JP5345078B2 - 脂質二重膜、それを形成するために用いられる自己支持性フィルム及びそれを具備するマイクロ流路デバイス - Google Patents
脂質二重膜、それを形成するために用いられる自己支持性フィルム及びそれを具備するマイクロ流路デバイス Download PDFInfo
- Publication number
- JP5345078B2 JP5345078B2 JP2010012300A JP2010012300A JP5345078B2 JP 5345078 B2 JP5345078 B2 JP 5345078B2 JP 2010012300 A JP2010012300 A JP 2010012300A JP 2010012300 A JP2010012300 A JP 2010012300A JP 5345078 B2 JP5345078 B2 JP 5345078B2
- Authority
- JP
- Japan
- Prior art keywords
- self
- lipid bilayer
- hole
- microchannel
- supporting film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000232 Lipid Bilayer Substances 0.000 title description 69
- 239000012528 membrane Substances 0.000 title description 68
- 239000011148 porous material Substances 0.000 claims abstract description 44
- 238000000034 method Methods 0.000 claims abstract description 12
- 230000009467 reduction Effects 0.000 claims abstract description 5
- 150000001875 compounds Chemical class 0.000 claims description 15
- 238000007740 vapor deposition Methods 0.000 claims description 15
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 239000000178 monomer Substances 0.000 claims description 6
- 150000002632 lipids Chemical class 0.000 abstract description 15
- 239000000463 material Substances 0.000 abstract description 9
- 108090000623 proteins and genes Proteins 0.000 description 17
- 102000004169 proteins and genes Human genes 0.000 description 17
- 229920000642 polymer Polymers 0.000 description 13
- 239000000243 solution Substances 0.000 description 13
- URLKBWYHVLBVBO-UHFFFAOYSA-N Para-Xylene Chemical group CC1=CC=C(C)C=C1 URLKBWYHVLBVBO-UHFFFAOYSA-N 0.000 description 10
- 238000000151 deposition Methods 0.000 description 10
- 230000000717 retained effect Effects 0.000 description 10
- 230000008021 deposition Effects 0.000 description 9
- 101710092462 Alpha-hemolysin Proteins 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- 102000035160 transmembrane proteins Human genes 0.000 description 7
- 108091005703 transmembrane proteins Proteins 0.000 description 7
- 239000003446 ligand Substances 0.000 description 6
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 6
- 150000003904 phospholipids Chemical class 0.000 description 6
- 102000004310 Ion Channels Human genes 0.000 description 5
- 229910052782 aluminium Inorganic materials 0.000 description 5
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 239000002502 liposome Substances 0.000 description 5
- 229910021607 Silver chloride Inorganic materials 0.000 description 4
- DIOQZVSQGTUSAI-UHFFFAOYSA-N decane Chemical compound CCCCCCCCCC DIOQZVSQGTUSAI-UHFFFAOYSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000007876 drug discovery Methods 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 238000000206 photolithography Methods 0.000 description 3
- 229920000052 poly(p-xylylene) Polymers 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229920002120 photoresistant polymer Polymers 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 238000001878 scanning electron micrograph Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- SNKAWJBJQDLSFF-NVKMUCNASA-N 1,2-dioleoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/CCCCCCCC SNKAWJBJQDLSFF-NVKMUCNASA-N 0.000 description 1
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- VRBFTYUMFJWSJY-UHFFFAOYSA-N 28804-46-8 Chemical compound ClC1CC(C=C2)=CC=C2C(Cl)CC2=CC=C1C=C2 VRBFTYUMFJWSJY-UHFFFAOYSA-N 0.000 description 1
- 102000005416 ATP-Binding Cassette Transporters Human genes 0.000 description 1
- 108010006533 ATP-Binding Cassette Transporters Proteins 0.000 description 1
- 108010009551 Alamethicin Proteins 0.000 description 1
- 108010026389 Gramicidin Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- LGHSQOCGTJHDIL-UTXLBGCNSA-N alamethicin Chemical compound N([C@@H](C)C(=O)NC(C)(C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)NC(C)(C)C(=O)N[C@H](C(=O)NC(C)(C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)NC(C)(C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(=O)NC(C)(C)C(=O)NC(C)(C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](CO)CC=1C=CC=CC=1)C(C)C)C(=O)C(C)(C)NC(=O)[C@@H]1CCCN1C(=O)C(C)(C)NC(C)=O LGHSQOCGTJHDIL-UTXLBGCNSA-N 0.000 description 1
- 150000001338 aliphatic hydrocarbons Chemical class 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 125000001309 chloro group Chemical group Cl* 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
- 229910052731 fluorine Chemical group 0.000 description 1
- 125000001153 fluoro group Chemical group F* 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 229960004905 gramicidin Drugs 0.000 description 1
- ZWCXYZRRTRDGQE-SORVKSEFSA-N gramicidina Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CC=3C4=CC=CC=C4NC=3)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@H](NC(=O)[C@H](C)NC(=O)CNC(=O)[C@@H](NC=O)C(C)C)CC(C)C)C(=O)NCCO)=CNC2=C1 ZWCXYZRRTRDGQE-SORVKSEFSA-N 0.000 description 1
- 239000003228 hemolysin Substances 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
Landscapes
- Physical Or Chemical Processes And Apparatus (AREA)
Description
12 上部マイクロ流路チップ
14 下部マイクロ流路チップ
16 上部マイクロ流路
18 下部マイクロ流路
20 セパレーター
22 自己支持性フィルム
24 脂質二重膜
26 タンパク質分子
1. 透孔を有する自己支持性フィルムの作製
図3に模式的に示すフォトリソグラフィー法により、孔径800nmの透孔を有する、パラキシリレン系ポリマーから成る自己支持性フィルムを作製した。
図1及び図2に示す、上記したマイクロ流路デバイスを作成した。上部マイクロ流路チップ12、下部マイクロ流路チップ14及びセパレーター20はいずれもポリメチルメタクリレート(PMMA)で形成した。上部マイクロ流路チップ12及び下部マイクロ流路チップ14の寸法は、40mm x 25 mm x 3 mmであった。また、全てのマイクロ流路の幅及び深さは400μmであった。セパレーター20の3つの開口部の寸法は、直径1mmであり、この開口部に各自己支持性フィルム22の透孔が重なるように自己支持性フィルム22を載置した(図2参照)。さらに、上部マイクロ流路16及び下部マイクロ流路18中にAg/AgCl電極をそれぞれ配置し、これらの間に電圧をかけるパッチクランプアンプと接続できるようにした。上部マイクロ流路チップ12、下部マイクロ流路チップ14、セパレーター20を重ね合わせた状態で125℃で30分間熱処理し、これらを接着した。
Rp = (4ρL)/(πd2)
(式中、Rpは透孔の電気抵抗、ρは電解質の比抵抗、Lは透孔の長さ、dは透孔の孔径)
で表わされる理論値(非特許文献5)と概ね一致していた。
作製したマイクロ流路デバイスの上部マイクロ流路チップ12と下部マイクロ流路チップ14を重ね合わせた状態で、上部マイクロ流路16に、ポンプ(マイクロシリンジとチューブから成る手動式のもの、図示せず)により脂質溶液を流した。脂質溶液は、10mgのDPhPC(米国Avanti Polar Lipids社製)を1mLのn-デカンに溶解したものである。これにより、自己支持性フィルムの透孔に脂質二重膜が形成された。
Claims (4)
- 孔径が1μm以上の透孔を有する自己支持性フィルムを準備する工程と、該自己支持性フィルム上に被着可能な化合物を、蒸着により少なくとも前記透孔の内壁上に被着させ、それによって前記透孔の孔径を1μm未満に縮小する工程とを含む、孔径が1μm未満の透孔を有する自己支持性フィルムの製造方法。
- 縮小後の前記孔径が1nm〜900nmである請求項1記載の方法。
- 前記自己支持性フィルムと、蒸着される化合物とが同種の化合物である請求項1又は2記載の方法。
- 蒸着される前記化合物がパラキシリレン系モノマーである請求項3記載の方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010012300A JP5345078B2 (ja) | 2010-01-22 | 2010-01-22 | 脂質二重膜、それを形成するために用いられる自己支持性フィルム及びそれを具備するマイクロ流路デバイス |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010012300A JP5345078B2 (ja) | 2010-01-22 | 2010-01-22 | 脂質二重膜、それを形成するために用いられる自己支持性フィルム及びそれを具備するマイクロ流路デバイス |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2011149868A JP2011149868A (ja) | 2011-08-04 |
JP5345078B2 true JP5345078B2 (ja) | 2013-11-20 |
Family
ID=44536978
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2010012300A Expired - Fee Related JP5345078B2 (ja) | 2010-01-22 | 2010-01-22 | 脂質二重膜、それを形成するために用いられる自己支持性フィルム及びそれを具備するマイクロ流路デバイス |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP5345078B2 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014030382A (ja) * | 2012-08-03 | 2014-02-20 | Univ Of Tokyo | マイクロ流体デバイス及び脂質二重膜の形成方法 |
JP6376636B2 (ja) * | 2013-10-17 | 2018-08-22 | 地方独立行政法人神奈川県立産業技術総合研究所 | 脂質二重膜形成器具 |
WO2016004029A1 (en) * | 2014-06-30 | 2016-01-07 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | Systems and methods of preparing stabilized lipid assemblies |
JP7067699B2 (ja) | 2018-01-19 | 2022-05-16 | 地方独立行政法人神奈川県立産業技術総合研究所 | 脂質二重膜形成用隔壁及びその製造方法 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0818883B2 (ja) * | 1986-05-16 | 1996-02-28 | エヌオーケー株式会社 | 多孔質フィルタの製造法 |
JP3769622B2 (ja) * | 2003-09-22 | 2006-04-26 | 国立大学法人 東京大学 | 人工脂質膜の形成方法とそのための脂質平面膜形成装置 |
EP1547676A1 (en) * | 2003-12-24 | 2005-06-29 | Corning Incorporated | Porous membrane microstructure devices and methods of manufacture |
WO2005071405A1 (ja) * | 2004-01-21 | 2005-08-04 | Japan Science And Technology Agency | 膜タンパク質分析用平面脂質二重膜の形成方法とその装置 |
JP2006239663A (ja) * | 2005-03-07 | 2006-09-14 | Noritake Co Ltd | 水素ガス分離膜の製造方法 |
US8318520B2 (en) * | 2005-12-30 | 2012-11-27 | Lin Ming-Nung | Method of microminiaturizing a nano-structure |
US7777505B2 (en) * | 2006-05-05 | 2010-08-17 | University Of Utah Research Foundation | Nanopore platforms for ion channel recordings and single molecule detection and analysis |
JP5051841B2 (ja) * | 2007-11-29 | 2012-10-17 | 国立大学法人 東京大学 | 基板の微小孔上の人工脂質膜の作製方法及びその人工脂質膜保持基板 |
CN101971013B (zh) * | 2008-08-26 | 2013-06-19 | 松下电器产业株式会社 | 人工脂质膜形成方法和人工脂质膜形成装置 |
-
2010
- 2010-01-22 JP JP2010012300A patent/JP5345078B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JP2011149868A (ja) | 2011-08-04 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Han et al. | Nanopore arrays for stable and functional free‐standing lipid bilayers | |
Römer et al. | Channel activity of a viral transmembrane peptide in micro-BLMs: Vpu1-32 from HIV-1 | |
Khan et al. | Electrochemical impedance spectroscopy for black lipid membranes fused with channel protein supported on solid-state nanopore | |
JP5614642B2 (ja) | 脂質二重膜の形成方法及びそのための器具 | |
Tabaei et al. | Controlling the formation of phospholipid monolayer, bilayer, and intact vesicle layer on graphene | |
US8294007B2 (en) | Method of stabilization of functional nanoscale pores for device applications | |
JP5159808B2 (ja) | 脂質二分子膜基板 | |
Schmitt et al. | Electrically insulating pore-suspending membranes on highly ordered porous alumina obtained from vesicle spreading | |
Lazzara et al. | Separating attoliter-sized compartments using fluid pore-spanning lipid bilayers | |
Mey et al. | Biomimetic functionalization of porous substrates: towards model systems for cellular membranes | |
JP5345078B2 (ja) | 脂質二重膜、それを形成するために用いられる自己支持性フィルム及びそれを具備するマイクロ流路デバイス | |
Wolfrum et al. | Suspended nanoporous membranes as interfaces for neuronal biohybrid systems | |
Heitz et al. | Polymerized planar suspended lipid bilayers for single ion channel recordings: comparison of several dienoyl lipids | |
Baek et al. | Ion gating in nanopore electrode arrays with hierarchically organized pH-responsive block copolymer membranes | |
Maher et al. | Micron dimensioned cavity array supported lipid bilayers for the electrochemical investigation of ionophore activity | |
Movsesian et al. | Giant lipid vesicle formation using vapor-deposited charged porous polymers | |
US11892406B2 (en) | Method and device for assaying the interaction and dynamics of permeation of a molecule and a lipid bilayer | |
Sun et al. | Lipid membranes supported by planar porous substrates | |
JP6963284B2 (ja) | 脂質二重膜基板におけるリンカー層の制御方法、並びに、脂質二重膜基板及びその製造方法 | |
Hansen et al. | Large scale biomimetic membrane arrays | |
US10444244B2 (en) | Microfluidic array supporting a lipid bilayer assembly | |
Martin et al. | Planar bilayer measurements of alamethicin and gramicidin reconstituted in biomimetic block copolymers | |
Ahmed et al. | Rapid lipid bilayer membrane formation on Parylene coated apertures to perform ion channel analyses | |
JP6376636B2 (ja) | 脂質二重膜形成器具 | |
Zheng et al. | Generation of chip based microelectrochemical cell arrays for long-term and high-resolution recording of ionic currents through ion channel proteins |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20121204 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20130417 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20130514 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130712 |
|
RD02 | Notification of acceptance of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7422 Effective date: 20130712 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20130806 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20130813 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5345078 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
R360 | Written notification for declining of transfer of rights |
Free format text: JAPANESE INTERMEDIATE CODE: R360 |
|
R360 | Written notification for declining of transfer of rights |
Free format text: JAPANESE INTERMEDIATE CODE: R360 |
|
R371 | Transfer withdrawn |
Free format text: JAPANESE INTERMEDIATE CODE: R371 |
|
S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |