JP5883265B2 - ヒト肝細胞を担持するキメラ非ヒト動物 - Google Patents

ヒト肝細胞を担持するキメラ非ヒト動物 Download PDF

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Publication number
JP5883265B2
JP5883265B2 JP2011226233A JP2011226233A JP5883265B2 JP 5883265 B2 JP5883265 B2 JP 5883265B2 JP 2011226233 A JP2011226233 A JP 2011226233A JP 2011226233 A JP2011226233 A JP 2011226233A JP 5883265 B2 JP5883265 B2 JP 5883265B2
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human
human animal
cyp3a
mice
scid
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Expired - Fee Related
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JP2011226233A
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English (en)
Japanese (ja)
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JP2013085485A5 (enExample
JP2013085485A (ja
Inventor
光雄 押村
光雄 押村
康宏 香月
康宏 香月
知世 向谷
知世 向谷
島田　卓
卓 島田
雅和 加国
雅和 加国
理子 浜村
理子 浜村
秀隆 神村
秀隆 神村
章生 河村
章生 河村
直之 中田
直之 中田
雅人 大渕
雅人 大渕
弘太 加藤
弘太 加藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tottori University NUC
Phoenixbio Co Ltd
Original Assignee
Tottori University NUC
Phoenixbio Co Ltd
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Publication date
Application filed by Tottori University NUC, Phoenixbio Co Ltd filed Critical Tottori University NUC
Priority to JP2011226233A priority Critical patent/JP5883265B2/ja
Priority to EP12840420.9A priority patent/EP2767160B1/en
Priority to CA2851997A priority patent/CA2851997C/en
Priority to PCT/JP2012/077019 priority patent/WO2013054949A1/ja
Priority to US14/351,481 priority patent/US9420769B2/en
Publication of JP2013085485A publication Critical patent/JP2013085485A/ja
Publication of JP2013085485A5 publication Critical patent/JP2013085485A5/ja
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/12—Animals modified by administration of exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Organic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2011226233A 2011-10-13 2011-10-13 ヒト肝細胞を担持するキメラ非ヒト動物 Expired - Fee Related JP5883265B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2011226233A JP5883265B2 (ja) 2011-10-13 2011-10-13 ヒト肝細胞を担持するキメラ非ヒト動物
EP12840420.9A EP2767160B1 (en) 2011-10-13 2012-10-12 Chimeric non-human animal carrying human hepatocyte
CA2851997A CA2851997C (en) 2011-10-13 2012-10-12 Chimeric non-human animal carrying human hepatocyte
PCT/JP2012/077019 WO2013054949A1 (ja) 2011-10-13 2012-10-12 ヒト肝細胞を担持するキメラ非ヒト動物
US14/351,481 US9420769B2 (en) 2011-10-13 2012-10-12 Chimeric non-human animal carrying human hepatocyte

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2011226233A JP5883265B2 (ja) 2011-10-13 2011-10-13 ヒト肝細胞を担持するキメラ非ヒト動物

Publications (3)

Publication Number Publication Date
JP2013085485A JP2013085485A (ja) 2013-05-13
JP2013085485A5 JP2013085485A5 (enExample) 2014-10-30
JP5883265B2 true JP5883265B2 (ja) 2016-03-09

Family

ID=48081988

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JP2011226233A Expired - Fee Related JP5883265B2 (ja) 2011-10-13 2011-10-13 ヒト肝細胞を担持するキメラ非ヒト動物

Country Status (5)

Country Link
US (1) US9420769B2 (enExample)
EP (1) EP2767160B1 (enExample)
JP (1) JP5883265B2 (enExample)
CA (1) CA2851997C (enExample)
WO (1) WO2013054949A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6496107B2 (ja) 2014-05-08 2019-04-03 株式会社フェニックスバイオ 高尿酸血症モデル
CN109688810B (zh) 2016-06-27 2022-06-03 贝勒医学院 具有缺陷性p450氧化还原酶的人肝嵌合非人动物及其使用方法
CA3143640A1 (en) * 2019-07-26 2021-02-04 Fei Yi Methods and compositions for producing hepatocytes
WO2021132117A1 (ja) * 2019-12-23 2021-07-01 積水メディカル株式会社 ヒト肝細胞キメラ動物におけるヒト肝細胞置換率の測定方法
JP2024533772A (ja) * 2021-09-30 2024-09-12 ユニバーシティ オブ サザン カリフォルニア ヒト肝細胞培養培地およびｉｎ ｖｉｔｒｏ培養ヒト肝細胞の馴化培地ならびにその使用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3266766B2 (ja) 1994-08-23 2002-03-18 科学技術振興事業団 ローン性増殖能を有する肝実質細胞とその取得方法、並びにその継代培養方法
JP3211941B2 (ja) 1996-12-26 2001-09-25 科学技術振興事業団 ヒト小型肝細胞の取得方法と、この細胞の初代培養および継代培養方法
AU6476000A (en) * 1999-08-13 2001-03-13 Kirin Beer Kabushiki Kaisha Mouse having human cytochrome p450 transferred therein
US6995299B2 (en) * 1999-11-02 2006-02-07 University Of Connecticut Propagation of human hepatocytes in non-human animals
US6509514B1 (en) * 2000-03-17 2003-01-21 Kmt Hepatech, Inc. Chimeric animal model susceptible to human hepatitis C virus infection
JP2002045087A (ja) 2000-05-19 2002-02-12 Japan Science & Technology Corp キメラ動物
JP4334358B2 (ja) * 2002-03-25 2009-09-30 財団法人 ひろしま産業振興機構 増殖性ヒト肝細胞を認識する抗体と、増殖性ヒト肝細胞および機能性ヒト肝細胞
WO2008001614A1 (fr) 2006-06-29 2008-01-03 Hiroshima Industrial Promotion Organization Modèle animalier de stéatohépatite non alcoolique et modèle animalier de stéatose hépatique
GB0720552D0 (en) * 2007-10-19 2007-11-28 Iti Scotland Ltd Multiple cluster knockout
WO2009063722A1 (ja) * 2007-11-14 2009-05-22 National University Corporation Tottori University ヒトチトクロームp450遺伝子（クラスター）を含む哺乳動物人工染色体ベクター及びそれを保持する非ヒト哺乳動物
JP5557217B2 (ja) * 2010-01-06 2014-07-23 国立大学法人鳥取大学 マウス人工染色体ベクター
KR20110116817A (ko) 2010-04-20 2011-10-26 삼성전기주식회사 열전 모듈을 이용한 건축용 단열재

Also Published As

Publication number Publication date
US9420769B2 (en) 2016-08-23
CA2851997C (en) 2018-07-10
EP2767160A1 (en) 2014-08-20
CA2851997A1 (en) 2013-04-18
JP2013085485A (ja) 2013-05-13
US20140241991A1 (en) 2014-08-28
EP2767160A4 (en) 2015-07-01
WO2013054949A1 (ja) 2013-04-18
EP2767160B1 (en) 2017-01-18

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