GB2445442A - Nucleic acid purification using anion exchange - Google Patents
Nucleic acid purification using anion exchange Download PDFInfo
- Publication number
- GB2445442A GB2445442A GB0717591A GB0717591A GB2445442A GB 2445442 A GB2445442 A GB 2445442A GB 0717591 A GB0717591 A GB 0717591A GB 0717591 A GB0717591 A GB 0717591A GB 2445442 A GB2445442 A GB 2445442A
- Authority
- GB
- United Kingdom
- Prior art keywords
- nucleic acid
- anion exchanger
- solution
- composition
- recovery
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000005349 anion exchange Methods 0.000 title description 3
- 238000001821 nucleic acid purification Methods 0.000 title description 2
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 85
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 84
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 84
- 238000010828 elution Methods 0.000 claims abstract description 61
- 238000000034 method Methods 0.000 claims abstract description 46
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims abstract description 40
- 238000011084 recovery Methods 0.000 claims abstract description 39
- 238000000746 purification Methods 0.000 claims abstract description 33
- 150000001450 anions Chemical class 0.000 claims abstract description 32
- 229910000027 potassium carbonate Inorganic materials 0.000 claims abstract description 20
- 239000000203 mixture Substances 0.000 claims abstract description 19
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- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 claims description 4
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- ZRALSGWEFCBTJO-UHFFFAOYSA-N anhydrous guanidine Natural products NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 abstract description 22
- 239000004475 Arginine Substances 0.000 abstract description 19
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- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 abstract description 17
- STIAPHVBRDNOAJ-UHFFFAOYSA-N carbamimidoylazanium;carbonate Chemical compound NC(N)=N.NC(N)=N.OC(O)=O STIAPHVBRDNOAJ-UHFFFAOYSA-N 0.000 abstract description 16
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 abstract description 8
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 abstract description 8
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 15
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- 229940113082 thymine Drugs 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US82691306P | 2006-09-26 | 2006-09-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB0717591D0 GB0717591D0 (en) | 2007-10-17 |
| GB2445442A true GB2445442A (en) | 2008-07-09 |
Family
ID=38640520
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB0717591A Withdrawn GB2445442A (en) | 2006-09-26 | 2007-09-10 | Nucleic acid purification using anion exchange |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7655792B2 (enExample) |
| EP (1) | EP2066792B1 (enExample) |
| JP (1) | JP5265552B2 (enExample) |
| GB (1) | GB2445442A (enExample) |
| WO (1) | WO2008039664A1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220017887A1 (en) * | 2020-07-14 | 2022-01-20 | Waters Technologies Corporation | Compositions, kits and methods useful for separating oligonucleotides from matrix components |
| US11912983B2 (en) | 2019-12-05 | 2024-02-27 | Waters Technologies Corporation | Polyanionic acids to improve recovery and minimize system loss |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009100172A1 (en) | 2008-02-07 | 2009-08-13 | Ge Healthcare Bio-Sciences Corp. | Isolation of dna, rna and protein from a single sample |
| EP2315839A4 (en) * | 2008-08-25 | 2012-03-21 | Ge Healthcare Bio Sciences | EASY LOADING AND ELONGATION METHOD FOR CLEANING GENOMIC DNA |
| DK2588609T3 (en) | 2010-06-29 | 2018-03-19 | Exscale Biospecimen Solutions Ab | METHOD AND KIT FOR SEQUENTIAL ISOLATION OF NUCLEOTIDE SPECIES FROM A SAMPLE |
| JP6090985B2 (ja) * | 2011-01-12 | 2017-03-08 | 積水メディカル株式会社 | 核酸鎖の分離方法 |
| JP6061381B2 (ja) * | 2011-01-12 | 2017-01-18 | 積水メディカル株式会社 | 一塩基多型の検出方法 |
| CA2831613A1 (en) | 2011-03-31 | 2012-10-04 | Moderna Therapeutics, Inc. | Delivery and formulation of engineered nucleic acids |
| EP4279610A3 (en) | 2013-03-15 | 2024-01-03 | ModernaTX, Inc. | Ribonucleic acid purification |
| WO2014144767A1 (en) | 2013-03-15 | 2014-09-18 | Moderna Therapeutics, Inc. | Ion exchange purification of mrna |
| EP2971033B8 (en) | 2013-03-15 | 2019-07-10 | ModernaTX, Inc. | Manufacturing methods for production of rna transcripts |
| US10077439B2 (en) | 2013-03-15 | 2018-09-18 | Modernatx, Inc. | Removal of DNA fragments in mRNA production process |
| EP3052511A4 (en) | 2013-10-02 | 2017-05-31 | Moderna Therapeutics, Inc. | Polynucleotide molecules and uses thereof |
| US10286086B2 (en) | 2014-06-19 | 2019-05-14 | Modernatx, Inc. | Alternative nucleic acid molecules and uses thereof |
| AU2015289656A1 (en) | 2014-07-16 | 2017-02-16 | Modernatx, Inc. | Circular polynucleotides |
| EP4101930A1 (en) | 2015-09-17 | 2022-12-14 | ModernaTX, Inc. | Polynucleotides containing a stabilizing tail region |
| WO2017049286A1 (en) | 2015-09-17 | 2017-03-23 | Moderna Therapeutics, Inc. | Polynucleotides containing a morpholino linker |
| US12385034B2 (en) | 2016-06-24 | 2025-08-12 | Modernatx, Inc. | Methods and apparatus for filtration |
| SI3896159T1 (sl) | 2020-04-17 | 2025-08-29 | Sartorius Bia Separations D.O.O. | Metoda čiščenja enoverižne rnk z uporabo anionskega izmenjevalca |
| EP4754251A1 (en) * | 2023-08-02 | 2026-06-10 | Merck Patent GmbH | Method for purification of plasmid dna |
| WO2025257280A1 (en) * | 2024-06-14 | 2025-12-18 | Merck Patent Gmbh | Method for purification of rna |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997029825A1 (en) * | 1996-02-19 | 1997-08-21 | Amersham Pharmacia Biotech Aktiebolag | Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix |
| US6020186A (en) * | 1990-10-26 | 2000-02-01 | Qiagen Gmbh | Device and process for isolating nucleic acids from cell suspensions |
| WO2000069872A2 (en) * | 1999-05-14 | 2000-11-23 | Promega Corporation | pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS |
| EP1243649A1 (de) * | 2001-03-23 | 2002-09-25 | GeneScan Europe AG | Verfahren zur Isolierung von DNA |
| WO2003080834A2 (en) * | 2002-03-21 | 2003-10-02 | Avecia Biotechnology Inc. | Purification methods for oligonucleotides and their analogs |
| WO2004101809A2 (en) * | 2003-05-13 | 2004-11-25 | Isis Pharmaceuticals, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5866429A (en) * | 1991-04-03 | 1999-02-02 | Bloch; Will | Precision and accuracy of anion-exchange separation of nucleic acids |
| DE4143639C2 (de) | 1991-12-02 | 2002-10-24 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren |
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| US5990301A (en) * | 1994-02-07 | 1999-11-23 | Qiagen Gmbh | Process for the separation and purification of nucleic acids from biological sources |
| JP3523878B2 (ja) * | 1994-03-11 | 2004-04-26 | アボット・ラボラトリーズ | ヘモグロビンの定量用無シアン化物試薬及び方法 |
| SE9500183D0 (sv) * | 1995-01-20 | 1995-01-20 | Pharmacia Biotech Ab | Method for the purifivation of short nucleic acids |
| US5707812A (en) * | 1996-08-06 | 1998-01-13 | Vical Incorporated | Purification of plasmid DNA during column chromatography |
| SE9703532D0 (sv) * | 1997-09-30 | 1997-09-30 | Pharmacia Biotech Ab | A process for the purification of plasmid DNA |
| EP0992583B1 (en) | 1998-09-14 | 2003-03-26 | QIAGEN GmbH | Novel method for purifying covalently closed circular DNA |
| DE50008101D1 (de) | 2000-02-16 | 2004-11-11 | Macherey Nagel Gmbh & Co Hg | Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens |
-
2007
- 2007-09-10 GB GB0717591A patent/GB2445442A/en not_active Withdrawn
- 2007-09-19 JP JP2009529350A patent/JP5265552B2/ja active Active
- 2007-09-19 WO PCT/US2007/078816 patent/WO2008039664A1/en not_active Ceased
- 2007-09-19 EP EP07853559.8A patent/EP2066792B1/en active Active
- 2007-09-19 US US11/857,468 patent/US7655792B2/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6020186A (en) * | 1990-10-26 | 2000-02-01 | Qiagen Gmbh | Device and process for isolating nucleic acids from cell suspensions |
| WO1997029825A1 (en) * | 1996-02-19 | 1997-08-21 | Amersham Pharmacia Biotech Aktiebolag | Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix |
| WO2000069872A2 (en) * | 1999-05-14 | 2000-11-23 | Promega Corporation | pH DEPENDENT ION EXCHANGE MATRIX AND METHOD OF USE IN THE ISOLATION OF NUCLEIC ACIDS |
| EP1243649A1 (de) * | 2001-03-23 | 2002-09-25 | GeneScan Europe AG | Verfahren zur Isolierung von DNA |
| WO2003080834A2 (en) * | 2002-03-21 | 2003-10-02 | Avecia Biotechnology Inc. | Purification methods for oligonucleotides and their analogs |
| WO2004101809A2 (en) * | 2003-05-13 | 2004-11-25 | Isis Pharmaceuticals, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
Non-Patent Citations (1)
| Title |
|---|
| Journal of Chromatography B (2005); Vol 822, pp 54-60, "Application of superparamagnetic...", Chiang et al * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11912983B2 (en) | 2019-12-05 | 2024-02-27 | Waters Technologies Corporation | Polyanionic acids to improve recovery and minimize system loss |
| US12359191B2 (en) | 2019-12-05 | 2025-07-15 | Waters Technologies Corporation | Polyanionic acids to improve recovery and minimize system loss |
| US20220017887A1 (en) * | 2020-07-14 | 2022-01-20 | Waters Technologies Corporation | Compositions, kits and methods useful for separating oligonucleotides from matrix components |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2008039664A1 (en) | 2008-04-03 |
| US20080076910A1 (en) | 2008-03-27 |
| EP2066792B1 (en) | 2014-03-12 |
| JP2010504738A (ja) | 2010-02-18 |
| EP2066792A1 (en) | 2009-06-10 |
| US7655792B2 (en) | 2010-02-02 |
| GB0717591D0 (en) | 2007-10-17 |
| JP5265552B2 (ja) | 2013-08-14 |
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| Date | Code | Title | Description |
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| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |