JP5241493B2 - Ｄｎａ結合タンパク質−ポリメラーゼのキメラ - Google Patents

Ｄｎａ結合タンパク質−ポリメラーゼのキメラ Download PDF

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Publication number
JP5241493B2
JP5241493B2 JP2008521706A JP2008521706A JP5241493B2 JP 5241493 B2 JP5241493 B2 JP 5241493B2 JP 2008521706 A JP2008521706 A JP 2008521706A JP 2008521706 A JP2008521706 A JP 2008521706A JP 5241493 B2 JP5241493 B2 JP 5241493B2
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Japan
Prior art keywords
dna polymerase
dna
polymerase
pcr
amino acid
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Expired - Fee Related
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JP2008521706A
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Japanese (ja)
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JP2009501530A5 (enExample
JP2009501530A (ja
Inventor
マイケル ボーンズ、
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Agilent Technologies Inc
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Agilent Technologies Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2008521706A 2005-07-15 2006-07-17 Ｄｎａ結合タンパク質−ポリメラーゼのキメラ Expired - Fee Related JP5241493B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US69993705P 2005-07-15 2005-07-15
US60/699,937 2005-07-15
PCT/US2006/027739 WO2007011891A2 (en) 2005-07-15 2006-07-17 Dna binding protein-polymerase chimeras

Publications (3)

Publication Number Publication Date
JP2009501530A JP2009501530A (ja) 2009-01-22
JP2009501530A5 JP2009501530A5 (enExample) 2009-09-03
JP5241493B2 true JP5241493B2 (ja) 2013-07-17

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Family Applications (1)

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JP2008521706A Expired - Fee Related JP5241493B2 (ja) 2005-07-15 2006-07-17 Ｄｎａ結合タンパク質−ポリメラーゼのキメラ

Country Status (5)

Country Link
US (1) US9040276B2 (enExample)
EP (1) EP1910534A4 (enExample)
JP (1) JP5241493B2 (enExample)
CA (1) CA2615151A1 (enExample)
WO (1) WO2007011891A2 (enExample)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2240576B1 (en) * 2008-01-11 2012-10-03 Genesys Ltd Cren7 chimeric protein
GB0803628D0 (en) * 2008-02-28 2008-04-02 Genesys Ltd Enzyme
GB0804721D0 (en) * 2008-03-14 2008-04-16 Genesys Ltd Enzyme
GB0804722D0 (en) * 2008-03-14 2008-04-16 Genesys Ltd Enzyme
CA2749093C (en) * 2009-01-08 2019-03-26 Bio-Rad Laboratories, Inc. Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions
ES2359058B1 (es) * 2009-07-02 2012-03-27 Consejo Superior De Investigaciones Cient�?Ficas (Csic) Quimera de adn polimerasa del fago ph1 29.
EP2478014A4 (en) * 2009-09-16 2013-11-27 Univ Massey FUSION POLYPEPTIDES AND USES THEREOF
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
FR2974816A1 (fr) 2011-05-04 2012-11-09 Centre Nat Rech Scient Methode de generation de proteines
US8470573B2 (en) 2011-06-21 2013-06-25 Bio-Rad Laboratories, Inc. Hybrid polymerases having the ability to produce long amplicons
US9109226B2 (en) 2011-09-01 2015-08-18 New England Biolabs, Inc. Synthetic nucleic acids for polymerization reactions
JP6490576B2 (ja) * 2012-04-12 2019-03-27 ニユー・イングランド・バイオレイブス・インコーポレイテツド 重合反応のための合成核酸
CN105452451B (zh) 2013-12-06 2020-06-05 生物辐射实验室股份有限公司 融合聚合酶
US10273511B2 (en) * 2014-06-30 2019-04-30 Wisconsin Alumni Research Foundation Enzymes for producing non-straight-chain fatty acids
US10131898B2 (en) 2014-07-22 2018-11-20 Bio-Rad Laboratories, Inc. Buffers for use with polymerases
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
CN113584015B (zh) * 2014-08-27 2025-05-02 新英格兰生物实验室公司 合成子的形成
EP3387002A4 (en) 2015-12-07 2019-04-24 Bio-Rad Laboratories, Inc. DIM REVERSE TRANSCRIPTASE
CN107841492A (zh) * 2016-09-21 2018-03-27 北京擎科新业生物技术有限公司 一种增强型Pfu DNA聚合酶及相关应用
US20200002689A1 (en) 2017-02-13 2020-01-02 Qiagen Sciences, Llc Polymerase enzyme from 9°n
EP3632924A1 (en) * 2018-10-07 2020-04-08 Affilogic Binders for inhibiting formation of multimeric proteins
CN109266628B (zh) * 2018-10-09 2020-04-14 南京市胸科医院 一种融合的TaqDNA聚合酶及其应用
CN109679932B (zh) * 2018-12-05 2020-03-17 广州奇辉生物科技有限公司 一种dna聚合酶、重组载体及它们的制备方法和应用
CN111718418B (zh) * 2019-03-19 2021-08-27 华东师范大学 一种增强基因编辑的融合蛋白及其应用
US11597751B2 (en) 2019-05-16 2023-03-07 Massachusetts Institute Of Technology Glycan-binding proteins and related compositions and methods
CN111690626B (zh) * 2020-07-02 2021-03-26 南京诺唯赞生物科技股份有限公司 一种融合型Taq DNA聚合酶及其制备方法和应用
CN113774039B (zh) * 2021-09-07 2022-06-21 北京擎科生物科技有限公司 重组型dna聚合酶及其应用
WO2023082266A1 (zh) * 2021-11-15 2023-05-19 深圳华大生命科学研究院 嵌合dna聚合酶及其应用
KR20250035622A (ko) * 2022-06-10 2025-03-12 엘리먼트 바이오사이언스, 인크. 열 안정성이 개선된 조작된 폴리머라제

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
AU2003256794B2 (en) * 2002-07-25 2008-07-31 Bio-Rad Laboratories, Inc. Hybrid protein methods and compositions
US7666645B2 (en) * 2002-10-23 2010-02-23 Bio-Rad Laboratories, Inc. Sso7-polymerase conjugate proteins
US7960157B2 (en) * 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
EP2194123B1 (en) 2003-03-25 2012-08-22 Stratagene California DNA polymerase fusions and uses thereof
WO2006074233A2 (en) * 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification

Also Published As

Publication number Publication date
WO2007011891A3 (en) 2008-01-24
EP1910534A2 (en) 2008-04-16
EP1910534A4 (en) 2010-03-10
US9040276B2 (en) 2015-05-26
US20070141591A1 (en) 2007-06-21
JP2009501530A (ja) 2009-01-22
WO2007011891A2 (en) 2007-01-25
CA2615151A1 (en) 2007-01-25

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