JP5394893B2 - レーザ走査型顕微鏡 - Google Patents
レーザ走査型顕微鏡 Download PDFInfo
- Publication number
- JP5394893B2 JP5394893B2 JP2009256148A JP2009256148A JP5394893B2 JP 5394893 B2 JP5394893 B2 JP 5394893B2 JP 2009256148 A JP2009256148 A JP 2009256148A JP 2009256148 A JP2009256148 A JP 2009256148A JP 5394893 B2 JP5394893 B2 JP 5394893B2
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- Prior art keywords
- gain
- wavelength
- photodetector
- scanning
- cell
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 230000003595 spectral effect Effects 0.000 claims description 35
- 239000000975 dye Substances 0.000 claims description 21
- 230000035945 sensitivity Effects 0.000 claims description 21
- 239000007850 fluorescent dye Substances 0.000 claims description 12
- 238000012545 processing Methods 0.000 claims description 10
- 238000001514 detection method Methods 0.000 claims description 9
- 230000003321 amplification Effects 0.000 claims description 5
- 230000001678 irradiating effect Effects 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 230000001360 synchronised effect Effects 0.000 claims description 2
- 230000003287 optical effect Effects 0.000 description 26
- 238000012986 modification Methods 0.000 description 14
- 230000004048 modification Effects 0.000 description 14
- 238000000034 method Methods 0.000 description 12
- 238000001228 spectrum Methods 0.000 description 7
- PNDZEEPOYCVIIY-UHFFFAOYSA-N indo-1 Chemical compound CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC=2C(=CC=C(C=2)C=2N=C3[CH]C(=CC=C3C=2)C(O)=O)N(CC(O)=O)CC(O)=O)=C1 PNDZEEPOYCVIIY-UHFFFAOYSA-N 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 229910001424 calcium ion Inorganic materials 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000000049 pigment Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000005284 excitation Effects 0.000 description 4
- 230000004888 barrier function Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 238000004611 spectroscopical analysis Methods 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 239000003269 fluorescent indicator Substances 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009256148A JP5394893B2 (ja) | 2009-11-09 | 2009-11-09 | レーザ走査型顕微鏡 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009256148A JP5394893B2 (ja) | 2009-11-09 | 2009-11-09 | レーザ走査型顕微鏡 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011100058A JP2011100058A (ja) | 2011-05-19 |
| JP2011100058A5 JP2011100058A5 (enExample) | 2012-12-20 |
| JP5394893B2 true JP5394893B2 (ja) | 2014-01-22 |
Family
ID=44191266
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009256148A Expired - Fee Related JP5394893B2 (ja) | 2009-11-09 | 2009-11-09 | レーザ走査型顕微鏡 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP5394893B2 (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6257156B2 (ja) * | 2013-03-04 | 2018-01-10 | オリンパス株式会社 | 顕微鏡装置 |
| WO2020044860A1 (ja) * | 2018-08-28 | 2020-03-05 | ソニー株式会社 | 分光イメージング装置および蛍光観察装置 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11183806A (ja) * | 1997-12-18 | 1999-07-09 | Nikon Corp | コンフォーカル顕微鏡 |
| DE10038526B4 (de) * | 2000-08-08 | 2004-09-02 | Carl Zeiss Jena Gmbh | Verfahren und Anordnung zur Erfassung des wellenlängenabhängigen Verhaltens einer beleuchteten Probe |
| JP2009244156A (ja) * | 2008-03-31 | 2009-10-22 | Nikon Corp | 分光装置、及び分光共焦点顕微鏡 |
-
2009
- 2009-11-09 JP JP2009256148A patent/JP5394893B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2011100058A (ja) | 2011-05-19 |
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