CA2701923C - Assembly of large nucleic acids - Google Patents
Assembly of large nucleic acids Download PDFInfo
- Publication number
- CA2701923C CA2701923C CA2701923A CA2701923A CA2701923C CA 2701923 C CA2701923 C CA 2701923C CA 2701923 A CA2701923 A CA 2701923A CA 2701923 A CA2701923 A CA 2701923A CA 2701923 C CA2701923 C CA 2701923C
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- dna
- cassettes
- nucleic acid
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- C—CHEMISTRY; METALLURGY
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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| US9234226B2 (en) | 2008-10-14 | 2016-01-12 | Wayne M. Barnes | In vitro recombination methods using 5′ exonuclease |
| AU2009316660B2 (en) | 2008-11-19 | 2015-01-29 | Amrys, Inc. | Compositions and methods for the assembly of polynucleotides |
| DK2403944T3 (da) | 2009-03-06 | 2019-05-27 | Synthetic Genomics Inc | Methods for cloning and manipulating genomes |
| JP2013500712A (ja) | 2009-07-31 | 2013-01-10 | ノバルティス アーゲー | 逆遺伝学系 |
| US20110269119A1 (en) | 2009-10-30 | 2011-11-03 | Synthetic Genomics, Inc. | Encoding text into nucleic acid sequences |
| CN102939380A (zh) * | 2010-03-05 | 2013-02-20 | 合成基因组股份有限公司 | 用于克隆和操作基因组的方法 |
| US9689012B2 (en) | 2010-10-12 | 2017-06-27 | Cornell University | Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements |
| WO2013049227A2 (en) | 2011-09-26 | 2013-04-04 | Geneart Ag | High efficiency, small volume nucleic acid synthesis |
| WO2014153188A2 (en) | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| US20140303036A1 (en) * | 2011-11-23 | 2014-10-09 | Dsm Ip Assets B.V. | Nucleic Acid Assembly System |
| US10829772B2 (en) | 2012-06-22 | 2020-11-10 | Monsanto Technology Llc | Unique modular vector design |
| US9206435B2 (en) * | 2012-08-31 | 2015-12-08 | Synthetic Genomics, Inc. | Crowding agent-induced nucleic acid transfer into a recipient host cell |
| WO2014093535A1 (en) * | 2012-12-13 | 2014-06-19 | Synthetic Genomics, Inc. | Peg-mediated assembly of nucleic acid molecules |
| US20140274806A1 (en) | 2013-03-15 | 2014-09-18 | Synthetic Genomics Vaccines | Influenza virus reassortment |
| JP2016506724A (ja) * | 2013-01-23 | 2016-03-07 | ノバルティス アーゲー | インフルエンザウイルスの再集合 |
| WO2016007604A1 (en) | 2014-07-09 | 2016-01-14 | Gen9, Inc. | Compositions and methods for site-directed dna nicking and cleaving |
| CN115927293A (zh) | 2014-12-09 | 2023-04-07 | 生命技术公司 | 高效小体积核酸合成 |
| CN106282224B (zh) * | 2015-06-26 | 2020-05-01 | 深圳华大生命科学研究院 | 在靶细胞中构建人工染色体的方法以及真核细胞 |
| US20180291413A1 (en) | 2015-10-06 | 2018-10-11 | Thermo Fisher Scientific Geneart Gmbh | Devices and methods for producing nucleic acids and proteins |
| US11085037B2 (en) | 2016-03-23 | 2021-08-10 | Codex Dna, Inc. | Generation of synthetic genomes |
| EP3541954A1 (en) * | 2016-11-21 | 2019-09-25 | Novozymes A/S | Yeast cell extract assisted construction of dna molecules |
| US10726110B2 (en) * | 2017-03-01 | 2020-07-28 | Seven Bridges Genomics, Inc. | Watermarking for data security in bioinformatic sequence analysis |
| WO2020001783A1 (en) | 2018-06-29 | 2020-01-02 | Thermo Fisher Scientific Geneart Gmbh | High throughput assembly of nucleic acid molecules |
| EP3826762A1 (en) | 2018-07-23 | 2021-06-02 | DNA Script | Massively parallel enzymatic synthesis of nucleic acid strands |
| GB201905303D0 (en) | 2019-04-15 | 2019-05-29 | Thermo Fisher Scient Geneart Gmbh | Multiplex assembly of nucleic acid molecules |
| EP3966324A1 (en) * | 2019-05-06 | 2022-03-16 | DSM IP Assets B.V. | Multipartite crispr donor |
| US20240025939A1 (en) | 2020-03-06 | 2024-01-25 | Life Technologies Corporation | High sequence fidelity nucleic acid synthesis and assembly |
| CN113380322B (zh) * | 2021-06-25 | 2023-10-24 | 倍生生物科技(深圳)有限公司 | 人工核酸序列水印编码系统、水印字符串及编码和解码方法 |
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| US5976846A (en) * | 1996-01-13 | 1999-11-02 | Passmore; Steven E. | Method for multifragment in vivo cloning and mutation mapping |
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| JP2007534303A (ja) * | 2003-05-22 | 2007-11-29 | ユニバーシティー オブ カリフォルニア | 合成遺伝子または他のｄｎａ配列を作製するための方法 |
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| DE602006017405D1 (de) | 2005-08-11 | 2010-11-18 | Synthetic Genomics Inc | Verfahren zur in vitro-rekombination |
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| EP2195426B1 (en) | 2007-10-05 | 2013-06-12 | Sapphire Energy, Inc. | System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts |
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| JP2010539994A (ja) | 2010-12-24 |
| IL204892A0 (en) | 2010-11-30 |
| WO2009048885A2 (en) | 2009-04-16 |
| EP2207899A4 (en) | 2010-12-01 |
| WO2009048885A3 (en) | 2009-12-30 |
| AU2008311000B2 (en) | 2013-12-19 |
| AU2008311000A2 (en) | 2010-06-03 |
| EP2207899B1 (en) | 2018-04-18 |
| JP5618413B2 (ja) | 2014-11-05 |
| EP2207899A2 (en) | 2010-07-21 |
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