JP5575239B2 - 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物 - Google Patents

拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物 Download PDF

Info

Publication number
JP5575239B2
JP5575239B2 JP2012521757A JP2012521757A JP5575239B2 JP 5575239 B2 JP5575239 B2 JP 5575239B2 JP 2012521757 A JP2012521757 A JP 2012521757A JP 2012521757 A JP2012521757 A JP 2012521757A JP 5575239 B2 JP5575239 B2 JP 5575239B2
Authority
JP
Japan
Prior art keywords
amplicon
amplification
nucleic acid
probes
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2012521757A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013500013A (ja
Inventor
ケネス エー． ブラウン，
ダニエル エル． カシアン，
Original Assignee
ジェン−プローブ・インコーポレーテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ジェン−プローブ・インコーポレーテッド filed Critical ジェン−プローブ・インコーポレーテッド
Publication of JP2013500013A publication Critical patent/JP2013500013A/ja
Application granted granted Critical
Publication of JP5575239B2 publication Critical patent/JP5575239B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2012521757A 2009-07-21 2010-07-21 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物 Expired - Fee Related JP5575239B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US22733909P 2009-07-21 2009-07-21
US61/227,339 2009-07-21
US23093809P 2009-08-03 2009-08-03
US61/230,938 2009-08-03
PCT/US2010/042776 WO2011011535A2 (en) 2009-07-21 2010-07-21 Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2013235628A Division JP5863752B2 (ja) 2009-07-21 2013-11-14 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物

Publications (2)

Publication Number Publication Date
JP2013500013A JP2013500013A (ja) 2013-01-07
JP5575239B2 true JP5575239B2 (ja) 2014-08-20

Family

ID=43084221

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2012521757A Expired - Fee Related JP5575239B2 (ja) 2009-07-21 2010-07-21 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物
JP2013235628A Expired - Fee Related JP5863752B2 (ja) 2009-07-21 2013-11-14 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物
JP2015143645A Withdrawn JP2015186487A (ja) 2009-07-21 2015-07-21 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物
JP2017114116A Pending JP2017148080A (ja) 2009-07-21 2017-06-09 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2013235628A Expired - Fee Related JP5863752B2 (ja) 2009-07-21 2013-11-14 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物
JP2015143645A Withdrawn JP2015186487A (ja) 2009-07-21 2015-07-21 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物
JP2017114116A Pending JP2017148080A (ja) 2009-07-21 2017-06-09 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物

Country Status (6)

Country Link
US (5) US8628924B2 (cg-RX-API-DMAC7.html)
EP (3) EP3018218B1 (cg-RX-API-DMAC7.html)
JP (4) JP5575239B2 (cg-RX-API-DMAC7.html)
AU (1) AU2010276236B2 (cg-RX-API-DMAC7.html)
CA (1) CA2768391C (cg-RX-API-DMAC7.html)
WO (1) WO2011011535A2 (cg-RX-API-DMAC7.html)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2545189B1 (en) 2010-03-08 2018-01-10 Dana-Farber Cancer Institute, Inc. Full cold-pcr enrichment with reference blocking sequence
US11130992B2 (en) 2011-03-31 2021-09-28 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex COLD-PCR
US9133490B2 (en) * 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
EP2749654A1 (en) * 2012-12-28 2014-07-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method of analysis of composition of nucleic acid mixtures
JP6491458B2 (ja) * 2014-10-31 2019-03-27 シスメックス株式会社 核酸増幅方法
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
JP2020525459A (ja) 2017-06-26 2020-08-27 アンスティテュ・パストゥール Ｈｉｖリザーバーを排除し、ウイルス負荷を低減する処置
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
WO2019070731A1 (en) * 2017-10-03 2019-04-11 Abbott Molecular Inc. ASSAY FOR DETECTION OF HUMAN IMMUNODEFICIENCY VIRUS (HIV)

