JP5249777B2 - サンプルの蛍光発光を測定するための方法および装置ならびにその使用 - Google Patents
サンプルの蛍光発光を測定するための方法および装置ならびにその使用 Download PDFInfo
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- JP5249777B2 JP5249777B2 JP2008544709A JP2008544709A JP5249777B2 JP 5249777 B2 JP5249777 B2 JP 5249777B2 JP 2008544709 A JP2008544709 A JP 2008544709A JP 2008544709 A JP2008544709 A JP 2008544709A JP 5249777 B2 JP5249777 B2 JP 5249777B2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Description
Claims (13)
- サンプル(40、47)をサンプル(40)内で蛍光(22、28)を励起させるために適した波長の光(25)で照射し、サンプル(40、47)によって発せられる蛍光(28)を受信器(24)にて受光して測定信号へと変換し、周囲の影響を補償するために、さらに基準光(32)を受信器(24)へともたらして同様に基準測定信号へと変換する、サンプルの蛍光発光を測定するための方法であって、
サンプル(40、47)へと進入する励起光(25)およびサンプル(40、47)から出る蛍光(27、28)の光学経路が、励起光(25)をもたらす光源(21)及び基準光(32)をもたらす別個の光源(31)の両方と受信器(24)との間において、励起光と同じ波長を有する基準光の光学経路から分離されており、
基準光(32)が、前記励起光(25)をもたらす光源(21)と同一である前記別個の光源(31)によってもたらされることを特徴とする、方法。 - 光学経路(25、27、28、32)の分離が、光学フィルタ(32)によって達成されることを特徴とする、請求項1に記載の方法。
- 基準光(32)が、光導体を通って受信器(24)へともたらされることを特徴とする、請求項1または2に記載の方法。
- 励起光の光源(21)および基準光の光源(31)が、共通の変調器によって駆動されることを特徴とする、請求項1から3に記載の方法。
- 受信器(24)によって発せられる測定信号が、それ自身は知られている様式で、増幅器(11)において増幅され、次いで信号処理ユニット(12)において処理され、表示されることを特徴とする、請求項1から4のいずれか一項に記載の方法。
- サンプル内で蛍光(27、28)を励起させるために適した波長の光を発するための光源(21)と、サンプルによって発せられる蛍光(28)を受信して、測定信号へと変換する受信器(24)とを含み、さらに基準光(32)を、周囲の影響を補償するために、受信器(24)へと供給して、受信器(24)によって基準測定信号へと変換することができる、サンプルの蛍光発光を測定するための装置であって、
サンプル(40、47)へと進入する励起光(25)およびサンプルから出る蛍光(27、28)の光学経路が、2つの同一かつ別個の光源(21、31)と受信器(24)との間において、励起光と同じ波長を有する基準光の光学経路(32)から分離されており、
前記2つの同一かつ別個の光源(21、31)が、蛍光発光の励起のためにサンプル(40)を照射するための光の生成、および基準光の生成のために設けられていることを特徴とする、装置。 - 光学経路(25、27、28、32)の分離のために光学フィルタ(23)が使用されることを特徴とする、請求項6に記載の装置。
- 2つの光源(21、31)のために、共通の周波数変調器(10)が設けられていることを特徴とする、請求項6または7に記載の装置。
- ファイバケーブルである光導体(42、48)が、基準光を受信器へと供給するために設けられていることを特徴とする、請求項6から8のいずれか一項に記載の装置。
- それ自身は知られている様式で、増幅器(11)、および評価/処理ユニット(12)、ならびに表示ユニット(17)が、受信器(24)の下流に配置されていることを特徴とする、請求項6から9のいずれか一項に記載の装置。
- 光源(21、31)が、LEDによって構成されていることを特徴とする、請求項6から10のいずれか一項に記載の装置。
- バイオリアクタ、化学的および/または生化学的分析、あるいは医療診断における請求項1から5のいずれか一項に記載の方法の使用。
- バイオリアクタ、化学的および/または生化学的分析、あるいは医療診断における請求項6から11のいずれか一項に記載の装置の使用。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT0201405A AT502194B1 (de) | 2005-12-16 | 2005-12-16 | Verfahren und vorrichtung zur bestimmung der fluoreszenz einer probe sowie verwendung derselben |
ATA2014/2005 | 2005-12-16 | ||
PCT/AT2006/000517 WO2007068021A1 (de) | 2005-12-16 | 2006-12-15 | Verfahren und vorrichtung zur bestimmung der fluoreszenz einer probe sowie verwendung derselben |
Publications (2)
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JP2009519441A JP2009519441A (ja) | 2009-05-14 |
JP5249777B2 true JP5249777B2 (ja) | 2013-07-31 |
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JP2008544709A Expired - Fee Related JP5249777B2 (ja) | 2005-12-16 | 2006-12-15 | サンプルの蛍光発光を測定するための方法および装置ならびにその使用 |
Country Status (6)
Country | Link |
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US (1) | US7943910B2 (ja) |
EP (1) | EP1960760B1 (ja) |
JP (1) | JP5249777B2 (ja) |
AT (1) | AT502194B1 (ja) |
ES (1) | ES2690570T3 (ja) |
WO (1) | WO2007068021A1 (ja) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
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JP5443404B2 (ja) * | 2011-02-08 | 2014-03-19 | 三井造船株式会社 | 蛍光検出装置、蛍光検出装置の診断方法、および蛍光検出方法 |
DE102011002080B4 (de) * | 2011-04-15 | 2016-05-04 | Lre Medical Gmbh | Vorrichtung und Verfahren zur Bestimmung der Konzentration von Fluorophoren in einer Probe |
CN102914525B (zh) * | 2012-04-10 | 2016-06-01 | 广东工业大学 | 基于光学加法外差调制的新型荧光寿命显微成像装置及方法 |
CN103234950B (zh) * | 2013-05-13 | 2015-01-21 | 上海通微分析技术有限公司 | 并行双光路激光诱导荧光光谱仪 |
JP7026337B2 (ja) * | 2017-08-29 | 2022-02-28 | パナソニックIpマネジメント株式会社 | 光観測装置 |
DE102022122092A1 (de) | 2022-08-31 | 2024-02-29 | Pyroscience Gmbh | Referenz-Messung |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
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US4022529A (en) | 1975-12-11 | 1977-05-10 | White John U | Feature extraction system for extracting a predetermined feature from a signal |
CA1068508A (en) * | 1975-12-11 | 1979-12-25 | John U. White | Fluorescence spectrophotometer |
DE2622296C2 (de) * | 1976-05-19 | 1984-12-06 | Hoechst Ag, 6230 Frankfurt | Stabilisierter roter Phosphor sowie Verfahren zu seiner Herstellung |
GB1596522A (en) | 1977-10-20 | 1981-08-26 | Int Diagnostic Tech | Apparatus for fluorometric assay |
FI843409A0 (fi) | 1984-08-29 | 1984-08-29 | Labsystems Oy | Fluorometer. |
DD229220A1 (de) | 1984-11-22 | 1985-10-30 | Adw Ddr Inst Optik | Photodetektoranordnung fuer die spektroskopie |
JP2680398B2 (ja) * | 1989-03-08 | 1997-11-19 | 浜松ホトニクス株式会社 | 光波形測定装置 |
US5196709A (en) * | 1991-05-03 | 1993-03-23 | University Of Maryland Systems | Fluorometry method and apparatus using a semiconductor laser diode as a light source |
DE19849585C2 (de) | 1998-10-27 | 2001-06-28 | Fraunhofer Ges Forschung | Vorrichtung und Verfahren zur Bestimmung der Fluoreszenz einer Probe |
US6157037A (en) * | 1998-12-04 | 2000-12-05 | Photosense, Llc | Sensing device and method for measuring emission time delay during irradiation of targeted samples |
US6673626B1 (en) * | 2000-10-31 | 2004-01-06 | Science & Technology Corporation University Of New Mexico | Optoelectronic circuit for detecting changes in fluorescence lifetime |
WO2005033746A2 (en) * | 2003-09-29 | 2005-04-14 | Photosense L.L.C. | Frequency domain luminescence instrumentation |
GB0416732D0 (en) * | 2004-07-27 | 2004-09-01 | Precisense As | A method and apparatus for measuring the phase shift induced in a light signal by a sample |
US8455844B2 (en) * | 2009-03-11 | 2013-06-04 | Colorado State University Research Foundation | System and method for time-division multiplexed optical sensing of biosensors |
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2005
- 2005-12-16 AT AT0201405A patent/AT502194B1/de not_active IP Right Cessation
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- 2006-12-15 ES ES06817490.3T patent/ES2690570T3/es active Active
- 2006-12-15 WO PCT/AT2006/000517 patent/WO2007068021A1/de active Application Filing
- 2006-12-15 EP EP06817490.3A patent/EP1960760B1/de not_active Not-in-force
- 2006-12-15 JP JP2008544709A patent/JP5249777B2/ja not_active Expired - Fee Related
- 2006-12-15 US US12/086,004 patent/US7943910B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
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WO2007068021A1 (de) | 2007-06-21 |
ES2690570T3 (es) | 2018-11-21 |
EP1960760B1 (de) | 2018-07-11 |
AT502194A4 (de) | 2007-02-15 |
EP1960760A1 (de) | 2008-08-27 |
JP2009519441A (ja) | 2009-05-14 |
US20090283681A1 (en) | 2009-11-19 |
US7943910B2 (en) | 2011-05-17 |
AT502194B1 (de) | 2007-02-15 |
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