JP5753381B2 - クロストリジウム・ディフィシレの毒素原性菌株の検出 - Google Patents

クロストリジウム・ディフィシレの毒素原性菌株の検出 Download PDF

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JP5753381B2
JP5753381B2 JP2010523247A JP2010523247A JP5753381B2 JP 5753381 B2 JP5753381 B2 JP 5753381B2 JP 2010523247 A JP2010523247 A JP 2010523247A JP 2010523247 A JP2010523247 A JP 2010523247A JP 5753381 B2 JP5753381 B2 JP 5753381B2
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pcr
amplification
primer
sequence
primers
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JP2010537648A (ja
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ナンシー パケット、
ナンシー パケット、
マリー−エヴァ ロシェット、
マリー−エヴァ ロシェット、
ラシェル ラブールデット、
ラシェル ラブールデット、
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ジェネオーム サイエンシズ カナダ、 インク．
ジェネオーム サイエンシズ カナダ、 インク．
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/101—Taqman
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2010523247A 2007-09-06 2008-09-05 クロストリジウム・ディフィシレの毒素原性菌株の検出 Active JP5753381B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US97049207P 2007-09-06 2007-09-06
US60/970,492 2007-09-06
PCT/CA2008/001564 WO2009030031A1 (en) 2007-09-06 2008-09-05 Detection of toxigenic strains of clostridium difficile

Related Child Applications (1)

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JP2014242047A Division JP5981979B2 (ja) 2007-09-06 2014-11-28 クロストリジウム・ディフィシレの毒素原性菌株の検出

Publications (2)

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JP2010537648A JP2010537648A (ja) 2010-12-09
JP5753381B2 true JP5753381B2 (ja) 2015-07-22

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JP2010523247A Active JP5753381B2 (ja) 2007-09-06 2008-09-05 クロストリジウム・ディフィシレの毒素原性菌株の検出
JP2014242047A Active JP5981979B2 (ja) 2007-09-06 2014-11-28 クロストリジウム・ディフィシレの毒素原性菌株の検出

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Country Link
US (2) US9096638B2 (https=)
EP (1) EP2195433B1 (https=)
JP (2) JP5753381B2 (https=)
AU (1) AU2008295396C1 (https=)
CA (1) CA2698232C (https=)
WO (1) WO2009030031A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9096638B2 (en) * 2007-09-06 2015-08-04 Geneohm Sciences Canada, Inc. Detection of toxigenic strains of Clostridium difficile
KR101798211B1 (ko) * 2009-04-07 2017-11-15 코닌클리케 필립스 엔.브이. 독소생성 클로스트리디움 디피실리 균주의 검출 및 특성 확인 방법
US9234248B2 (en) 2009-07-17 2016-01-12 Brown University Simultaneous quantitative multiple primer detection of Clostridium difficile
WO2011100443A1 (en) * 2010-02-11 2011-08-18 Intelligent Medical Devices, Inc. Oligonucleotides relating to clostridium difficile genes encoding toxin b, toxin a, or binary toxin
TWI815599B (zh) 2011-04-22 2023-09-11 美商惠氏有限責任公司 與難養芽胞梭菌（Ｃｌｏｓｔｒｉｄｉｕｍ ｄｉｆｆｉｃｉｌｅ）之突變毒素有關之組成物及彼之方法
EP2752496B1 (en) * 2011-09-01 2018-05-23 Kabushiki Kaisha Yakult Honsha Method for detecting toxin-producing clostridium difficile
EP2831260B1 (en) * 2012-03-29 2018-10-17 Becton, Dickinson and Company Nucleic acids for nucleic acid amplification
KR101955329B1 (ko) 2012-06-08 2019-03-07 삼성전자주식회사 클로스트리디움 디피실리 균주 검출용 조성물과 키트 및 이를 이용한 검출 방법
US9080217B2 (en) 2012-09-21 2015-07-14 Roche Molecular Systems, Inc. Methods for detection of Clostridium difficile
BR122016023101B1 (pt) 2012-10-21 2022-03-22 Pfizer Inc Polipeptídeo, composição imunogênica que o compreende, bem como célula recombinante derivada de clostridium difficile
CN102952886A (zh) * 2012-11-28 2013-03-06 中华人民共和国张家港出入境检验检疫局 艰难梭菌肠毒素a、b双重荧光定量pcr检测方法及检测用试剂盒
EP2989212B1 (en) * 2013-04-25 2018-07-18 Orion Diagnostica Oy Strand-invasion based dna amplification method
JP7176266B2 (ja) * 2018-07-18 2022-11-22 東洋紡株式会社 トキシンＢ遺伝子（ｔｃｄＢ）を検出するためのオリゴヌクレオチドプローブ
US11597961B2 (en) 2019-04-22 2023-03-07 Qualitic Biotechnology LLC Rapid colorimetric diagnostic test for C. difficile
WO2021255690A2 (en) 2020-06-19 2021-12-23 Pfizer Inc. Immunogenic compositions against clostridioides (clostridium) difficile and methods thereof
CN115948584A (zh) * 2022-12-16 2023-04-11 新疆维吾尔自治区疾病预防控制中心 利用CRISPR-Cas12a蛋白检测艰难梭菌的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1246935B1 (en) * 1999-09-28 2013-08-14 Geneohm Sciences Canada Inc. Highly conserved genes and their use to generate probes and primers for detection of microorganisms
JP4899009B2 (ja) 2005-06-02 2012-03-21 財団法人ヒューマンサイエンス振興財団 ＬＡＭＰ法を用いたクロストリディウム・ディフィシルｔｏｘｉｎＢ遺伝子検出方法およびこの方法に用いるプライマーセット
JPWO2008041354A1 (ja) 2006-10-03 2010-02-04 国立大学法人岐阜大学 ＤｎａＪ遺伝子を利用した細菌の検出及びその利用
US9096638B2 (en) * 2007-09-06 2015-08-04 Geneohm Sciences Canada, Inc. Detection of toxigenic strains of Clostridium difficile

Also Published As

Publication number Publication date
US9863007B2 (en) 2018-01-09
WO2009030031A1 (en) 2009-03-12
EP2195433A1 (en) 2010-06-16
CA2698232A1 (en) 2009-03-12
EP2195433B1 (en) 2014-12-24
JP2010537648A (ja) 2010-12-09
US20150292004A1 (en) 2015-10-15
EP2195433A4 (en) 2011-05-18
US9096638B2 (en) 2015-08-04
AU2008295396A2 (en) 2010-05-13
JP5981979B2 (ja) 2016-08-31
JP2015096066A (ja) 2015-05-21
AU2008295396C1 (en) 2015-07-02
AU2008295396A1 (en) 2009-03-12
US20090208948A1 (en) 2009-08-20
AU2008295396B2 (en) 2015-01-29
CA2698232C (en) 2018-06-12

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