JP5769374B2 - 標的を定めた変異誘発によって改変された変異δ８デサチュラーゼ遺伝子及び多価不飽和脂肪酸の製造におけるそれらの使用 - Google Patents

標的を定めた変異誘発によって改変された変異δ８デサチュラーゼ遺伝子及び多価不飽和脂肪酸の製造におけるそれらの使用 Download PDF

Info

Publication number
JP5769374B2
JP5769374B2 JP2009540294A JP2009540294A JP5769374B2 JP 5769374 B2 JP5769374 B2 JP 5769374B2 JP 2009540294 A JP2009540294 A JP 2009540294A JP 2009540294 A JP2009540294 A JP 2009540294A JP 5769374 B2 JP5769374 B2 JP 5769374B2
Authority
JP
Japan
Prior art keywords
desaturase
egd8s
seq
sequence
pfmd8s
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2009540294A
Other languages
English (en)
Japanese (ja)
Other versions
JP2010512146A (ja
JP2010512146A5 (enExample
Inventor
ハワード グレン ダムード
ハワード グレン ダムード
ホンシアン ヒー
ホンシアン ヒー
デル−イン リアオ
デル−イン リアオ
クイン クン ズー
クイン クン ズー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of JP2010512146A publication Critical patent/JP2010512146A/ja
Publication of JP2010512146A5 publication Critical patent/JP2010512146A5/ja
Application granted granted Critical
Publication of JP5769374B2 publication Critical patent/JP5769374B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2009540294A 2006-12-07 2007-12-05 標的を定めた変異誘発によって改変された変異δ８デサチュラーゼ遺伝子及び多価不飽和脂肪酸の製造におけるそれらの使用 Expired - Fee Related JP5769374B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/635,258 2006-12-07
US11/635,258 US7709239B2 (en) 2006-12-07 2006-12-07 Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
PCT/US2007/025001 WO2008073271A1 (en) 2006-12-07 2007-12-05 Mutant delta8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids

Publications (3)

Publication Number Publication Date
JP2010512146A JP2010512146A (ja) 2010-04-22
JP2010512146A5 JP2010512146A5 (enExample) 2011-01-27
JP5769374B2 true JP5769374B2 (ja) 2015-08-26

Family

ID=39269299

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2009540294A Expired - Fee Related JP5769374B2 (ja) 2006-12-07 2007-12-05 標的を定めた変異誘発によって改変された変異δ８デサチュラーゼ遺伝子及び多価不飽和脂肪酸の製造におけるそれらの使用

Country Status (8)

Country Link
US (2) US7709239B2 (enExample)
EP (1) EP2087106B1 (enExample)
JP (1) JP5769374B2 (enExample)
CN (1) CN101627118B (enExample)
AU (1) AU2007332918B2 (enExample)
CA (1) CA2669217C (enExample)
DK (1) DK2087106T3 (enExample)
WO (1) WO2008073271A1 (enExample)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2529572T3 (es) 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
EP2363492A3 (en) 2004-04-22 2012-03-14 Commonwealth Scientific and Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells.
AU2007291937B2 (en) 2006-08-29 2014-05-15 Commonwealth Scientific And Industrial Research Organisation Synthesis of fatty acids
US8188338B2 (en) * 2007-04-10 2012-05-29 E. I. Du Pont De Nemours And Company Delta-8 desaturases and their use in making polyunsaturated fatty acids
US8323935B2 (en) * 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
MX2011003700A (es) * 2008-10-06 2011-11-29 Abbott Lab Genes de delta-8 desaturasa, enzimas codificadas por los mismos y usos de los mismos.
CN113957054B (zh) 2008-11-18 2024-07-16 联邦科学技术研究组织 产生ω-3脂肪酸的酶和方法
WO2010080388A1 (en) 2008-12-18 2010-07-15 E. I. Du Pont De Nemours And Company Reducing byproduction of malonates in a fermentation process
DK2443248T3 (en) 2009-06-16 2018-03-12 Du Pont IMPROVEMENT OF LONG-CHAIN POLYUM Saturated OMEGA-3 AND OMEGA-6 FATTY ACID BIOS SYNTHESIS BY EXPRESSION OF ACYL-CoA LYSOPHOSPHOLIPID ACYL TRANSFERASES
DK2442666T3 (da) 2009-06-16 2015-07-13 Du Pont Olier med højt indhold af eicosapentaensyre fra forbedrede og optimerede stammer af yarrowia lipolytica
CA2761589C (en) 2009-06-16 2017-12-19 E. I. Du Pont De Nemours And Company Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
WO2011087981A2 (en) 2010-01-15 2011-07-21 E. I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
WO2011133610A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
EP2603092A1 (en) 2010-08-11 2013-06-19 E.I. Du Pont De Nemours And Company Improved aquaculture meat products
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
US20120204802A1 (en) 2010-08-11 2012-08-16 E. I. Du Pont De Nemours And Company Sustainable aquaculture feeding strategy
CA2808002A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids
DK2609203T3 (da) 2010-08-26 2018-10-01 Du Pont Rekombinante mikrobielle værtsceller til større fremstilling af eicosapentaensyre
WO2012027676A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
MX2013004944A (es) * 2010-11-05 2013-06-28 Basf Plant Science Co Gmbh Metodo para aumentar el rendimiento y la produccion de quimicos finos en las plantas.
KR20140000711A (ko) 2010-12-30 2014-01-03 이 아이 듀폰 디 네모아 앤드 캄파니 수크로스 이용에 있어서의 야로위아 리포라이티카에서의 사카로마이세스 세레비지애 suc2 유전자의 용도
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
US8969049B2 (en) 2011-03-31 2015-03-03 E I Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
EP2694665A1 (en) 2011-04-01 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
US8735094B2 (en) 2011-04-05 2014-05-27 E I Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome P450 promoter regions for gene expression in yeast
EP2702148A1 (en) 2011-04-07 2014-03-05 E.I. Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-coa reductase promoter regions for gene expression in yeast
EA036786B1 (ru) 2012-06-15 2020-12-21 Коммонвелт Сайнтифик Энд Индастриэл Рисерч Организэйшн Выработка длинноцепочечных полиненасыщенных жирных кислот в растительных клетках
CA2892516A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
EP3082405A4 (en) 2013-12-18 2017-12-13 Commonwealth Scientific and Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
CN105219789B (zh) 2014-06-27 2023-04-07 联邦科学技术研究组织 包含二十二碳五烯酸的提取的植物脂质
CN104293769B (zh) * 2014-10-27 2016-06-01 徐毅 一种来源于绿色巴夫藻的delta8去饱和酶新基因
EP4407026A3 (en) * 2019-05-28 2025-01-15 DNA Script Variants of terminal deoxynucleotidyl transferase and uses thereof

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69935162T2 (de) 1998-12-07 2007-11-22 Washington State University Research Foundation, Pullman Desaturasen und verfahren ihrer anwendung zur synthese von mehrfach ungesättigten fettsäuren
US6825017B1 (en) * 1998-12-07 2004-11-30 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
BR0317304A (pt) * 2002-12-19 2005-11-08 Univ Bristol Processo para a produção de compostos, sequência de ácido nucleico isolada, sequência de aminoácidos, construção gênica, vetor, e, organismo
US7129089B2 (en) * 2003-02-12 2006-10-31 E. I. Du Pont De Nemours And Company Annexin and P34 promoters and use in expression of transgenic genes in plants
US20040172682A1 (en) * 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) * 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US7259255B2 (en) * 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7459546B2 (en) * 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US7267976B2 (en) * 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
FR2857322B1 (fr) * 2003-07-10 2006-08-11 Nacam Dispositif d'absorption d'energie infiniment modulable d'une colonne de direction de vehicule automobile
CA3037924A1 (en) 2003-08-01 2005-02-10 Basf Plant Science Gmbh Method for the production of polyunsaturated fatty acids in transgenic organisms
KR20050036190A (ko) * 2003-10-15 2005-04-20 삼성전자주식회사 플립플롭
US7504259B2 (en) * 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
WO2005047479A2 (en) * 2003-11-12 2005-05-26 E.I. Dupont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast
WO2005049805A2 (en) * 2003-11-14 2005-06-02 E.I. Dupont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
EP4219670A3 (de) 2004-02-27 2023-08-09 BASF Plant Science GmbH Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen pflanzen
EP2363492A3 (en) 2004-04-22 2012-03-14 Commonwealth Scientific and Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells.
ES2529572T3 (es) * 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
DK1766023T3 (da) * 2004-06-04 2010-12-13 Fluxome Sciences As Metabolisk manipulerede celler til fremstillingen af polyumættede fedtsyrer
WO2006012325A1 (en) 2004-06-25 2006-02-02 E.I. Dupont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7465564B2 (en) * 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7264949B2 (en) * 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US7588931B2 (en) * 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
US20060094102A1 (en) * 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
US7189559B2 (en) * 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7192762B2 (en) * 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7273746B2 (en) * 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7198937B2 (en) * 2004-11-04 2007-04-03 E. I. Du Pont De Nemours And Company Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US8685679B2 (en) * 2004-11-04 2014-04-01 E I Du Pont De Nemours And Company Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
US7470532B2 (en) * 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
AU2006316610B2 (en) * 2005-11-23 2012-06-14 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7465793B2 (en) * 2006-04-20 2008-12-16 E.I. Du Pont De Nemours And Company Synthetic Δ17 desaturase derived from Phytophthora ramourum and its use in making polyunsaturated fatty acids
US7943823B2 (en) 2006-04-28 2011-05-17 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
EP2013335A2 (en) * 2006-05-01 2009-01-14 E.I. Du Pont De Nemours And Company Fungal delta12 desaturase and delta15 desaturase motifs
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids

Also Published As

Publication number Publication date
AU2007332918B2 (en) 2013-02-07
JP2010512146A (ja) 2010-04-22
EP2087106A1 (en) 2009-08-12
US8026089B2 (en) 2011-09-27
CN101627118A (zh) 2010-01-13
CA2669217C (en) 2015-07-14
AU2007332918A1 (en) 2008-06-19
EP2087106B1 (en) 2015-03-04
US7709239B2 (en) 2010-05-04
DK2087106T3 (en) 2015-06-08
WO2008073271A9 (en) 2009-04-30
CN101627118B (zh) 2013-07-17
US20080138868A1 (en) 2008-06-12
WO2008073271A1 (en) 2008-06-19
CA2669217A1 (en) 2008-06-19
US20100297713A1 (en) 2010-11-25

Similar Documents

Publication Publication Date Title
JP5769374B2 (ja) 標的を定めた変異誘発によって改変された変異δ８デサチュラーゼ遺伝子及び多価不飽和脂肪酸の製造におけるそれらの使用
JP5501952B2 (ja) Δ８デサチュラーゼ、および多価不飽和脂肪酸の作成におけるそれらの使用
EP2021478B1 (en) Delta5 desaturase and its use in making polyunsaturated fatty acids
JP5193216B2 (ja) Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるその使用
JP5501954B2 (ja) Δ９エロンガーゼおよび多価不飽和脂肪酸の作成におけるそれらの使用
JP5762291B2 (ja) 変異δ５デサチュラーゼと、多価不飽和脂肪酸の製造におけるそれらの使用
US7695950B2 (en) Δ5 desaturase and its use in making polyunsaturated fatty acids
JP5572620B2 (ja) Δ４デサチュラーゼ、および多価不飽和脂肪酸の製造におけるその使用
EP2140006A1 (en) Delta-5 desaturases and their use in making polyunsaturated fatty acids
JP2009534032A (ja) Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
US7923223B2 (en) Δ-9 desaturase and its use in making polyunsaturated fatty acids
JP2011529689A (ja) Δ６デサチュラーゼおよび多価不飽和脂肪酸生成におけるそれらの使用
WO2008073275A2 (en) Mutant delta-8 desaturase genes engineered by targeted mutagensis and their use in making polyunsaturated fatty acids

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20100419

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20101130

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20101130

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20121112

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130212

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20131015

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140217

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140402

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20140408

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20140516

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150623

R150 Certificate of patent or registration of utility model

Ref document number: 5769374

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees