DK2207899T3 - COLLECTION OF LARGE NUCLEIC ACIDS - Google Patents

COLLECTION OF LARGE NUCLEIC ACIDS Download PDF

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Publication number
DK2207899T3
DK2207899T3 DK08838436.7T DK08838436T DK2207899T3 DK 2207899 T3 DK2207899 T3 DK 2207899T3 DK 08838436 T DK08838436 T DK 08838436T DK 2207899 T3 DK2207899 T3 DK 2207899T3
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Prior art keywords
dna
fragments
cassettes
yeast
genome
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DK08838436.7T
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Danish (da)
English (en)
Inventor
J Craig Venter
Daniel G Gibson
Lei Young
John I Glass
Clyde A Hutchison
Hamilton O Smith
Gwynedd A Benders
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Synthetic Genomics Inc
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Publication of DK2207899T3 publication Critical patent/DK2207899T3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK08838436.7T 2007-10-08 2008-10-07 COLLECTION OF LARGE NUCLEIC ACIDS DK2207899T3 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US97838807P 2007-10-08 2007-10-08
US98354907P 2007-10-29 2007-10-29
US6221408P 2008-01-23 2008-01-23
US2339208P 2008-01-24 2008-01-24
US9627008P 2008-09-11 2008-09-11
PCT/US2008/079109 WO2009048885A2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Publications (1)

Publication Number Publication Date
DK2207899T3 true DK2207899T3 (en) 2018-09-03

Family

ID=40549815

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08838436.7T DK2207899T3 (en) 2007-10-08 2008-10-07 COLLECTION OF LARGE NUCLEIC ACIDS

Country Status (11)

Country Link
US (1) US9593329B2 (OSRAM)
EP (1) EP2207899B1 (OSRAM)
JP (1) JP5618413B2 (OSRAM)
CN (1) CN101932725A (OSRAM)
AU (1) AU2008311000B2 (OSRAM)
CA (1) CA2701923C (OSRAM)
DK (1) DK2207899T3 (OSRAM)
IL (1) IL204892B (OSRAM)
MX (1) MX2010003644A (OSRAM)
MY (1) MY161418A (OSRAM)
WO (1) WO2009048885A2 (OSRAM)

Families Citing this family (30)

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US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2010045326A1 (en) 2008-10-14 2010-04-22 Barnes Wayne M In vitro recombination methods using 5' exonuclease
SG171760A1 (en) 2008-11-19 2011-07-28 Amyris Inc Compositions and methods for the assembly of polynucleotides
CA2754212C (en) 2009-03-06 2016-08-02 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US9821052B2 (en) 2009-07-31 2017-11-21 Seqirus UK Limited Reverse genetics systems
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
EP2542676A1 (en) * 2010-03-05 2013-01-09 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
US9689012B2 (en) 2010-10-12 2017-06-27 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
CN107058059B (zh) 2011-09-26 2020-08-07 基因技术股份公司 高效的小体积核酸合成
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20140303036A1 (en) * 2011-11-23 2014-10-09 Dsm Ip Assets B.V. Nucleic Acid Assembly System
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
CA2882661C (en) * 2012-08-31 2018-12-11 Synthetic Genomics, Inc. Crowding agent-induced nucleic acid transfer into a recipient host cell
US20140308710A1 (en) * 2012-12-13 2014-10-16 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
CA2898961A1 (en) * 2013-01-23 2014-07-31 Novartis Ag Influenza virus reassortment
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
EP3167071B1 (en) 2014-07-09 2020-10-07 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
CN107532129B (zh) 2014-12-09 2022-09-13 生命技术公司 高效小体积核酸合成
CN106282224B (zh) * 2015-06-26 2020-05-01 深圳华大生命科学研究院 在靶细胞中构建人工染色体的方法以及真核细胞
EP3359669B1 (en) 2015-10-06 2020-05-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
EP3433354B1 (en) 2016-03-23 2023-12-27 Synthetic Genomics, Inc. Generation of synthetic genomes
US20210284991A1 (en) * 2016-11-21 2021-09-16 Novozymes A/S Yeast Cell Extract Assisted Construction of DNA Molecules
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
EP3814494B1 (en) 2018-06-29 2023-11-01 Thermo Fisher Scientific GENEART GmbH High throughput assembly of nucleic acid molecules
CN113056327A (zh) * 2018-07-23 2021-06-29 Dna斯克瑞普特公司 核酸链的大规模平行酶促合成
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
US20220235378A1 (en) * 2019-05-06 2022-07-28 Dsm Ip Assets B.V. Multipartite crispr donor
WO2021178809A1 (en) 2020-03-06 2021-09-10 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN113380322B (zh) * 2021-06-25 2023-10-24 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

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US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
AU6487196A (en) 1996-07-09 1998-02-02 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Transformation-associated recombination cloning
WO1999014318A1 (en) * 1997-09-16 1999-03-25 Board Of Regents, The University Of Texas System Method for the complete chemical synthesis and assembly of genes and genomes
US6670154B1 (en) * 1998-07-27 2003-12-30 Ronald A. Hitzeman Automatic eukaryotic artificial chromosome vector
AU5597200A (en) * 1999-06-07 2000-12-28 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
US7822969B2 (en) * 2001-04-16 2010-10-26 Digimarc Corporation Watermark systems and methods
AU2002332027A1 (en) 2001-10-04 2003-04-14 The Government Of The United States Of America, As Tandem repeat markers
JP2007534303A (ja) 2003-05-22 2007-11-29 ユニバーシティー オブ カリフォルニア 合成遺伝子または他のdna配列を作製するための方法
WO2007021944A2 (en) 2005-08-11 2007-02-22 The J. Craig Venter Institute In vitro recombination method
ATE483802T1 (de) 2005-08-11 2010-10-15 Synthetic Genomics Inc Verfahren zur in vitro-rekombination
CA2625971A1 (en) 2005-10-12 2007-04-26 The J. Craig Venter Institute Minimal bacterial genome
CA2698627A1 (en) 2007-10-05 2009-04-09 Sapphire Energy, Inc. System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts

Also Published As

Publication number Publication date
MX2010003644A (es) 2010-06-23
CA2701923A1 (en) 2009-04-16
CA2701923C (en) 2018-07-10
JP5618413B2 (ja) 2014-11-05
MY161418A (en) 2017-04-14
IL204892B (en) 2018-02-28
AU2008311000A1 (en) 2009-04-16
JP2010539994A (ja) 2010-12-24
EP2207899A4 (en) 2010-12-01
IL204892A0 (en) 2010-11-30
AU2008311000A2 (en) 2010-06-03
AU2008311000B2 (en) 2013-12-19
WO2009048885A3 (en) 2009-12-30
EP2207899B1 (en) 2018-04-18
CN101932725A (zh) 2010-12-29
WO2009048885A2 (en) 2009-04-16
US9593329B2 (en) 2017-03-14
EP2207899A2 (en) 2010-07-21
US20090275086A1 (en) 2009-11-05

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