JP5888753B2 - 効率的な人工多能性幹細胞の樹立方法 - Google Patents

効率的な人工多能性幹細胞の樹立方法 Download PDF

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JP5888753B2
JP5888753B2 JP2013512280A JP2013512280A JP5888753B2 JP 5888753 B2 JP5888753 B2 JP 5888753B2 JP 2013512280 A JP2013512280 A JP 2013512280A JP 2013512280 A JP2013512280 A JP 2013512280A JP 5888753 B2 JP5888753 B2 JP 5888753B2
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cyclin
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JP2013544069A (ja
JP2013544069A5 (cg-RX-API-DMAC7.html
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伸弥 山中
伸弥 山中
和利 高橋
和利 高橋
剛士 田邊
剛士 田邊
ヒョンジョン ホン
ヒョンジョン ホン
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Kyoto University NUC
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JP2013512280A 2010-11-04 2011-11-04 効率的な人工多能性幹細胞の樹立方法 Active JP5888753B2 (ja)

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US41017810P 2010-11-04 2010-11-04
US61/410,178 2010-11-04
PCT/JP2011/076017 WO2012060473A1 (en) 2010-11-04 2011-11-04 Method of efficiently establishing induced pluripotent stem cells

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JP2013544069A JP2013544069A (ja) 2013-12-12
JP2013544069A5 JP2013544069A5 (cg-RX-API-DMAC7.html) 2014-12-18
JP5888753B2 true JP5888753B2 (ja) 2016-03-22

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EP (1) EP2635678A4 (cg-RX-API-DMAC7.html)
JP (1) JP5888753B2 (cg-RX-API-DMAC7.html)
WO (1) WO2012060473A1 (cg-RX-API-DMAC7.html)

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Publication number Priority date Publication date Assignee Title
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
KR20250035015A (ko) 2011-12-05 2025-03-11 팩터 바이오사이언스 인크. 세포를 형질감염시키는 방법들 및 생성물들
BR122019025681B1 (pt) 2012-11-01 2023-04-18 Factor Bioscience Inc Método para inserir uma sequência de ácido nucleico em uma localização segura de um genoma de uma célula
MX372651B (es) 2014-01-31 2020-06-16 Factor Bioscience Inc ARN SINTÉTICO PARA SU Uso EN EL TRATAMIENTO DE LA EPIDERMÓLISIS AMPOLLOSA DISTRÓFICA.
JP7199809B2 (ja) 2015-02-13 2023-01-06 ファクター バイオサイエンス インコーポレイテッド 核酸製品及びその投与方法
JP7563872B2 (ja) 2016-05-25 2024-10-08 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 癌を処置するための方法及び組成物
EP3500585A4 (en) 2016-08-17 2020-04-01 Factor Bioscience Inc. NUCLEIC ACID PRODUCTS AND METHODS OF ADMINISTRATION
US11679148B2 (en) 2017-11-24 2023-06-20 Institut National De La Santé Et De La Recherche Médicale (Inserm) Methods and compositions for treating cancers
US20210236633A1 (en) 2018-08-06 2021-08-05 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and compositions for treating cancers
WO2020230138A1 (en) 2019-05-14 2020-11-19 Aleph Farms Ltd. Pluripotent cell aggregates and use thereof
CN114450265B (zh) 2019-07-03 2024-12-24 菲克特生物科学股份有限公司 阳离子脂质及其用途
US10501404B1 (en) 2019-07-30 2019-12-10 Factor Bioscience Inc. Cationic lipids and transfection methods
JP2023550181A (ja) 2020-11-24 2023-11-30 モナシュ ユニバーシティ 方法及び細胞構造体
KR20230113768A (ko) 2020-11-24 2023-08-01 모나쉬 유니버시티 유도 줄기 세포
WO2022207889A1 (en) 2021-04-01 2022-10-06 INSERM (Institut National de la Santé et de la Recherche Médicale) Liver organoid manufacturing methods, liver organoids obtained with the same, and uses thereof

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AUPQ307399A0 (en) * 1999-09-24 1999-10-21 Luminis Pty Limited Cell cycle control
US9567591B2 (en) * 2003-05-15 2017-02-14 Mello Biotechnology, Inc. Generation of human embryonic stem-like cells using intronic RNA
AU2006325975B2 (en) 2005-12-13 2011-12-08 Kyoto University Nuclear reprogramming factor
CA2683056C (en) 2007-04-07 2020-03-24 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
CA2660123C (en) 2007-10-31 2017-05-09 Kyoto University Nuclear reprogramming method
CN101970664B (zh) 2008-01-16 2013-08-21 林希龙 使用可诱导的重组核糖核酸因子生成不含肿瘤的类胚胎干细胞的多能性细胞
JP2011525794A (ja) * 2008-06-26 2011-09-29 国立大学法人大阪大学 iPS細胞の製造方法および製造キット
CA2695590C (en) * 2008-06-27 2018-01-09 Kyoto University Method of efficiently establishing induced pluripotent stem cells
EP2295538B1 (en) * 2008-07-07 2015-10-21 Takara Bio Inc. Method for production of pluripotent stem cell

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EP2635678A4 (en) 2014-04-23
US20130183757A1 (en) 2013-07-18
JP2013544069A (ja) 2013-12-12
US9637732B2 (en) 2017-05-02
EP2635678A1 (en) 2013-09-11
WO2012060473A1 (en) 2012-05-10

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