JP5881206B2 - 骨再生材料 - Google Patents
骨再生材料 Download PDFInfo
- Publication number
- JP5881206B2 JP5881206B2 JP2011251789A JP2011251789A JP5881206B2 JP 5881206 B2 JP5881206 B2 JP 5881206B2 JP 2011251789 A JP2011251789 A JP 2011251789A JP 2011251789 A JP2011251789 A JP 2011251789A JP 5881206 B2 JP5881206 B2 JP 5881206B2
- Authority
- JP
- Japan
- Prior art keywords
- ocp
- bone
- nano
- gelatin
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000463 material Substances 0.000 title claims description 26
- 230000010478 bone regeneration Effects 0.000 title claims description 24
- 108010010803 Gelatin Proteins 0.000 claims description 32
- 229920000159 gelatin Polymers 0.000 claims description 32
- 235000019322 gelatine Nutrition 0.000 claims description 32
- 235000011852 gelatine desserts Nutrition 0.000 claims description 32
- 239000008273 gelatin Substances 0.000 claims description 31
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 14
- 239000001506 calcium phosphate Substances 0.000 claims description 11
- 235000011010 calcium phosphates Nutrition 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 10
- 239000011148 porous material Substances 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 8
- 239000006185 dispersion Substances 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 210000000988 bone and bone Anatomy 0.000 description 47
- 239000000499 gel Substances 0.000 description 29
- 230000007547 defect Effects 0.000 description 17
- 102000008186 Collagen Human genes 0.000 description 11
- 108010035532 Collagen Proteins 0.000 description 11
- 229920001436 collagen Polymers 0.000 description 11
- 239000008187 granular material Substances 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- 241000700159 Rattus Species 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000007864 aqueous solution Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 210000003625 skull Anatomy 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- 238000001356 surgical procedure Methods 0.000 description 5
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 4
- 238000004132 cross linking Methods 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 239000000919 ceramic Substances 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 208000006735 Periostitis Diseases 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000004064 dysfunction Effects 0.000 description 2
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 230000000399 orthopedic effect Effects 0.000 description 2
- 210000003460 periosteum Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001172 regenerating effect Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 238000001878 scanning electron micrograph Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 208000006386 Bone Resorption Diseases 0.000 description 1
- 208000020084 Bone disease Diseases 0.000 description 1
- 208000018084 Bone neoplasm Diseases 0.000 description 1
- 229910014497 Ca10(PO4)6(OH)2 Inorganic materials 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010009260 Cleft lip and palate Diseases 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108010059712 Pronase Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000024279 bone resorption Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- VSGNNIFQASZAOI-UHFFFAOYSA-L calcium acetate Chemical compound [Ca+2].CC([O-])=O.CC([O-])=O VSGNNIFQASZAOI-UHFFFAOYSA-L 0.000 description 1
- 239000001639 calcium acetate Substances 0.000 description 1
- 229960005147 calcium acetate Drugs 0.000 description 1
- 235000011092 calcium acetate Nutrition 0.000 description 1
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000004720 cerebrum Anatomy 0.000 description 1
- 208000016653 cleft lip/palate Diseases 0.000 description 1
- 238000012669 compression test Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 210000001951 dura mater Anatomy 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002695 general anesthesia Methods 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000010299 mechanically pulverizing process Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 229940105631 nembutal Drugs 0.000 description 1
- 230000011164 ossification Effects 0.000 description 1
- 230000004072 osteoblast differentiation Effects 0.000 description 1
- 210000002997 osteoclast Anatomy 0.000 description 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
- WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 208000028169 periodontal disease Diseases 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000004626 polylactic acid Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 230000009772 tissue formation Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- -1 β-TCP) Chemical class 0.000 description 1
Images
Landscapes
- Materials For Medical Uses (AREA)
Description
(Nano−OCP/Gelディスクの調製)
OCPを非特許文献1に記載の方法により調製した。OCPの沈殿物を乳鉢で粉砕し、粉砕物を篩により100〜300μmの顆粒に整粒した。整粒したOCP顆粒100mgを、硬組織破砕装置(安井器械株式会社製、マルチビーズショッカー)を用いて2500rpm×30秒×1回の条件にて機械的に粉砕した。容器内壁に付着した粉末を乳鉢で粉砕した。このようにして、Nano−OCPを調製した。
(OCP/Gelディスクの調製)
ゼラチン(シグマ社製、Type A Gelatin)をリン酸緩衝液(リン酸2水素ナトリウム)に溶解して、1%(w/v)のゼラチンを含む水溶液を調製した。このゼラチン溶液500mLを攪拌しながら70℃に維持した。この溶液に、0.08モル/Lの酢酸カルシウム水溶液500mLを7.5分間かけて滴下して混合した。この混合液をさらに70℃にて数分間静置することにより、OCP/ゼラチン(OCP/Gel)の沈殿(共沈物)を形成させた。上清を除去し、沈殿の懸濁液を内径9mmの遠沈用樹脂製チューブに充填し、−25℃にて一晩維持して凍結した。凍結した懸濁液を−45℃にて24時間凍結乾燥した。凍結乾燥物を133Pa以下の減圧下、150℃にて24時間維持し、熱架橋した。チューブの先端を切断し、凍結乾燥物を一定体積でチューブ外へ押し出し、ミクロトームナイフを用いて1mm厚でカッティングしてOCP/Gel(40/60)ディスクを成形した。
(細孔径)
実施例1で得られた各ディスクの細孔径を水銀圧入式細孔分布測定装置(マルバーン社製、PoreMaster 60 GT)にて解析した。結果を図2に示す。
実施例1および比較例1で得られた各ディスクの弾性率を万能試験機(株式会社島津製作所製、EZ−L−500N)により測定した。内径9mm×厚み1mmのディスクを圧縮試験用治具に設置し、一定のクロスヘッドスピードで荷重−変位曲線を測定して、連続性のある曲線部分にて、荷重−変位の値から応力−ひずみの値(応力/ひずみ:弾性率)を計算した。結果を図3に示す。
ジエチルエーテルおよびネンブタールを用いて、ラット(Wistar、12週齢、雄性)に全身麻酔を施した。次いで、ラットの頭蓋部皮膚を剃毛し、露出した皮膚およびその下の骨膜をメスで切開した。頭蓋骨正中部に直径9mmの骨欠損部を形成した。次いで、実施例1で得られたNano−OCP/Gel(90/10)ディスク、Nano−OCP/Gel(50/50)ディスクおよび比較例1で得られたOCP/Gel(40/60)を電子線照射(5kGy)により滅菌し、骨欠損部に埋入して骨膜および皮膚を縫合した。
Claims (3)
- 粒子径が5〜1000nmの第8リン酸カルシウム微粉末とゼラチンとの複合体を含み、10〜500μmの細孔を有する骨再生材料。
- 前記微粉末が、前記ゼラチン1質量部に対して1〜9質量部である、請求項1に記載の骨再生材料。
- 骨再生材料の製造方法であって、粒子径が5〜1000nmの第8リン酸カルシウム微粉末をゼラチン水溶液に分散させた分散液を凍結乾燥する工程を含む、方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2011251789A JP5881206B2 (ja) | 2011-11-17 | 2011-11-17 | 骨再生材料 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2011251789A JP5881206B2 (ja) | 2011-11-17 | 2011-11-17 | 骨再生材料 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2013106644A JP2013106644A (ja) | 2013-06-06 |
JP5881206B2 true JP5881206B2 (ja) | 2016-03-09 |
Family
ID=48704066
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011251789A Active JP5881206B2 (ja) | 2011-11-17 | 2011-11-17 | 骨再生材料 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP5881206B2 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020071497A1 (ja) | 2018-10-04 | 2020-04-09 | ニプロ株式会社 | 骨再生材料 |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP6265665B2 (ja) * | 2013-09-11 | 2018-01-24 | 国立大学法人東北大学 | 骨接合部再生材料 |
US10064979B2 (en) | 2013-12-02 | 2018-09-04 | Tohoku University | Bone regeneration material |
JP6918326B2 (ja) * | 2017-10-19 | 2021-08-11 | 国立研究開発法人物質・材料研究機構 | デキサメタゾンを含有する複合多孔質足場材料およびその製造方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2544075B2 (ja) * | 1993-04-12 | 1996-10-16 | 新田ゼラチン株式会社 | 医科歯科用硬化体の製造方法 |
JP5046511B2 (ja) * | 2004-11-19 | 2012-10-10 | 日本ハム株式会社 | 硬組織代替性担体材料 |
ITMI20071298A1 (it) * | 2007-06-29 | 2008-12-30 | Univ Bologna Alma Mater | Materiale composito poroso, relativo processo di preparazione e suo uso per la realizzazione di dispositivi per l'ingegneria tissutale |
JP5647432B2 (ja) * | 2010-05-06 | 2014-12-24 | ニプロ株式会社 | 骨再生材料 |
-
2011
- 2011-11-17 JP JP2011251789A patent/JP5881206B2/ja active Active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020071497A1 (ja) | 2018-10-04 | 2020-04-09 | ニプロ株式会社 | 骨再生材料 |
Also Published As
Publication number | Publication date |
---|---|
JP2013106644A (ja) | 2013-06-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5046511B2 (ja) | 硬組織代替性担体材料 | |
Fellah et al. | Bone repair using a new injectable self‐crosslinkable bone substitute | |
JP2008513100A (ja) | 多孔度生体材料−充填剤複合体およびその製法 | |
JP5647432B2 (ja) | 骨再生材料 | |
JP5881206B2 (ja) | 骨再生材料 | |
EP2453932A1 (en) | Polymeric collagen biomaterials | |
Hu et al. | Sr-HA scaffolds fabricated by SPS technology promote the repair of segmental bone defects | |
US10517993B2 (en) | Porous composite, bone regeneration material, and method for producing porous composite | |
Chen et al. | Reconstruction of calvarial defect using a tricalcium phosphate-oligomeric proanthocyanidins cross-linked gelatin composite | |
TWI687222B (zh) | 多孔質複合體、及含有磷酸八鈣及副甲狀腺素的多孔質複合體的製造方法 | |
WO2009009520A2 (en) | Nanoparticulate fillers | |
JP2006034485A (ja) | 生体吸収性顆粒状多孔質骨補填材及びその製造方法 | |
ES2357191T3 (es) | Material compuesto poroso, correspondiente proceso de preparación y su uso para realizar dispositivo para ingeniería tisular. | |
JP7412700B2 (ja) | 多孔質複合体 | |
EP2200670B1 (fr) | Substitut osseux comprenant un agent de contraste, son procede de preparation et ses utilisations | |
US20180078676A1 (en) | Porous composite, bone regeneration material, and method for producing porous composite | |
Asano et al. | Preparation of thermoplastic poly (L-lactic acid) membranes for guided bone regeneration. | |
US10512709B2 (en) | Porous composite and bone regeneration material | |
LT6309B (lt) | Trimatis porėtas celiuliozės karkasas kaulo inžinerijai ir jo gavimo būdas | |
JPWO2015083668A1 (ja) | 骨再生材料 | |
WO2023090355A1 (ja) | 血管新生材料、骨再生促進材料、血管新生材料の製造方法及び骨再生促進材料の製造方法 | |
WO2024189842A1 (ja) | 歯根膜再生材料 | |
Shubhangini et al. | A Novel Candidate for Guided Tissue Regeneration: Chitosan and Eggshell Membrane | |
Chatterjee et al. | A Novel Candidate for Guided Tissue Regeneration: Chitosan and Eggshell Membrane | |
WO2023095781A1 (ja) | 骨再生促進材料、骨再生促進材料の製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20140724 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20140724 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150630 |
|
RD03 | Notification of appointment of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7423 Effective date: 20150717 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20150828 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20150828 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20160112 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20160129 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5881206 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: R3D04 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |