-ascorbic acid sulfate, dipalmitic acid L-ascorbyl, tetraisopalmitic acid L) -Ascorbyl, ascorbic acid glucoside, etc.), vitamin D and its derivatives (ergocalciferol, cholecalciferol, dihydroxystannal etc.), mevalonolactone, rutin and its derivatives, thiotaurine, taurine, hydroquinone and their derivatives (arbutin, α-arbutin) ), Histidine, catechin and derivatives thereof, grabrizine, glabrene, liquiritin, isoliquiritin and licorice extract containing these, glutathione and derivatives thereof, gallic acid and derivatives thereof, picno Genol, spruce extract, evening primrose extract, cucumber extract, caquette extract, gentian (gentian) extract, genocarpus extract, cholesterol and its derivatives, hawthorn extract, peonies extract, superoxide dismutase, ginkgo biloba extract (Ougon) extract, carrot extract, maika (maikai, hermanus) extract, sunpens (kawara ketsumei) extract, tormentilla extract, parsley extract, grape extract, button (button pi) extract, mannitol, mokka (bokeh) Extract, Melissa extract, Yashajitsu (Yasha) extract, Yukinoshita extract, Rosemary (Mannenrou) extract, Bilberry extract, Bilberry leaf extract, Stevia extract, Lettuce extract, Tea extract (Oolong tea, Beni Tea, green tea, etc.), microbial fermentation metabolites, seaweed extract, ganoderma extract, eggshell membrane extract, placenta extract, Rahan fruit extract and the like.

Examples of cell activators include vitamin A and derivatives thereof (derivatives such as retinol and retinol palmitate, retinol acetate; derivatives such as retinal and dehydroretinal), vitamin C and derivatives thereof (L-ascorbic acid phosphate, L- Ascorbic acid sulfate, L-ascorbyl dipalmitate, L-ascorbyl tetraisopalmitate, ascorbyl glucoside, etc.), vitamin B and its derivatives (thiamine hydrochloride, thiamine sulfate, riboflavin, riboflavin acetate, pyridoxine hydrochloride, pyridoxine di) Octanoate, flavin adenine nucleotide, cyanocobalamin, diisopropylamine dichloroacetic acid, folic acid, nicotinic acid amide, nicotinic acid benzyl and other nicotinic acids, choline, etc.), coenzyme Q10 Ribonucleic acid and salts thereof, deoxyribonucleic acid and salts thereof, α- and γ-linolenic acid, xanthine and derivatives thereof (such as caffeine), almond extract, asparagus extract, amino acids and derivatives thereof (serine, glutamic acid, theanine) , Hydroxyproline, pyrrolidone carboxylic acid, γ-aminobutyric acid, γ-amino-β-hydroxybutyric acid, etc.), apricot extract, ginger extract, ginkgo biloba extract, docosahexaenoic acid and its derivatives, eicosapentaenoic acid and Its derivatives, yellowfin extract, barley extract, kiwi extract, cucumber extract, citric acid, succinic acid, malic acid, shiitake extract, sugi extract, assembly extract, soybean extract, Jujube extract, camellia extract, red pepper extract, Milkweed extract, wheat germ extract, tomato extract, garlic extract, carrot extract, hinokitiol, beak extract, grape seed oil, beech extract, star fruit extract, peach extract, eucalyptus extract, lily extract , Orchid extract, lettuce extract, lemon extract, rosemary (mannenrou) extract, malt root extract, animal-derived extract (mollusk and other mollusc extracts, shell extract, shell meat extract, pearl protein extract Liquid, fish extract, chicken crown extract, royal jelly, silk protein and its degradation product, placenta extract, serum deproteinized extract), lactoferrin or its degradation product, microorganism-derived extract (yeast extract), microorganism fermentation solution (Derived from lactic acid bacteria, bifidobacteria, etc.), ganoderma extract and the like.

Examples of whitening agents include vitamin C and derivatives thereof (L-ascorbic acid phosphate, L-ascorbic acid sulfate, dipalmitic acid L-ascorbyl, tetraisopalmitic acid L-ascorbyl, ascorbic acid glucoside, etc.), placenta extract , Dipotassium glycyrrhizinate, glabrizine, glabrene, liquiritin, isoliquiritin and licorice extract, yakuinin extract, oak extract, seaweed extract (comb, macombe, wakame, hijiki, hibamata, sujime Brown algae such as Tuna, Tuna, Tsuruarame, Chigaiso, Honda Rawa, Giant Kelp; Tengusa, Tsunomata, Sugiori, Usubanori, Asakusanori, Matsunori, Tosakamatsu, Funori, Ogonori, Kamensou, Igi Red algae such as Egonori; Chlorella, Aonori, Donariella, Chlorococcus, Anaaaosa, Kawanoori, Marimo, Shiogusa, Casanori, Futojuzumo, Tamajusmo, Aeguro, Aomidoro, etc .; Extract, grape extract, wheat extract, tomato extract, vitamin A and its derivatives (derivatives such as retinol and retinol palmitate, retinol acetate; derivatives such as retinal and dehydroretinal), carotenoids (carotene, lycopene, astaxanthin) Etc.), agarose, oligosaccharide, neo agarobiose, hydroquinone and its derivatives (arbutin, α-arbutin), cysteine and its derivatives, asparagus extract, acerola extract, seeker Kiss, Yuzu Extract, Sakura Extract, Ibuki Toranoo Extract, Neubara (Ages) Extract, Pea Extract, Greater Cranberry Extract, Chamomile Extract, Camcum Extract, Caquette Extract, Orange Extract, Raspberry Extract, Fire Thorn Extract, Kiwi extract, Clara extract, Japanese evening primrose extract, Coffee extract, Sesame oil, Sesame oil, Sesame extract, Sesame extract, Rice extract, Saisin extract, Hawthorn extract, Sunpens (Kawaketsumei) Extract, peony extract, white lily extract, mulberry extract, toki extract, beech extract, black currant extract, spinach extract, hop extract, mica (maika, hamanasu) extract, mokka ( Bokeh) extract, Yukinoshita extract, tea extract (Oolong tea) , Black tea, green tea, etc.), ganoderma extract, microbial fermentation metabolite, soybean extract, molasses extract, Rakan fruit extract and the like.

Among these whitening agents, particularly preferable ones exhibit a whitening effect by a mechanism different from that of phosphorylated saccharide calcium. Examples include vitamin C and its derivatives, α-arbutin and the like. That is, calcium phosphate phosphorylates alleviated pigmentation and skin dullness by smoothing out melanin excreted from melanocytes to epidermal cells by promoting the turnover of the epidermis, whereas vitamin C and its derivatives are Whitening effect is achieved by reducing the color of melanin. Further, α-arbutin exhibits a whitening effect by inhibiting the production of melanin by inhibiting the melanin synthase tyrosinase. Therefore, a synergistic whitening effect can be exhibited by combining phosphorylated sugar calcium and these whitening agents.

Examples of UV inhibitors include paramethoxycinnamate-2-ethylhexyl, 4-tert-butyl-4'-methoxydibenzoylmethane, oxybenzone and derivatives thereof (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4- Methoxybenzophenone-5-sulfonic acid, sodium 2-hydroxy-4-methoxybenzophenone-5-sulfonate, etc.), titanium oxide, fine particle titanium oxide, zinc oxide, barium sulfate and the like.

The skin external preparation composition combining the above ingredients and phosphorylated saccharide calcium is also prepared in various forms of bases known as normal skin external preparations, using the component phosphorylated saccharide calcium and the above ingredients, which are essential ingredients, in accordance with a conventional method. It can be prepared by blending.

The blending amount of the above components in the preparation of the external preparation for skin of the present invention varies depending on the kind of the medicinal agent, but is preferably in the range shown below. Within this range, when combined with phosphorylated saccharide calcium, there is no effect on the time-dependent stability of the phosphorylated saccharide calcium in the preparation, higher epidermal turnover promoting action, and epidermal keratinocyte differentiation promoting An action, an action of promoting the formation of tight junctions of the epidermis, an action of activating skin fibroblasts, and an action of promoting hyaluronic acid production from skin fibroblasts can be exhibited.

That is, the blending amount of the moisturizing agent in the skin external preparation composition of the present invention is preferably 0.00001 to 25%, more preferably 0.0001 to 20%. When the extract is used as an extract, it may be in this range as a dry solid content. If it is this range, the skin external preparation which shows the more excellent moisturizing effect and the favorable feeling of use without stickiness will be obtained.

Moreover, the compounding quantity of the antioxidant in the skin external preparation composition of this invention becomes like this. Preferably it is 0.00001-5%, More preferably, it is the range of 0.0001-3%. When the extract is used as an extract, it may be in this range as a dry solid content. If it is this range, the expression of the outstanding antioxidant effect will be seen and the skin external preparation which shows the outstanding anti-aging effect will be obtained.

Furthermore, the blending amount of the cell activator in the skin external preparation composition of the present invention is preferably 0.00001 to 5%, more preferably 0.0001 to 3%. When the extract is used as an extract, it may be in this range as the dry solid content. If it is this range, the skin roughening agent which the outstanding skin roughness improvement effect will express and the outstanding anti-aging effect is obtained.

Furthermore, the blending amount of the whitening agent in the skin external preparation composition of the present invention is preferably 0.00001 to 10%, more preferably 0.0001 to 5%. When the extract is used as an extract, it may be in this range as a dry solid content. If it is this range, the skin whitening agent which shows the more excellent whitening and skin beautification effect, and has a favorable feeling of use will be obtained.

Furthermore, as a compounding quantity of the ultraviolet inhibitor in the skin external preparation composition of this invention, Preferably it is 0.001 to 20%, More preferably, it is the range of 0.01 to 10%. If it is in this range, an excellent anti-ultraviolet effect is exhibited, and a skin external preparation exhibiting an excellent anti-aging effect can be obtained.

These moisturizers, antioxidants, cell activators, whitening agents and ultraviolet light inhibitors can be used singly or in combination.

In addition, the external preparation for skin of the present invention, as long as it does not impair the effects of the present invention, if necessary, usually other optional components used in preparations such as cosmetics, quasi drugs, and external medicines, , Water (including purified water, hot spring water, moisture obtained when distilling and extracting plants, etc.), oil agent, surfactant, metal soap, gelling agent, powder, alcohol, water-soluble polymer, film Forming agent, resin, clathrate compound, antibacterial agent, fragrance, deodorant, salt, pH adjuster, freshener, extract derived from plant / animal / microbe, blood circulation promoter, astringent, antiseborrheic agent, chelate Agents, keratolytic agents, enzymes, hormones, vitamins and the like can be added. Specific examples of suitable components include those shown below. Here, “derivatives” include salts that can be formed.

As an oil agent, it is a natural oil, a synthetic oil, or a solid oil as long as it is used for normal cosmetics as a constituent component or usability of the base, improving the feeling of use. Regardless of properties such as semi-solid or liquid, hydrocarbons, waxes, fatty acids, higher alcohols, ester oils, silicone oils, fluorine oils and the like can be used. Specific examples of this oil include hydrocarbons such as liquid paraffin, squalane, plant squalane, petrolatum, olive oil, castor oil, jojoba oil, mink oil, rice bran oil, macadamia nut oil, apricot oil, persic oil, safflower Oil, sunflower oil, avocado oil, medfoam oil, camellia oil, argan oil, almond oil, sesame oil, sesame oil, borage oil, cocoa butter, shea fat and other plant and animal oils, beeswax, honey beeswax, carnauba wax, Waxes such as candelilla wax and gay wax, stearic acid, isostearic acid, isostearyl isostearate, isocetyl myristate, octyl octanoate, dioctyl carbonate, macadamia nut fatty acid phytosteryl, long chain branched fatty acid cholesteryl, methyl polysiloxane, dimethyl polysilos Sun, decamethylcyclopentasiloxane, POE-modified silicone, a co-modified silicone, methyl phenyl polysiloxane.

Surfactants are used for emulsification and solubilization of oils and the like, and anionic, cationic, nonionic and amphoteric surfactants can be used. For example, POE sorbitan fatty acid esters such as POE hydrogenated castor oil, sucrose fatty acid ester, polyoxyethylene monolaurate (20), polyoxyethylene (20) sorbitan monostearate, polyoxyethylene (20) sorbitan monooleate, POE alkyl ethers such as polyoxyethylene (20) lauryl ether and polyoxyethylene (20) stearyl ether, sorbitan isostearic acid sorbitan, coconut oil fatty acid POE sorbitan, polyoxypropylene (10) methyl glucoside, glyceryl monostearate, isostearin Decaglyceryl acid, glyceryl trioctanoate, glyceryl POE triisostearate, glyceryl triisostearate, glyceryl tri-2-ethylhexanoate, hydrogenated Lecithin, N- sodium stearoyl glutamate, sodium surfactin include mannosylerythritol lipid like.

The metal soap is a metal salt other than an alkali salt such as a fatty acid, and examples thereof include aluminum stearate, magnesium stearate, and zinc laurate.

Gelling agents are used to improve the stability and usability of the system, and the feeling of use. Amino acid derivatives such as N-lauroyl-L-glutamic acid, dextrin fatty acid esters such as dextrin palmitate, sucrose fatty acid esters, Examples thereof include bentonite and organically modified clay minerals.

The powder is mainly used for various purposes such as coloring in makeup cosmetics, hiding skin, or improving the feeling of use, and if it is used in normal cosmetics, its shape (spherical, needle-like, plate , Etc.), particle diameter (smoke, fine particles, pigment grade, etc.) and particle structure (porous, non-porous, etc.) can be used. For example, as inorganic powder, barium sulfate, calcium carbonate, talc, mica, synthetic mica, mica, kaolin, sericite, silicic acid, anhydrous silicic acid, magnesium aluminum silicate, ceramic powder, boron nitride, silicon elastomer, spherical Examples of the organic powder include polyester powder, polyethylene powder, polystyrene powder, nylon powder, and lauroyl lysine. Examples of colored pigments include inorganic pigments such as iron oxide, carbon black, chromium oxide, bitumen, and ultramarine blue, those obtained by raked tar dyes, and those obtained by raketing natural dyes. Examples include titanium oxide-coated mica, titanium oxide-coated mica, bismuth oxychloride, bismuth oxychloride, titanium oxide-coated talc, fish scale foil, titanium oxide-coated colored mica, and other natural pigments such as tar pigments and carminic acid. . These powders may be combined, or surface treatment may be performed with an oil agent, silicone, or fluorine compound.

Examples of alcohols include lower alcohols such as ethanol and isopropanol, glycerin, diglycerin, ethylene glycol, diethylene glycol, triethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, and cetanol. .

The water-soluble polymer is used for stabilizing the system, improving the usability and the feeling of use, or obtaining a moisturizing effect. Specific examples of water-soluble polymers include plant polymers such as carrageenan, pectin, agar, locust bean gum, microbial polymers such as xanthan gum, animal polymers such as casein and gelatin, carboxymethyl glucan, starch and the like. Starch polymers, cellulose polymers such as methylcellulose, ethylcellulose, carboxymethylcellulose, hydroxypropylcellulose, nitrocellulose and crystalline cellulose, alginic acid polymers such as sodium alginate, vinyl polymers such as alkyl-modified carbomers and carboxyvinyl polymers , Polyoxyethylene polymer, polyoxyethylene polyoxypropylene copolymer polymer, acrylic polymer such as sodium polyacrylate, and the like. Also included are film forming agents such as polyvinyl alcohol and polyvinyl pyrrolidone.

