JP5563374B2 - Animal feed - Google Patents

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JP5563374B2
JP5563374B2 JP2010123413A JP2010123413A JP5563374B2 JP 5563374 B2 JP5563374 B2 JP 5563374B2 JP 2010123413 A JP2010123413 A JP 2010123413A JP 2010123413 A JP2010123413 A JP 2010123413A JP 5563374 B2 JP5563374 B2 JP 5563374B2
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あい子 城田
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
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    • Y02P60/87Re-use of by-products of food processing for fodder production

Description

本発明は、例えば鶏などの家禽、その他の家畜、ペット、養魚などに用いられる、りんごポリフェノールを含む動物用飼料に関する。 The present invention is, for example, poultry such as chickens, and other livestock, pets, used like fish relates to animal fodder containing apple polyphenols.

従来、この種の動物用飼料として、リンゴの果実若しくは未熟果実由来の果実ポリフェノールが有効成分として含有してなる脂肪蓄積抑制剤を、動物用飼料に含有させて用いるものがある(例えば、特許文献1参照)。
このポリフェノールは、果実若しくは未熟果実から抽出・精製されたポリフェノール画分からなり、当該ポリフェノール画分は、まず原料果実の搾汁果汁もしくは水やエタノールなどの有機溶媒を用いて抽出した抽出液を用い、遠心分離やろ過等の工程を経て、清澄な果汁若しくは抽出液が得られ、当該ポリフェノール画分はさらに、イオン交換樹脂や合成吸着樹脂、或いはシリカゲル等の吸着剤やゲルろ過剤等によって精製され、次いで濃縮することにより液体製剤が得られ、さらに濃縮液を噴霧乾燥もしくは凍結乾燥処理することにより粉末製剤を得ている。
その製造例としては、リンゴ幼果を破砕、圧搾して果汁を得た後、遠心分離し、珪藻土濾過により清澄化を行い、この清澄果汁を吸着力ラムに通液し、ポリフェノール類を吸着させ、続いて純水を通液し、カラム中の非吸着物質を除去した後、アルコールで溶出し、得られた画分からアルコールを減圧濃縮し、抽出粉末品を調製している。
Conventionally, as this type of animal feed, there is one that uses a fat accumulation inhibitor containing, as an active ingredient, a fruit polyphenol derived from an apple fruit or an immature fruit (for example, a patent document). 1).
This polyphenol is composed of a polyphenol fraction extracted and purified from fruits or immature fruits, and the polyphenol fraction is first extracted from the juice of the raw fruit or an extract extracted using an organic solvent such as water or ethanol, Through a process such as centrifugation or filtration, a clear fruit juice or extract is obtained, and the polyphenol fraction is further purified by an ion exchange resin, a synthetic adsorption resin, or an adsorbent such as silica gel, a gel filter, or the like, Subsequently, a liquid preparation is obtained by concentration, and a powder preparation is obtained by spray drying or freeze-drying the concentrated liquid.
As an example of production, fruit juice is obtained by crushing and squeezing apple fruit, then centrifuging and clarified by diatomaceous earth filtration, and this clarified fruit juice is passed through an adsorption force ram to adsorb polyphenols. Subsequently, pure water is passed through to remove non-adsorbed substances in the column and then eluted with alcohol, and the alcohol is concentrated under reduced pressure from the obtained fraction to prepare an extracted powder product.

国際公開第2005/082390号パンフレットInternational Publication No. 2005/082390 Pamphlet

しかし乍ら、このような従来の動物用飼料では、リンゴの未熟果実から抽出・精製されるポリフェノール画分を含有させて用いるため、該ポリフェノール画分の製造に手間がかかって、製造コストが高くなり、そのため、多くの動物に対し飼料を長期に亘って十分に与えることが難しく、現実的でなかった。
このような理由もあって、現在までりんご未熟果実のポリフェノールによる薬効が認められた動物用飼料は実際に販売されていない。
However, in such conventional animal feed, since the polyphenol fraction extracted and purified from the immature apple fruit is used, the production of the polyphenol fraction takes time and the production cost is high. Therefore, it has been difficult and unrealistic to provide a sufficient amount of feed for many animals over a long period of time.
For these reasons, animal feeds that have been found to have medicinal effects due to polyphenols of immature apple fruits have not been sold.

本発明は、このような問題に対処することを課題とするものであり、りんごポリフェノールが含まれて動物の成育に安全な動物用飼料を容易に得ること、動物の飼育目的に合わせて必要な栄養分を与えること、などを目的とするものである。 An object of the present invention is to cope with such problems, and it is necessary to easily obtain animal feed that contains apple polyphenols and is safe for animal growth, and is necessary for animal breeding purposes. giving nutrients,, etc. it is an object of the present invention.

このような目的を達成するために本発明は、飼料材料に対し、その重量比でりんご未熟果実の残渣として、未熟リンゴから果汁を抽出した後の搾りかすとなる外皮部位及びその周辺部位をそのまま何も加工せずに5〜50%混合したことを特徴とする。
前述した特徴に加えて、前記飼料材料が配合飼料であることを特徴とする。
さらに、前記配合飼料に対し、その重量比でりんご未熟果実の残渣が8〜18%混合したことを特徴とする
In order to achieve such an object, the present invention provides, as a feed material, as a residue of unripe apple fruit at the weight ratio, the skin part and its peripheral part that will be squeezed after extracting fruit juice from an immature apple as it is. It is characterized by mixing 5 to 50% without any processing.
In addition to the features described above, the feed material is a mixed feed.
Furthermore, it is characterized in that 8 to 18% of apple immature fruit residue is mixed in the weight ratio with the blended feed .

前述した特徴を有する本発明は、飼料材料に対し、その重量比でりんご未熟果実の残渣として、未熟リンゴから果汁を抽出した後の搾りかすとなる外皮部位及びその周辺部位そのまま何も加工せずに5〜50%混合した動物用飼料とすることにより、該動物用飼料が与えられた動物の体内でりんごポリフェノールが生物濃縮されると同時に、飼料材料に含まれる栄養分が不足して動物が成育不良となることもないため、りんごポリフェノールが含まれて動物の成育に安全な動物用飼料を容易に得ることができる。
その結果、リンゴの未熟果実から抽出・精製されるポリフェノール画分を含有させて用いる従来の動物用飼料に比べ、製造に手間がかからず低コストで製造できるため、多くの動物に対し長期に亘って与えることができ、それにより多くの動物がりんごポリフェノールによる薬効を得ることが期待できる。
さらに、未熟リンゴから果汁を抽出した後の搾りかすである残渣を回収利用すれば、産業廃棄物を有効利用することができる。
The present invention having the above-described features allows the feed material to be processed as it is as the residue of unripe apple fruit by weight ratio, and the outer skin part and its peripheral part that will be squeezed after extracting fruit juice from unripe apples. By adding 5 to 50% of the animal feed, the apple polyphenol is bioconcentrated in the body of the animal to which the animal feed is given, and at the same time, the animal lacks the nutrients contained in the feed material. Since it does not cause growth failure, an animal feed that contains apple polyphenols and is safe for animal growth can be easily obtained.
As a result, compared to conventional animal feeds that contain a polyphenol fraction extracted and purified from immature apple fruit, it can be manufactured at low cost without much time for production. Many animals can be expected to obtain the medicinal effects of apple polyphenols.
Furthermore, industrial waste can be effectively used by recovering and using the residue that is the pomace after extracting fruit juice from immature apples.

さらに、前記飼料材料が配合飼料である場合には、動物の飼育目的に合わせて必要な栄養分を与えることができる。   Furthermore, when the said feed material is a mixing | blending feed, a required nutrient can be given according to the breeding purpose of an animal.

さらに、前記飼料材料に対し、その重量比で前記りんご未熟果実の残渣を8〜18%混合した場合には、りんご未熟果実の残渣の添加量が比較的少ない動物用飼料で動物を育成したことにかかわらず、その動物の体内で多量のりんごポリフェノールを生物濃縮させることができる。   Further, when 8 to 18% of the apple immature fruit residue is mixed in the weight ratio with respect to the feed material, the animal is bred with an animal feed with a relatively small amount of apple immature fruit residue added. Regardless, large amounts of apple polyphenols can be bioconcentrated in the animal's body.

また、前記動物用飼料が鶏に与えられた場合には、該鶏の体内でりんごポリフェノールが生物濃縮されてりんごポリフェノール入りの卵が産み出されるため、りんごポリフェノールが含まれた鶏卵を容易に得ることができる。
その結果、このりんごポリフェノールが含まれた鶏卵を人間が食べると、りんごポリフェノールによる薬効が期待できる。
もともと鶏卵は他の食物に比べて安価で長期間に亘り継続して食することが容易であるため、りんごポリフェノールによる薬効も十分に発揮できる。
特に、前記配合飼料に対し、その重量比でりんご未熟果実の残渣が8〜18%、好ましくは10%混合された動物用飼料を与えた場合には、りんご未熟果実の残渣の添加量が比較的少ない動物用飼料で育成しても、多量のりんごポリフェノールが含まれる卵を多く産卵させることができる。
In addition, when the animal feed is given to a chicken, apple polyphenol is bioconcentrated in the chicken's body to produce an egg containing apple polyphenol, so that an egg containing apple polyphenol is easily obtained. be able to.
As a result, when a human eats chicken eggs containing this apple polyphenol, the medicinal effect of apple polyphenol can be expected.
Originally, hen's eggs are cheaper than other foods and can be eaten continuously for a long period of time.
In particular, when an animal feed in which 8 to 18%, preferably 10%, of an apple immature fruit residue is given in a weight ratio to the above-mentioned mixed feed, the amount of added apple immature fruit residue is compared. Even if it grows with a small amount of animal feed, many eggs containing a large amount of apple polyphenol can be laid.

また、前記動物用飼料が鶏に与えられた場合には、該鶏の体内でりんごポリフェノールが生物濃縮されて鶏の肉にりんごポリフェノールが含まれるため、りんごポリフェノールが含まれた鶏肉を容易に得ることができる。
その結果、このりんごポリフェノールが含まれた鶏肉を人間が食べると、りんごポリフェノールによる薬効が期待できる。
In addition, when the animal feed is given to a chicken, apple polyphenol is bioconcentrated in the chicken body and the chicken meat contains the apple polyphenol, so that the chicken meat containing the apple polyphenol can be easily obtained. be able to.
As a result, when a human eats chicken containing this apple polyphenol, the medicinal effect of apple polyphenol can be expected.

またさらに、前記動物用飼料が鶏に与えられた場合には、該鶏の体内でりんごポリフェノールが生物濃縮されてポリフェノール入りの糞が排出されるため、りんごポリフェノールが含まれた鶏糞を容易に得ることができる。
その結果、このりんごポリフェノールが含まれた鶏糞を肥料として植物栽培に用いれば、りんごポリフェノールがより多く含まれる野菜や果実などを生産することができる。
これらの野菜や果実を多種類生産すれば、年間を通し継続して食することが可能となるため、りんごポリフェノールによる薬効も十分に期待できる。
Still further, when the animal feed is given to chickens, apple polyphenols are bioconcentrated in the chicken body and the feces containing polyphenols are discharged, so that chicken feces containing apple polyphenols can be easily obtained. be able to.
As a result, if the chicken manure containing this apple polyphenol is used as a fertilizer for plant cultivation, vegetables, fruits and the like containing more apple polyphenol can be produced.
If many kinds of these vegetables and fruits are produced, it becomes possible to eat continuously throughout the year, so that the medicinal effects of apple polyphenols can be sufficiently expected.

本発明の実施形態に係る動物用飼料を与えた鶏の産卵個数を示すグラフであり、飼料材料に含まれる未熟リンゴ搾りかすの含有量と鶏の平均産卵個数との関係を示している。It is a graph which shows the egg-laying number of the chicken which gave the animal feed which concerns on embodiment of this invention, and has shown the relationship between content of the immature apple pomace contained in feed material, and the average egg-laying number of a chicken. 本発明の実施形態に係る動物用飼料を与えた鶏卵の総ポリフェノール量を示すグラフであり、飼料材料に含まれる未熟リンゴ搾りかすの含有量と卵中の総ポリフェノール量との関係を示している。It is a graph which shows the total polyphenol amount of the chicken egg which gave the animal feed which concerns on embodiment of this invention, and has shown the relationship between content of the immature apple pomace contained in feed material, and the total polyphenol amount in an egg .

以下、本発明の実施形態を詳細に説明する。
本発明の実施形態に係る動物用飼料は、図1〜図2に示すように、飼料材料に対し、その重量比でりんご未熟果実の残渣を5〜50%混合している。
Hereinafter, embodiments of the present invention will be described in detail.
As shown in FIGS. 1 to 2, the animal feed according to the embodiment of the present invention is mixed with 5 to 50% of apple immature fruit residues in the weight ratio of the feed material.

飼料材料は、1種類のみの材料で作られている単体飼料、2種類以上の単体飼料を混合して作られる混合飼料、2種類以上の飼料を対象の動物によって適正な割合に混合して作られる配合飼料などからなる。   The feed material is made by mixing a single feed made of only one kind of material, a mixed feed made by mixing two or more kinds of simple feeds, and mixing two or more kinds of feeds in an appropriate ratio depending on the target animal. It consists of a blended feed.

りんご未熟果実は、りんご Malus domestica 及び クラブアップル Malus pumila などの Malus 属果実の未熟果実であり、結実後1〜2ケ月に所謂「みすぐり」の過程で、大きな果実を収穫するために半分以上が摘果され、捨てられるものである。
この未熟果実は、害虫や病害が発生する前に収穫するため、殺虫剤や殺菌剤などの農薬の散布を行なわなくても収穫できることになり、このことは、農薬に要した経費が削減できるのみならず、農薬を使用しない有機な果実である。
そして、これらの未熟果実には、成熟果実に比べ単位重量当たり約10倍のりんごポリフェノールが含まれる。
Unripe apples are immature fruits of the genus Malus, such as apples Malus domestica and crab apple Malus pumila, and more than half of them are harvested in the so-called “Tickling” process in 1 to 2 months after fruiting. It is picked and thrown away.
This immature fruit is harvested before the occurrence of pests and diseases, so it can be harvested without spraying pesticides such as insecticides and fungicides, which only reduces the cost of pesticides. It is an organic fruit that does not use pesticides.
These immature fruits contain about 10 times more apple polyphenol per unit weight than mature fruits.

本発明では、りんご未熟果実の残渣、すなわち、未熟リンゴから果汁を抽出した後の搾りかすとなる、主に外皮部位やその周辺部位などを使う。
その具体例としては、未熟リンゴから果汁を抽出した後の搾りかすを回収して、そのまま何も加工をせずに利用することが好ましい。
In the present invention, an apple immature fruit residue, that is, an outer skin part or its peripheral part, which becomes a pomace after extracting fruit juice from an immature apple, is used.
As a specific example, it is preferable to recover the pomace after extracting the fruit juice from the immature apple and use it without any processing.

このような動物用飼料を例えば鶏などの家禽やその他の家畜やペットや養魚などの動物に与えると、飼料材料に対しその重量比でりんご未熟果実の残渣が5〜50%混合される動物用飼料の場合には、該動物の体内でりんごポリフェノールが生物濃縮されることを、以下の実施例で示されるように実証できた。
また、飼料材料に対しその重量比でりんご未熟果実の残渣が5%未満混合された動物用飼料の場合には、飼料材料に対するりんご未熟果実の残渣の混合量が少なくなるため、鶏の体内で生物濃縮されるりんごポリフェノールの量が減少することを、以下の実施例で示されるように実証できた。
さらに、飼料材料に対しその重量比でりんご未熟果実の残渣が50%よりも多く混合された動物用飼料の場合には、飼料材料に含まれる栄養分が不足して動物の育成に悪影響を与えるおそれがある。
これに対して、飼料材料に対しその重量比でりんご未熟果実の残渣を5〜50%混合した動物用飼料を動物に与えると、該動物は重量比で50%〜95%の飼料材料に含まれる栄養分が摂取可能となるため、栄養不足により動物が成育不良となることもない。
それにより、りんごポリフェノールが含まれて動物の成育に安全な動物用飼料を容易に得ることができる。
次に本発明の一実施例について説明する。
For example, when such animal feed is given to poultry such as chickens, other livestock, pets, fish farms, or other animals, the residue of unripe apple fruits is mixed in an amount of 5 to 50% by weight with respect to the feed material. In the case of feed, it has been demonstrated that apple polyphenols are bio-concentrated in the animal's body, as shown in the examples below.
In addition, in the case of animal feed in which less than 5% of apple immature fruit residue is mixed in the weight ratio with respect to the feed material, the mixing amount of apple immature fruit residue relative to the feed material is reduced. A decrease in the amount of bio-enriched apple polyphenols could be demonstrated as shown in the examples below.
Furthermore, in the case of animal feed in which more than 50% of apple immature fruit residue is mixed in the weight ratio with respect to the feed material, nutrients contained in the feed material may be insufficient and adversely affect animal growth. There is.
On the other hand, when the animal feed is mixed with 5 to 50% of an immature apple residue in the weight ratio of the feed material, the animal is included in the feed material of 50% to 95% by weight. This makes it possible to ingest the nutrients that the animal does not suffer from poor growth due to lack of nutrition.
Thereby, an animal feed that contains apple polyphenol and is safe for animal growth can be easily obtained.
Next, an embodiment of the present invention will be described.

この実施例は、飼料材料として鶏用の配合飼料を用い、それにりんご未熟果実の残渣として、未熟リンゴから果汁を抽出した後の搾りかすを、重量比で0%、5%、10%、20%、50%それぞれ混合したものを用意し、夫々の動物用飼料を同条件で鶏20羽ずつに与え、各区の鶏の産卵個数を毎日計測するとともに、それらの卵に含まれる総ポリフェノール量を検査した。   In this example, a mixed feed for chickens is used as a feed material, and, as a residue of apple immature fruits, the pomace after extracting fruit juice from immature apples is 0%, 5%, 10%, 20% by weight. 50% and 50% of each mixture are prepared, each animal feed is given to 20 chickens under the same conditions, the number of eggs laid in each section is measured daily, and the total amount of polyphenols contained in those eggs is determined. Inspected.

配合飼料としては、とうもろこし、マイロなどの穀類が約60%、大豆油かす、なたね油かす、コーングルテンミールなどの植物性油かす類が約15%、魚粉、肉骨粉、フィッシュソリュブル吸着飼料などの動物性飼料が約10%、米ぬか、コーングルテンフィードなどのそうこう類が約5%、その他として炭酸カルシウムや動物性油脂、食塩、リン酸カルシウムなどが約10%配合され、その成分として、粗たんぱく質が約17%以上、粗脂肪が約2.5%以上、粗繊維が約5.0%以上、粗灰分が約13%以上、カルシウムが約2.8%以上、リンが約0.5%以上含む、一般的な鶏用の配合飼料を用いた。つまり、配合飼料には、ポリフェノールが含まれていない。
このような鶏用の配合飼料に、りんご未熟果実の残渣(未熟リンゴから果汁を抽出した後の搾りかす)が所定量ずつ添加されたものを、鶏1羽に対して1日、120gずつ与えて、21日間に亘り、各区の鶏の産卵個数を毎日計測した。
また、各区における鶏が動物用飼料を食べ残したか否を観察し、その食べ残し量についても観測した。
As for the mixed feed, corn, milo and other cereals are about 60%, soybean oil cake, rapeseed oil cake, corn gluten meal and other vegetable oil cakes are about 15%, fish meal, meat bone meal, fish soluble adsorption feed About 10% of natural feed, about 5% of rice bran, corn gluten feed and other algae, and about 10% of calcium carbonate, animal fats, salt, calcium phosphate, etc., and about 17% of crude protein % Or more, crude fat is about 2.5% or more, crude fiber is about 5.0% or more, crude ash is about 13% or more, calcium is about 2.8% or more, and phosphorus is about 0.5% or more, A general chicken formula feed was used. That is, the mixed feed does not contain polyphenol.
120 grams of chicken per day, each containing a predetermined amount of unripe apple residue (squeezed after extraction of fruit juice from unripe apples) is added to such a mixed feed for chickens. Then, the number of eggs laid in the chickens in each section was measured every day for 21 days.
In addition, the chickens in each section were observed whether or not they left the animal feed, and the amount of leftovers was also observed.

産卵結果は、りんご未熟果実の残渣となる未熟リンゴ搾りかすの含有量(重量比)別に、各区の平均産卵数を表したグラフを図1に示す。
これによれば、平均産卵数は、りんご未熟果実10%区が全体の中でも最も産卵数が多く、りんご未熟果実0%区とりんご未熟果実20%がほぼ同じであり、りんご未熟果実50%区がりんご未熟果実5%区よりも少なかった。
また、図1のグラフによれば、配合飼料に対し重量比でりんご未熟果実の残渣(未熟リンゴから果汁を抽出した後の搾りかす)を約8〜18%混合した場合の平均産卵数は、りんご未熟果実0%区の平均産卵数以上になることが解った。さらに、配合飼料に対し重量比でりんご未熟果実の残渣を51%以上混合した場合の平均産卵数は、りんご未熟果実50%区の平均産卵数よりも少なくなる傾向にあることも推測できる。
The egg-laying results are shown in FIG. 1 as a graph showing the average number of eggs laid in each section according to the content (weight ratio) of immature apple pomace that becomes the residue of immature apple fruits.
According to this, the average number of eggs laid is 10% for immature apples, the highest number of eggs in the whole, 0% for immature apples and 20% for immature apples, and 50% for immature apples. There was less than 5% of the unripe apple fruit.
Moreover, according to the graph of FIG. 1, the average number of eggs laid when about 8 to 18% of the residue of apple immature fruits (powder after extracting fruit juice from immature apples) is mixed by weight with respect to the mixed feed, It was found that the average number of eggs laid in the 0% ward of immature apples was exceeded. Furthermore, it can also be estimated that the average number of eggs laid when 51% or more of apple immature fruit residues are mixed in a weight ratio with respect to the mixed feed tends to be smaller than the average number of eggs laid in the 50% apple immature fruit.

そして、各区の鶏が産卵した卵に含まれる総ポリフェノール量(没食子酸換算:mg/100g)を、フォリン−デニス法(定量下限値:10mg/100g)により検査した(検査者:株式会社食環境衛生研究所)。
各区の卵中における総ポリフェノール量の検査結果は、図2に示すグラフのようになった。
これによれば、りんご未熟果実0%区の卵には、総ポリフェノールが検出されなかったのに対し、りんご未熟果実5%区の卵とりんご未熟果実10%区の卵に、同量の総ポリフェノールが検出され、りんご未熟果実50%区の卵が最も総ポリフェノール量が多かった。
Then, the total polyphenol content (in terms of gallic acid: mg / 100g) contained in the eggs laid by the chickens in each section was examined by the Folin-dennis method (lower limit of quantification: 10mg / 100g) (inspector: Food Environment Co., Ltd.) Institute of Health).
The test result of the total polyphenol content in the eggs of each section was as shown in the graph of FIG.
According to this, total polyphenols were not detected in the eggs of 0% apple immature fruit, whereas the same amount of total polyphenols was found in the eggs of 5% apple immature fruit and 10% apple immature fruit. Polyphenols were detected, and eggs in 50% apple immature fruits had the highest amount of total polyphenols.

鶏卵にポリフェノールが含まれる理由としては、りんごポリフェノールが多く含まれるりんご未熟果実の搾りかすが混ぜられた動物用飼料を鶏が食べることにより、各鶏の体内でりんごポリフェノールが生物濃縮されてりんごポリフェノール入りの卵が産み出されたと考えられる。   The reason why polyphenols are contained in chicken eggs is that when chickens eat animal feed mixed with squeezed fruits of immature apples that contain a large amount of apple polyphenols, the polyphenols are bio-concentrated in each chicken's body and contain apple polyphenols. It is thought that the egg was born.

さらに、りんご未熟果実20%区の卵は、りんご未熟果実5%区の卵及びりんご未熟果実10%区の卵よりも総ポリフェノール量が少なかった。
その理由としては、各区の鶏における動物用飼料の食べ残しに関係すると推測される。
動物用飼料の食べ残しは、りんご未熟果実0%区の鶏、りんご未熟果実5%区の鶏、りんご未熟果実10%区の鶏で見つからなかったが、りんご未熟果実20%区の鶏、りんご未熟果実50%区の鶏でそれぞれ観察された。それらの食べ残した量は、りんご未熟果実20%区の鶏よりもりんご未熟果実50%区の鶏が多かった。
つまり、各区の鶏1羽に対して1日に与える動物用飼料の総量(120g)に同じであるにかかわらず、りんご未熟果実の残渣となる未熟リンゴ搾りかすの含有量が一定量よりも多くなると、食べ残しが見られ、未熟リンゴ搾りかすの含有量が多くなるほど、食べ残した量も増えることが解った。鶏は、配合飼料に対する未熟リンゴ搾りかすの含有比が所定以上になると、食欲不振になることが判明した。
Furthermore, the eggs of the 20% apple immature fruit had less total polyphenols than the eggs of the apple immature fruit 5% and the apple immature fruit 10%.
The reason is presumed to be related to uneaten animal feed in chickens in each section.
No food leftovers were found in 0% immature apples, 5% immature apples, and 10% immature apples, but 20% immature apples and apples It was observed in chickens of 50% immature fruit. The amount of those left over was higher in 50% immature apples than in 20% immature apples.
In other words, the content of immature apple pomace that becomes the residue of unripe apple fruit is greater than a certain amount, despite being the same as the total amount of animal feed (120 g) given to one chicken in each section per day. As a result, it was found that leftovers were observed, and that the amount of leftovers increased as the content of unripe apple pomace increased. It has been found that chickens lose their appetite when the content ratio of immature apple pomace with respect to the blended feed exceeds a predetermined level.

それにより、りんご未熟果実20%区の鶏は、動物用飼料の食べ残しがあったため、同区における鶏の体内で生物濃縮されるりんごポリフェノールの量が減少して、産卵した卵に含まれる総ポリフェノール量も減少したと推測される。
りんご未熟果実50%区の鶏は、りんご未熟果実20%区の鶏よりも食べ残し量が多かったものの、配合飼料に対する未熟リンゴ搾りかすの含有量がりんご未熟果実20%区よりも多いため、同区における鶏の体内で生物濃縮されるりんごポリフェノールの量が多くなり、産卵した卵に含まれる総ポリフェノール量が、りんご未熟果実5%区の卵及びりんご未熟果実10%区の卵よりも多くなったと推測される。
As a result, chickens in the 20% apple immature fruit group had left over food for animal feed, so the amount of apple polyphenol bioconcentrated in the chicken body in the same area decreased, and the total eggs contained in the eggs laid. It is estimated that the amount of polyphenol has also decreased.
The chicken with 50% immature apples had more leftover than the chicken with 20% immature apples, but the content of immature apple pomace in the mixed feed was higher than the 20% immature apples. The amount of apple polyphenol that is bio-concentrated in the chicken body in the same ward increases, and the total amount of polyphenol contained in the eggs laid is higher than that of the 5% apple immature fruit and 10% apple immature fruit eggs. Presumed to be.

また、図2のグラフによれば、配合飼料に対し重量比でりんご未熟果実の残渣(未熟リンゴから果汁を抽出した後の搾りかす)が5%未満混合された動物用飼料を鶏に与えた場合は、未熟リンゴ搾りかすの含有量が減ることに従って、産卵した卵に含まれる総ポリフェノール量が少なくなる傾向がある。
その理由としては、配合飼料に対する未熟リンゴ搾りかすの含有量が少なくなるため、鶏の体内で生物濃縮されるりんごポリフェノールの量が減少して、産卵した卵に含まれる総ポリフェノール量も減少すると推測される。
Moreover, according to the graph of FIG. 2, the animal feed in which the residue of the apple immature fruit (powder after extracting fruit juice from an immature apple) was mixed with the mixed feed by less than 5% was given to the chicken. In this case, as the content of the immature apple pomace decreases, the total amount of polyphenol contained in the egg laid tends to decrease.
The reason for this is that the content of immature apple pomace in the blended feed is reduced, so the amount of apple polyphenol bioconcentrated in the body of the chicken is reduced, and the total polyphenol content in the eggs laid is also reduced. Is done.

またさらに、図2のグラフによれば、配合飼料に対し重量比でりんご未熟果実の残渣が51%以上混合された動物用飼料を鶏に与えた場合は、産卵した卵に含まれる総ポリフェノール量が更に多くなる傾向がある。
しかし、配合飼料に対する未熟リンゴ搾りかすの含有量が多くなると、未熟リンゴ搾りかすの含有量が増えることに従って配合飼料に元々含まれる栄養分は少なくなり、りんご未熟果実の残渣(未熟リンゴから果汁を抽出した後の搾りかす)には配合飼料の栄養分が含まれていないため、長期間に亘って、配合飼料に対し重量比でりんご未熟果実の残渣が51%以上混合した動物用飼料を与え続けると、配合飼料に含まれる栄養分が不足して動物が成育不良になり、悪影響を与えることが産卵数の減少から判明した。
さらに、配合飼料に対する未熟リンゴ搾りかすの含有量が増えることに従って、動物用飼料の食べ残しが増加して無駄になることも推測される。
Furthermore, according to the graph of FIG. 2, when an animal feed in which 51% or more of apple immature fruit residue is mixed by weight with respect to the blended feed is given to chickens, the total amount of polyphenols contained in the eggs laid Tend to be more.
However, when the content of immature apple pomace in the mixed feed increases, the nutrient content originally contained in the mixed feed decreases as the content of the immature apple pomace increases, and the apple immature fruit residue (extract the juice from the immature apple) The squeezed pomace) does not contain the nutrients of the blended feed, so if you continue to feed the blended animal feed with a mixture of 51% or more of immature apple residues by weight with respect to the blended feed over a long period of time It was found from the decrease in the number of eggs laid that the nutrients contained in the mixed feed were insufficient and the animals became poorly grown and had adverse effects.
Furthermore, it is estimated that the amount of uneaten animal feed increases and is wasted as the content of immature apple pomace increases with respect to the blended feed.

したがって、配合飼料に対し重量比でりんご未熟果実の残渣が5〜50%混合された動物用飼料を与えた鶏の卵に、りんごポリフェノールが多く含まれることを実験によって確認できた。
さらに、配合飼料に対し重量比でりんご未熟果実の残渣が約8〜18%混合された動物用飼料を与えた鶏は、りんご未熟果実の残渣が混合されない動物用飼料を与えた鶏に比べて、産卵数が多くなると同時に、その卵にりんごポリフェノールが多く含まれることも実験によって確認できた。
特に、配合飼料に対し重量比でりんご未熟果実の残渣が10%混合された動物用飼料を与えた鶏は、最も多く産卵し、且つその卵にりんごポリフェノールが多く含まれることも実験によって確認できた。
それにより、配合飼料に対し重量比でりんご未熟果実の残渣が10%混合された動物用飼料を与えた鶏は、りんご未熟果実の残渣の添加量(未熟リンゴ搾りかすの含有量)が比較的少ない動物用飼料で育成されたことにかかわらず、多量のりんごポリフェノールが含まれる卵を多く産卵することになる。
Accordingly, it was confirmed by experiments that chicken polyphenols were contained in a large amount of chicken eggs fed with animal feed in which 5 to 50% of apple immature fruit residues were mixed by weight with respect to the blended feed.
Furthermore, chickens fed with animal feed in which about 8-18% of apple immature fruit residues were mixed by weight with respect to the blended feed were compared to chickens fed with animal feed that was not mixed with apple immature fruit residues. It was also confirmed by experiments that the number of eggs laid increased and that the eggs contained a lot of apple polyphenols.
In particular, it was confirmed by experiments that chickens fed with animal feed in which 10% of apple immature fruit residue was mixed by weight with respect to the mixed feed lay eggs and that the eggs contained a large amount of apple polyphenols. It was.
As a result, chickens fed with animal feed in which 10% of apple immature fruit residue was mixed by weight with respect to the compounded feed had a relatively small amount of apple immature fruit residue (content of immature apple pomace). Despite being cultivated with a small amount of animal feed, many eggs containing a large amount of apple polyphenol will be laid.

一方、別の実験で、各区における鶏の肉や鶏から排出される糞を検査した結果、りんご未熟果実0%区にはポリフェノールが検出されなかったのに対し、それ以外の区の鶏肉や鶏糞にはポリフェノールが検出された。   On the other hand, in another experiment, as a result of examination of chicken meat and feces excreted from chickens in each ward, polyphenol was not detected in the 0% apple immature fruit ward, whereas chicken and chicken shit in the other wards Polyphenol was detected in.

これら鶏肉や鶏糞にポリフェノールが含まれる理由としては、りんごポリフェノールが多く含まれるりんご未熟果実の残渣(未熟リンゴから果汁を抽出した後の搾りかす)が混ぜられた動物用飼料を鶏が食べることにより、各鶏の体内でりんごポリフェノールが生物濃縮されて、鶏の肉にりんごポリフェノールが含まれるとともに、りんごポリフェノール入りの糞が排出されると考えられる。
特に、配合飼料に対し重量比でりんご未熟果実の残渣が約8〜18%、好ましくは10%混合された動物用飼料を鶏に与えた場合には、前述した実験に基づいて、りんご未熟果実の残渣の添加量(未熟リンゴ搾りかすの含有量)が比較的少ない動物用飼料で育成されたことにかかわらず、鶏の体内で多量のりんごポリフェノールが生物濃縮されて、鶏の肉に多量のりんごポリフェノールが含まれるとともに、多量のりんごポリフェノールが入った糞が排出されると考えられる。
The reason why polyphenols are contained in these chicken meat and chicken dung is that chickens eat animal feed mixed with apple immature fruit residue (squeezed after extracting fruit juice from immature apples) rich in apple polyphenols. It is considered that apple polyphenols are bio-concentrated in the body of each chicken, so that chicken polyphenols are contained in chicken meat and feces containing apple polyphenols are discharged.
In particular, when an animal feed containing about 8 to 18%, preferably 10%, of a mixture of immature apple fruits by weight with respect to the blended feed is given to chickens, based on the above-described experiment, Despite being grown on animal feed with a relatively small amount of residue added (content of immature apple pomace), a large amount of apple polyphenol is bioconcentrated in the chicken body, It is considered that feces containing a large amount of apple polyphenol are discharged while containing apple polyphenol.

なお、前示実施例では、本発明の家畜用飼料を鶏に与えた場合のみを示したが、これに限定されず、鶏以外に例えば牛・豚・馬などの家畜やペットや養魚に与えても良い。
これらの場合においても、前示実施例と同様に、本発明の家畜用飼料が与えられた牛・豚・馬や養魚などの体内でポリフェノールが生物濃縮されて、りんごポリフェノールを含んだ肉や乳汁などが得られることは容易に理解できる。
In the previous examples, only the case where the feed for livestock of the present invention was given to chickens was shown, but this is not a limitation, and it is given to livestock such as cattle, pigs and horses, pets and fish farms in addition to chickens. May be.
Also in these cases, as in the previous examples, meat or milk containing apple polyphenols is obtained by bioconcentrating polyphenols in the body of cattle, pigs, horses, fish, etc. to which the livestock feed of the present invention is applied. Etc. can be easily understood.

Claims (3)

飼料材料に対し、その重量比でりんご未熟果実の残渣として、未熟リンゴから果汁を抽出した後の搾りかすとなる外皮部位及びその周辺部位をそのまま何も加工せずに5〜50%混合したことを特徴とする動物用飼料。   Mixing 5 to 50% of the feed material with the unprocessed apple skin as a residue of unripe apples in the weight ratio without any processing of the outer skin part and its peripheral part that would be squeezed after extracting fruit juice from the immature apple Animal feed characterized by 前記飼料材料が配合飼料であることを特徴とする請求項1に記載の動物用飼料。   The animal feed according to claim 1, wherein the feed material is a mixed feed. 前記飼料材料に対し、その重量比で前記りんご未熟果実の残渣を8〜18%混合したことを特徴とする請求項1又は2に記載の動物用飼料 The animal feed according to claim 1 or 2, wherein 8 to 18% of the apple immature fruit residue is mixed in the weight ratio with the feed material .
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