AU2007332918B2 - Mutant delta8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids - Google Patents

Mutant delta8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids Download PDF

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Publication number
AU2007332918B2
AU2007332918B2 AU2007332918A AU2007332918A AU2007332918B2 AU 2007332918 B2 AU2007332918 B2 AU 2007332918B2 AU 2007332918 A AU2007332918 A AU 2007332918A AU 2007332918 A AU2007332918 A AU 2007332918A AU 2007332918 B2 AU2007332918 B2 AU 2007332918B2
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Australia
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seq
desaturase
acid
sequence
polypeptide
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Ceased
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AU2007332918A
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AU2007332918A1 (en
Inventor
Howard Glenn Damude
Hongxian He
Der-Ing Liao
Quinn Qun Zhu
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083Miscellaneous (1.14.99)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2007332918A 2006-12-07 2007-12-05 Mutant delta8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids Ceased AU2007332918B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/635,258 US7709239B2 (en) 2006-12-07 2006-12-07 Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US11/635,258 2006-12-07
PCT/US2007/025001 WO2008073271A1 (en) 2006-12-07 2007-12-05 Mutant delta8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids

Publications (2)

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AU2007332918A1 AU2007332918A1 (en) 2008-06-19
AU2007332918B2 true AU2007332918B2 (en) 2013-02-07

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AU2007332918A Ceased AU2007332918B2 (en) 2006-12-07 2007-12-05 Mutant delta8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids

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US (2) US7709239B2 (cg-RX-API-DMAC7.html)
EP (1) EP2087106B1 (cg-RX-API-DMAC7.html)
JP (1) JP5769374B2 (cg-RX-API-DMAC7.html)
CN (1) CN101627118B (cg-RX-API-DMAC7.html)
AU (1) AU2007332918B2 (cg-RX-API-DMAC7.html)
CA (1) CA2669217C (cg-RX-API-DMAC7.html)
DK (1) DK2087106T3 (cg-RX-API-DMAC7.html)
WO (1) WO2008073271A1 (cg-RX-API-DMAC7.html)

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CN101970638B (zh) * 2007-10-03 2015-02-11 纳幕尔杜邦公司 用于高水平生产二十碳五烯酸的优化解脂耶氏酵母菌株
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AU2009317860B2 (en) 2008-11-18 2014-03-27 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
US9175318B2 (en) 2008-12-18 2015-11-03 E. I. Dupont De Nemours And Company Reducing byproduction of malonates by yeast in a fermentation process
CA2763424C (en) 2009-06-16 2018-06-12 E.I. Du Pont De Nemours And Company Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-coa lysophospholipid acyltransferases
EP2475679B1 (en) 2009-06-16 2016-02-10 E. I. du Pont de Nemours and Company Improved optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
CN102595917A (zh) 2009-06-16 2012-07-18 纳幕尔杜邦公司 来自改善的优化的解脂耶氏酵母菌株的高二十碳五烯酸油
US20110178105A1 (en) 2010-01-15 2011-07-21 E.I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
CA2795460A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
CA2805891A1 (en) 2010-08-11 2012-02-16 Scott E. Nichols A sustainable aquaculture feeding strategy
CA2805882A1 (en) 2010-08-11 2012-02-16 E. I. Dupont De Nemours And Company Improved aquaculture meat products
KR20140008295A (ko) 2010-08-26 2014-01-21 이 아이 듀폰 디 네모아 앤드 캄파니 돌연변이 델타-9 연장효소 및 다중불포화 지방산의 제조에서의 그들의 용도
US8658413B2 (en) 2010-08-26 2014-02-25 E I Du Pont De Nemours And Company Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids
AU2011293180B2 (en) 2010-08-26 2017-03-02 E. I. Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
MX2013004944A (es) * 2010-11-05 2013-06-28 Basf Plant Science Co Gmbh Metodo para aumentar el rendimiento y la produccion de quimicos finos en las plantas.
US8735137B2 (en) 2010-12-30 2014-05-27 E I Du Pont De Nemours And Company Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
WO2012135773A1 (en) 2011-03-31 2012-10-04 E. I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
EP2694665A1 (en) 2011-04-01 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
WO2012138613A1 (en) 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
US8609369B2 (en) 2011-04-07 2013-12-17 E I Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast
US8816111B2 (en) 2012-06-15 2014-08-26 Commonwealth Scientific And Industrial Research Organisation Lipid comprising polyunsaturated fatty acids
EP2935591B1 (en) 2012-12-21 2017-07-05 E. I. du Pont de Nemours and Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
WO2015196250A1 (en) 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
AU2014366839B2 (en) 2013-12-18 2021-07-01 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
CN104293769B (zh) * 2014-10-27 2016-06-01 徐毅 一种来源于绿色巴夫藻的delta8去饱和酶新基因
EP4407026A3 (en) * 2019-05-28 2025-01-15 DNA Script Variants of terminal deoxynucleotidyl transferase and uses thereof

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US6825017B1 (en) * 1998-12-07 2004-11-30 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
AU2003296638B2 (en) * 2002-12-19 2009-06-11 University Of Bristol Method for the production of polyunsaturated fatty acids
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US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
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US7267976B2 (en) * 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
FR2857322B1 (fr) * 2003-07-10 2006-08-11 Nacam Dispositif d'absorption d'energie infiniment modulable d'une colonne de direction de vehicule automobile
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EP1758991B1 (en) * 2004-06-25 2013-01-16 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7465564B2 (en) * 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7264949B2 (en) * 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US7192762B2 (en) * 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7588931B2 (en) * 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
US20060094102A1 (en) * 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
US7189559B2 (en) * 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7273746B2 (en) * 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
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Patent Citations (1)

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US20080138868A1 (en) 2008-06-12
JP5769374B2 (ja) 2015-08-26
WO2008073271A1 (en) 2008-06-19
CA2669217A1 (en) 2008-06-19
CA2669217C (en) 2015-07-14
US7709239B2 (en) 2010-05-04
EP2087106B1 (en) 2015-03-04
AU2007332918A1 (en) 2008-06-19
JP2010512146A (ja) 2010-04-22
WO2008073271A9 (en) 2009-04-30
US20100297713A1 (en) 2010-11-25
DK2087106T3 (en) 2015-06-08
US8026089B2 (en) 2011-09-27
CN101627118B (zh) 2013-07-17
CN101627118A (zh) 2010-01-13
EP2087106A1 (en) 2009-08-12

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