JP5618413B2 - 大型核酸のアッセンブリー - Google Patents

大型核酸のアッセンブリー Download PDF

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Publication number
JP5618413B2
JP5618413B2 JP2010528976A JP2010528976A JP5618413B2 JP 5618413 B2 JP5618413 B2 JP 5618413B2 JP 2010528976 A JP2010528976 A JP 2010528976A JP 2010528976 A JP2010528976 A JP 2010528976A JP 5618413 B2 JP5618413 B2 JP 5618413B2
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nucleic acid
fragments
dna
assembled
genome
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Expired - Fee Related
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JP2010528976A
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English (en)
Japanese (ja)
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JP2010539994A5 (OSRAM
JP2010539994A (ja
Inventor
ジー． ギブソン、ダニエル
ジー． ギブソン、ダニエル
エー． ベンダーズ、グウィネズ
エー． ベンダーズ、グウィネズ
ヤング、レイ
アイ． グラス、ジョン
アイ． グラス、ジョン
ベンター、ジェイ．クレイグ
エー． ハッチンソン、Ｉｉｉ、クライド
エー． ハッチンソン、Ｉｉｉ、クライド
オー． スミス、ハミルトン
オー． スミス、ハミルトン
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シンセティック ゲノミクス、インク．
シンセティック ゲノミクス、インク．
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2010528976A 2007-10-08 2008-10-07 大型核酸のアッセンブリー Expired - Fee Related JP5618413B2 (ja)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US97838807P 2007-10-08 2007-10-08
US60/978,388 2007-10-08
US98354907P 2007-10-29 2007-10-29
US60/983,549 2007-10-29
US6221408P 2008-01-23 2008-01-23
US61/062,214 2008-01-23
US2339208P 2008-01-24 2008-01-24
US61/023,392 2008-01-24
US9627008P 2008-09-11 2008-09-11
US61/096,270 2008-09-11
PCT/US2008/079109 WO2009048885A2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Publications (3)

Publication Number Publication Date
JP2010539994A JP2010539994A (ja) 2010-12-24
JP2010539994A5 JP2010539994A5 (OSRAM) 2011-08-25
JP5618413B2 true JP5618413B2 (ja) 2014-11-05

Family

ID=40549815

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010528976A Expired - Fee Related JP5618413B2 (ja) 2007-10-08 2008-10-07 大型核酸のアッセンブリー

Country Status (11)

Country Link
US (1) US9593329B2 (OSRAM)
EP (1) EP2207899B1 (OSRAM)
JP (1) JP5618413B2 (OSRAM)
CN (1) CN101932725A (OSRAM)
AU (1) AU2008311000B2 (OSRAM)
CA (1) CA2701923C (OSRAM)
DK (1) DK2207899T3 (OSRAM)
IL (1) IL204892B (OSRAM)
MX (1) MX2010003644A (OSRAM)
MY (1) MY161418A (OSRAM)
WO (1) WO2009048885A2 (OSRAM)

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US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2010045326A1 (en) 2008-10-14 2010-04-22 Barnes Wayne M In vitro recombination methods using 5' exonuclease
SG171760A1 (en) 2008-11-19 2011-07-28 Amyris Inc Compositions and methods for the assembly of polynucleotides
CA2754212C (en) 2009-03-06 2016-08-02 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US9821052B2 (en) 2009-07-31 2017-11-21 Seqirus UK Limited Reverse genetics systems
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
EP2542676A1 (en) * 2010-03-05 2013-01-09 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US9689012B2 (en) 2010-10-12 2017-06-27 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
CN107058059B (zh) 2011-09-26 2020-08-07 基因技术股份公司 高效的小体积核酸合成
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20140303036A1 (en) * 2011-11-23 2014-10-09 Dsm Ip Assets B.V. Nucleic Acid Assembly System
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
CA2882661C (en) * 2012-08-31 2018-12-11 Synthetic Genomics, Inc. Crowding agent-induced nucleic acid transfer into a recipient host cell
US20140308710A1 (en) * 2012-12-13 2014-10-16 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
CA2898961A1 (en) * 2013-01-23 2014-07-31 Novartis Ag Influenza virus reassortment
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
EP3167071B1 (en) 2014-07-09 2020-10-07 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
CN107532129B (zh) 2014-12-09 2022-09-13 生命技术公司 高效小体积核酸合成
CN106282224B (zh) * 2015-06-26 2020-05-01 深圳华大生命科学研究院 在靶细胞中构建人工染色体的方法以及真核细胞
EP3359669B1 (en) 2015-10-06 2020-05-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
EP3433354B1 (en) 2016-03-23 2023-12-27 Synthetic Genomics, Inc. Generation of synthetic genomes
US20210284991A1 (en) * 2016-11-21 2021-09-16 Novozymes A/S Yeast Cell Extract Assisted Construction of DNA Molecules
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
EP3814494B1 (en) 2018-06-29 2023-11-01 Thermo Fisher Scientific GENEART GmbH High throughput assembly of nucleic acid molecules
CN113056327A (zh) * 2018-07-23 2021-06-29 Dna斯克瑞普特公司 核酸链的大规模平行酶促合成
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
US20220235378A1 (en) * 2019-05-06 2022-07-28 Dsm Ip Assets B.V. Multipartite crispr donor
WO2021178809A1 (en) 2020-03-06 2021-09-10 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN113380322B (zh) * 2021-06-25 2023-10-24 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

Family Cites Families (13)

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US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
AU6487196A (en) 1996-07-09 1998-02-02 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Transformation-associated recombination cloning
WO1999014318A1 (en) * 1997-09-16 1999-03-25 Board Of Regents, The University Of Texas System Method for the complete chemical synthesis and assembly of genes and genomes
US6670154B1 (en) * 1998-07-27 2003-12-30 Ronald A. Hitzeman Automatic eukaryotic artificial chromosome vector
AU5597200A (en) * 1999-06-07 2000-12-28 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
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JP2007534303A (ja) 2003-05-22 2007-11-29 ユニバーシティー オブ カリフォルニア 合成遺伝子または他のｄｎａ配列を作製するための方法
WO2007021944A2 (en) 2005-08-11 2007-02-22 The J. Craig Venter Institute In vitro recombination method
ATE483802T1 (de) 2005-08-11 2010-10-15 Synthetic Genomics Inc Verfahren zur in vitro-rekombination
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Also Published As

Publication number Publication date
DK2207899T3 (en) 2018-09-03
MX2010003644A (es) 2010-06-23
CA2701923A1 (en) 2009-04-16
CA2701923C (en) 2018-07-10
MY161418A (en) 2017-04-14
IL204892B (en) 2018-02-28
AU2008311000A1 (en) 2009-04-16
JP2010539994A (ja) 2010-12-24
EP2207899A4 (en) 2010-12-01
IL204892A0 (en) 2010-11-30
AU2008311000A2 (en) 2010-06-03
AU2008311000B2 (en) 2013-12-19
WO2009048885A3 (en) 2009-12-30
EP2207899B1 (en) 2018-04-18
CN101932725A (zh) 2010-12-29
WO2009048885A2 (en) 2009-04-16
US9593329B2 (en) 2017-03-14
EP2207899A2 (en) 2010-07-21
US20090275086A1 (en) 2009-11-05

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