Family Cites Families (107)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4486530A (en) 1980-08-04 1984-12-04 Hybritech Incorporated Immunometric assays using monoclonal antibodies
US4514505A (en) 1982-05-21 1985-04-30 The Trustees Of Columbia University In The City Of New York Monoclonal antibody mixtures and use thereof for enhanced sensitivity immunoassays
ATE202801T1 (de) 1983-01-10 2001-07-15 Gen Probe Inc Verfahren zum nachweis, identifizieren und quantifizieren von organismen und viren
US4595661A (en) 1983-11-18 1986-06-17 Beckman Instruments, Inc. Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect
US4851334A (en) 1984-01-03 1989-07-25 The New York Blood Center, Inc. Monoclonal antibodies specific to in vivo fragments derived from human fibrinogen, human fibrin I or human fibrin II
US4766062A (en) 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US6040166A (en) 1985-03-28 2000-03-21 Roche Molecular Systems, Inc. Kits for amplifying and detecting nucleic acid sequences, including a probe
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US6197563B1 (en) 1985-03-28 2001-03-06 Roche Molecular Systems, Inc. Kits for amplifying and detecting nucleic acid sequences
US5616729A (en) 1986-07-17 1997-04-01 Board Of Governors Of Wayne State University Enhanced chemiluminescence from 1,2-dioxetanes through energy transfer to tethered fluorescers
US5004565A (en) 1986-07-17 1991-04-02 The Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
US5707559A (en) 1986-07-17 1998-01-13 Tropix, Inc. Chemiluminescent 1,2-dioxetane compounds
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US4745181A (en) 1986-10-06 1988-05-17 Ciba Corning Diagnostics Corp. Polysubstituted aryl acridinium esters
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US5283174A (en) 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
US5185439A (en) 1987-10-05 1993-02-09 Gen-Probe Incorporated Acridinium ester labelling and purification of nucleotide probes
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
GB2233450B (en) 1989-06-24 1993-06-30 Univ Wales Medicine Detecting or quantifing multiple analytes with luminescent reagents
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5219727A (en) 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5516663A (en) 1990-01-26 1996-05-14 Abbott Laboratories Ligase chain reaction with endonuclease IV correction and contamination control
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5312921A (en) 1990-03-14 1994-05-17 Regents Of The University Of California Dyes designed for high sensitivity detection of double-stranded DNA
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
AU687363B2 (en) 1993-03-19 1998-02-26 Ciba Corning Diagnostics Corp. Luminometer
US5395752A (en) 1993-03-19 1995-03-07 Ciba Corning Diagnostics Corp. Long emission wavelength chemiluminescent compounds and their use in test assays
US5876978A (en) 1993-04-06 1999-03-02 Medical College Of Ohio Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
JP3026843B2 (ja) 1993-07-23 2000-03-27 ジェン−プローブ・インコーポレーテッド 核酸増幅の促進法
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
GB9326238D0 (en) 1993-12-23 1994-02-23 Sinvent As Method of assay
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5547861A (en) 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US5514551A (en) 1994-10-14 1996-05-07 Gen-Probe Incorporated Compositions for the detection of Chlamydia trachomatis
ES2271944T3 (es) 1994-10-28 2007-04-16 Gen-Probe Incorporated Composiciones y metodos para la deteccion y cuantificacion simultaneas de multiples secuencias especificas de acidos nucleicos.
JP3189000B2 (ja) 1994-12-01 2001-07-16 東ソー株式会社 特定核酸配列の検出方法
US5830645A (en) 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5514505A (en) * 1995-05-15 1996-05-07 Xerox Corporation Method for obtaining improved image contrast in migration imaging members
DE69620596T2 (de) 1995-05-19 2002-11-21 Abbott Laboratories, Abbott Park Nachweis von nukleinsäuren mit breitem dynamikbereich unter verwendung von aggregat-primer-reihen
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5741650A (en) 1996-01-30 1998-04-21 Exact Laboratories, Inc. Methods for detecting colon cancer from stool samples
ATE318327T1 (de) 1996-06-04 2006-03-15 Univ Utah Res Found Fluoreszenz-donor-akzeptor paar
US20020042048A1 (en) 1997-01-16 2002-04-11 Radoje Drmanac Methods and compositions for detection or quantification of nucleic acid species
JP3968810B2 (ja) 1997-01-24 2007-08-29 東ソー株式会社 核酸配列分析方法
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6165800A (en) 1997-05-30 2000-12-26 Bayer Corporation Chemiluminescent energy transfer conjugates and their uses as labels in binding assays
WO1998056954A1 (en) 1997-06-13 1998-12-17 Affymetrix, Inc. Method to detect gene polymorphisms and monitor allelic expression employing a probe array
US6566101B1 (en) 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
AU1366299A (en) 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
EP1032705B1 (en) 1997-10-30 2011-12-14 Cold Spring Harbor Laboratory Probe arrays and methods of using probe arrays for distinguishing dna
US6180340B1 (en) 1997-10-31 2001-01-30 Gen-Probe Incorporated Extended dynamic range assays
US5989823A (en) 1998-09-18 1999-11-23 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
EP1049803A4 (en) 1997-12-15 2002-08-21 Nexstar Pharmaceuticals Inc HOMOGENEOUS DETECTION OF TARGET MOLECULES BY LIGAND NUCLEIC ACID AND LIGAND BEACON INTERACTION
US6238869B1 (en) 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
AU3979099A (en) 1998-05-11 1999-11-29 Philadelphia Health & Education Corporation (mct-1), a human oncogene
US6066458A (en) 1998-05-18 2000-05-23 Becton, Dickinson And Company Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples using standard curves and amplification ratio estimates
ATE440963T1 (de) 1998-07-02 2009-09-15 Gen Probe Inc Molekulare fackeln
US6037130A (en) 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US20020001800A1 (en) 1998-08-14 2002-01-03 Stanley N. Lapidus Diagnostic methods using serial testing of polymorphic loci
US6245517B1 (en) 1998-09-29 2001-06-12 The United States Of America As Represented By The Department Of Health And Human Services Ratio-based decisions and the quantitative analysis of cDNA micro-array images
WO2000020643A1 (en) 1998-10-05 2000-04-13 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
WO2000034521A1 (en) 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
CA2358699A1 (en) 1999-01-25 2000-07-27 Ut-Battelle, Llc Advanced multifunctional/multispectral biosensor devices and methods of use
US6245511B1 (en) 1999-02-22 2001-06-12 Vialogy Corp Method and apparatus for exponentially convergent therapy effectiveness monitoring using DNA microarray based viral load measurements
EP1230383A2 (en) 1999-04-08 2002-08-14 Sir Mortimer B. Davis Jewish General Hospital Quantitative assay for expression of genes in microarray
US6235479B1 (en) 1999-04-13 2001-05-22 Bio Merieux, Inc. Methods and devices for performing analysis of a nucleic acid sample
DE60045059D1 (de) 1999-04-20 2010-11-18 Nat Inst Of Advanced Ind Scien Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten
AU775563B2 (en) 1999-05-14 2004-08-05 Brandeis University Nucleic acid-based detection
DE60014762T2 (de) 1999-05-24 2005-10-13 Tosoh Corp., Shinnanyo Methode zum Nachweis von Ribonukleinsäuren
AU5733900A (en) 1999-06-09 2000-12-28 Biosearch Technologies, Inc. Fluorescence energy transfer probes with stabilized conformations
US6294338B1 (en) 1999-07-23 2001-09-25 Gen-Probe Incorporated Polynucleotide amplification method
JP4919568B2 (ja) 1999-10-22 2012-04-18 ピーエイチアールアイ プロパティーズ，インコーポレイテッド 短い配列の変異体を検出するためのアッセイ
EP1096024A1 (en) 1999-10-28 2001-05-02 Remacle, José Method and kit for the screening and/or the quantification of multiple homologous nucleic acid sequences on arrays
GB2359625B (en) 1999-12-10 2004-10-20 Molecular Light Tech Res Ltd Monitoring oligonucleotide binding process using chemiluminescence quenching
US20040121364A1 (en) * 2000-02-07 2004-06-24 Mark Chee Multiplex nucleic acid reactions
DE10045521A1 (de) 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen
JP2003535599A (ja) 2000-06-06 2003-12-02 ティーエム バイオサイエンス コーポレイション 核酸の捕捉部とその使用
CA2383939C (en) 2000-06-27 2009-12-01 National Institute Of Advanced Industrial Science And Technology Novel nucleic acid probes, method for determining nucleic acids by using the probes, and method for analyzing data obtained by the method
DE60230787D1 (de) 2001-08-31 2009-02-26 Gen Probe Inc Sonden mit affinitätsverschiebung zur quantifizierung von analytenpolynukleotiden
CA2468713A1 (en) 2001-11-30 2003-06-12 University Of Rochester Multiplex real-time quantitative pcr
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
EP2292801B1 (en) 2002-06-14 2018-08-08 Gen-Probe Incorporated Compositions and methods for detecting hepatitis b virus
DE60324810D1 (de) 2002-09-20 2009-01-02 New England Biolabs Inc HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
WO2004102149A2 (en) * 2003-04-11 2004-11-25 Stratagene California Methods and compositions for the detection of bacterial species
GB0320236D0 (en) 2003-08-29 2003-10-01 Molecular Light Tech Res Ltd Chemiluminescent compounds
WO2006004949A1 (en) 2004-06-30 2006-01-12 Applera Corporation Compositions and methods for detecting and quantitating polynucleotide sequences
EP1776482A2 (en) 2004-06-30 2007-04-25 Applera Corporation Log-linear amplification
CA2980050C (en) 2004-08-27 2018-01-23 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
CN101068937A (zh) * 2004-09-20 2007-11-07 匹兹堡大学 多重模式复合反应测序方法
JP2008517606A (ja) * 2004-10-22 2008-05-29 プロメガ コーポレイション 変異検出のための方法及びキット
JP2008533974A (ja) 2005-01-21 2008-08-28 サード・ウェーブ・テクノロジーズ・インク 核酸分子の増大したダイナミックレンジ検出のための組成物および方法
DE102006007778B3 (de) 2006-02-20 2007-11-08 Biotype Ag Verfahren zur Analyse von Multiplex-Gemischen bestehend aus Nukleinsäure-Amplifikationsprodukten mit überlappenden Detektionsbereichen
US20080131875A1 (en) 2006-06-07 2008-06-05 Third Wave Technologies, Inc. Multiplex assays
US20090181389A1 (en) 2008-01-11 2009-07-16 Signosis, Inc., A California Corporation Quantitative measurement of nucleic acid via ligation-based linear amplification

Also Published As

Publication number Publication date
AU2010276236B2 (en) 2014-03-20
US20140072974A1 (en) 2014-03-13
US8628924B2 (en) 2014-01-14
US9856527B2 (en) 2018-01-02
JP2013500013A (ja) 2013-01-07
EP2456887B1 (en) 2015-11-25
JP2014030431A (ja) 2014-02-20
US8932817B2 (en) 2015-01-13
US20150093753A1 (en) 2015-04-02
US20110020824A1 (en) 2011-01-27
JP5863752B2 (ja) 2016-02-17
EP3330385A1 (en) 2018-06-06
US9347098B2 (en) 2016-05-24
EP3018218A1 (en) 2016-05-11
JP2015186487A (ja) 2015-10-29
CA2768391C (en) 2016-09-06
US20160258009A1 (en) 2016-09-08
AU2010276236A1 (en) 2012-02-16
CA2768391A1 (en) 2011-01-27
US20180216172A1 (en) 2018-08-02
EP2456887A2 (en) 2012-05-30
EP3018218B1 (en) 2018-01-24
WO2011011535A2 (en) 2011-01-27
WO2011011535A3 (en) 2011-04-14
JP2017148080A (ja) 2017-08-31

Similar Documents

Publication Publication Date Title
JP5863752B2 (ja) 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物
US5795722A (en) Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
KR100431421B1 (ko) 형광표지된올리고뉴클레오타이드프로브의용액중에서의켄칭방법
US20030157499A1 (en) Method of assessing the amount of nucleic acid in a sample
JP4559074B2 (ja) 複数分析物の測定方法
JPH11506013A (ja) 生成物の増幅後レベルから核酸標的配列の増幅前レベルを決定するための方法とキット
JP6181742B2 (ja) Ｈｅｖアッセイ
EP1426448A1 (en) Method for lowering the effects of sequence variations in a diagnostic hybridization assay, probe for use in the assay and assay
US20230392198A1 (en) Method and system for detecting reverse transcriptase activity by digital assay
JP4913042B2 (ja) Ｈｉｖタイプおよびサブタイプの検出
AU2018214081A1 (en) Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
AU2014203059A1 (en) Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
Xiao et al. Molecular diagnosis of HIV and relevant novel technologies in mutation analysis
US20040202998A1 (en) Method of amplifying or detecting HIV-1 RNA
JP2024536115A (ja) 核酸増幅生成物を試験する方法
CN117625768A (zh) 一种通用数字pcr检测体系及其应用
JP2001046080A (ja) アンプリコンの製法及びそれを用いた検出法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130123

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20131001

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20131114

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20131114

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20140605

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20140701

R150 Certificate of patent or registration of utility model

Ref document number: 5575239

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees