JP5670755B2 - 翻訳プロファイリング及び分子表現型解析のための方法及び組成物 - Google Patents

翻訳プロファイリング及び分子表現型解析のための方法及び組成物 Download PDF

Info

Publication number
JP5670755B2
JP5670755B2 JP2010550699A JP2010550699A JP5670755B2 JP 5670755 B2 JP5670755 B2 JP 5670755B2 JP 2010550699 A JP2010550699 A JP 2010550699A JP 2010550699 A JP2010550699 A JP 2010550699A JP 5670755 B2 JP5670755 B2 JP 5670755B2
Authority
JP
Japan
Prior art keywords
cell
cells
gene
expression
mrna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2010550699A
Other languages
English (en)
Japanese (ja)
Other versions
JP2011512871A (ja
JP2011512871A5 (https=
Inventor
ナタニエル ハインツ，
ナタニエル ハインツ，
ポール グリーンガード，
ポール グリーンガード，
ミリアン ヘイマン，
ミリアン ヘイマン，
アン シェイファー，
アン シェイファー，
ジョセフ ピー．エヌ ドイル，
ジョセフ ピー．エヌ ドイル，
ジョセフ ディー． ドーハーティ，
ジョセフ ディー． ドーハーティ，
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rockefeller University
Original Assignee
Rockefeller University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rockefeller University filed Critical Rockefeller University
Publication of JP2011512871A publication Critical patent/JP2011512871A/ja
Publication of JP2011512871A5 publication Critical patent/JP2011512871A5/ja
Application granted granted Critical
Publication of JP5670755B2 publication Critical patent/JP5670755B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/206—Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/285—Demyelinating diseases; Multipel sclerosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Environmental Sciences (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2010550699A 2008-03-12 2009-03-12 翻訳プロファイリング及び分子表現型解析のための方法及び組成物 Expired - Fee Related JP5670755B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US3604908P 2008-03-12 2008-03-12
US3605808P 2008-03-12 2008-03-12
US61/036,058 2008-03-12
US61/036,049 2008-03-12
US7032708P 2008-03-21 2008-03-21
US61/070,327 2008-03-21
US19910808P 2008-11-12 2008-11-12
US61/199,108 2008-11-12
PCT/US2009/001614 WO2009114185A2 (en) 2008-03-12 2009-03-12 Methods and compositions for translational profiling and molecular phenotyping

Publications (3)

Publication Number Publication Date
JP2011512871A JP2011512871A (ja) 2011-04-28
JP2011512871A5 JP2011512871A5 (https=) 2013-04-04
JP5670755B2 true JP5670755B2 (ja) 2015-02-18

Family

ID=41065731

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010550699A Expired - Fee Related JP5670755B2 (ja) 2008-03-12 2009-03-12 翻訳プロファイリング及び分子表現型解析のための方法及び組成物

Country Status (4)

Country Link
US (3) US20110071049A1 (https=)
EP (2) EP3369827B1 (https=)
JP (1) JP5670755B2 (https=)
WO (1) WO2009114185A2 (https=)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002350039A1 (en) 2001-10-29 2003-05-12 Renovis, Inc. Method for isolating cell-type specific mrnas
EP3369827B1 (en) 2008-03-12 2020-12-23 The Rockefeller University Methods and compositions for translational profiling and molecular phenotyping
EP2271358B1 (en) * 2008-03-25 2018-11-21 Amarantus Therapeutics, Inc. use of mesencephalic astrocyte-derived neurotrophic factor for treating Parkinson's disease
US10267796B2 (en) * 2010-10-25 2019-04-23 The Procter & Gamble Company Screening methods of modulating adrenergic receptor gene expressions implicated in melanogenesis
AU2012223136B2 (en) * 2011-03-02 2017-05-25 Berg Llc Interrogatory cell-based assays and uses thereof
WO2012145459A2 (en) 2011-04-22 2012-10-26 International Park Of Creativity Glucose and insulin sensors and methods of use thereof
WO2013003801A2 (en) 2011-06-29 2013-01-03 The Trustees Of Columbia University In The City Of New York Inhibitor of neuronal connectivity linked to schizophrenia susceptibility and cognitive dysfunction
US20150141274A1 (en) * 2012-05-09 2015-05-21 The Rockefeller University Methods and Compositions for Activity Dependent Transcriptome Profiling
WO2014075093A1 (en) * 2012-11-09 2014-05-15 The Trustees Of Columbia University In The City Of New York Inhibitors of central nervous system vasoactive inhibitory peptide receptor 2
DK2954323T3 (da) * 2013-02-07 2020-07-20 Univ California Anvendelse af translationsprofilering til identifikation af målmolekyler til terapeutisk behandling
EP2908137A1 (en) 2014-02-14 2015-08-19 Institut Pasteur Methods for in vitro investigating mitochondrial replication dysfunction in a biological sample, kits and uses thereof, therapeutic methods against progeroid-like syndromes or symptomes and screening method for identifying particular protease inhibitor(s) and/or nitroso-redox stress scavenger compound(s)
JP7019590B2 (ja) 2016-03-31 2022-02-15 バークレー ライツ，インコーポレイテッド 核酸安定化試薬、キット、及びその使用方法
SE540173C2 (en) * 2016-04-20 2018-04-24 Hiloprobe Ab Marker genes for colorectal cancer classification, method for judging lymph node metastasis for prognosis of colorectal cancer and kit therefor
IL268417B2 (en) * 2017-02-10 2025-05-01 Univ Rockefeller Methods for cell-type specific profiling to identify drug targets
US12590288B2 (en) 2017-04-12 2026-03-31 The Broad Institute, Inc. Method of treating an inflammatory disease by administering an agent which binds a surface receptor on a tuft cell that induces an ILC class 2 inflammatory response
US11227692B2 (en) * 2017-12-28 2022-01-18 International Business Machines Corporation Neuron model simulation
CN109813912B (zh) * 2019-01-04 2021-12-28 深圳大学 一组血清差异蛋白组合在制备用于检测孤独症的试剂中的应用
CN113621621A (zh) * 2021-08-31 2021-11-09 河南牧业经济学院 一种靶向奶山羊CIDEa基因的小干扰RNA及其应用
CN114350705A (zh) * 2021-11-16 2022-04-15 南方医科大学 一种转基因斑马鱼在制备特异标记原始红系造血过程的动物模型中的应用
CN114525241B (zh) * 2021-12-31 2023-06-20 中国人民解放军军事科学院军事医学研究院 SAFit2、培养基在促进人源诱导型多能干细胞向多巴胺能神经元分化中的应用
CN118947634B (zh) * 2024-09-30 2025-09-26 深圳市勤业医药科技有限公司 一种利用Cre-loxP系统来制备Scn1a+/-模型小鼠的新方法

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
US4870009A (en) 1982-11-22 1989-09-26 The Salk Institute For Biological Studies Method of obtaining gene product through the generation of transgenic animals
US4703004A (en) 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
US4736866B1 (en) 1984-06-22 1988-04-12 Transgenic non-human mammals
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US5075227A (en) * 1989-03-07 1991-12-24 Zymogenetics, Inc. Directional cloning
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
EP0945515A3 (en) 1989-11-06 2002-08-21 Cell Genesys, Inc. Production of proteins using homologous recombination
US5272071A (en) 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
DE4237113B4 (de) 1992-11-03 2006-10-12 "Iba Gmbh" Peptide und deren Fusionsproteine, Expressionsvektor und Verfahren zur Herstellung eines Fusionsproteins
US5795723A (en) * 1994-05-06 1998-08-18 Fred Hutchinson Cancer Research Center Expression of neurogenic bHLH genes in primitive neuroectodermal tumors
EP2322614A1 (en) * 1995-06-07 2011-05-18 Life Technologies Corporation Recombinational cloning using engineered recombination sites
GB9517780D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517779D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
EP0952767A4 (en) * 1995-11-13 2001-10-24 Univ Rochester PRODUCTION OF ONE SOMATIC MOSAIC IN MAMMALS USING A RECOMBINATIVE SUBSTRATE
US6495318B2 (en) * 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
US6124128A (en) * 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US6162965A (en) 1997-06-02 2000-12-19 Novartis Ag Plant transformation methods
EP0998574A1 (en) 1997-06-23 2000-05-10 The Rockefeller University Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acids
US6130090A (en) * 1997-06-23 2000-10-10 The Rockefeller University Methods of performing gene trapping in bacterial and bacteriophage-derived artificial chromosomes and use thereof
US6821759B1 (en) * 1997-06-23 2004-11-23 The Rockefeller University Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof
US6143566A (en) * 1997-06-23 2000-11-07 The Rockfeller University Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof
US6156574A (en) * 1997-06-23 2000-12-05 The Rockefeller University Methods of performing gene trapping in bacterial and bacteriophage-derived artificial chromosomes and use thereof
JP3736617B2 (ja) * 1997-12-12 2006-01-18 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 細胞型の決定方法
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
US7297482B2 (en) * 1998-10-08 2007-11-20 Rigel Pharmaceuticals, Inc. Structurally biased random peptide libraries based on different scaffolds
JP4475814B2 (ja) 1998-11-20 2010-06-09 扶桑薬品工業株式会社 タンパク質発現ベクターとその使用
AU781628B2 (en) * 1999-07-14 2005-06-02 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
AU6229800A (en) 1999-07-20 2001-02-05 Rockefeller University, The Conditional homologous recombination of large genomic vector inserts
NZ539430A (en) * 1999-12-10 2006-09-29 Invitrogen Corp Use of multiple recombination sites with unique specificity in recombinational cloning
US6635422B2 (en) * 1999-12-28 2003-10-21 Ribonomics, Inc. Methods for isolating and characterizing endogenous mRNA-protein (mRNP) complexes
MXPA02007787A (es) 2000-02-11 2004-09-10 Inst Genetics Llc Moleculas trade y uso relacionado con las mismas.
US20030051266A1 (en) 2001-02-14 2003-03-13 Serafini Tito Andrew Collections of transgenic animal lines (living library)
HUP0402659A3 (en) * 2001-05-30 2008-07-28 Glaxo Group Ltd Chromosome-based platforms
DE60236182D1 (de) * 2001-09-14 2010-06-10 Cellectis Zufällige integration von polynukleotide nach in vivo linearisierung
AU2002350039A1 (en) * 2001-10-29 2003-05-12 Renovis, Inc. Method for isolating cell-type specific mrnas
WO2003064604A2 (en) * 2002-01-25 2003-08-07 The Board Of Trustees Of The Leland Stanford Junior University Translation profiling
US20060183147A1 (en) * 2005-02-11 2006-08-17 Elan Pharmaceuticals, Inc. Methods of identifying genes which modulate myelination
EP3369827B1 (en) 2008-03-12 2020-12-23 The Rockefeller University Methods and compositions for translational profiling and molecular phenotyping

Also Published As

Publication number Publication date
EP3369827B1 (en) 2020-12-23
EP2265730A2 (en) 2010-12-29
JP2011512871A (ja) 2011-04-28
EP2265730A4 (en) 2012-03-21
US20180155728A1 (en) 2018-06-07
EP2265730B1 (en) 2018-06-06
WO2009114185A2 (en) 2009-09-17
US9816096B2 (en) 2017-11-14
US10947546B2 (en) 2021-03-16
WO2009114185A3 (en) 2010-01-07
EP3369827A1 (en) 2018-09-05
US20110071049A1 (en) 2011-03-24
US20150082470A1 (en) 2015-03-19

Similar Documents

Publication Publication Date Title
JP5670755B2 (ja) 翻訳プロファイリング及び分子表現型解析のための方法及び組成物
Safaiyan et al. White matter aging drives microglial diversity
Ray et al. Comprehensive identification of mRNA isoforms reveals the diversity of neural cell-surface molecules with roles in retinal development and disease
De Pace et al. Synaptic vesicle precursors and lysosomes are transported by different mechanisms in the axon of mammalian neurons
Darbandi et al. Neonatal Tbr1 dosage controls cortical layer 6 connectivity
Riccomagno et al. The RacGAP β2-Chimaerin selectively mediates axonal pruning in the hippocampus
Cajigas et al. The Evf2 ultraconserved enhancer lncRNA functionally and spatially organizes megabase distant genes in the developing forebrain
Thomas-Jinu et al. Non-nuclear pool of splicing factor SFPQ regulates axonal transcripts required for normal motor development
Igaz et al. Dysregulation of the ALS-associated gene TDP-43 leads to neuronal death and degeneration in mice
Cenik et al. Maternal ribosomes are sufficient for tissue diversification during embryonic development in C. elegans
Hoopfer et al. Genomic analysis of Drosophila neuronal remodeling: a role for the RNA-binding protein Boule as a negative regulator of axon pruning
Bonanomi et al. p190RhoGAP filters competing signals to resolve axon guidance conflicts
Olguin et al. KHSRP loss increases neuronal growth and synaptic transmission and alters memory consolidation through RNA stabilization
Nikolaou et al. Cytoplasmic pool of U1 spliceosome protein SNRNP70 shapes the axonal transcriptome and regulates motor connectivity
Tornini et al. linc-mipep and linc-wrb encode micropeptides that regulate chromatin accessibility in vertebrate-specific neural cells
Kumada et al. Ttyh1, a Ca2+‐binding protein localized to the endoplasmic reticulum, is required for early embryonic development
Dey et al. Loss of paired immunoglobin-like type 2 receptor B gene associated with age-related macular degeneration impairs photoreceptor function in mouse retina
Sajan et al. Novel approaches to studying the genetic basis of cerebellar development
Aerts et al. Altered socio-affective communication and amygdala development in mice with protocadherin10-deficient interneurons
Wynne et al. Suppressive Genetic Interactions Between Haploinsufficient Mitochondrial Genes Encoded in the 22q11. 2 Microdeletion Locus Define Brain and Cardiac Phenotypes
CN118120703B (zh) 一种rb1突变体在神经退行性疾病中的应用
Bartelt et al. Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue
Montalban et al. Translational profiling of mouse dopaminoceptive neurons reveals a role of PGE2 in dorsal striatum
Ruppert The role of MID1, MID2, and PAX6 during neuronal differentiation and migration in the embryonic mouse neocortex
Godoy-Corchuelo et al. FUSDelta14 mutation impairs normal brain development and causes systemic metabolic alterations

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120309

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20120309

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130212

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20131220

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20140314

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20140324

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20140418

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20140425

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140520

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20141128

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20141218

R150 Certificate of patent or registration of utility model

Ref document number: 5670755

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees