CA2615151A1 - Dna binding protein-polymerase chimeras - Google Patents
Dna binding protein-polymerase chimeras Download PDFInfo
- Publication number
- CA2615151A1 CA2615151A1 CA002615151A CA2615151A CA2615151A1 CA 2615151 A1 CA2615151 A1 CA 2615151A1 CA 002615151 A CA002615151 A CA 002615151A CA 2615151 A CA2615151 A CA 2615151A CA 2615151 A1 CA2615151 A1 CA 2615151A1
- Authority
- CA
- Canada
- Prior art keywords
- polymerase
- dna polymerase
- dna
- chimeric
- binding domain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US69993705P | 2005-07-15 | 2005-07-15 | |
| US60/699,937 | 2005-07-15 | ||
| PCT/US2006/027739 WO2007011891A2 (en) | 2005-07-15 | 2006-07-17 | Dna binding protein-polymerase chimeras |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2615151A1 true CA2615151A1 (en) | 2007-01-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002615151A Abandoned CA2615151A1 (en) | 2005-07-15 | 2006-07-17 | Dna binding protein-polymerase chimeras |
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| Country | Link |
|---|---|
| US (1) | US9040276B2 (enExample) |
| EP (1) | EP1910534A4 (enExample) |
| JP (1) | JP5241493B2 (enExample) |
| CA (1) | CA2615151A1 (enExample) |
| WO (1) | WO2007011891A2 (enExample) |
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| EP2240576B1 (en) * | 2008-01-11 | 2012-10-03 | Genesys Ltd | Cren7 chimeric protein |
| GB0803628D0 (en) * | 2008-02-28 | 2008-04-02 | Genesys Ltd | Enzyme |
| GB0804721D0 (en) * | 2008-03-14 | 2008-04-16 | Genesys Ltd | Enzyme |
| GB0804722D0 (en) * | 2008-03-14 | 2008-04-16 | Genesys Ltd | Enzyme |
| CA2749093C (en) * | 2009-01-08 | 2019-03-26 | Bio-Rad Laboratories, Inc. | Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions |
| ES2359058B1 (es) * | 2009-07-02 | 2012-03-27 | Consejo Superior De Investigaciones Cient�?Ficas (Csic) | Quimera de adn polimerasa del fago ph1 29. |
| EP2478014A4 (en) * | 2009-09-16 | 2013-11-27 | Univ Massey | FUSION POLYPEPTIDES AND USES THEREOF |
| US8715987B2 (en) | 2011-05-02 | 2014-05-06 | New England Biolabs, Inc. | Solubilized phospholipids for stabilizing nucleic acid polymerases |
| FR2974816A1 (fr) | 2011-05-04 | 2012-11-09 | Centre Nat Rech Scient | Methode de generation de proteines |
| US8470573B2 (en) | 2011-06-21 | 2013-06-25 | Bio-Rad Laboratories, Inc. | Hybrid polymerases having the ability to produce long amplicons |
| US9109226B2 (en) | 2011-09-01 | 2015-08-18 | New England Biolabs, Inc. | Synthetic nucleic acids for polymerization reactions |
| JP6490576B2 (ja) * | 2012-04-12 | 2019-03-27 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | 重合反応のための合成核酸 |
| CN105452451B (zh) | 2013-12-06 | 2020-06-05 | 生物辐射实验室股份有限公司 | 融合聚合酶 |
| US10273511B2 (en) * | 2014-06-30 | 2019-04-30 | Wisconsin Alumni Research Foundation | Enzymes for producing non-straight-chain fatty acids |
| US10131898B2 (en) | 2014-07-22 | 2018-11-20 | Bio-Rad Laboratories, Inc. | Buffers for use with polymerases |
| US9963687B2 (en) | 2014-08-27 | 2018-05-08 | New England Biolabs, Inc. | Fusion polymerase and method for using the same |
| CN113584015B (zh) * | 2014-08-27 | 2025-05-02 | 新英格兰生物实验室公司 | 合成子的形成 |
| EP3387002A4 (en) | 2015-12-07 | 2019-04-24 | Bio-Rad Laboratories, Inc. | DIM REVERSE TRANSCRIPTASE |
| CN107841492A (zh) * | 2016-09-21 | 2018-03-27 | 北京擎科新业生物技术有限公司 | 一种增强型Pfu DNA聚合酶及相关应用 |
| US20200002689A1 (en) | 2017-02-13 | 2020-01-02 | Qiagen Sciences, Llc | Polymerase enzyme from 9°n |
| EP3632924A1 (en) * | 2018-10-07 | 2020-04-08 | Affilogic | Binders for inhibiting formation of multimeric proteins |
| CN109266628B (zh) * | 2018-10-09 | 2020-04-14 | 南京市胸科医院 | 一种融合的TaqDNA聚合酶及其应用 |
| CN109679932B (zh) * | 2018-12-05 | 2020-03-17 | 广州奇辉生物科技有限公司 | 一种dna聚合酶、重组载体及它们的制备方法和应用 |
| CN111718418B (zh) * | 2019-03-19 | 2021-08-27 | 华东师范大学 | 一种增强基因编辑的融合蛋白及其应用 |
| US11597751B2 (en) | 2019-05-16 | 2023-03-07 | Massachusetts Institute Of Technology | Glycan-binding proteins and related compositions and methods |
| CN111690626B (zh) * | 2020-07-02 | 2021-03-26 | 南京诺唯赞生物科技股份有限公司 | 一种融合型Taq DNA聚合酶及其制备方法和应用 |
| CN113774039B (zh) * | 2021-09-07 | 2022-06-21 | 北京擎科生物科技有限公司 | 重组型dna聚合酶及其应用 |
| WO2023082266A1 (zh) * | 2021-11-15 | 2023-05-19 | 深圳华大生命科学研究院 | 嵌合dna聚合酶及其应用 |
| KR20250035622A (ko) * | 2022-06-10 | 2025-03-12 | 엘리먼트 바이오사이언스, 인크. | 열 안정성이 개선된 조작된 폴리머라제 |
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| US6627424B1 (en) | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
| AU2003256794B2 (en) * | 2002-07-25 | 2008-07-31 | Bio-Rad Laboratories, Inc. | Hybrid protein methods and compositions |
| US7666645B2 (en) * | 2002-10-23 | 2010-02-23 | Bio-Rad Laboratories, Inc. | Sso7-polymerase conjugate proteins |
| US7960157B2 (en) * | 2002-12-20 | 2011-06-14 | Agilent Technologies, Inc. | DNA polymerase blends and uses thereof |
| EP2194123B1 (en) | 2003-03-25 | 2012-08-22 | Stratagene California | DNA polymerase fusions and uses thereof |
| WO2006074233A2 (en) * | 2005-01-06 | 2006-07-13 | Applera Corporation | Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification |
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2006
- 2006-07-17 WO PCT/US2006/027739 patent/WO2007011891A2/en not_active Ceased
- 2006-07-17 EP EP06787626A patent/EP1910534A4/en not_active Withdrawn
- 2006-07-17 US US11/488,535 patent/US9040276B2/en not_active Expired - Fee Related
- 2006-07-17 CA CA002615151A patent/CA2615151A1/en not_active Abandoned
- 2006-07-17 JP JP2008521706A patent/JP5241493B2/ja not_active Expired - Fee Related
Also Published As
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|---|---|
| WO2007011891A3 (en) | 2008-01-24 |
| EP1910534A2 (en) | 2008-04-16 |
| EP1910534A4 (en) | 2010-03-10 |
| US9040276B2 (en) | 2015-05-26 |
| US20070141591A1 (en) | 2007-06-21 |
| JP2009501530A (ja) | 2009-01-22 |
| JP5241493B2 (ja) | 2013-07-17 |
| WO2007011891A2 (en) | 2007-01-25 |
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