JP5265552B2 - 陰イオン交換を用いる核酸精製方法 - Google Patents

陰イオン交換を用いる核酸精製方法 Download PDF

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Publication number
JP5265552B2
JP5265552B2 JP2009529350A JP2009529350A JP5265552B2 JP 5265552 B2 JP5265552 B2 JP 5265552B2 JP 2009529350 A JP2009529350 A JP 2009529350A JP 2009529350 A JP2009529350 A JP 2009529350A JP 5265552 B2 JP5265552 B2 JP 5265552B2
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Prior art keywords
nucleic acid
genomic dna
resin
anion exchanger
eluent
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Japanese (ja)
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JP2010504738A (ja
JP2010504738A5 (enExample
Inventor
タッケラッパティ，スッダカール・ラオ
アンバット，ラジェッシュ
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Global Life Sciences Solutions USA LLC
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Amersham Biosciences Corp
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP2009529350A 2006-09-26 2007-09-19 陰イオン交換を用いる核酸精製方法 Active JP5265552B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US82691306P 2006-09-26 2006-09-26
US60/826,913 2006-09-26
PCT/US2007/078816 WO2008039664A1 (en) 2006-09-26 2007-09-19 Nucleic acid purification method using anion exchange

Publications (3)

Publication Number Publication Date
JP2010504738A JP2010504738A (ja) 2010-02-18
JP2010504738A5 JP2010504738A5 (enExample) 2011-10-20
JP5265552B2 true JP5265552B2 (ja) 2013-08-14

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ID=38640520

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2009529350A Active JP5265552B2 (ja) 2006-09-26 2007-09-19 陰イオン交換を用いる核酸精製方法

Country Status (5)

Country Link
US (1) US7655792B2 (enExample)
EP (1) EP2066792B1 (enExample)
JP (1) JP5265552B2 (enExample)
GB (1) GB2445442A (enExample)
WO (1) WO2008039664A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009100172A1 (en) 2008-02-07 2009-08-13 Ge Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
EP2315839A4 (en) * 2008-08-25 2012-03-21 Ge Healthcare Bio Sciences EASY LOADING AND ELONGATION METHOD FOR CLEANING GENOMIC DNA
DK2588609T3 (en) 2010-06-29 2018-03-19 Exscale Biospecimen Solutions Ab METHOD AND KIT FOR SEQUENTIAL ISOLATION OF NUCLEOTIDE SPECIES FROM A SAMPLE
JP6090985B2 (ja) * 2011-01-12 2017-03-08 積水メディカル株式会社 核酸鎖の分離方法
JP6061381B2 (ja) * 2011-01-12 2017-01-18 積水メディカル株式会社 一塩基多型の検出方法
CA2831613A1 (en) 2011-03-31 2012-10-04 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
EP4279610A3 (en) 2013-03-15 2024-01-03 ModernaTX, Inc. Ribonucleic acid purification
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
EP2971033B8 (en) 2013-03-15 2019-07-10 ModernaTX, Inc. Manufacturing methods for production of rna transcripts
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
EP3052511A4 (en) 2013-10-02 2017-05-31 Moderna Therapeutics, Inc. Polynucleotide molecules and uses thereof
US10286086B2 (en) 2014-06-19 2019-05-14 Modernatx, Inc. Alternative nucleic acid molecules and uses thereof
AU2015289656A1 (en) 2014-07-16 2017-02-16 Modernatx, Inc. Circular polynucleotides
EP4101930A1 (en) 2015-09-17 2022-12-14 ModernaTX, Inc. Polynucleotides containing a stabilizing tail region
WO2017049286A1 (en) 2015-09-17 2017-03-23 Moderna Therapeutics, Inc. Polynucleotides containing a morpholino linker
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
WO2021111418A1 (en) 2019-12-05 2021-06-10 Waters Technologies Corporation Polyphosphonic acids for improving recovery and minimizing system loss
SI3896159T1 (sl) 2020-04-17 2025-08-29 Sartorius Bia Separations D.O.O. Metoda čiščenja enoverižne rnk z uporabo anionskega izmenjevalca
US20220017887A1 (en) * 2020-07-14 2022-01-20 Waters Technologies Corporation Compositions, kits and methods useful for separating oligonucleotides from matrix components
EP4754251A1 (en) * 2023-08-02 2026-06-10 Merck Patent GmbH Method for purification of plasmid dna
WO2025257280A1 (en) * 2024-06-14 2025-12-18 Merck Patent Gmbh Method for purification of rna

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4034036C2 (de) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
US5866429A (en) * 1991-04-03 1999-02-02 Bloch; Will Precision and accuracy of anion-exchange separation of nucleic acids
DE4143639C2 (de) 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US5990301A (en) * 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
JP3523878B2 (ja) * 1994-03-11 2004-04-26 アボット・ラボラトリーズ ヘモグロビンの定量用無シアン化物試薬及び方法
SE9500183D0 (sv) * 1995-01-20 1995-01-20 Pharmacia Biotech Ab Method for the purifivation of short nucleic acids
SE9600590D0 (sv) * 1996-02-19 1996-02-19 Pharmacia Biotech Ab Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
US5707812A (en) * 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
SE9703532D0 (sv) * 1997-09-30 1997-09-30 Pharmacia Biotech Ab A process for the purification of plasmid DNA
EP0992583B1 (en) 1998-09-14 2003-03-26 QIAGEN GmbH Novel method for purifying covalently closed circular DNA
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
DE50008101D1 (de) 2000-02-16 2004-11-11 Macherey Nagel Gmbh & Co Hg Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens
EP1243649A1 (de) * 2001-03-23 2002-09-25 GeneScan Europe AG Verfahren zur Isolierung von DNA
JP2005520547A (ja) * 2002-03-21 2005-07-14 アベシア・バイオテクノロジー・インコーポレーテッド オリゴヌクレオチド及びその類似体のための精製方法
US7964343B2 (en) * 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture

Also Published As

Publication number Publication date
WO2008039664A1 (en) 2008-04-03
US20080076910A1 (en) 2008-03-27
EP2066792B1 (en) 2014-03-12
JP2010504738A (ja) 2010-02-18
GB2445442A (en) 2008-07-09
EP2066792A1 (en) 2009-06-10
US7655792B2 (en) 2010-02-02
GB0717591D0 (en) 2007-10-17

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