As the plant extract or animal or microorganism-derived extract, those other than those mentioned as the above components (humectants, antioxidants, cell activators, whitening agents, and UV inhibitors) can be used.

Examples of the antibacterial agent include benzoic acid, sodium benzoate, paraoxybenzoic acid ester, parachlorometacresol, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol, chlorphenesin and the like.

Active oxygen scavengers are used for the purpose of inhibiting lipid peroxide production, and include superoxide dismutase, mannitol, quercetin, catechin and its derivatives, thiamines (thiamine hydrochloride, thiamine sulfate), riboflavins (riboflavin, riboflavin acetate) Etc.), vitamin Bs such as pyridoxines (such as pyridoxine hydrochloride and pyridoxine dioctanoate) and nicotinic acids (such as nicotinic acid amide and benzyl nicotinate); dibutylhydroxytoluene and butylhydroxyanisole.

Examples of the blood circulation promoter include arnica extract, red pepper tincture, ginkgo biloba extract, tocopherol acetate, γ-oryzanol, nicotinic acid, nicotinic acid derivatives such as tocopherol nicotinate, rutin, etc., and enzymes include lipase, papain, etc. Can be mentioned.

The skin external preparation of the present invention thus obtained can be in various forms without any particular restrictions. Examples of typical formulations include emulsions, creams, lotions, packs, cleansing agents, and makeup makeup. And cosmetics such as cosmetics, dispersions and ointments, and external medicines.

Next, although a reference example, a test example, and an Example are given and this invention is demonstrated further in detail, this invention is not restrict | limited at all to these. The phosphorylated saccharide calcium used in the following examples and reference examples was prepared from potato starch using calcium chloride instead of sodium chloride in the procedure of Example 1 of JP-A-8-104696. Point to. That is, phosphorylated saccharides in which 1 to 2 phosphate groups are bonded in the molecule to oligosaccharides composed of 2 to 8 glucoses linked with α-1,4, and calcium is bonded to each of these phosphorylated saccharides. It is a mixture of calcium. In this phosphorylated saccharide calcium, one phosphate group is bonded to an oligosaccharide consisting of 3, 4 or 5 glucose in the molecule, and calcium is bonded to this phosphate group. This is a mixture of an oligosaccharide composed of 7 or 8 glucoses with two phosphate groups bound in the molecule and calcium bound to the phosphate groups. Here, the molar ratio of the one having one phosphate group bonded to the one having two phosphate groups bonded is about 8: 2. In the following Examples and Test Examples, the salt thus prepared was used. In addition to this method using an ion exchange resin, phosphorylated saccharides of various metal salts can be easily prepared by adding each metal salt after desalting by general electrodialysis. In addition, about the calcium salt of phosphorylated saccharide, what is marketed as phosphorylated oligosaccharide calcium from Ezaki Glico Co., Ltd. can be used suitably. Phosphorylated oligosaccharide calcium is used as a powder or a solution.

Example 1 Promoting Hyaluronic Acid Production of Human Skin Fibroblasts Normal human skin fibroblasts NB1RGB were seeded in a 96-well microplate so that there were 2.5 × 10 ⁴ cells per well. The seeding medium was preincubated on a 96-well plate for 24 hours using a medium in which 10% by volume of fetal bovine serum (FBS) was added to Dulbecco's modified Eagle (D-MEM) medium. After removing the medium, the medium was replaced with a sample culture solution containing 0.5% phosphorylated oligosaccharide calcium prepared with 4% by volume FBS-added D-MEM and cultured at 37 ° C. under 5% CO ₂ for 48 hours. did. After completion of the culture, the amount of hyaluronic acid in the medium was measured using a hyaluronic acid measurement kit (manufactured by Seikagaku Biobusiness). These results are shown in Table 1.

As is clear from the results in Table 1, a significant hyaluronic acid production promoting effect was observed in the 0.5% phosphorylated oligosaccharide calcium addition group.

Example 2 Cell Proliferation Promoting Action of Human Skin Fibroblasts Normal human skin fibroblasts NB1RGB were seeded in a 96-well microplate so that there were 2.5 × 10 ⁴ cells per well. The seeding medium was pre-incubated in a 96-well plate at 37 ° C. in 5% CO _{2 for} 24 hours using a medium in which 10% by volume of fetal bovine serum (FBS) was added to Dulbecco's modified Eagle (D-MEM) medium. . After removing the medium, the sample culture solution containing various concentrations (0.2 to 0.6%) of phosphorylated oligosaccharide calcium prepared with 4% by volume FBS-added D-MEM was replaced with 37 ° C., 5% CO ₂ and cultured under. After 72 hours, cell growth was measured using Tetra Color One (Seikagaku Corporation). The growth rate of the control group was taken as 100, and the cell growth rate when adding phosphorylated oligosaccharide calcium was determined. These results are shown in Table 2.

As is clear from the results in Table 2, the hyaluronic acid production promoting effect was recognized in the 0.2 to 0.6% phosphorylated oligosaccharide calcium addition group.

Example 3 Human skin epidermal keratinocyte differentiation promoting action (involucrin protein production promoting effect)
Normal human dermal fibroblasts NHEK were seeded in a 12-well culture plate at 7.1 × 10 ⁴ per well. EpiLife KG-2 medium supplemented with insulin, hEGF, hydrocortisone, antibacterial agent, and BPE was used as the medium, and preincubation was performed at 37 ° C. in 5% CO _{2 for} 48 hours. After removing the medium, the medium was replaced with a sample culture solution containing 0.1% phosphorylated oligosaccharide calcium and cultured at 37 ° C. under 5% CO ₂ . After 144 hours, the cells were collected, and the expression level of involucrin as a differentiation marker was compared by Western blotting. Luminescence image analyzer LAS-4000UV
Detection with mini (FUJIFILM), the involucrin expression level in the control group was taken as 100, and the involucrin expression level when phosphorylated oligosaccharide calcium was added was determined. (GAPDH was used for internal standard correction.)
These results are shown in Table 3.

As is clear from the results in Table 3, a significant involucrin production promoting effect was observed in the 0.1% phosphorylated oligosaccharide calcium addition group. Since the expression level of involucrin, which is a differentiation marker, increased, it was confirmed that phosphorylated oligosaccharide calcium promotes differentiation of human skin epidermal keratinocytes.

Example 4 Human skin epidermal keratinocyte differentiation promoting action (involucrin gene expression promoting effect)
Normal human dermal fibroblasts NHEK were seeded in a 12-well culture plate at 7.1 × 10 ⁴ per well. EpiLife KG-2 medium supplemented with insulin, hEGF, hydrocortisone, antibacterial agent, and BPE was used as the medium, and preincubation was performed at 37 ° C. in 5% CO _{2 for} 48 hours. After removing the medium, the medium was replaced with a sample culture solution containing 0.1% phosphorylated oligosaccharide calcium and cultured at 37 ° C. under 5% CO ₂ . After 144 hours, total RNA was extracted from the cells, and the expression level of the involucrin gene was compared by real-time PCR.
The involucrin gene expression level in the control group was taken as 100, and the involucrin gene expression level when phosphorylated oligosaccharide calcium was added was determined. (GAPDH was used for internal standard correction.)
These results are shown in Table 4.

As is clear from the results in Table 4, the involucrin gene expression promoting effect was observed in the 0.1% phosphorylated oligosaccharide calcium addition group. Since the gene expression level of involucrin, which is a differentiation marker, increased, it was confirmed that phosphorylated oligosaccharide calcium promotes differentiation of human skin epidermal keratinocytes.

Example 5 Human skin epidermal keratinocytes promoted tight junction formation Normal human dermal fibroblasts NHEK were seeded in Transwell (manufactured by Millipore) at 4.3 × 10 ⁴ cells. Culture was performed for 4 days at 37 ° C. in 5% CO ₂ using EpiLife KG-2 medium supplemented with insulin, hEGF, hydrocortisone, antibacterial agent and BPE. After removing the medium, the medium was then replaced with a sample culture solution containing various concentrations of phosphorylated oligosaccharide calcium and cultured at 37 ° C. under 5% CO ₂ for 72 hours. Millicell-ERS epithelial voltohmmeter
TER (transepithelial over time in (Millipore)
Tight junction formation was evaluated by measuring electric resistance. These results are shown in FIG.
(Figure 1)

As is clear from the results of FIG. 1, it was revealed that the addition of phosphorylated oligosaccharide calcium to the medium promotes the formation of tight junctions of normal human epidermal keratinocytes in a concentration-dependent manner.

Example 6 Accelerating action of turnover of epidermis A lint cloth (2.Molecular solution of 0.35 ml of 10% by weight dihydroxyacetone (DHA) in 2 portions of each forearm of 4 healthy Japanese (3 men and 1 woman)). 5 × 2.5 cm) was applied for 8 to 10 hours to prepare a stratum corneum stained site. A 2% phosphorylated oligosaccharide calcium aqueous solution or a control solution was applied to the stained site twice a day in the morning and evening for about 2 weeks. The evaluation of turnover was performed by using a color difference meter (Minolta CR-400), and measuring each application site and adjacent sites 6 days and 12 days later. The recovery rate from the stratum corneum dyeing with DHA to before dyeing was evaluated by calculating the color difference. These results are shown in Table 5.

As is apparent from the results in Table 5, the color of the dyed portion returned quickly, that is, the stratum corneum turnover promoting action was shown in the 2% phosphorylated oligosaccharide calcium aqueous solution coating section.

The skin external preparations of various dosage forms containing the phosphorylated saccharide calcium of the present invention are exemplified below.
(Example 7) Lotion (component) (%)
(1) Glycerin

3.0
(2) 1,3-butylene glycol

2.0
(3) Propylene glycol

4.0
(4) Oleyl alcohol

0.1
(5) Polyoxyethylene monolaurate (20) sorbitan 1.5
(6) Polyoxyethylene (20) lauryl ether

0.5
(7) Olive oil

0.2
(8) Ethyl alcohol

20.0
(9) Methyl paraoxybenzoate

0.1
(10) dl-α-tocopherol acetate

0.2
(11) Dipotassium glycyrrhizinate

0.5
(12) Warmoko extract

0.5
(13) dl-Pyrrolidonecarboxylic acid

0.2
(14) Serine

0.2
(15) Sodium hyaluronate 1% solution

1.0
(16) Phosphorylated oligosaccharide calcium

10
(17) Purified water

Remaining amount

(Manufacturing method)
A. Components (1) to (10) are mixed and dissolved.
B. Components (11) to (17) are mixed and dissolved.
C. A lotion was obtained by uniformly mixing A and B.

(Example 8) Cream (ingredient) (%)
(1) Squalane

20.0
(2) Beeswax

5.0
(3) Jojoba oil

5.0
(4) Glyceryl monostearate

2.0
(5) Polyoxyethylene monostearate (20) Sorbitan 2.0
(6) Sorbitan monostearate
2.0
(7) Phytosterol

0.5
(8) Candelilla wax

1.0
(9) Glycerin

5.0
(10) Methyl paraoxybenzoate

0.1
(11) Hamamelis extract

1.0
(12) Mokutsu extract

1.0
(13) Carrot extract

1.0
(14) Ginkgo biloba extract

1.0
(15) Hydrolyzed rice extract

1.0
(16) Daylily extract

1.0
(17) Phosphorylated oligosaccharide calcium

1.0
(18) Purified water

Residual amount (19) Fragrance

Appropriate amount

(Manufacturing method)
A. Ingredients (1) to (10) are heated and mixed and maintained at 70 ° C.
B. Ingredient (18) is heat mixed and maintained at 70 ° C.
C. A and B are mixed, emulsified and cooled.
D. After cooling, components (11) to (17) and (19) were added to C to obtain a cream.

(Example 9) Emulsion (component) (%)
(1) Sorbitan monostearate

0.3
(2) Polyoxyethylene monooleate (20) sorbitan 0.1
(3) Lipophilic glyceryl monostearate

0.2
(4) Stearic acid

0.5
(5) Cetanol

0.5
(6) Squalane

3.0
(7) Liquid paraffin

4.0
(8) Grape seed oil

0.2
(9) Glyceryl tri-2-ethylhexanoate

2.0
(10) Dimethylpolysiloxane

1.0
(11) Hydrogenated soybean phospholipid

0.1
(12) dl-α-tocopherol nicotinate

0.1
(13) Ethyl paraoxybenzoate

0.1
(14) Carboxyvinyl polymer

0.1
(15) Sodium hydroxide

0.05
(16) Glycerin

5.0
(17) 1,3-butylene glycol

7.0
(18) Ethyl alcohol

5.0
(19) Phosphorylated oligosaccharide calcium
3.0
(20) Yeast extract

2.0
(21) Birch extract

2.0
(22) Maltose

0.2
(23) Purified water

Remaining amount (24) Fragrance

Appropriate amount

(Manufacturing method)
A. Components (14) to (17) and (23) are heated and mixed.
B. Components (1) to (13) are heated and mixed.
C. A and B are mixed and emulsified uniformly.
D. After cooling, components (18) to (22) and (24) were added to C to obtain an emulsion.

Example 7, Example 8 and Example 9 are skin lotions, creams, which have high skin safety, and are effective when applied to the skin to make the skin smooth and smooth, vivid and dull. It was an emulsion.

(Example 10) Pack (ingredient) (%)
(1) Polyvinyl alcohol

15.0
(2) Silicic anhydride

0.5
(3) Polyethylene glycol

0.5
(4) Macadamia nut oil

0.5
(5) Polyoxypropylene (10) methyl glucoside

5.0
(6) Glycerin

5.0
(7) Phosphorylated oligosaccharide calcium

0.5
(8) Tea extract

0.5
(9) Avocado extract

0.5
(10) Seaweed extract

0.5
(11) Ethyl alcohol

20.0
(12) Ethyl paraoxybenzoate

0.1
(13) Phosphate-L-ascorbyl magnesium

3.0
(14) Purified water

Remaining amount

(Manufacturing method)
A. Components (1) to (6) are mixed and heated to 70 ° C. to dissolve.
B. Components (7) to (14) are mixed and dissolved.
C. A and B were mixed and then cooled to obtain a pack.

Example 10 was a pack that was highly safe against the skin, and, as a result of application, moisturized and firmed the skin, and had an effect of making the skin dull.

(Example 11) Liquid foundation (component) (%)
(1) Liquid lanolin

2.0
(2) Liquid paraffin

5.0
(3) Stearic acid

2.0
(4) Cetanol

1.0
(5) Self-emulsifying glyceryl monostearate

1.0
(6) 2-methoxyhexyl paramethoxycinnamate

8.0
(7) 4-tert-butyl-4′-methoxydibenzoylmethane 2.0
(8) Retinol palmitate

0.2
(9) Methyl paraoxybenzoate

0.1
(10) Glycerin

5.0
(11) Triethanolamine

1.0
(12) Carboxymethyl cellulose

0.2
(13) Bentonite

0.5
(14) Titanium oxide

6.0
(15) Fine particle titanium oxide

2.0
(16) Fine zinc oxide

5.0
(17) Mica

2.0
(18) Talc

4.0
(19) Color pigment

4.0
(20) Phosphorylated oligosaccharide calcium

0.1
(21) Rosemary extract

0.5
(22) Ogon extract

0.5
(23) Purified water

Remaining amount (24) Fragrance

Appropriate amount

(Manufacturing method)
A. Components (1) to (9) are mixed and dissolved.
B. Add components (14) to (19) to A and mix uniformly.
C. Ingredients (10) to (13) and (23) are uniformly dissolved.
D. B and C are mixed and uniformly emulsified.
E. After cooling D, components (20) to (22) and (24) were added to obtain a liquid foundation.

(Example 12) Sunscreen emulsion (component) (%)
(1) Polyoxyalkylene-modified organopolysiloxane
1.0
(2) Dimethylpolysiloxane
5.0
(3) Octamethylcyclotetrasiloxane

20.0
(4) Isotridecyl isononanoate

5.0
(5) 2-methoxyhexyl paramethoxycinnamate

5.0
(6) Methyl paraoxybenzoate

0.1
(7) Silicone-treated fine particle titanium oxide

10.0
(8) Silicone-treated fine particle zinc oxide

10.0
(9) Polystyrene powder

3.0
(10) Trimethylsiloxysilicic acid

0.5
(11) Dipropylene glycol

3.0
(12) Ethyl alcohol

8.0
(13) Magnesium ascorbate phosphate

1.0
(14) α-Arbutin

1.0
(15) Phosphorylated oligosaccharide calcium

1.0
(16) Sodium chloride

0.2
(17) Warmoko extract

1.0
(18) Altea extract

1.0
(19) Honeysuckle extract

1.0
(20) Purified water

Remaining amount (21) Fragrance

Appropriate amount

(Manufacturing method)
A. Ingredients (1) to (10) are heated and mixed.
B. Ingredients (11) to (16) and (20) are warm-mixed.
C. A and B are mixed and uniformly emulsified.
D. After cooling, components (17) to (19) and (21) were added to C to obtain a sunscreen emulsion.

Examples 11 and 12 were liquid foundations and sunscreen emulsions that were highly safe to the skin and, as a result of application to the skin, prevented rough skin due to sunburn and the like, and had the effect of maintaining beautiful skin. In particular, Example 12 has a synergistic whitening effect because magnesium ascorbate phosphate, α-arbutin, and calcium phosphate phosphoric acid exert a whitening action by different mechanisms. That is, magnesium ascorbate promotes fading of melanin produced by sunburn. α-Arbutin inhibits melanin production by inhibiting tyrosinase, a melanin-producing enzyme. Phosphorylated sugar calcium also promotes the recovery of skin color after sunburn by promoting skin turnover. These actions confirmed a stronger whitening effect than using them alone.

(Formulation Example 13) Cream
component
(%)
(1) Stearic acid
1
(2) Glycerol monostearate
2
(3) Cetanol
2
(4) White beeswax
1
(5) Isocetyl myristate
1
(6) Decaglyceryl isostearate
1
(7) Ceramide 2
0.1
(8) Hydrogenated lecithin
0.1
(9) Plant squalane
5
(10) Macadamia nut oil
5
(11) Argan oil
0.1
(12) Kyonin oil
0.1
(13) Phosphorylated oligosaccharide calcium
1
(14) 1,3-butylene glycol
5
(15) Concentrated glycerin
5
(16) Sodium N-stearoyl glutamate
0.2
(17) Alkyl-modified carboxyvinyl polymer
0.05
(18) Oolong tea extract
0.1
(19) Brown algae extract
0.5
(20) Horsetail extract
0.1
(21) N-acetylglucosamine
0.1
(22) Watercress extract
0.1
(23) Sakura extract
0.05
(24) Avocado extract
0.2
(25) evening primrose extract
0.2
(26) Hydrolyzed silk
0.1
(27) Chlorphenesin
0.1
(28) Edetate
0.05
(29) Paraoxybenzoic acid ester
0.2
(30) Purified water
Remaining amount

(Manufacturing method)
A. Ingredients (1) to (12) are heated and mixed.
B. Ingredients (13)-(30) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain a cream.

(Prescription Example 14) Cream
component
(%)
(1) Stearic acid
1
(2) Glycerol monostearate
2
(3) Cetanol
2
(4) White beeswax
1
(5) Isocetyl myristate
1
(6) Decaglyceryl isostearate
1
(7) Ceramide 2
0.1
(8) Hydrogenated lecithin
0.1
(9) Plant squalane
5
(10) Macadamia nut oil
5
(11) Argan oil
0.1
(12) Kyonin oil
0.1
(13) Phosphorylated oligosaccharide calcium
2
(14) 1,3-butylene glycol
10
(15) Concentrated glycerin
5
(16) Sodium N-stearoyl glutamate
0.2
(17) Alkyl-modified carboxyvinyl polymer
0.05
(18) Oat extract
0.3
(19) Oolong tea extract
0.1
(20) Brown algae extract
0.5
(21) Horsetail extract
0.1
(22) N-acetylglucosamine
0.1
(23) Watercress extract
0.1
(24) Sakura extract
0.05
(25) Avocado extract
0.2
(26) Evening primrose extract
0.2
(27) Hydrolyzed silk
0.1
(28) Chlorphenesin
0.1
(29) Edetate
0.05
(30) Paraoxybenzoic acid ester
0.2
(31) Purified water
Remaining amount

(Manufacturing method)
A. Components (1) to (12) are heated and mixed.
B. Components (13) to (31) are heated and mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain a cream.

(Formulation Example 15) Cream
component
(%)
(1) Stearic acid
0.5
(2) Isostearic acid 0.5
(3) Glycerol monostearate
2
(4) Cetanol
2
(5) Shea fat
1
(6) Isocetyl myristate
1
(7) Decaglyceryl isostearate
1
(8) Ceramide 2
0.1
(9) Hydrogenated lecithin
0.1
(10) Plant squalane
5
(11) Macadamia nut oil
5
(12) Argan oil
0.1
(13) Kyonin oil
0.1
(14) Phosphorylated oligosaccharide calcium
1
(15) 1,3-butylene glycol
5
(16) Concentrated glycerin
5
(17) N-stearoyl sodium glutamate
0.2
(18) Alkyl-modified carboxyvinyl polymer
0.05
(19) Oat extract
0.1
(20) Oolong tea extract
0.1
(21) Brown algae extract
0.5
(22) Horsetail extract
0.1
(23) N-acetylglucosamine
0.1
(24) Watercress extract
0.1
(25) Sakura extract
0.05
(26) Avocado extract
0.2
(27) Evening primrose extract
0.2
(28) Hydrolyzed silk
0.1
(29) Chlorphenesin
0.1
(30) Edetate
0.05
(31) Paraoxybenzoic acid ester
0.2
(32) Purified water
Remaining amount

(Manufacturing method)
A. Components (1) to (13) are heated and mixed.
B. Components (14) to (32) are heated and mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain a cream.

(Formulation Example 16) Lotion
component
(%)
(1) Phosphorylated oligosaccharide calcium
1
(2) Decaglyceryl diisostearate
0.05
(3) POE hydrogenated castor oil (60E.O.)
0.5
(4) 1,3-butylene glycol
5
(5) White Willow Extract
0.2
(6) Phenoxyethanol
0.2
(7) Artichoke extract
0.05
(8) Hibiscus fermentation broth
0.1
(9) Dokudami extract
0.1
(10) Sodium citrate
0.05
(11) Diisopropylamine dichloroacetic acid
0.2
(12) Cedar extract
0.1
(13) Cam cam extract
0.1
(14) Jojoba leaf extract
0.1
(15) Water-soluble chlorophyll
0.02
(16) Avocado extract
0.2
(17) Souhakuhi extract
0.2
(18) Himalayan raspberry extract
0.2
(19) Sodium hyaluronate
0.001
(20) Hibiscus extract
0.2
(21) Fire thorn extract
0.1
(22) Houtus bean extract
0.5
(23) Chlorphenesin
0.05
(24) Fragrance
0.02
(25) Purified water
Remaining amount

(Manufacturing method)
A. Components (1) to (25) were heated and mixed, dissolved, and then cooled to obtain a lotion.

(Prescription Example 17) Lotion
component
(%)
(1) Phosphorylated oligosaccharide calcium
1
(2) Decaglyceryl diisostearate
0.05
(3) POE hydrogenated castor oil (60E.O.)
0.5
(4) 1,3-butylene glycol
5
(5) Dipropylene glycol
5
(6) White Willow Extract
0.2
(7) Ethanol
1
(8) Phenoxyethanol
0.2
(9) Artichoke extract
0.05
(10) Hibiscus fermentation broth
0.1
(11) Dokudami extract
0.1
(12) Xanthan gum
0.1
(13) Sodium citrate
0.05
(14) Diisopropylamine dichloroacetic acid
0.2
(15) Cedar extract
0.1
(16) Cam cam extract
0.1
(17) Jojoba leaf extract
0.1
(18) Water-soluble chlorophyll
0.05
(19) Avocado extract
0.2
(20) Souhaku extract
0.2
(21) Himalayan raspberry extract
0.2
(22) Sodium hyaluronate
0.001
(23) Hibiscus extract
0.5
(24) Fire thorn extract
0.3
(25) Houtus bean extract
0.5
(26) Chlorphenesin
0.05
(27) Fragrance
0.02
(28) Purified water
Remaining amount

(Manufacturing method)
A. Components (1) to (28) were heated and mixed, dissolved, and then cooled to obtain a lotion.

(Prescription Example 18) Lotion
component
(%)
(1) Phosphorylated oligosaccharide calcium
1
(2) Decaglyceryl diisostearate
0.05
(3) POE hydrogenated castor oil (60E.O.)
0.5
(4) 1,3-butylene glycol
5
(5) White Willow Extract
0.2
(6) Ethanol
5
(7) Phenoxyethanol
0.2
(8) Artichoke extract
0.05
(9) Hibiscus fermentation broth
0.1
(10) Dokudami extract
0.1
(11) Sodium citrate
0.05
(12) Carboxymethyl glucan solution
0.2
(13) Diisopropylamine dichloroacetic acid
0.2
(14) Carrageenan
0.1
(15) Cedar extract
0.1
(16) Cam cam extract
0.1
(17) Jojoba leaf extract
0.1
(18) Water-soluble chlorophyll
0.02
(19) Avocado extract
0.2
(20) Souhaku extract
0.2
(21) Himalayan raspberry extract
0.2
(22) Sodium hyaluronate
0.01
(23) Hibiscus extract
0.5
(24) Hauchiwa Bean Extract
0.5
(25) Chlorphenesin
0.05
(26) Fragrance
0.02
(27) Purified water
Remaining amount

(Manufacturing method)
A. Components (1) to (27) were heated and mixed, dissolved, and then cooled to obtain a lotion.

(Formulation example 19) Moisturizing gel
component
(%)
(1) Decamethylcyclopentasiloxane
10
(2) Isostearyl isostearate
1
(3) Jojoba oil
0.1
(4) Retinyl palmitate
0.1
(5) Dimethylpolysiloxane (6cs)
2
(6) Spherical silicon powder (Note 1)
1
(7) Tetraisopalmitate ascorbate
0.5
(8) Phosphorylated oligosaccharide calcium
1
(9) Moon peach leaf extract
0.5
(10) Aloe extract
0.1
(11) Bilberry extract
0.2
(12) Ascorbic acid glucoside
0.2
(13) 1,3 butylene glycol
5
(14) Sorbitol solution
3
(15) Polyethylene glycol 4000
1
(16) Carboxyvinyl polymer
0.2
(17) Orchid extract
0.1
(18) Water-soluble chlorophyll
0.02
(19) Bukuryu extract
0.1
(20) Potassium hydroxide
0.05
(21) Spruce extract
0.1
(22) Mevalonolactone
0.1
(23) Raffinose
1
(24) Paraoxybenzoic acid ester
0.2
(25) Potassium sorbate
0.02
(26) Edetate
0.02
(27) Purified water
Remaining amount Note 1: Tospearl manufactured by Momentive

(Manufacturing method)
A. Ingredients (1) to (7) are heated and mixed.
B. Ingredients (8)-(27) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain a moisturizing gel.

(Prescription Example 20) Moisturizing gel
component
(%)
(1) Decamethylcyclopentasiloxane
10
(2) Olive oil
1
(3) Retinyl palmitate
0.1
(4) Nicotinic acid dlα tocopherol
0.1
(5) Dimethylpolysiloxane (6cs)
2
(6) Spherical silicon powder (Note 1)
1
(7) Tetraisopalmitate ascorbate
0.5
(8) Phosphorylated oligosaccharide calcium
1
(9) Moon peach leaf extract
0.5
(10) Aloe extract
0.1
(11) Bilberry extract
0.2
(12) Ascorbic acid glucoside
0.2
(13) 1,3 butylene glycol
10
(14) Sorbitol solution
3
(15) Polyethylene glycol 4000
1
(16) Carboxyvinyl polymer
0.2
(17) Orchid extract
0.1
(18) Water-soluble chlorophyll
0.05
(19) Bukuryu extract
0.1
(20) Potassium hydroxide
0.05
(21) Spruce extract
0.1
(22) Mevalonolactone
0.1
(23) Raffinose
1
(24) Paraoxybenzoic acid ester
0.2
(25) Potassium sorbate
0.02
(26) Edetate
0.02
(27) Purified water
Remaining amount Note 1: Tospearl manufactured by Momentive

(Manufacturing method)
A. Ingredients (1) to (7) are heated and mixed.
B. Ingredients (8)-(27) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain a moisturizing gel.

(Formulation Example 21) Moisturizing gel
component
(%)
(1) Decamethylcyclopentasiloxane
10
(2) Macadamia nut oil
1
(3) Jojoba oil
0.1
(4) Dimethylpolysiloxane (6cs)
2
(5) Spherical silicon powder (Note 1)
5
(6) Ascorbic acid tetraisopalmitate
0.5
(7) Phosphorylated oligosaccharide calcium
1
(8) Moon peach leaf extract
0.5
(9) Aloe extract
0.1
(10) Bilberry extract
0.2
(11) Water-soluble licorice extract
0.1
(12) Ascorbic acid glucoside
0.2
(13) 1,3 butylene glycol
5
(14) Sorbitol solution
3
(15) Polyethylene glycol 4000
1
(16) Carboxyvinyl polymer
0.2
(17) Orchid extract
0.1
(18) Water-soluble chlorophyll
0.05
(19) Bukuryu extract
0.1
(20) Potassium hydroxide
0.05
(21) Spruce extract
0.1
(22) Mevalonolactone
0.1
(23) Raffinose
1
(24) Paraoxybenzoic acid ester
0.2
(25) Potassium sorbate
0.02
(26) Edetate
0.02
(27) Purified water
Remaining amount Note 1: Tospearl manufactured by Momentive

(Manufacturing method)
A. Ingredients (1) to (6) are heated and mixed.
B. Ingredients (8)-(27) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain a moisturizing gel.

(Prescription Example 22) Eye cream
component
(%)
(1) Co-modified silicon (Note 2)
2
(2) Decamethylcyclopentasiloxane
15
(3) Methyl polysiloxane
5
(4) Silicon elastomer dispersion (Note 4) 5
(5) Coenzyme Q10
0.03
(6) Oil-soluble licorice extract
0.01
(7) L-lysine hydrochloride
0.03
(8) Phosphorylated oligosaccharide calcium
0.02
(9) Pycnogenol
0.05
(10) Evening primrose extract
0.2
(11) Hibiscus extract
1
(12) Lactic acid bacteria culture solution
0.1
(13) Okra extract
0.1
(14) Pear juice fermented liquid
0.2
(15) Nicotinamide
0.1
(16) γ-amino-β-hydroxybutyric acid
0.2
(17) Prune extract 0.1
(18) Sodium chloride
1
(19) Dipropylene glycol
5
(20) Concentrated glycerin
5
(21) Raffinose
1
(22) Paraoxybenzoic acid ester
0.3
(23) N-methyl-L-serine
0.5
(24) Purified water
Remaining amount Note 2: ABIL manufactured by Evonik
EM90
Note 3: Silicon BY22-008 manufactured by Toray Dow Corning
Note 4: Toray Fill made by Toray Dow Corning Note 5: YOFCO made by Nippon Seika
CLE-NH

(Manufacturing method)
A. Ingredients (1) to (6) are heated and mixed.
B. Ingredients (7)-(24) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain an eye cream.

(Prescription Example 23) Eye cream
component
(%)
(1) Co-modified silicon (Note 2)
2
(2) POE-modified silicon dispersion (Note 3) 2
(3) Decamethylcyclopentasiloxane 20
(4) Methyl polysiloxane
2
(5) Silicon elastomer dispersion (Note 4)
2
(6) Coenzyme Q10
0.03
(7) Oil-soluble licorice extract
0.01
(8) L-lysine hydrochloride
0.03
(9) Phosphorylated oligosaccharide calcium
0.05
(10) Pycnogenol
0.05
(11) Evening primrose extract
0.2
(12) Hibiscus extract
1
(13) Lactic acid bacteria culture solution
0.1
(14) Okra extract
0.1
(15) Pear juice fermented liquid
0.2
(16) Nicotinamide
0.1
(17) γ-amino-β-hydroxybutyric acid 0.2
(18) Prune extract
0.1
(19) Sodium chloride
1
(20) Dipropylene glycol
5
(21) Concentrated glycerin
5
(22) Raffinose
1
(23) Paraoxybenzoic acid ester
0.3
(24) N-methyl-L-serine
0.5
(25) Purified water
Remaining amount Note 2: ABIL manufactured by Evonik
EM90
Note 3: Silicon BY22-008 manufactured by Toray Dow Corning
Note 4: Toray Fill made by Toray Dow Corning Note 5: YOFCO made by Nippon Seika
CLE-NH

(Manufacturing method)
A. Ingredients (1) to (7) are heated and mixed.
B. Ingredients (8)-(25) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain an eye cream.

(Prescription Example 24) Eye cream
component
(%)
(1) Co-modified silicon (Note 2)
2
(2) Squalane 10
(3) Decamethylcyclopentasiloxane 10
(4) Methylpolysiloxane 3
(5) Long-chain branched fatty acid cholesteryl (Note 5) 3
(6) Coenzyme Q10
0.03
(7) Oil-soluble licorice extract
0.01
(8) L-lysine hydrochloride
0.03
(9) Phosphorylated oligosaccharide calcium 0.05
(10) Pycnogenol 0.05
(11) Evening primrose extract
0.2
(12) Hibiscus extract 1
(13) Lactic acid bacteria culture solution 0.1
(14) Okra extract 0.1
(15) Pear juice fermented liquid
0.2
(16) Nicotinamide 0.1
(17) γ-amino-β-hydroxybutyric acid
0.2
(18) Prune extract
0.1
(19) Sodium chloride
1
(20) Dipropylene glycol
5
(21) Concentrated glycerin 5
(22) Raffinose 1
(23) Paraoxybenzoic acid ester 0.3
(24) N-methyl-L-serine
0.5
(25) Purified water
Remaining amount Note 2: ABIL manufactured by Evonik
EM90
Note 3: Silicon BY22-008 manufactured by Toray Dow Corning
Note 4: Toray Fill made by Toray Dow Corning Note 5: YOFCO made by Nippon Seika
CLE-NH

(Manufacturing method)
A. Ingredients (1) to (7) are heated and mixed.
B. Ingredients (8)-(25) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain an eye cream.

(Prescription Example 25) Sunscreen
component
(%)
(1) Dioctyl carbonate
22
(2) Co-modified silicon (Note 2)
2
(3) Titanium oxide
5
(4) Zinc oxide
5
(5) Coenzyme Q10
0.03
(6) Phosphorylated oligosaccharide calcium
1
(7) Crape myrtle extract
0.1
(8) White jellyfish polysaccharide
0.03
(9) γ-aminobutyric acid
0.1
(10) Ascorbic acid sulfate disodium salt
0.3
(11) Stevia extract
0.2
(12) Sugar ceramide (Note 6)
0.1
(13) Magnesium chloride
1
(14) 1,3 butylene glycol
5
(15) Phenoxyethanol
0.3
(16) Rice fermentation broth
1
(17) Licorice extract
0.1
(18) Oat extract
0.5
(19) Yeast extract (Note 7)
1
(20) Pearl protein extract
0.1
(21) Kyonin extract
0.1
(22) Dipotassium glycyrrhizinate
0.2
(23) White Willow Extract
0.2
(24) Sodium benzoate
0.03
(25) Purified water
Residual amount Note 2: ABIL EM90 manufactured by Evonik
Note 6: Bioceramide manufactured by Kibun Food Chemical Co., Ltd.
Note 7: Dismutin manufactured by Pentafarm

(Manufacturing method)
A. Ingredients (1) to (5) are heated and mixed.
B. Ingredients (6)-(25) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain a sunscreen.

(Formulation Example 26) Sunscreen
component
(%)
(1) Dioctyl carbonate
15
(2) Co-modified silicon (Note 2)
2
(3) Methylphenylpolysiloxane
3
(4) 2-Ethylhexyl paramethoxycinnamate
7
(5) Coenzyme Q10
0.03
(6) Phosphorylated oligosaccharide calcium
1
(7) Crape myrtle extract
0.1
(8) White jellyfish polysaccharide
0.03
(9) γ-aminobutyric acid
0.1
(10) Ascorbic acid sulfate disodium salt
0.3
(11) Stevia extract
0.2
(12) Sugar ceramide (Note 6)
0.1
(13) Magnesium chloride
1
(14) 1,3 butylene glycol
5
(15) Phenoxyethanol
0.3
(16) Rice fermentation broth
1
(17) Licorice extract
0.1
(18) Oat extract
0.5
(19) Yeast extract (Note 7)
1
(20) Pearl protein extract
0.1
(21) Kyonin extract
0.1
(22) Dipotassium glycyrrhizinate
0.2
(23) White Willow Extract
0.2
(24) Sodium benzoate
0.03
(25) Purified water
Residual amount Note 2: ABIL EM90 manufactured by Evonik
Note 6: Bioceramide manufactured by Kibun Food Chemical Co., Ltd.
Note 7: Dismutin manufactured by Pentafarm

(Manufacturing method)
A. Ingredients (1) to (5) are heated and mixed.
B. Ingredients (6)-(25) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain a sunscreen.

(Prescription Example 27) Sunscreen
component
(%)
(1) Dioctyl carbonate
10
(2) Co-modified silicon (Note 2)
2
(3) Glyceryl tri-2-ethylhexanoate
5
(4) Hardened oil
0.1
(5) Macadamia nut fatty acid phytosteryl
2
(6) 2-Ethylhexyl paramethoxycinnamate
7
(7) Titanium oxide
4
(8) Zinc oxide
4
(9) Coenzyme Q10
0.03
(10) Phosphorylated oligosaccharide calcium
1
(11) Great crape myrtle extract
0.1
(12) White jellyfish polysaccharide
0.03
(13) γ-aminobutyric acid
0.1
(14) Ascorbic acid sulfate disodium salt
0.3
(15) Stevia extract
0.2
(16) Sugar ceramide (Note 6)
0.1
(17) Magnesium chloride
1
(18) 1,3 butylene glycol
5
(19) Phenoxyethanol
0.3
(20) Rice fermentation broth
1
(21) Licorice extract
0.1
(22) Oat extract
0.5
(23) Yeast extract (Note 7)
1
(24) Pearl protein extract
0.1
(25) Kyonin extract
0.1
(26) Dipotassium glycyrrhizinate
0.2
(27) White Willow Extract
0.2
(28) Sodium benzoate
0.03
(29) Purified water
Residual amount Note 2: ABIL EM90 manufactured by Evonik
Note 6: Bioceramide manufactured by Kibun Food Chemical Co., Ltd.
Note 7: Dismutin manufactured by Pentafarm

(Manufacturing method)
A. Ingredients (1) to (9) are heated and mixed.
B. Ingredients (10)-(29) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain a sunscreen.

(Prescription Example 28) Cosmetic liquid
component
(%)
(1) Jojoba oil
0.5
(2) Olive oil (Note 9)
0.1
(3) Meadow foam oil
0.2
(4) Methylphenyl polysiooxane
0.5
(5) Retinol palmitate
0.05
(6) POE hydrogenated castor oil (80 EO)
1
(7) Olive squalane
0.2
(8) Phosphorylated oligosaccharide calcium
1
(9) Ethanol
7
(10) Concentrated glycerin
5
(11) Chlorphenesin
0.2
(12) Surfactin Na
0.1
(13) Alkyl-modified carbomer
0.3
(14) Hydrolyzed hyaluronic acid
0.1
(15) Lactic acid bacteria culture solution
0.2
(16) Dipropylene glycol
4
(17) Phenoxyethanol
0.3
(18) Polyethylene glycol 1000
1
(19) Gentian extract
0.1
(20) Ascorbic acid sulfate disodium salt
0.1
(21) Edetate
0.05
(22) Potassium hydroxide
0.02
(23) Yeast extract (Note 8)
0.03
(24) Cordyceps extract
0.05
(25) Peony extract
0.1
(26) Star fruit extract
0.1
(27) Ashitaba extract
0.1
(28) Seeker extract
0.3
(29) Tea extract
0.2
(30) Purified water
Residual amount Note 8: East Liquid ZB made by Ichimaru Falcos
Note 9: Cropure OL made by Croda Japan

(Manufacturing method)
A. Ingredients (1) to (7) are heated and mixed.
B. Ingredients (8)-(30) are warm mixed.
C. A and B were mixed and uniformly emulsified, and then cooled to obtain a cosmetic liquid.

(Prescription Example 28) Cleansing
component
(%)
(1) sorbitan isostearate
2
(2) Mannosyl erythritol lipid
0.1
(3) Rice bran oil
10
(4) Rice squalane
0.1
(5) POE glyceryl triisostearate
2
(6) Octyl octoate
2

(7) Phosphorylated oligosaccharide calcium
5
(8) Dipropylene glycol
2
(9) Ethanol
5
(10) Phenoxyethanol
0.2
(11) Paraoxybenzoic acid ester
0.3
(12) Ages extract
0.1
(13) Altea extract
0.2
(14) Palm oil fatty acid POE sorbitan
0.05
(15) Oil-soluble licorice extract
0.01
(16) Wheat germ extract
0.1
(17) Chlorphenesin
0.05
(18) Himefuuro extract
0.2
(19) Carboxyvinyl polymer
0.2
(20) Bilberry leaf extract
1
(21) Camellia extract
0.05
(22) Carboxymethylcellulose
0.02
(23) Ascorbic acid phosphate magnesium
0.01
(24) Yuzu extract
0.2
(25) Sorbitol solution
3
(26) Maple leaf extract
0.1
(27) Edetate
0.02
(28) Potassium hydroxide
0.05
(29) Yukinoshita extract
0.1
(30) Fragrance
0.02
(31) Purified water
Remaining amount

(Manufacturing method)
A. Ingredients (1) to (6) are heated and mixed.
B. Ingredients (7)-(31) are warm mixed.
C. A and B were mixed and uniformly emulsified and then cooled to obtain cleansing.

(Prescription Example 29) Cleansing
component
(%)
(1) sorbitan isostearate
2
(2) Mannosyl erythritol lipid
0.1
(3) Rice bran oil
5
(4) Rice squalane
0.1
(5) Glyceryl trioctanoate
5
(6) POE glyceryl triisostearate
2
(7) Octyl octoate
2
(8) Phosphorylated oligosaccharide calcium
5
(9) Dipropylene glycol
2
(10) Ethanol
5
(11) Phenoxyethanol
0.2
(12) Paraoxybenzoic acid ester
0.3
(13) Ages extract
0.1
(14) Altea extract
0.2
(15) Palm oil fatty acid POE sorbitan
0.05
(16) Oil-soluble licorice extract
0.01
(17) Wheat germ extract
0.1
(18) Chlorphenesin
0.05
(19) Himefuuro extract
0.2
(20) Carboxyvinyl polymer
0.2
(21) Bilberry leaf extract
1
(22) Camellia extract
0.05
(23) Carboxymethyl cellulose
0.02
(24) Ascorbic acid phosphate magnesium
0.01
(25) Yuzu extract
0.2
(26) Sorbitol solution
3
(27) Maple leaf extract
0.1
(28) Edetate
0.02
(29) Potassium hydroxide
0.05
(30) Yukinoshita extract
0.1
(31) Fragrance
0.02
(32) Purified water
Remaining amount

(Manufacturing method)
A. Ingredients (1) to (7) are heated and mixed.
B. Ingredients (8)-(32) are warm mixed.
C. A and B were mixed and uniformly emulsified and then cooled to obtain cleansing.

(Formulation example 30) Massage cream
component
(%)
(1) sorbitan isostearate
0.1
(2) Medfoam oil
1
(3) Olive oil (Note 9)
5
(4) Avocado oil
0.5
(5) Liquid paraffin
5
(6) Triisostearic glyceride
0.1
(7) Ascorbyl tetraisopalmitate
0.1
(8) POE hydrogenated castor oil (40E.O.)
1
(9) Phosphorylated oligosaccharide calcium
1
(10) Chimp extract
0.2
(11) Dipropylene glycol
2
(12) Raffinose
0.5
(13) Ethanol
5
(14) Phenoxyethanol
0.2
(15) Bentonite
0.1
(16) Giou extract
0.1
(17) Crape myrtle extract
0.1
(18) N-methyl-L-serine
0.5
(19) Xanthan gum
0.2
(20) Toki extract
0.1
(21) Senkyu extract
0.1
(22) Ogon Extract
0.3
(23) Barley extract
0.2
(24) Oil-soluble licorice extract
0.01
(25) Carboxyvinyl polymer
0.3
(26) Chlorphenesin
0.05
(27) Ascorbic acid phosphate magnesium
0.01
(28) Sorbitol solution
3
(29) Edetate
0.02
(30) Potassium hydroxide
0.07
(31) Fragrance
0.02
(32) Purified water
Remaining amount Note 9: Cropure OL made by Croda Japan

(Manufacturing method)
A. Ingredients (1) to (8) are heated and mixed.
B. Ingredients (9) to (32) are heated and mixed.
C. A was added to B and mixed, and the mixture was uniformly emulsified to obtain a massage cream.

Claims (5)

A skin external preparation characterized by containing phosphorylated saccharide calcium as a turnover promoting component. A skin external preparation characterized by containing phosphorylated saccharide calcium as a keratinocyte differentiation promoting component. A skin external preparation characterized by containing phosphorylated saccharide calcium as a tight junction formation promoting component. A skin external preparation characterized by containing phosphorylated saccharide calcium as a cell activation component. A skin external preparation characterized by containing phosphorylated saccharide calcium as a hyaluronic acid production promoting component.