JP5540102B2 - 高められた病理学的決定のための複数モダリティコントラストおよび明視野コンテキスト表現、および組織内の複数検体検出 - Google Patents

高められた病理学的決定のための複数モダリティコントラストおよび明視野コンテキスト表現、および組織内の複数検体検出 Download PDF

Info

Publication number
JP5540102B2
JP5540102B2 JP2012533322A JP2012533322A JP5540102B2 JP 5540102 B2 JP5540102 B2 JP 5540102B2 JP 2012533322 A JP2012533322 A JP 2012533322A JP 2012533322 A JP2012533322 A JP 2012533322A JP 5540102 B2 JP5540102 B2 JP 5540102B2
Authority
JP
Japan
Prior art keywords
image
contrast
images
color
specimen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2012533322A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013507612A (ja
Inventor
ガーシャ,カール
ペスタノ,ゲイリー
オッター,マイケル
ナジー,アレクサンドラ・ディア
ネイグル,レイ・ビー
ミラー,フィリップ
フロリック,ジャン
デイ,ウィリアム
Original Assignee
ベンタナ メディカル システムズ, インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ベンタナ メディカル システムズ, インコーポレイテッド filed Critical ベンタナ メディカル システムズ, インコーポレイテッド
Publication of JP2013507612A publication Critical patent/JP2013507612A/ja
Application granted granted Critical
Publication of JP5540102B2 publication Critical patent/JP5540102B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging

Landscapes

  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP2012533322A 2009-10-12 2010-10-07 高められた病理学的決定のための複数モダリティコントラストおよび明視野コンテキスト表現、および組織内の複数検体検出 Expired - Fee Related JP5540102B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US25080909P 2009-10-12 2009-10-12
US61/250,809 2009-10-12
US27893609P 2009-10-13 2009-10-13
US61/278,936 2009-10-13
PCT/US2010/051857 WO2011046807A2 (en) 2009-10-12 2010-10-07 Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue

Publications (2)

Publication Number Publication Date
JP2013507612A JP2013507612A (ja) 2013-03-04
JP5540102B2 true JP5540102B2 (ja) 2014-07-02

Family

ID=43876802

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012533322A Expired - Fee Related JP5540102B2 (ja) 2009-10-12 2010-10-07 高められた病理学的決定のための複数モダリティコントラストおよび明視野コンテキスト表現、および組織内の複数検体検出

Country Status (10)

Country Link
US (1) US9310302B2 (enExample)
EP (1) EP2488860A4 (enExample)
JP (1) JP5540102B2 (enExample)
KR (1) KR101388291B1 (enExample)
CN (1) CN102667473B (enExample)
AU (1) AU2010307140B2 (enExample)
CA (1) CA2774422C (enExample)
IL (1) IL218553B (enExample)
IN (1) IN2012DN02337A (enExample)
WO (1) WO2011046807A2 (enExample)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9767550B2 (en) * 2010-12-13 2017-09-19 Koninklijke Philips N.V. Method and device for analysing a region of interest in an object using x-rays
US8639013B2 (en) * 2011-08-17 2014-01-28 General Electric Company System and methods for generating a brightfield image using fluorescent images
JP5826561B2 (ja) * 2011-08-24 2015-12-02 オリンパス株式会社 顕微鏡システム、標本画像生成方法及びプログラム
JP5784435B2 (ja) * 2011-09-20 2015-09-24 オリンパス株式会社 画像処理装置、蛍光顕微鏡装置および画像処理プログラム
CN102426124B (zh) * 2011-11-08 2013-07-03 中国人民解放军第四军医大学 用于病理组织冰冻切片的捞片展片装置及方法
SG193046A1 (en) * 2012-02-23 2013-09-30 Histoindex Pte Ltd A digital imaging system for biopsy inspection
US9194800B2 (en) * 2012-10-29 2015-11-24 Tokitae Llc Systems, devices, and methods employing angular-resolved scattering and spectrally resolved measurements for classification of objects
KR20150094618A (ko) * 2012-12-06 2015-08-19 클래리언트 다이아그노스틱 서비시즈, 인크. 생물학적 시편의 분할 스크린 디스플레이와 그 레코드를 포착하기 위한 시스템 및 방법
RU2012156158A (ru) * 2012-12-24 2014-07-10 ЭлЭсАй Корпорейшн Генерация целевого изображения с использованием функционала на основе функций от информации из других изображений
JP6503335B2 (ja) * 2013-03-12 2019-04-17 サイトヴィヴァ, インコーポレイテッド 生物学的な及び非生物学的な媒質でナノ粒子の位置を特定するための3次元画像処理
US9786050B2 (en) * 2013-03-15 2017-10-10 The Board Of Trustees Of The University Of Illinois Stain-free histopathology by chemical imaging
CN113349707A (zh) 2013-12-31 2021-09-07 纪念斯隆-凯特琳癌症中心 用于荧光源实时多通道成像的系统、方法和设备
TW201546486A (zh) * 2014-06-11 2015-12-16 Univ Nat Cheng Kung 利用數位微型反射鏡元件之多光子螢光激發顯微裝置
GB2528283B (en) 2014-07-16 2020-08-05 Barco Nv Image colour calibration with multiple colour scales
JP6531403B2 (ja) * 2015-01-27 2019-06-19 株式会社ニコン 顕微鏡
EP3268739B8 (en) * 2015-03-11 2023-10-11 The General Hospital Corporation Plasmonic nanoparticle immunoassay method
US10672505B2 (en) 2015-06-03 2020-06-02 General Electric Company Biological data annotation and visualization
US9953133B2 (en) 2015-06-03 2018-04-24 General Electric Company Biological data annotation and visualization
WO2016201186A1 (en) * 2015-06-11 2016-12-15 University Of Pittsburgh-Of The Commonwealth System Of Higher Education Systems and methods for finding regions of interest in hematoxylin and eosin (h&e) stained tissue images and quantifying intratumor cellular spatial heterogeneity in multiplexed/hyperplexed fluorescence tissue images
JP6451845B2 (ja) * 2015-06-16 2019-01-16 コニカミノルタ株式会社 病理標本の蛍光画像の取得方法
CN106338423B (zh) 2015-07-10 2020-07-14 三斯坎公司 组织学染色的空间复用
WO2017060954A1 (ja) 2015-10-05 2017-04-13 オリンパス株式会社 撮像装置
JP6703824B2 (ja) 2015-11-30 2020-06-03 シスメックス株式会社 細胞選択方法、細胞検出方法、細胞選択装置、および細胞検出装置
JP6597316B2 (ja) * 2016-01-06 2019-10-30 コニカミノルタ株式会社 画像処理装置及びプログラム
US10761027B2 (en) * 2016-01-19 2020-09-01 Konica Minolta, Inc. Image processing apparatus and computer-readable recording medium storing program
CN108885210B (zh) 2016-02-05 2022-07-19 纳诺韦尔生物科学有限公司 具有表面标记物的外来体的检测
MA43593A (fr) 2016-02-08 2018-11-14 Imago Systems Inc Système et procédé de visualisation et de caractérisation d'objets dans des images
US10825195B2 (en) * 2016-04-06 2020-11-03 Hoffman-La Roche, Inc. Spatial index creation for IHC image analysis
JP6755975B2 (ja) * 2016-06-10 2020-09-16 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト 明視野像シミュレーションのためのシステム
EP3264741A1 (en) * 2016-06-30 2018-01-03 Thomson Licensing Plenoptic sub aperture view shuffling with improved resolution
US10324041B2 (en) 2016-12-21 2019-06-18 Abbott Japan Co., Ltd. Optical imaging system using lateral illumination for digital assays
US11047854B2 (en) 2017-02-06 2021-06-29 Abbott Japan Llc Methods for reducing noise in signal-generating digital assays
US10580130B2 (en) * 2017-03-24 2020-03-03 Curadel, LLC Tissue identification by an imaging system using color information
TWI654443B (zh) * 2017-06-05 2019-03-21 銳準醫光股份有限公司 採用進階光學干涉顯微術之光學切層裝置
JP7014794B2 (ja) 2017-07-11 2022-02-01 浜松ホトニクス株式会社 試料観察装置及び試料観察方法
WO2019012776A1 (ja) * 2017-07-11 2019-01-17 浜松ホトニクス株式会社 試料観察装置及び試料観察方法
TWI659395B (zh) * 2017-07-19 2019-05-11 AcuSolutions Inc. 類h&e影像之合成方法及採用該方法之光學系統
WO2019074926A1 (en) * 2017-10-09 2019-04-18 Pathspot Technologies Inc. SYSTEMS AND METHODS FOR DETECTION OF CONTAMINANTS ON SURFACES
US10446113B2 (en) * 2018-01-30 2019-10-15 ForeFlight LLC Method and system for inversion of raster images
US11783603B2 (en) 2018-03-07 2023-10-10 Verily Life Sciences Llc Virtual staining for tissue slide images
TWI683283B (zh) * 2018-04-03 2020-01-21 薩摩亞商銳準醫光股份有限公司 一種生物樣本之影像合成方法及採用該方法之光學系統
JP7298993B2 (ja) 2018-04-09 2023-06-27 浜松ホトニクス株式会社 試料観察装置及び試料観察方法
JP7007227B2 (ja) * 2018-04-09 2022-01-24 浜松ホトニクス株式会社 試料観察装置及び試料観察方法
US10585042B2 (en) * 2018-06-06 2020-03-10 Trustees Of Boston University Systems and methods for imaging microwell plate samples
CN110648371B (zh) * 2018-06-27 2023-05-09 阿里巴巴集团控股有限公司 Rgb值与色号信息映射关系的确定方法及装置
JP2021529951A (ja) * 2018-06-28 2021-11-04 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California 画像データを組み合わせることによる染色組織試料の合成画像の作成
WO2020039520A1 (ja) * 2018-08-22 2020-02-27 オリンパス株式会社 画像処理装置、撮像システム、画像処理装置の作動方法、及び画像処理装置の作動プログラム
US12016690B2 (en) * 2019-02-12 2024-06-25 Purdue Research Foundation Characterization of injection-induced tissue swelling during subcutaneous injection of biologics
CN113424050B (zh) 2019-02-13 2025-01-10 文塔纳医疗系统公司 用于计算多通道图像中的自体荧光贡献的系统和方法
JP7470339B2 (ja) * 2019-03-06 2024-04-18 学校法人 埼玉医科大学 染色画像推定器学習装置、画像処理装置、染色画像推定器学習方法、画像処理方法、染色画像推定器学習プログラム、及び、画像処理プログラム
EP3709262A1 (en) * 2019-03-13 2020-09-16 Koninklijke Philips N.V. Correlated image analysis for 3d biopsy
JP7652762B2 (ja) * 2019-08-28 2025-03-27 ベンタナ メディカル システムズ, インコーポレイテッド 振動分光法によるバイオマーカー発現の無標識評価
EP4038370A4 (en) * 2019-10-03 2023-10-11 The Regents of the University of California Fluorescence imitating brightfield imaging
EP3805838B1 (en) * 2019-10-10 2023-12-06 Leica Instruments (Singapore) Pte. Ltd. Microscope and related apparatuses, methods and computer programs
WO2021081253A1 (en) 2019-10-22 2021-04-29 Tempus Labs, Inc. Systems and methods for predicting therapeutic sensitivity
KR102302333B1 (ko) * 2019-11-05 2021-09-16 주식회사 토모큐브 3차원 굴절률 영상과 딥러닝을 활용한 비표지 방식의 3차원 분자 영상 생성 방법 및 장치
US11415571B2 (en) 2019-12-05 2022-08-16 Tempus Labs, Inc. Large scale organoid analysis
WO2021155378A1 (en) * 2020-02-01 2021-08-05 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for performing multiple-wavelength quantitative phase imaging (qpi)
FR3109159B1 (fr) * 2020-04-09 2023-06-23 Interscience Procédé de traitement d’images appliqué aux compteurs de colonies en microbiologie
US11561178B2 (en) * 2020-04-20 2023-01-24 Tempus Labs, Inc. Artificial fluorescent image systems and methods
EP3907497B1 (en) * 2020-05-08 2023-08-02 Leica Microsystems CMS GmbH Apparatus and method for displaying and/or printing images of a specimen including a fluorophore
WO2022030201A1 (ja) * 2020-08-03 2022-02-10 コニカミノルタ株式会社 測光装置
JP7492881B2 (ja) * 2020-08-03 2024-05-30 株式会社日本マイクロニクス 測定システムおよび測定方法
US12251086B2 (en) 2020-09-03 2025-03-18 University Of Washington Optical fixation monitor
US12014830B2 (en) * 2021-04-18 2024-06-18 Mary Hitchcock Memorial Hospital, For Itself And On Behalf Of Dartmouth-Hitchcock Clinic System and method for automation of surgical pathology processes using artificial intelligence
CN115656120B (zh) * 2022-10-09 2024-07-23 中国科学院深圳先进技术研究院 一种暗场反射紫外光学显微成像方法和系统
DE102022130251A1 (de) 2022-11-16 2024-05-16 Carl Zeiss Microscopy Gmbh Mikroskopanordnung und Verfahren zur Untersuchung einer mit mehreren Farbstoffen gefärbten Probe
WO2025076554A1 (en) * 2023-10-06 2025-04-10 The Regents Of The University Of California Targeted cell profiling via in situ hybridization with mass spectrometry detection
CN117706102B (zh) * 2024-02-05 2024-04-12 遂宁市中心医院 一种肿瘤康复用光学检测装置及检测方法

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4288323A (en) 1979-02-05 1981-09-08 Brigante Miguel F Free flow non-corrosive water treatment device
US5162990A (en) 1990-06-15 1992-11-10 The United States Of America As Represented By The United States Navy System and method for quantifying macrophage phagocytosis by computer image analysis
US5249077A (en) 1991-12-12 1993-09-28 Microvideo Instruments, Inc. Darkfield illuminator for a microscope slide
US6007994A (en) * 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
JP4348574B2 (ja) 1998-04-30 2009-10-21 株式会社ニコン 暗視野照明装置および暗視野照明方法
CA2243090A1 (en) 1998-07-10 2000-01-10 Timothy M. Richardson Inverted darkfield contrast microscope and method
US8406498B2 (en) * 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US7450229B2 (en) * 1999-01-25 2008-11-11 Amnis Corporation Methods for analyzing inter-cellular phenomena
US8131053B2 (en) * 1999-01-25 2012-03-06 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US6403947B1 (en) 1999-03-18 2002-06-11 Cambridge Research & Instrumentation Inc. High-efficiency multiple probe imaging system
US6649351B2 (en) 1999-04-30 2003-11-18 Aclara Biosciences, Inc. Methods for detecting a plurality of analytes by mass spectrometry
AU782408B2 (en) 1999-05-07 2005-07-28 Yale University Multiple tag analysis
US6195451B1 (en) 1999-05-13 2001-02-27 Advanced Pathology Ststems, Inc. Transformation of digital images
WO2001008552A1 (en) 1999-08-03 2001-02-08 Biophysica, Llc Spectroscopic systems and methods for detecting tissue properties
US6649138B2 (en) 2000-10-13 2003-11-18 Quantum Dot Corporation Surface-modified semiconductive and metallic nanoparticles having enhanced dispersibility in aqueous media
US20020083888A1 (en) 2000-12-28 2002-07-04 Zehnder Donald A. Flow synthesis of quantum dot nanocrystals
AU2002367778A1 (en) 2001-07-20 2003-11-10 Quantum Dot Corporation Luminescent nanoparticles and methods for their preparation
DE10144250A1 (de) 2001-08-31 2003-04-03 Fraunhofer Ges Forschung Verbesserte massenspektrometrische Analyse unter Verwendung von Nanopartikeln
JP2006014868A (ja) * 2004-06-30 2006-01-19 Hamamatsu Photonics Kk リンパ節検出装置
US8260013B2 (en) * 2004-11-22 2012-09-04 Koninklijke Philips Electronics N.V. Data representation for rtp
EP1681015A1 (en) 2005-01-17 2006-07-19 Imasys SA Temperature mapping on structural data
US7524631B2 (en) * 2005-02-02 2009-04-28 Patterson Bruce K HPV E6, E7 mRNA assay and methods of use thereof
US8041409B2 (en) * 2005-09-08 2011-10-18 Carestream Health, Inc. Method and apparatus for multi-modal imaging
KR100708932B1 (ko) 2005-10-06 2007-04-17 삼성전기주식회사 영상 처리장치 및 그 방법
EP1780672A1 (en) 2005-10-25 2007-05-02 Bracco Imaging, S.P.A. Method of registering images, algorithm for carrying out the method of registering images, a program for registering images using the said algorithm and a method of treating biomedical images to reduce imaging artefacts caused by object movement
US20090088332A1 (en) 2005-11-21 2009-04-02 Jingyue Ju Multiplex Digital Immuno-Sensing Using a Library of Photocleavable Mass Tags
US8010184B2 (en) 2005-11-30 2011-08-30 General Electric Company Method and apparatus for automatically characterizing a malignancy
WO2007064959A2 (en) 2005-12-01 2007-06-07 Auburn University High resolution optical microscope
CN101351736A (zh) * 2005-12-27 2009-01-21 奥林巴斯株式会社 发光测量装置及发光测量方法
WO2007074923A1 (ja) 2005-12-27 2007-07-05 Olympus Corporation 発光測定装置並びに発光測定方法
JP4614000B2 (ja) * 2006-04-07 2011-01-19 株式会社島津製作所 質量分析装置
WO2008039758A2 (en) 2006-09-25 2008-04-03 Cambridge Research & Instrumentation, Inc. Sample imaging and classification
US8244021B2 (en) 2006-12-20 2012-08-14 Ventana Medical Systems, Inc. Quantitative, multispectral image analysis of tissue specimens stained with quantum dots
US7327859B1 (en) * 2007-02-14 2008-02-05 Lam Ko Chau Methods and systems for automated fingerprint recognition
JP5013410B2 (ja) * 2007-03-14 2012-08-29 独立行政法人産業技術総合研究所 微粒子測定方法及び装置
US7819489B2 (en) 2007-03-20 2010-10-26 Kellogg Company Concurrently printing an image on a food product and a corresponding image on packaging for the food product
US20090129650A1 (en) * 2007-11-19 2009-05-21 Carestream Health, Inc. System for presenting projection image information
CN101216601B (zh) 2007-12-29 2010-12-15 中国科学院西安光学精密机械研究所 使用锥镜实现暗场显微及荧光显微的方法及装置
JP4288323B1 (ja) * 2008-09-13 2009-07-01 独立行政法人科学技術振興機構 顕微鏡装置及びそれを用いた蛍光観察方法
US8849006B2 (en) * 2009-03-25 2014-09-30 Quorum Technologies Inc. Darkfield imaging system and methods for automated screening of cells
US8948488B2 (en) * 2009-07-31 2015-02-03 General Electric Company Methods and systems for digitally enhancing an image of a stained material
JP5338573B2 (ja) * 2009-08-31 2013-11-13 ソニー株式会社 蛍光像取得方法、蛍光像取得装置及び蛍光像取得プログラム
US8269827B2 (en) * 2009-09-29 2012-09-18 General Electric Company System and methods for mapping fluorescent images into a bright field color space
EP2601513B1 (en) * 2010-08-05 2014-05-14 Cambridge Research & Instrumentation, Inc. Enhancing visual assessment of samples

Also Published As

Publication number Publication date
JP2013507612A (ja) 2013-03-04
CA2774422C (en) 2017-08-29
AU2010307140B2 (en) 2014-05-15
EP2488860A4 (en) 2015-12-16
IL218553B (en) 2018-01-31
WO2011046807A2 (en) 2011-04-21
CN102667473A (zh) 2012-09-12
EP2488860A2 (en) 2012-08-22
IL218553A0 (en) 2012-05-31
KR20120066654A (ko) 2012-06-22
US9310302B2 (en) 2016-04-12
CN102667473B (zh) 2016-06-08
IN2012DN02337A (enExample) 2015-08-21
CA2774422A1 (en) 2011-04-21
AU2010307140A1 (en) 2012-04-05
WO2011046807A3 (en) 2011-09-01
KR101388291B1 (ko) 2014-04-22
US20120200694A1 (en) 2012-08-09

Similar Documents

Publication Publication Date Title
JP5540102B2 (ja) 高められた病理学的決定のための複数モダリティコントラストおよび明視野コンテキスト表現、および組織内の複数検体検出
EP2601513B1 (en) Enhancing visual assessment of samples
AU2015318251B2 (en) System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents
US9964489B2 (en) System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents
US10846848B2 (en) System for bright field image simulation
US10032064B2 (en) Visualization and measurement of cell compartments
JP7418631B2 (ja) マルチチャネル画像における自己蛍光の寄与を算出するシステムおよび方法
JP7565621B2 (ja) 蛍光模倣明視野イメージング
WO2018175565A1 (en) System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents
US7316904B1 (en) Automated pap screening using optical detection of HPV with or without multispectral imaging
Levenson et al. Modern Trends in Imaging X: Spectral Imaging in Preclinical Research and Clinical Pathology
WO2023149296A1 (ja) 情報処理装置、生体試料観察システム及び画像生成方法
Levenson et al. Spectral imaging in preclinical research and clinical pathology
HK1171508A (en) Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue
HK1171508B (en) Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue
BR112012008484A2 (pt) método, aparelho de formação de imagens, pelo menos um meio de armazenagem legível por computador e processador de imagens
HK40003631A (zh) 用於明场图像模拟的系统
HK40003631B (zh) 用於生成模拟数字明场ihc或ish图像的方法和图像处理系统
HK1239808A1 (en) System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents

Legal Events

Date Code Title Description
A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20130821

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130906

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20131126

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20140402

R150 Certificate of patent or registration of utility model

Ref document number: 5540102

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20140501

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees