JP5769374B2 - 標的を定めた変異誘発によって改変された変異δ８デサチュラーゼ遺伝子及び多価不飽和脂肪酸の製造におけるそれらの使用 - Google Patents

標的を定めた変異誘発によって改変された変異δ８デサチュラーゼ遺伝子及び多価不飽和脂肪酸の製造におけるそれらの使用 Download PDF

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JP5769374B2
JP5769374B2 JP2009540294A JP2009540294A JP5769374B2 JP 5769374 B2 JP5769374 B2 JP 5769374B2 JP 2009540294 A JP2009540294 A JP 2009540294A JP 2009540294 A JP2009540294 A JP 2009540294A JP 5769374 B2 JP5769374 B2 JP 5769374B2
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desaturase
egd8s
seq
sequence
pfmd8s
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JP2010512146A5 (cg-RX-API-DMAC7.html
JP2010512146A (ja
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ハワード グレン ダムード
ハワード グレン ダムード
ホンシアン ヒー
ホンシアン ヒー
デル−イン リアオ
デル−イン リアオ
クイン クン ズー
クイン クン ズー
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)

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  • Health & Medical Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2009540294A 2006-12-07 2007-12-05 標的を定めた変異誘発によって改変された変異δ８デサチュラーゼ遺伝子及び多価不飽和脂肪酸の製造におけるそれらの使用 Expired - Fee Related JP5769374B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/635,258 US7709239B2 (en) 2006-12-07 2006-12-07 Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US11/635,258 2006-12-07
PCT/US2007/025001 WO2008073271A1 (en) 2006-12-07 2007-12-05 Mutant delta8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids

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JP2010512146A JP2010512146A (ja) 2010-04-22
JP2010512146A5 JP2010512146A5 (cg-RX-API-DMAC7.html) 2011-01-27
JP5769374B2 true JP5769374B2 (ja) 2015-08-26

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US (2) US7709239B2 (cg-RX-API-DMAC7.html)
EP (1) EP2087106B1 (cg-RX-API-DMAC7.html)
JP (1) JP5769374B2 (cg-RX-API-DMAC7.html)
CN (1) CN101627118B (cg-RX-API-DMAC7.html)
AU (1) AU2007332918B2 (cg-RX-API-DMAC7.html)
CA (1) CA2669217C (cg-RX-API-DMAC7.html)
DK (1) DK2087106T3 (cg-RX-API-DMAC7.html)
WO (1) WO2008073271A1 (cg-RX-API-DMAC7.html)

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AU2009317860B2 (en) 2008-11-18 2014-03-27 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
US9175318B2 (en) 2008-12-18 2015-11-03 E. I. Dupont De Nemours And Company Reducing byproduction of malonates by yeast in a fermentation process
CA2763424C (en) 2009-06-16 2018-06-12 E.I. Du Pont De Nemours And Company Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-coa lysophospholipid acyltransferases
EP2475679B1 (en) 2009-06-16 2016-02-10 E. I. du Pont de Nemours and Company Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
CN102595917A (zh) 2009-06-16 2012-07-18 纳幕尔杜邦公司 来自改善的优化的解脂耶氏酵母菌株的高二十碳五烯酸油
US20110178105A1 (en) 2010-01-15 2011-07-21 E.I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
CA2795460A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
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KR20140008295A (ko) 2010-08-26 2014-01-21 이 아이 듀폰 디 네모아 앤드 캄파니 돌연변이 델타-9 연장효소 및 다중불포화 지방산의 제조에서의 그들의 용도
US8658413B2 (en) 2010-08-26 2014-02-25 E I Du Pont De Nemours And Company Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids
AU2011293180B2 (en) 2010-08-26 2017-03-02 E. I. Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
MX2013004944A (es) * 2010-11-05 2013-06-28 Basf Plant Science Co Gmbh Metodo para aumentar el rendimiento y la produccion de quimicos finos en las plantas.
US8735137B2 (en) 2010-12-30 2014-05-27 E I Du Pont De Nemours And Company Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
WO2012135773A1 (en) 2011-03-31 2012-10-04 E. I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
EP2694665A1 (en) 2011-04-01 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
WO2012138613A1 (en) 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
US8609369B2 (en) 2011-04-07 2013-12-17 E I Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast
US8816111B2 (en) 2012-06-15 2014-08-26 Commonwealth Scientific And Industrial Research Organisation Lipid comprising polyunsaturated fatty acids
EP2935591B1 (en) 2012-12-21 2017-07-05 E. I. du Pont de Nemours and Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
WO2015196250A1 (en) 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
AU2014366839B2 (en) 2013-12-18 2021-07-01 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
CN104293769B (zh) * 2014-10-27 2016-06-01 徐毅 一种来源于绿色巴夫藻的delta8去饱和酶新基因
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US7238482B2 (en) * 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
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US7264949B2 (en) * 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US7192762B2 (en) * 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
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US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids

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US20080138868A1 (en) 2008-06-12
WO2008073271A1 (en) 2008-06-19
CA2669217A1 (en) 2008-06-19
CA2669217C (en) 2015-07-14
US7709239B2 (en) 2010-05-04
EP2087106B1 (en) 2015-03-04
AU2007332918A1 (en) 2008-06-19
AU2007332918B2 (en) 2013-02-07
JP2010512146A (ja) 2010-04-22
WO2008073271A9 (en) 2009-04-30
US20100297713A1 (en) 2010-11-25
DK2087106T3 (en) 2015-06-08
US8026089B2 (en) 2011-09-27
CN101627118B (zh) 2013-07-17
CN101627118A (zh) 2010-01-13
EP2087106A1 (en) 2009-08-12

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