JP5827948B2 - ウイルスを単離する方法 - Google Patents

ウイルスを単離する方法 Download PDF

Info

Publication number
JP5827948B2
JP5827948B2 JP2012525057A JP2012525057A JP5827948B2 JP 5827948 B2 JP5827948 B2 JP 5827948B2 JP 2012525057 A JP2012525057 A JP 2012525057A JP 2012525057 A JP2012525057 A JP 2012525057A JP 5827948 B2 JP5827948 B2 JP 5827948B2
Authority
JP
Japan
Prior art keywords
virus
sample
atoms
methylimidazolium
centrifugation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2012525057A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013502206A5 (enExample
JP2013502206A (ja
Inventor
ロスマニート,ペーター
メスター,パトリック,ユリアン
ヒューン,シュテファン
ヴァーグナー,マルティン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of JP2013502206A publication Critical patent/JP2013502206A/ja
Publication of JP2013502206A5 publication Critical patent/JP2013502206A5/ja
Application granted granted Critical
Publication of JP5827948B2 publication Critical patent/JP5827948B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00Bacteriophages
    • C12N2795/00011Details
    • C12N2795/18011Details ssRNA Bacteriophages positive-sense
    • C12N2795/18111Leviviridae
    • C12N2795/18151Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
JP2012525057A 2009-08-17 2010-07-16 ウイルスを単離する方法 Active JP5827948B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09010584 2009-08-17
EP09010584.2 2009-08-17
PCT/EP2010/004334 WO2011020530A2 (en) 2009-08-17 2010-07-16 Method for isolating viruses

Publications (3)

Publication Number Publication Date
JP2013502206A JP2013502206A (ja) 2013-01-24
JP2013502206A5 JP2013502206A5 (enExample) 2013-09-05
JP5827948B2 true JP5827948B2 (ja) 2015-12-02

Family

ID=42829612

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012525057A Active JP5827948B2 (ja) 2009-08-17 2010-07-16 ウイルスを単離する方法

Country Status (5)

Country Link
US (1) US20120141980A1 (enExample)
EP (1) EP2467471B1 (enExample)
JP (1) JP5827948B2 (enExample)
ES (1) ES2565335T3 (enExample)
WO (1) WO2011020530A2 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5529065B2 (ja) * 2011-03-29 2014-06-25 株式会社ミズホメディー イムノクロマトキット及び検出装置
GB201303666D0 (en) * 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
US10308989B2 (en) 2014-06-13 2019-06-04 North Carolina State University Aptamers with binding affinity to norovirus
JP2017112851A (ja) * 2015-12-21 2017-06-29 Dic株式会社 精製ウイルス液の製造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261764B1 (en) * 1997-09-22 2001-07-17 Chiron Corporation Buffers for stabilizing antigens
US6214221B1 (en) * 1999-02-22 2001-04-10 Henry B. Kopf Method and apparatus for purification of biological substances
JP2007522814A (ja) * 2004-02-23 2007-08-16 クルセル ホランド ベー ヴェー ウイルスの精製方法
WO2008017097A1 (en) * 2006-08-10 2008-02-14 Merck Patent Gmbh Method for isolating cells
CA2629589C (en) * 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase

Also Published As

Publication number Publication date
EP2467471B1 (en) 2016-01-13
ES2565335T3 (es) 2016-04-04
WO2011020530A2 (en) 2011-02-24
WO2011020530A3 (en) 2011-05-26
EP2467471A2 (en) 2012-06-27
US20120141980A1 (en) 2012-06-07
JP2013502206A (ja) 2013-01-24

Similar Documents

Publication Publication Date Title
JP7449266B2 (ja) アルカリホスファターゼの下流プロセシング
JP5827948B2 (ja) ウイルスを単離する方法
JPWO2007105731A1 (ja) 氷核形成活性を有するタンパク質
HRP20010113A2 (en) Process for purifying human papillomavirus virus-like particles
EP2049677A1 (en) Method for isolating cells
Venter Molecular differentiation and pathogenicity of Aviadenoviruses isolated during an outbreak of inclusion body hepatitis in South Africa
US20130337462A1 (en) Extraction of nucleic acids
CA2592495A1 (en) Use of flexible bag containers for viral production
CN110101864A (zh) 一种无血清人用狂犬病疫苗的保护剂及其应用
CN102178945B (zh) 一种石斑鱼虹彩病毒灭活疫苗的制备方法
US20220183316A1 (en) Food products comprising cultivated bovine cells and methods thereof
Zheng et al. Identification of two novel C-type lectins involved in immune defense against white spot syndrome virus and Vibrio parahaemolyticus from Marsupenaeus japonicus
Du et al. Autophagy and apoptosis induced by Chinese giant salamander (Andrias davidianus) iridovirus (CGSIV)
Huang et al. Characterization of four spliced isoforms of a transmembrane C-type lectin from Procambarus clarkii and their function in facilitating WSSV infection
CN117363657A (zh) 一种慢病毒载体保存液、慢病毒载体制剂及其制备方法
EP2853587B1 (en) Method for isolating cells
Wang et al. High cell-density fermentation, expression and purification of bacteriophage lysin TSPphg, a thermostable antimicrobial protein from extremophilic Thermus bacteriophage TSP4
JP4372421B2 (ja) 細胞からタンパク質を抽出するための方法および組成物
JP7011132B2 (ja) 新規キトサナーゼchi1、そのコード遺伝子およびその使用
CN106879884B (zh) 一种提高蜂王浆性能的方法
JP2019518061A (ja) 口蹄疫ウイルス不活化抗原の精製と濃縮方法
Zhou et al. The primary culture of mirror carp snout and caudal fin tissues and the isolation of Koi herpesvirus
CN105237627A (zh) 一种低分子量牛乳铁蛋白肽抗菌肽的纯化方法
JP2006124295A (ja) 不凍タンパク質混合物
CN102433341A (zh) 一种斜带石斑鱼抗菌肽的原核表达产物及其制备方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130716

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130716

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20141007

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20141222

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150407

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150918

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20151019

R150 Certificate of patent or registration of utility model

Ref document number: 5827948

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250