AU2008311000B2 - Assembly of large nucleic acids - Google Patents

Assembly of large nucleic acids Download PDF

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Publication number
AU2008311000B2
AU2008311000B2 AU2008311000A AU2008311000A AU2008311000B2 AU 2008311000 B2 AU2008311000 B2 AU 2008311000B2 AU 2008311000 A AU2008311000 A AU 2008311000A AU 2008311000 A AU2008311000 A AU 2008311000A AU 2008311000 B2 AU2008311000 B2 AU 2008311000B2
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AU
Australia
Prior art keywords
nucleic acid
fragments
acid molecule
assembled
dna
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Ceased
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AU2008311000A
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English (en)
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AU2008311000A1 (en
AU2008311000A2 (en
Inventor
Gwynedd A. Benders
Daniel G. Gibson
John I. Glass
Clyde A. Hutchison
Hamilton O. Smith
J. Craig Venter
Lei Young
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Viridos Inc
Original Assignee
Synthetic Genomics Inc
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Publication of AU2008311000A1 publication Critical patent/AU2008311000A1/en
Publication of AU2008311000A2 publication Critical patent/AU2008311000A2/en
Application granted granted Critical
Publication of AU2008311000B2 publication Critical patent/AU2008311000B2/en
Ceased legal-status Critical Current
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2008311000A 2007-10-08 2008-10-07 Assembly of large nucleic acids Ceased AU2008311000B2 (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US97838807P 2007-10-08 2007-10-08
US60/978,388 2007-10-08
US98354907P 2007-10-29 2007-10-29
US60/983,549 2007-10-29
US6221408P 2008-01-23 2008-01-23
US61/062,214 2008-01-23
US2339208P 2008-01-24 2008-01-24
US61/023,392 2008-01-24
US9627008P 2008-09-11 2008-09-11
US61/096,270 2008-09-11
PCT/US2008/079109 WO2009048885A2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Publications (3)

Publication Number Publication Date
AU2008311000A1 AU2008311000A1 (en) 2009-04-16
AU2008311000A2 AU2008311000A2 (en) 2010-06-03
AU2008311000B2 true AU2008311000B2 (en) 2013-12-19

Family

ID=40549815

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008311000A Ceased AU2008311000B2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Country Status (11)

Country Link
US (1) US9593329B2 (OSRAM)
EP (1) EP2207899B1 (OSRAM)
JP (1) JP5618413B2 (OSRAM)
CN (1) CN101932725A (OSRAM)
AU (1) AU2008311000B2 (OSRAM)
CA (1) CA2701923C (OSRAM)
DK (1) DK2207899T3 (OSRAM)
IL (1) IL204892B (OSRAM)
MX (1) MX2010003644A (OSRAM)
MY (1) MY161418A (OSRAM)
WO (1) WO2009048885A2 (OSRAM)

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US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US9234226B2 (en) 2008-10-14 2016-01-12 Wayne M. Barnes In vitro recombination methods using 5′ exonuclease
AU2009316660B2 (en) 2008-11-19 2015-01-29 Amrys, Inc. Compositions and methods for the assembly of polynucleotides
DK2403944T3 (da) 2009-03-06 2019-05-27 Synthetic Genomics Inc Methods for cloning and manipulating genomes
JP2013500712A (ja) 2009-07-31 2013-01-10 ノバルティス アーゲー 逆遺伝学系
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
CN102939380A (zh) * 2010-03-05 2013-02-20 合成基因组股份有限公司 用于克隆和操作基因组的方法
US9689012B2 (en) 2010-10-12 2017-06-27 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
WO2013049227A2 (en) 2011-09-26 2013-04-04 Geneart Ag High efficiency, small volume nucleic acid synthesis
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20140303036A1 (en) * 2011-11-23 2014-10-09 Dsm Ip Assets B.V. Nucleic Acid Assembly System
US10829772B2 (en) 2012-06-22 2020-11-10 Monsanto Technology Llc Unique modular vector design
US9206435B2 (en) * 2012-08-31 2015-12-08 Synthetic Genomics, Inc. Crowding agent-induced nucleic acid transfer into a recipient host cell
WO2014093535A1 (en) * 2012-12-13 2014-06-19 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
JP2016506724A (ja) * 2013-01-23 2016-03-07 ノバルティス アーゲー インフルエンザウイルスの再集合
WO2016007604A1 (en) 2014-07-09 2016-01-14 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
CN115927293A (zh) 2014-12-09 2023-04-07 生命技术公司 高效小体积核酸合成
CN106282224B (zh) * 2015-06-26 2020-05-01 深圳华大生命科学研究院 在靶细胞中构建人工染色体的方法以及真核细胞
US20180291413A1 (en) 2015-10-06 2018-10-11 Thermo Fisher Scientific Geneart Gmbh Devices and methods for producing nucleic acids and proteins
US11085037B2 (en) 2016-03-23 2021-08-10 Codex Dna, Inc. Generation of synthetic genomes
EP3541954A1 (en) * 2016-11-21 2019-09-25 Novozymes A/S Yeast cell extract assisted construction of dna molecules
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
WO2020001783A1 (en) 2018-06-29 2020-01-02 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
EP3826762A1 (en) 2018-07-23 2021-06-02 DNA Script Massively parallel enzymatic synthesis of nucleic acid strands
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
EP3966324A1 (en) * 2019-05-06 2022-03-16 DSM IP Assets B.V. Multipartite crispr donor
US20240025939A1 (en) 2020-03-06 2024-01-25 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN113380322B (zh) * 2021-06-25 2023-10-24 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法
EP4638776A1 (en) 2022-12-19 2025-10-29 Thermo Fisher Scientific GENEART GmbH Retrieval of sequence-verified nucleic acid molecules

Citations (1)

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US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping

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US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
JP2000515011A (ja) 1996-07-09 2000-11-14 アメリカ合衆国 形質転換関連組換えクローニング
DE69830065T2 (de) * 1997-09-16 2006-01-19 Egea Biosciences, LLC, San Diego Methoden zur kompletten chemischen synthese und zusammensetzung von genen und genomen
AU5217599A (en) 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
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JP2007534303A (ja) * 2003-05-22 2007-11-29 ユニバーシティー オブ カリフォルニア 合成遺伝子または他のdna配列を作製するための方法
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DE602006017405D1 (de) 2005-08-11 2010-11-18 Synthetic Genomics Inc Verfahren zur in vitro-rekombination
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US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping

Non-Patent Citations (1)

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Also Published As

Publication number Publication date
MY161418A (en) 2017-04-14
DK2207899T3 (en) 2018-09-03
AU2008311000A1 (en) 2009-04-16
US9593329B2 (en) 2017-03-14
US20090275086A1 (en) 2009-11-05
CN101932725A (zh) 2010-12-29
IL204892B (en) 2018-02-28
CA2701923A1 (en) 2009-04-16
MX2010003644A (es) 2010-06-23
JP2010539994A (ja) 2010-12-24
IL204892A0 (en) 2010-11-30
WO2009048885A2 (en) 2009-04-16
EP2207899A4 (en) 2010-12-01
WO2009048885A3 (en) 2009-12-30
AU2008311000A2 (en) 2010-06-03
EP2207899B1 (en) 2018-04-18
CA2701923C (en) 2018-07-10
JP5618413B2 (ja) 2014-11-05
EP2207899A2 (en) 2010-07-21

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